Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
Version 001 12/02/2016
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……………………….…
CODE OF PRACTICE
for the use of the
PATHOGEN IMAGING FACILITY
UNIVERSITY OF EDINBURGH
ASHWORTH LABORATORIES,
KING’S BUILDINGS
Last updated: 26th September 2010
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
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Contents
Section
Main
User Guide
Form 1
Appendix 1
Appendix 2
Appendix 3
Appendix 4a
Appendix 4b
Appendix 5
Appendix 6a
Appendix 6b
Appendix 7
Appendix 8
Appendix 9
Page
Locale, Management, Type of Work, General
arrangements
FlowChart
Biosafety questionnaire for Flow Cytometry
analysis
Standard Operating procedures I (non
hazardous samples
Training Record I (List of authorized users for
non-hazardous samples)
Standard Operating procedures II (samples
requiring CL2)
Training Record II (for handling samples
requiring CL2)
List of authorized users for work requiring CL2
Standard Operating procedures III (samples
requiring CL3)
Training Record III (for handling samples
requiring CL3)
List of authorized users for work requiring CL3
CL3 work Decommissioning check list
List of standard microscope operating
instructions.
Charges
3
8
9
10
11
12
13
14
15
17
18
19
20
21
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
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LOCATION
The Room number of the Facility is B.64 (Ashworth 3, Basement room 64).
MANAGEMENT
This is a central shared facility for the School of Biological Sciences and the Centre for
Infectious Disease (CID) which also may be used by researcher’s outwith SBS and UoE.
The Head of School has delegated overall responsibility for the facility to an assigned
Responsible Principal Investigator (PI). Day to day management and operation of the
Confocal Microscopy Facility is under the responsibility of the Facility Manager. Advice
on safety matters may be obtained from the Biological Safety Advisor of the Ashworth
Laboratories or the School Biological Safety Officer/Safety Adviser.
At the present time the respective staff are:
Function
Facility Manager
Responsible PI
Ashworth Safety Adviser
School Safety Adviser
Name
Dr Dianne Murray
Prof Keith Mathews
Dr David Cavanagh
N.N.
Contact
51 3658
51 3639
50 5459
50 7746
Maintenance Procedures
Maintenance procedures for all major pieces of equipment are detailed elsewhere and are
the sole responsibility of the Facility Manager or the Responsible PI. Any problems with
any equipment or the operation of the Facility room should be reported to them.
It is the responsibility of the Facility Manager or the Responsible PI to issue and sign
permits of work and release certificates for equipment should this become necessary (cf.
http://www.safety.ed.ac.uk/resources/General/Lab_permit_to_work.pdf ). Users must
not allow maintenance or repair staff (contractors or University staff) to access the
facility unless they have a permit to work issued by the Facility Manager or the
Responsible PI.
Cleaners will be instructed by the Facility Manager, who will organise cleaning and
general disinfection of the facility. Guidance for cleaners is available from the safety
website and will be communicated to them by the Facility Manager before they are
given
access
to
the
facility
(c.f.
http://www.safety.ed.ac.uk/resources/Bio/Guidance/CL/Cleaning_Labs.shtm ).
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TYPE OF WORK
The work carried out in this laboratory is general and confocal microscopical analysis of
fixed and live samples. Images will be acquired and data processed for export. Fixed
samples analysed typically require only containment level 1 precautions (CL1).
However, live samples may contain pathogens in hazard group 2 (HG2) and some
samples from human sources may potentially contain pathogens including blood borne
viruses in HG3 but determined by risk assessment to be low risk for the presence of
these pathogens and for these CL2 precautions are required. Therefore, the facility will
routinely be operated observing CL2 precautions.
Occasionally, use of the facility will be dedicated to samples known to contain
particular pathogens in hazard group 3 (mainly parasites) which can be handled at CL3
with certain derogations Any of these samples could contain materials that have been
genetically modified..
The facility is not suitable for handling other high risk samples requiring full CL3
precautions.
All users must comply with this Code of Practice (CoP) and take action as indicated in
the quick User Guide (p. 7) depending on the nature of their work (i.e. depending on
whether their work demands CL2 or derogated CL3).
