Standard Operating Procedures
Laboratory Specific
Chemical: 4,4'-Methylenebis(2-chloroaniline) (MBOCA)
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:________________________
Date when SOP was written:_______
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
4,4'-Methylenebis(2-chloroaniline) (MBOCA) is a substance used as a curing agent in
polyurethane production. It is a suspected human carcinogen.
It is a weak base with a slight odor and is reactive to active metals such as sodium, potassium,
magnesium and zinc.
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
101-14-4
Class:
Regulated Carcinogen
Molecular formula:
C13H12Cl2N2
Appearance:
off-white powder
Molar mass:
267.15 g/mol
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Density:
1.354g/cm3
Melting point:
102-107°C
Boiling point:
412°C @ 760mmHg
Solubility in water:
insoluble
Potential Hazards/Toxicity
Based on available data for 4,4'-Methylene bis (2-chloroaniline) repeated exposures may produce
cyanosis and cancer. May effect kidney function. The following have been observed in
experimental animals only: Pulmonary carcinoma, malignant liver tumors, carcinoma of the breast
and bladder and methemoglobinemia.
INHALATION: May produce cyanosis. 4,4'-Methylenebis-2-chloroaniline may cause cancer based
on tests in laboratory animals. May cause respiratory sensitization in susceptible individuals. At
room temperature, vapors are minimal due to low vapor pressure. If heated, excessive
concentrations are attainable that could be hazardous on single exposure.
EYE CONTACT: Vapors from heated product may cause eye irritation and severe damage.
SKIN CONTACT: Hazardous components are absorbed through the skin. 4,4'-Methylenebis-2chloroaniline may cause cancer based on tests in laboratory animals. May produce cyanosis.
May cause allergic skin reaction in susceptible individuals. Prolonged or repeated exposure may
cause skin irritation.
INGESTION: Material may be ingested through poor hygiene practices. May cause
gastrointestinal, kidney, and central nervous system damage. May produce cyanosis. 4,4'Methylene bis-2-chloroaniline single oral dose toxicity: Oral LD50 for rats is 2,100 mg/kg.
TARGET ORGANS: May effect kidney function.
CHRONIC: Based on available data for 4,4'-Methylene bis (2-chloroaniline) repeated exposures
may produce cyanosis and cancer. The following have been observed in experimental animals
only: Pulmonary carcinoma, malignant liver tumors, carcinoma of the breast and bladder and
methemoglobinemia.
Monitoring
Special urinalysis should be conducted to determine that no employee's urine contains >100 mg
of MBOCA/liter of urine when the specific gravity (SG) is adjusted to 1.024. When urine sample
are not received, the exposure shall not exceed an 8-hour time weight average (TWA)
concentration of MBOCA in excess of 10 r/m3 of air and employee shall not be exposed to a
ceiling concentration in excess of 50 rg/m3 during any 15 minute period. Accessible surfaces
which employees are likely to contact in controlled access areas during their normal work shall
not exceed 100 rg MBOCA/100 cm2 of surface. The average of at least five wipe samples shall
be used.
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If one sample exceeds the mean by a factor of 10 or more, it will be rejected as not being
representative. Use of >1 kg of MBOCA, irrespective of concentration, dilution, or form, except
frozen pre-mix, in any 6-month period, a program of surface-contamination monitoring and
urinalysis (for MBOCA) should be instituted. Any employee entering a controlled-access area will
have the urinalysis repeated quarterly with samples to be collected near the end of the work shift.
Any employee whose urinary MBOCA level exceeds 100 rg/1 with SG adjusted 10 1.024, shall be
tested at least each month until two consecutive samples taken not <3 days apart have been
reduced to <100 rg/1. The method of monitoring and measurement shall be accurate and precise
so that 95% of the determination are within 25% of the true value at the exposed limit. Employees
are to be notified in writing, if exposed in excess of PEL, within 10 days of the numerical results of
the monitoring.
Personal Protective Equipment (PPE)


All persons shall wear personal protective equipment when handling 4,4'-Methylenebis(2chloroaniline). This includes wearing a lab coat, nitrile gloves, closed toe shoes, and
chemical splash goggles when working with 4,4'-Methylenebis(2-chloroaniline). Gloves
should be changed frequently.
Leave lab coats, gloves, and other personal protective equipment in the lab once your
work is complete to prevent the spread of this or other chemicals outside of the lab.
Engineering Controls
Always handle 4,4'-Methylenebis(2-chloroaniline) in a certified chemical fume hood. Use dry
materials in a fume hood, or choose premixed solutions to avoid inhalation exposure.
First Aid Procedures
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Inhalation or Ingestion: In the case of 4,4'-Methylenebis(2-chloroaniline) ingestion, obtain
medical attention immediately.
Eyes exposure: Immediately flush eyes with copious amounts of water for at least 15
minutes, preferably in an emergency eyewash.
Skin exposures: In the event of skin exposure, remove contaminated clothing and
immediately wash the affected area with soap and copious amounts of water for 15
minutes.
Special Handling and Storage Requirements
1. Signage is required for the area where 4,4'-Methylenebis(2-chloroaniline) is stored.
Signage should read “Controlled Access Area Authorized Personnel Only”.
1. All work with 4,4'-Methylenebis(2-chloroaniline) is to be done in an " 4,4'Methylenebis(2-chloroaniline)" designated area in order to keep 4,4'-Methylenebis(2chloroaniline) contamination to a minimum. Any persons in this area are required to wear
personal protective equipment. Safety shower and eye wash stations should be easily
accessible where 4,4'-Methylenebis(2-chloroaniline) is used.
2. All laboratory equipment (such as beakers, pipettes, etc.) used in the "4,4'Methylenebis(2-chloroaniline)" designated area are to be labeled as
“CAUTION 4,4'-Methylenebis(2-chloroaniline). Avoid skin contact. Avoid breathing
dust/vapor of this regulated carcinogen”.
3. Wash thoroughly after handling. Remove contaminated clothing and wash before
reuse. Use with adequate ventilation (fume hood). Minimize dust generation and
accumulation. Do not get in eyes, on skin, or on clothing.
4. Keep container tightly closed. Avoid breathing dust.
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5. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from
incompatible substances. Containers should have a “cancer hazard” label and be placed
in a designated area with secondary containers labeled “cancer hazard”.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors.
Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small
area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g.,
use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
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Decontamination/Waste Disposal Procedure
Waste disposal procedures
1. All 4,4'-Methylenebis(2-chloroaniline) contaminated waste shall be disposed of into waste
containers specifically designated for 4,4'-Methylenebis(2-chloroaniline) waste.
2. No waste streams containing 4,4'-Methylenebis(2-chloroaniline) shall be disposed of in
sinks.
3. Wash hands and arms with soap and water after finished.
4. Once the waste container is full, dispose of according to the UCLA EH&S hazardous
waste guidelines.
Waste containers containing 4,4'-Methylenebis(2-chloroaniline) (a Particularly Hazardous
Substance) must be labeled:
“DANGER, CANCER HAZARD”
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)

Prior to conducting any work with 4,4'-Methylenebis(2-chloroaniline), designated
personnel must provide training to his/her laboratory personnel specific to the hazards
involved in working with this substance, work area decontamination, and emergency
procedures.

The Principal Investigator must provide his/her laboratory personnel with a copy of this
SOP and a copy of the 4,4'-Methylenebis(2-chloroaniline)’s MSDS provided by the
manufacturer.

The Principal Investigator must ensure that his/her laboratory personnel have attended
appropriate laboratory safety training or refresher training within the last two years.
I have read and understand the content of this SOP:
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Date
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