UQBR
Registration Request Form – Embryo cryopreservation (Internal)
Date:
____________________________________________________________________________________
Contact Details
Chief Investigator
Name:
Email Address:
Contact Phone:
Department/Institute:
Contact Researcher/Alternative Contact
Name:
Email Address:
Department/Institute:
Fax:
Contact Phone:
After Hours Phone Contact:
Billing/Mailing Address:
Dept/Building/Street Address:
____________________________________________________________________________________
Animal Ethics Approval Details (Please attach a copy of the AEC Approval Certificate)
AEC Approval No.:
Expiry Date:
Project Title:
IBC/OGTR No:
Dealing Type (eg DNIR, NLRD):
_____________________________________________________________________________________________
Service Required: Embryo Freezing of Mouse Lines
Mice Details:
Number of strains: ...........................................................................................................................
Name of strain/s: ............................................................................................................................
Genotrack Colony ID (if applicable): …………….……………………………………..…………………………………….
Sex of mice supplied: male / female / both
Genotype of mice supplied: WT / heterozygous / homozygous
Strain of wild type mouse for mating (if applicable): ....................................................................
DECLARATION OF UNDERSTANDING AND COMPLIANCE
I, the undersigned agree to and have read the following items of information:

Heterozygous/Homozygous females can be supplied by Client at the appropriate age for strain. Age
of females is preferably 8-16 weeks. Wild type females can be supplied by TASQ at an additional
cost.

Males used as studs must be of proven fertility. If no proven males are available, TASQ can test
fertility at additional cost to the researcher.

As there are strain variations in response to superovulation, more than 20 females may be
necessary to produce the required embryo numbers for storage. Therefore more than 20 females
may be needed to cryopreserve the line. Additional charges will apply per 20 females used for
embryo freezing.

TASQ stores embryos in 2 separate tanks. Refer to the TASQ website for details regarding the
number of embryos to be stored http://tasq.uq.edu.au/embryo-cryopreservation.

TASQ will supply a progress report on each strain’s success regarding the number of embryos
collected after the first round of cryopreservation. Once the required numbers are reached, TASQ
will notify client and await instructions on the fate of the mice held at TASQ.

Health report must be sent to UQBR prior to the shipping of mice.

Client is responsible for shipment costs to TASQ.

Client has obtained an Animal Ethics approval and expiry date. Certificate must be attached to this
registration.

Have obtained OGTR approval and quoted this number.

A test thaw and rederive is conducted to test the viability of the strain for freezing. The thaw
results will be relayed to the client.

When UQBR covers the costs of cryopreservation, there is a cost of $260/strain to the researcher
for the test thaw. This involves the thaw and culture of the embryos and then embryo transfer into
a pseudopregnant mother. The thaw results will be relayed to the client. This fee is compulsory.

The generation of this genetically modified mouse strain will not infringe any intellectual property
rights.

I have checked the TASQ website at http://tasq.uq.edu.au and agree to the charges listed for this
service.
Signature of investigator: ................................................................ Date: .............................
Signature of TASQ Director: ............................................................. Date: .............................
AIBN Building 75, University of Queensland, St Lucia, QLD 4072, Australia
Tel: +61 7 3346 3468 Fax: +61 7 3346 3898
email: Elizabeth.Williams@uq.edu.au or t.doan1@uq.edu.au