Anyone wishing to use the facility must first consult with the Facility Manager (or
responsible PI), complete a Biosafety Questionnaire (Form 1; serves to register all
projects for future reference and to guarantee appropriate training) and obtain
authorization.
Risk assessment
Principal Investigators are responsible for ensuring appropriate risk assessments are
carried out for all work with the materials involved prior to sample submission to the
Facility. These will form the basis for completing Form 1. Where the samples include
materials that have been genetically modified the GM risk assessment must be
approved by the School GM Safety Committee in advance of any work proceeding.
GENERAL ARRANGEMENTS
Access
Access to the Facility is restricted to authorised users and visitors who will be given a
key to be returned to the Facility Manager after completion of work. An authorised user
must accompany visitors at all times. First time users need approval from their
respective PI and must contact the Facility Manager before using the facility. Continued
use of the Facility is subject to approval of the Responsible PI and the Facility Manager.
The Responsible PI or Facility Manager may, without notice, stop all or any work he
considers not being performed according to this CoP, as this may present a health and
safety risk to other users.
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Booking procedures:
The Facility will essentially operate on a first come first served scheme. Bookings will
initially be recorded via email by the Facility Manager or responsible PI and displayed
on the facility website http://www.biology.ed.ac.uk/research/groups/aebischer/confocal/
for general viewing. Please, think of letting the next ones know if you run late or if you
do not need all the time that you booked. Live samples analysed at 37 °C require 3h
equilibration of the system.
Machine
Leica SP5 live
Booking system
via Email for new users
online for registered users
Information to be given
Name, phone number, time
(start/end),
nature
of
samples analysed.
Data storage / transfer:
As a multi-user facility the PC used for microscope control and image acquisition and
initial processing will not be equipped for longer term or permanent data storage. Users
are asked to export acquired data after each session via Ethernet, transportable hard disks
or other mobile data storage systems.
System hard disks will be cleared on a routine basis to prevent data overflow and hard
disk clogging.
Acknowledgement of support:
The current instrument Leica SP5 live has been bought with an equipment grant
form the BBSRC. Publications that include data acquired on this instrument
should acknowledge this support referring to the grant number BB/E012442/1 and
be made known to Prof. Keith Matthews as reference will have to be made in reporting
on the grants outcome.
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User qualification
As different users will be working with samples requiring different Containment
Levels, training will depend on the respective requirements. Please, consult the User
Guide (FlowChart, p7) for a quick reference what your needs may be. If in doubt,
please contact the Facility Manager or Responsible PI.
Note: The Standard Operating Procedures (SOP) referred to below will not cover
Cytofluorimeter settings or data recording software procedures
(proficiency in these is assumed) but are regulating sample handling within
the facility, disinfection procedures and waste disposal.
1. All new users must demonstrate familiarity with the SOP in place for working
with non-hazardous samples (SOP I; Appendix 1) to the Facility Manager.
New users must make themselves known to the Facility Manager before using
the facility. Awareness of this CoP and training shall be acknowledged by
signing the Approved User List (Appendix 2), a Biosafety Questionnaire needs
to be filed to register the project. This will qualify users to work with nonhazardous samples.
2. Users who wish to perform analyses that require working under CL2 conditions
must be trained and must demonstrate their competence and compliance with the
respective SOP in place for this type of work (SOP II; Appendix 3) to the
Facility Manager. User names will be listed on a CL2 authorized user list
(Appendix 4b). Work can only proceed if the Biosafety Questionnaire (Form
1) detailing the work is filled in by the Principal Investigator and accepted by
the Facility Manager.
3. Users who wish to perform analyses or sorting of samples that require
working under CL3 (derogated) conditions must be trained and must
demonstrate their competence and compliance with the respective SOPs in
place for this type of work (SOP III; Appendix 5) to the Facility manager.
Such users of the facility must also be an authorised user of a CL3 laboratory
having satisfactorily completed training to work unsupervised at CL3.
Records will be kept of staff training (Appendix 6a) and their name will be
listed on a CL3 authorized user list (Appendix 6b). Work can only proceed if
the Biosafety Questionnaire (Form 1) detailing the work is filled in by the
Principal Investigator and accepted by the Facility Manager who will send a
copy of the Form to the Ashworth Biological Safety Adviser.
