User Information Plan Template
Document Information:
Filename
Current Owner
Product Identifier
Project Identifier
Current Lifecycle Phase
Revision Log:
Version
Date
A.00
December
17, 2006
Form Rev. Apr. 10, 2000
USERINFO.DOC
Deborah Perkins
R1669A Rapid Resolution Liquid Chromatography Method
Development and Optimization
CPE Release
Reason For Update
Initial
Expected Release Date: December 2006
Overview
This course is taught as a hands on method development and optimization course
for rapid resolution chromatography. This is not an introductory course and the
students should be thoroughly familiar with HPLC method development.
R1669A
Rapid Resolution Liquid Chromatography Method Development and
Optimization
R1669ainst.pdf – instructor manual pdf for viewing and printing
R1669ast.pdf– student manual pdf for viewing and printing
R1669ast.doc – student and instructor manual (turn on hidden text to view the instructor
manual, paragraph symbol in Word tool bar)
Modules
01_theory – Theoretical Fundamentals of RRLC and HPLC
02_method –Introduction to Rapid Resolution Liquid Chromatography (RRLC)
03_instrumentation – Rapid Resolution Liquid Chromatography Instrumentation
04_Laboratory Exercises - Rapid Resolution LC Method Development and Optimization
All slides are .ppt files, for instance, 01_theory.ppt.
Agilent Technologies
For Internal Use Only
Product Delivery Requirements
1.Instructor or Delivery Agent skills required to deliver the product:

Instructor must have thorough knowledge and understanding of the HPLC and RRLC.

Instructor must have an understanding of HPLC and RRLC theory and applications.

Instructor must know and understand how to use spectral data including: how to
explain and use the peak purity software; how to use and demonstrate isoabsorbance
plotting; and how to build a UV library.
2. Equipment (Instrumentation and Software) Required for product delivery
Laboratories in this course require instruments: The recommendation is an instrument for
every 4 customers.
Agilent 1200 SL Series Modules:

Micro Degasser – G1379B

Binary Pump - G1312B

Wellplate sampler – G1367C

Heated Column Oven – G1316B with/without low dispersion kit

Diode Array Detector G1315C

0.12 mm Capillary set
Current HPLC 3D ChemStation Configuration and Software:

B.02.01 SR1 G2170AA Single Instrument LC2d ChemStation Software

B.02.01 SR1 G2180AA Spectral Processing Software
Current Configuration: Windows XP (service pack 1a) and Windows 2000 (service pack 4) based
systems:
• Hewlett-Packard / Compaq PC
with Pentium III*, 600 MHz
• XGA display (1024x768
resolution)
• 8 GB hard disk
• MS Windows compatible
pointing device
• ATAPI CD, CD-RW or DVD
drive
• 10/100 baseT LAN interface
card.
Minimum Memory
Specifications:
• 256 MB RAM for 2D or 3D,
single or multiple instrument
configurations.
Agilent Technologies
For Internal Use Only

Printers

Classroom Projection System (at least 800 x 600 resolution)

Classroom Computer for slide presentation and software demonstration.
3. Consumables required

HPLC Grade Water

HPLC Grade Acetonitrile

Phenol test mix – Catalog numbers by Sigma Aldrich










0.01ug/ul Acetanilid (11,293-3)
0.013ug/ul Acetophenone (A1,070-1)
0.020ug/ul Propiophenone (P5,160-5)
0.033ug/ul Butyrophenone (12,433-8)
0.066ug/ul Benzophenone (B930-0)
0.10ug/ul Valerophenone (V659-2G)
0.20ug/ul Hexanophenone (H1,245-5) "see Pentyl-phenylketon"
0.10ug/ul Heptanophenone (159913-5G)
0.10ug/ul Octanophenone (319775-1G)
diluted in 2:1 water/ACN

Electrospray LC Demo Sample – Part No: 59987-20033

Zorbax C18 columns


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SB-C18, ID 4.6 mm, length 100 mm or 50 mm, Particle size 3.5 μm
SB-C18, ID 4.6 mm, length 100mm or 50 mm, Particle size 1.8 μm
SB-C18, ID 4.6 mm, length 100 mm, Particle size 1.8 μm
SB-C18, ID 2.1 mm, length 100 mm, Particle size 1.8 μm
SB-C18, ID 4.6 mm, length 50 mm with particle size 1.8 μm
SB-C18, ID 4.6 mm, length 50 mm or 30 mm with Particle size 1.8 μm
SB-C18, ID 2.1mm, length 30 mm with Particle size 1.8 μm
RRHT 600 bar column with 1.8 μm particle size and short column length
Flow Cells: Standard, Semi-Micro (5 µl) and Micro (2 µl)
4. Sample and ChemStation File Preparation
Lab Exercises
Dilute Phenol test mix 2:1 Water/ACN
5. Electronic Files required
None
Agilent Technologies
For Internal Use Only
6. Tools required
None
7. Activities required prior to product delivery
This course requires a student manual. There is also an instructor manual available.
Please consult the instructor’s manual for details.
1. Set-up and configure an instrument per four customers.
2. Each ChemStation must have
Windows Windows XP
B.02 01 SR1 ChemStation Software.
Configured printer with paper may be shared
CD-ROM Drive
3. Configure printers and add paper.
4. The lecture room should be equipped with a projection system for slide and software
display. A classroom computer should be loaded with ChemStation software for
classroom demonstrations.
8. Activities required during product delivery
See instructor manual for detailed directions.
Distribute and collect course evaluations at course conclusion.
9. Activities required after product delivery
Contact Deborah_perkins@agilent.com for any course problems encountered.
Agilent Technologies
For Internal Use Only