Supplementary Table 1: Identification of xylooligosaccharides observed in MALDITOF-MS spectra
Name of xylooligosaccharide
Theoretical (Mw)
Xylobiose
Xylotriose
Xylotriose
Xylotetraose
Xylotetraose
Xylotriose + p-coumaric acid
Xylopentaose
Xylopentaose
β-L-4-deoxyenolpyaranosylurono-D-xylotetraose
4-O-methyl-α-D-glucorono-p-D-xylotetraoses
Xylohexaose
Xyloheptaose
4-O-methyl glucuronoxylotriose/ferulic acid +Ac
4-O-methyl glucuronoxylotriose/ferulic acid + (Ac) 3
4-O-methyl glucuronoxylopentaose
282.2
414.39
437.38
546.5
568.8
560.5
701.11
714.6
727
759
833.77
965.9
619.53
647.53
1053
Observed (Mw)
282.9
413.2
437.15
544.95
569.1
569.1
701.64
714.88
727.36
757.36
833.11
965.09
619.46
647.48
1052.63
Ten μl of each sample was loaded on TLC plates, developed in a solvent system
containing n-butanol: ethanol: water (5:3:2 v/v), air dried and sprayed with anilinediphenylamine reagent and kept at 100 oC for 1 h and observed for the appearance of
blue spots against white background. Another part of TLC corresponding to the spots
was scraped and dissolved in 200 μl of distilled water, centrifuged at 7000 xg and the
supernatant was used for the MALDI-TOF-MS analysis. The supernatant (1 μl) was
mixed with 1 μl of matrix (2, 5-dihydroxybenzoic acid, 10 mg ml-1 in 50 %
acetonitrile) and analyzed. The molecular masses were matched with the theoretical
masses of xylooligosaccharides.