Records of training (Appendix 4a) will be kept on file by the Facility Manager.
If at anytime anyone working in the Facility fails to comply with this Code of Practice
for any reason, or in the opinion of Responsible PI, the Facility Manager or their own
Principal Investigator is not operating in an acceptable manner, they will be suspended
as authorised user, and will need to undergo further training. During this time the
suspended person is not allowed to work in the Facility unless supervised by an
authorised user.
Out of Hours Working
Working in the Facility out of normal working hours [8am - 8pm] is subject to the
approval of the user’s Principal Investigator and the Facility Manager. Whenever
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
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possible a contact, (colleague, family or friend for example), should be told of an
expected completion time for the work, (and be updated if necessary), in order that they
can take action if the worker fails to meet or contact them at the expected time.
Consideration should be given to the School’s arrangements for lone and out of hours
working
(see
http://safety.biology.ed.ac.uk/Health&Safetydocs/Safety%20Policy%20Sections/12_Sup
ervision.doc)
Disinfection
Appropriate disinfection procedures are defined in the SOPs for work under CL1, CL2
and derogated CL3 conditions, respectively. Machines are cleaned and disinfected as
part of the standard start up and close down procedures as indicated in the User manuals.
Virkon is routinely used for disinfecting surfaces such as benches after any spillage.
Waste disposal
Non-hazardous solid waste can be disposed in the designated bins in the Facility. Nonhazardous liquid waste fluid may be poured down the sink with copious amounts of
water. Glass material (slides or cover slips) containing non-hazardous samples may be
discarded in the provided sharps bins.
All hazardous waste has to be removed and transported back to the laboratories where
the samples were prepared and where appropriate disinfection and disposal rules are in
place (see SOP II and III for further details).
Accidents
First aid:
- In the event of an accident resulting in a wound immediately but gently encourage
it to bleed, wash thoroughly with soap and water but DO NOT SCRUB, cover with
waterproof dressing.
- In the event of contamination of skin, conjunctivae or mucous membrane
immediately wash thoroughly with copious amounts of water.
As in general within the Ashworth Laboratories, all accidents, incidents and near misses
should be reported to the person responsible for the area which in this case is the Facility
Manager and to the Ashworth Superintendent who will forward a report to the
University Health and Safety Department (see accident procedures flow chart under
http://www.safety.ed.ac.uk/resources/Bio/Guidance/General/Accident%20Procedures%2
0flow%20chart.pdf). The University Occupational Health Unit should also be informed
in the event of any accident where exposure to a pathogen or infectious material may
have occurred. A full accident record should be prepared and forwarded to the Health
and Safety Department as soon as possible. The source of any contamination (specimen,
sample, material etc) should be clearly identified and retained for testing if necessary.
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Quick reference for users intending to analyse samples in the shared
Confocal Microscopy Facility (B64;Ashworth3 Laboratories)
New?
Intro and SOP I
Complete Biosafety
questionnaire
Contact:
No
Facility manager
Samples
require
CL2?
No
Yes Training II
SOP II
Complete Biosafety
questionnaire
Contact:
Record I
Acknowledgement
and approval of
CoP and SOP I
Record II
Acknowledged and
approved SOP II
competence
Work with HG 1
samples
Work with HG 2
samples
Facility manager
Samples
require
CL3?
CL
HG
CoP
SOP
Yes Training I
=
=
=
=
Yes Training III
SOP III
Complete Biosafety
questionnaire
Contact:
Facility manager
Containment level
Hazard Group
Code of practice
Standard operating procedure
Record III
Acknowledged and
approved SOP III
competence
Work with HG 3
samples
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FORM 1
Biosafety Questionnaire for
Shared Confocal Microscopy Facility Ashworth 3,
The Confocal Microscopy Facility will be operated as a multi-user facility where many different
samples from various sources that may contain known or unknown human pathogens are investigated
live. The safety of the staff and of users of the facility is of ultimate concern. Information about the
sample sources and potentially infectious agents is critical for effective biosafety measures.
