B.S. Cheng and K.A. Hovel Authors: Brian S. Cheng and Kevin A. Hovel Manuscript Title: Biotic resistance to invasion along an estuarine gradient Contact Information: Brian Cheng, bscheng@ucdavis.edu, 925-876-7988 Supplementary Material 1. Proportional chalk block loss (mean ± SE) across the estuarine gradient. Sites are listed from left to right with increasing distance away from bay mouth for riprap (R) and soft-sediment (S) sites. Page 1 B.S. Cheng and K.A. Hovel Supplementary Material 2. Scatterplots of eelgrass habitat principle components (X and Y axis) against mussel counts (color) in a) 2006 and b) 2007. Interpretation can be made by comparing changes in color along each X and Y axis (for main effects). Interactive effects are evident if changes in color proceed along the diagonal. Color scale is the same for both years. Page 2 B.S. Cheng and K.A. Hovel Supplementary Material 3. Pooled Asian mussel recruitment at R1 and R2 sites in 2007 (mean mussel counts per core ± SE, left axis) and shell height (mean ± SE, right axis). Page 3 B.S. Cheng and K.A. Hovel Supplementary Material 4. Length frequency histograms of all Asian mussel recruits collected at R1 and R2 during the recruitment pulse in 2007. Page 4 B.S. Cheng and K.A. Hovel Supplementary Material 5. Results from fully caged artificial seagrass units deployed along the estuarine gradient. Proportional mussel survival (mean + SE) in a) 2006 (n = 22) and b) 2007 (n = 12). c) Dry weight (mean + SE) in 2007 and d) gonad index (mean + SE). Gonad index is calculated as gonad weight divided by total body weight. Letters indicate group designations from Student-Newman-Keuls post-hoc tests in C & D (P < 0.05). ND = no data, data excluded due to predator intrusion into plots. Sites are listed from left to right by increasing distance away from bay mouth. Page 5 B.S. Cheng and K.A. Hovel Supplementary Material 6. Eelgrass habitat structure along the estuarine gradient. Shoot density (mean number per core +SE, closed circles) and shoot length (mean +SE, open circles) in a) 2006 and b) 2007. Page 6 B.S. Cheng and K.A. Hovel Supplementary Material 7. ANOVA table for the restricted analysis on open plots at sites less than 3.5 km from the bay mouth testing the effects of distance and habitat and their interaction on proportional mussel mortality. Adjusted r2 = 58.2%. Source SS df MS F-ratio P Distance 3.812 1 3.812 50.440 <0.001 Habitat 0.716 1 0.716 9.48 0.003 Distance * Habitat 1.054 1 1.054 13.953 <0.001 Error 3.930 52 0.076 Page 7 B.S. Cheng and K.A. Hovel Supplementary Material 8. Proportional Asian mussel mortality (mean + SE) in all cage treatments after seven day deployments across the estuarine gradient. Data from years 2006 and 2007 have been pooled for brevity. Page 8