B7 Biology across the ecosystem • advance planning page 1
TWENTY FIRST CENTURY SCIENCE
B7 Biology across the ecosystem
Technicians’ advance planning sheets
This table shows items that may need to be ordered in advance for this module. It
does not list standard items, for example, test tubes, OHT pens etc.
Full details of the resources needed for each activity are given in the Requirements
section of the Teacher & Technician notes, which also include safety information for
the activity.
Activity Item(s)
Quantity per
student / group
of students, or
for the teacher
Notes
AB7.1
Per group
Elodea is used by the leaf
rather than the whole plant
so less is needed
Per group
Geranium needs to be
destarched for at least 24
hours before experiment
Elodea
Microscope with a 40x
objective
AB7.2
Geranium
Potassium hydroxide
Ethanol
Iodine solution
Limewater
AB7.3
Molymod models
Per group
Sufficient models to make
6 carbon dioxide and
6 water molecules
AB7.4
Young nettle or spinach
leaves
Per group
The petroleum ether and
the acetone are to
produce a
chromatography solvent.
The lower boiling point
mixtures give better
results but are more
flammable
Per group
Visking tubing needs to
soaked at least an hour
before use
Per group
Cabomba which can be
obtained from aquarium
shops works as well if not
better than Elodea
Chromatography paper
Hairdryer (optional)
Petroleum ether (all
boiling points)
Acetone (propanone)
AB7.7
Visking tubing
Capillary tubing
Starch solution
Glucose solution
AB7.8
Elodea
Sodium hydrogen
carbonate
Lamp
Light meter (optional)
Audus micropipettes can
be used in this experiment
B7 Biology across the ecosystem • advance planning page 2
AB7.9
Datalogging software &
relevant computer
equipment
Per class
You need at least 6-12
sprigs of Elodea
Light sensor
Oxygen sensor
pH sensor (optional)
Temperature sensor
(optional)
Magnetic stirrer
Elodea
Sodium hydrogen
carbonate
IB7.4
Computer access with
Excel
Per group
AB7.12
Tin lid
Per group
Gas jar and lid
Damp soil
Digital balance
(1 decimal place is
acceptable)
AB7.18
Yeast suspension
Per group
Sucrose
Lactose
Fructose
1g of Yeast nutrient can
be replace by 0.5g of
ammonium dihydrogen
orthophosphate and 0.5g
of ammonium sulphate
Dataloggers, magnetic
stirrers and bubble
counters may be used
Yeast nutrient
Phenolphthalein solid
Ethanol
Sodium hydroxide
Liquid paraffin
Alkaline phenolphthalein
solution
AB7.19
Bacterial transformation
kit
Access to incubator
Small polystyrene
container of crushed ice
If pGLO kit used, access
to long wave UV light
Per group
The Bacterial
transformation kits are
available from Bio-Rad or
the NCBE. Details are in
the C21 Teacher &
Technician guide for B7.
B7 Biology across the ecosystem • advance planning page 3
AB7.21
Lambda DNA protocol or
DNA Fingerprinting Kit
Per group
NCBE (lambda DNA)
Gel electrophoresis tanks
Bio-Rad (DNA
fingerprinting)
Micropipettes
Other laboratory
materials as required by
kits
AB7.27
AB7.29
The kits are available from
Economatics (DNA
fingerprinting)
Some kits contain gel
electrophoresis tanks and
micropipettes
Metronome
Per class
Access to stairs or 30cm
high bench
Per pair/group
ATP solution in a 1cm
syringe
Per class
1% glucose in 1cm
syringe
Meat needs to be very
fresh and cut parallel to
the fibres, so that each
group has a 1cm wide by
3cm long strip
Distilled water in a 1cm
syringe
Strips of fresh pork or
beef
Per group
Dissecting needle
AB7.30
Chicken wings
Disposable plates
Per group
Raw chicken should be
used
Dissecting scissors
Blunt probe
© in this format University of York (UYSEG) and Nuffield Foundation 2007