Consequently, this sample information form must be filled out completely and signed by PI who is
requesting samples to be analyzed in the shared facility before experiments or projects are started. The
same biosafety questionnaire will be kept on file, the PI must inform the Facility Manager if any of the
information it contains changes. Appropriate risk assessment, and where necessary approval, of
experiments prior to sample submission to the core laboratory is required and the responsibility of the
User’s PI .
NOTE: Work performed in the facility on the Leica SP5 live microscope that is going to be
published should acknowledge the support by BBSRC Grant BB/E012442/1
Principal Investigator
Date _____________________
Researcher requesting access to facility
Name:
Phone
number
E-mail
Laboratory
PROJECT INFORMATION
Project title:
Summary or short
description of project.
Type of sample and
source
SAFETY INFORMATION
Nature of infectious agents (if known)
Hazard Group
COSHH BA1 RA Nr.
If agent is genetically modified:
Nature of GMO
Hazard Group
GM RA Nr.
BILLING INFORMATION
Institute / Group / Grant
R-Number
Cost Centre
_____________________________
Signature (Principal Investigator)
Accepted by Facility Manager:
_____________________________
Date and Signature
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APPENDIX 1
STANDARD OPERATING PROCEDURE I
RULES FOR MICROSCOPICAL ANALYSES OF Non-hazardous SAMPLES
(A copy of these rules is to be displayed in the Facility).
1. Projects need to be registered by filing the Biosafety Questionnaire (Form 1)
detailing the work is filled in by the Principal Investigator and accepted by the
Facility Manager
2. Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
outdoor clothing in the Facility is banned.
3. Mouth pipetting must not be used under any circumstances for any reason.
4. All workers in the Facility must cover cuts and abrasions with a waterproof
dressing before entering the laboratory.
5. Laboratory coats must be worn at all times whilst in the Facility.
6. Keyboards/mouse used to operate the computers must not be touched with
gloves.
7. Door handles must not be touched with gloved hands
8. Always wash hands before leaving the Facility.
9. Follow starting up and closing down procedures as indicated for the machines
used (see User manuals). In particular, the cleansing routine for the objectives
has to be followed.
10. Solid waste (TC plastic, glass slides, etc. ) may be disposed in the available bins
in the Facility or transported back to the home laboratory.
11. Any non hazardous waste fluid should be emptied down the sink with copious
amounts of water.
12. On completion of their work each user must leave the area they have used clean
and tidy. All waste and samples etc should be disposed of or removed and
surfaces and work place must be disinfected with.
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APPENDIX 2 page _____
CONFOCAL MICROSCOPY FACILITY ROOM B64 (ASHWORTH 3
BASEMENT)AUTHORISED USERS FOR WORK INVOLVING NONHAZARDOUS SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP I of the CoP to the signed
authorized user
Date
User name/Email
Signature
Authorized by Name
Signature
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APPENDIX 3
STANDARD OPERATING PROCEDURE II
RULES FOR MICROSCOPICAL ANALYSES OF LIVE SAMPLES
REQUIRING CONTAINMENT LEVEL 2 (CL2)
(A copy of these rules is to be displayed in the Facility).
1 Work can only proceed if the Biosafety Questionnaire (Form 1) detailing the
work is filled in by the Principal Investigator and accepted by the Facility
Manager.
2 Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
outdoor clothing in the Facility is banned.
3 Mouth pipetting must not be used under any circumstances for any reason.
4 All workers in the laboratory must cover cuts and abrasions with a waterproof
dressing before entering the Facility.
5 Laboratory coats must be worn at all times whilst in the Facility.
6 Door handles must not be touched with gloved hands
7 Always wash hands before leaving the Facility.
8 Samples have to be transported to and from the facility in a secondary container
with a secure lid.
9 Gloves must be worn for all manipulations of the sample Containers holding
potentially infective material. Gloves must not be worn when operating
computers or control panels. (i.e. the hand changing samples on the sample bay
is gloved; the hand operating computers is not).
10 Waste fluid if generated has to be collected in appropriate containers to be
removed from the facility after completion of the work.
11 Follow start up and closing down procedures as indicated for the machines used
(see User manuals and machine operating procedures). In particular, the
cleansing routine for the objectives has to be followed.
12 All waste material (plastic containers from the samples, glass slides, gloves worn
when manipulating samples, wipe down paper) must be RETURNED to the
home laboratory observing contained transport rules and disposed of as defined
under the local waste disposal rules.
13 On completion of their work each user must leave the area they have used clean
and tidy and disinfect surfaces where samples were handled with 1% Virkon.
14 Spills should they occur must be immediately disinfected with Virkon granules.
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APPENDIX 4a
DOCUMENTATION OF TRAINING FOR CONFOCAL MICROSCOPY
FACILITY WITH SAMPLES REQUIRING CONTAINMENT LEVEL 2
Trainee: ………………………………………….
Date of commencement of training: ……………..
Supervisor: ……………………………………….
Procedure
a
Transporting samples
c
Analyzing samples with gloves on
and operating computer
keyboard/mouse with gloves off.
Removal of contaminated waste ,
disinfection of instrument and work
place.
Safe transport and disposal of waste
at the end of the work session.
Procedures to be adopted in the event
of a spillage
d
e
f
Date demonstrated by
training supervisor
Date of satisfactory
practice
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APPENDIX 4b page________
CONFOCAL MICROSCOPY
FACILITY ROOM B64 (ASHWORTH 3
BASEMENT) - AUTHORISED USERS FOR WORK INVOLVING
CONTAINMENT LEVEL 2 SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP II of the CoP to the
signed authorized user
Date
User name/Email
Signature
Authorized by: Name
Signature
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APPENDIX 5
STANDARD OPERATING PROCEDURE III
RULES FOR ANALYSES OF LIVE SAMPLES REQUIRING CONTAINMENT
LEVEL 3 (CL3)
1
Work can only proceed if the Biosafety Questionnaire (Form 1) detailing the
work is filled in by the Principal Investigator and accepted by the Facility
Manager Work under derogated CL3 must be arranged in advance with the
Facility Manager in order that Facility can be dedicated to that use. Note that the
manager will have to give notice to other users in due time (~ 2 weeks in
advance) by email, electronic calendar and door notice.
2
The Facility must be brought up to CL3 (derogated) standards (only
together and in agreement with Facility Manager):
a. Access to the Facility must be restricted by locking the door.
b. Door signs must be fixed to indicate CL3 work (with derogations).
c. Only users trained and authorized to perform CL3 work are allowed
access and use of the Facility is limited to CL3 work for the time
required.
d. Dedicated laboratory coats must be worn at all times whilst in the Facility
and removed before leaving for any reason.
3
Eating, chewing, drinking, smoking, applying cosmetics, storing of food and
outdoor clothing in the Facility is banned.
4
Mouth pipetting must not be used under any circumstances for any reason.
5
All workers in the Facility must cover cuts and abrasions with a waterproof
dressing before entering the laboratory.
6
The use of sharps is banned in the Facility.
7
Samples have to be transported to the facility in a secondary container with a
secure lid appropriate for CL3 materials.
8
Gloves must be worn for all manipulations of the sample containers holding
infective material. Gloves must not be worn when operating computers or
control panels.
9 Door handles must not be touched with gloved hands.
10 Always wash hands before leaving the Facility
11 Waste fluid if generated has to be collected in appropriate containers to be
removed from the facility after completion of the work.
12 Follow start up and closing down procedures as indicated for the machines used
(see User manuals and machine operating procedures). In particular, the
cleansing routine for the objectives has to be followed.
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13 On completion of the work the Facility must be thoroughly cleaned and
disinfected. Surfaces and work place (including the floor), must be disinfected
with 1% Virkon (in particular area of the sampler bays).
14 All waste material from the samples (containers, gloves worn when handling,
wipe down paper) including waste fluid and the laboratory coats must be
bagged and RETURNED to the home CL3 (derogated) laboratory and disposed
of as defined under the local waste disposal rules.
15 Spills must be immediately disinfected with Virkon granules.
16 After removal of all specific waste and disinfection of all working surfaces, the
laboratory is returned to normal operating standard:
a. Door signs are removed.
b. A decommissioning checklist (Appendix 7) MUST be completed.
c. Access restrictions are brought back to normal operating level.
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APPENDIX 6a
DOCUMENTATION OF TRAINING FOR CONFOCAL MICROSCOPY
WITH SAMPLES REQUIRING CONTAINMENT LEVEL 3
Trainee: ………………………………………….
Date of commencement of training: ……………..
Supervisor: ……………………………………….
Procedure
a
b
c
d
e
f
g
Date demonstrated
by training
supervisor
Date of satisfactory
practice
Preparation of Facility for
temporary work under CL3
Transporting samples
preparing machine (preparation
and installing of dedicated waste
collectors )
Analyzing samples with gloves on
and operating computer
keyboard/mouse with gloves off.
Removal of waste , disinfection of
instrument and work place*.
:
Safe transport and disposal of
waste at the end of the work
session.
Procedures to be adopted in the
event of a spillage
This training record is solely for shared Confocal Microscopy Facility. Users of the
Facility must also be an authorised user of a CL3 laboratory having satisfactorily
completed training to work unsupervised at CL3.
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APPENDIX 6b page___________
CONFOCAL MICROSCOPY
FACILITY ROOM B64 (ASHWORTH 3
BASEMENT) - AUTHORISED USERS FOR WORK INVOLVING
CONTAINMENT LEVEL 3 SAMPLES
The Undersigned acknowledge the rules of the CoP Confocal Microscopy Facility,
Ashworth 3 and have demonstrated competence for SOP III of the CoP to the
signed authorized user
Date
User name/Email
Signature
Authorized by: Name
Signature
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LABORATORY DECOMMISSIONING CHECKLIST - CONFOCAL MICROSCOPY FACILITY ROOM B64 (ASHWORTH 3)
COMPLETED BY
Name
Date
Actions required:
APPENDIX 7
CHECKED BY
Name
Date
Check all biological materials have been removed from the lab – check benches, equipment, sink,
floors etc.
Check all lab coats have been removed.
Check all wastes have been taken away – do not sign off until everything has been removed.
Check warning labels on door have been removed.
Clean all sinks that may have been contaminated with biological materials – this includes any that have
been used whilst the lab is at CL3.
Clean all benches and surfaces that may have been contaminated with biological materials – this
includes any that have been used whilst the lab is at CL3.
Clean, and disinfect as appropriate, any items of equipment that may have been contaminated with
biological materials – this includes anything that has been used whilst the lab is at CL3.
Clean, and disinfect as appropriate, any other areas that may have been contaminated with biological
materials - this includes anything that has been used whilst the lab is at CL3 such as the floor.
Declaration:
This laboratory has been left in a condition such that it is safe for any personnel to enter without taking any precautions against exposure to
biologicalaterials.
Print name: ……………………………….….………….. Signature: …………..……………………………..……..………. Date:……………….
School: ……………………………………………………….…... Unit/Institute: ……………..………………………………………………………
LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
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LIST OF SUPPORTIVE DOCUMENTS FOR USE OF CONFOCAL MICROSCOPY FACILITY
DOCUMENT
1
2
3
4
TITLE
ON-OFF-CLEANING (QUICK GUIDE)
LAS-SOFTWARE (QUICK GUIDE )
SP5 SAFETY
SP5 FULL MANUAL
URL (TO BE COMPLETED)
LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB
20
Code of Practice Multiuser Confocal Microscopy Facility, Ashworth 3
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CHARGES FOR FACILITY USE: (STATUS 24.04.2008)
21
APPENDIX 9
General comment:
Please contact facility manager to discuss how to start your project and find out how charges apply.
CHARGES TO BE COSTED INTO GRANT APPLICATIONS:
The instrument has been funded by A REI Grant from the BBSRC (grant ref BB/E012442/1) and for this reason two different costing schemes apply:
FUNDING SOURCE
BBSRC Grants
Grants of other agencies
Rate/h (as of 24.04.2008)
£ 31.94
£ 56.33
LEAVE THIS CHECKLIST WITH THE FACILITY MANAGER. TAKE A COPY FOR YOUR RECORDS IN THE HOME CL3 LAB