FermentOgram Editorial Board Thomas Jeffries, Jeff Cary, Hans Blaschek, and Joy Doran Peterson Spring, 2007 NOTES FROM DIVISION O CHAIR sources of energy. Colloquium participants made a number of recommendations for moving forward with research and education in this important field. One of the recommendations was to have interdisciplinary cooperation and work in teams to carry out multidisciplinary research. The recent interest in microbial energy technologies bodes well for our Division. There has been considerable recent interest in the renewable production of energy. While we have known for some time the role that microbes play in various fermentation processes for producing ethanol and other liquid fuels, the application of the new tools of systems biology offers us an exciting opportunity to produce feedstocks and microbes in a more concerted and tailored fashion. The study of microbial-based fuel production is by its nature multidisciplinary in nature, involving microbiologists, plant scientists, engineers, chemists, plant breeders, economists and bioinformaticists. This integrated multi-disciplinary approach to solving the problems associated with biomass conversion will involve the skills of many different experts, working together to overcome bottlenecks in the continuum. The conversion of lignocellulosic biomass to a carbon neutral liquid fuel has been described in the same vein as the "Apollo" moon program. I think you'll agree with me, these are interesting times. Hans Blaschek, Chair, Division O, ASM THE DIVISION O BUSINESS MEETING Please plan to attend the Division O Business meeting to be held after the Division O Symposium, Wednesday 5/23 at 10:30. Lunch will be provided, so please plan to attend and bring your ideas for incoming officers and symposia for next year. Thanks! If you have a moment, please email jpeterso@uga.edu if you know you plan to attend. The current day ethanol production facility is already being modified into a biorefinery where renewable commodites and higher value bioproducts will be produced and converted into polymers and other products. The new tools of genomics will allow for a focus on the development of tailor made feedstocks that can be used by tailor made fermentation microbes. ASM recently released a report on microbial energy conversion. For more information, please see: report on microbial energy conversion. This report is based on a March 2006 American Academy of THE DIVISION O MIXER Wednesday, May 23, 2007 7:00 pm at the Westin Hotel Look for room location to be posted at a later date. ABSTRACT REJECTION RATE There was a general discussion about the rejection rates of abstracts submitted to Division O. At the Annual Training Program for Officers, ASM program directors posed the question—is the society accepting too many abstracts for the ASM General Meeting? They raised the issue that a higher rejection rate may increase the quality of Microbiology colloquium in San Francisco. The report addresses the need for clean, renewable -1- our abstracts and thus possibly, lead to better attendance at our meetings. The rejection rate across the divisions ranges from 0 to 40%. As you know, the chair and chair elect read all of the submitted abstracts and decide on their acceptability. Our rejection rate averages 10%. In general, we accepted most abstracts that were not promotional in nature. Further, it was pointed out that one purpose of abstracts is to allow students to present their data in front of their colleagues and to gain from the constructive criticism of their research. The consensus was that we should not increase our rejection rate. Certainly, we would like comments from the broader membership. Nominations for 2008-2010 Group Representative We need to elect a Divisional Group Representative to serve a two year term (July 1, 2008 to June 30, 2010). If you have suggestions, please let Hans Blaschek know at blaschek@uiuc.edu. Thank you. DIVISION O STUDENT TRAVEL AWARDS CONGRATULATIONS! PROPOSED SYPOSIA FOR 2008 Please let Tom Jeffries or Joy Peterson know if you have suggestions for next year’s symposia. The ASM planning committee usually requests these symposia titles in very early June of the year prior to the meeting. This is not a final list, but rather a chance to get symposia on the program list. H.B.M. BAKBT PROPOSED SYMPOSIA FOR 2006 Convener Topic Joy Doran Peterson Microbial Biorefineries Mike Cotta S. MORRIS E. HENRIKSEN S.R. SHILPA P. ROYCHOUDHURY A. DE ALMEIDA Univ. Maryland, Eastern Shore, Princess Anne, MD U. of Strathclyde, Glasgow, UK Universidad de Buenos Aires, Buenos Aires, Argentina Rowan Univ. ,Glassboro, NJ Univ. of Georgia, Athens, GA Univ. of Madras, Chennai, INDIA STUDENT TRAVEL GRANT APPLICATIONS Division O usually has about 100 abstracts submitted for consideration for poster presentations at the ASM General Meeting. If a student will be presenting the poster there is an option that can be checked during the application process that requests the student to be considered for an ASM Corporate Sponsor Student Travel Grant. If you want your student to be eligible for one of these travel grants there are seven criteria that must be met. These criteria are as follows: (1) Student has to be the presenter; (2) Student must indicate desire to be considered; (3) Student must be an ASM member (confirmed from membership records); (4) Letter of recommendation from the department must be on file; (5) Student was not selected for a grant in the previous year; (6) Student must be in a recognized educational program. Post-Docs within one year of receiving their terminal degree are also eligible; and (7) Students in the Undergraduate Fellowship or Minority Undergraduate Fellowship Programs (managed by the Education Department) are not eligible. If any of these criteria are not met the student is not eligible for the grant. These grants are allocated to each ASM division based on the number of qualifying applicants. This year Division O was allocated six of these grants. The more abstracts that are submitted, the more chances we have for additional travel awards, provided the applicants have followed MEMBERSHIP STATISTICS Year 2003 2004 2005 2006 2007 MEMBERS Primary 899 879 891 829 807 Secondary 490 498 565 435 413 Abstracts 135 120 77 91 90 NUMBER OF ABSTRACTS PRESENTED YEAR ALL DIV. DIV. O 2002 2839 95 2003 2004 3628 3657 135 120 2005 2006 2007 2916 3001 3500 77 91 90 -2- all the directions above. Thanks, Jeff Carey, FermentOgram Editorial Board ALTERNATE COUNCILOR: Jeffrey W. Cary USDA, ARS, Southern Regional Research Center New Orleans, LA jcary@srrc.ars.usda.gov UPDATED DIVISION O WEBSITE The updated Division O website is now ready! Joy Doran Peterson, has agreed to oversee the website. The site can be accessed through the American Society for Microbiology main webpage (www.asmusa.org) under “Scientific Divisions” or directly at http://www.asmusa.org/division/o/index.html Please take a look at the new website and send comments, suggestions, items for inclusion, etc. to Joy at jpeterso@uga.edu. Funds are solicited to maintain and constantly upgrade the website. Please send your monetary support to the undersigned. Recognition of support will be given to each and every contributor. If you wish, we will also provide a link to your website accessed via your logo. The site provides information such as Division Officers, a history of Division O by Arnie Demain, membership profile, news of upcoming meetings and events, the FermentOgram, links of interest, and much more. We think you will find the Division O website quite informative and a useful tool for outreach to potential new members. This site will only be as good as the Division O membership makes it, so please be sure to visit the site and give me or any of the Newsletter editorial board members your feedback. Thanks, DIVISION O MEETING DETAILS TOPICS AND SESSIONS OF INTEREST THE 2005 DIVISIONAL LECTURE Dr. Thomas Jeffries of the Institute for Microbial and Biochemical Technology in Madison Wisconsin will deliver our Division O Lecture. Tom is internationally recognized for his work with Pichia fermentations. He will discuss “Genomics to Improve Xylose Fermentation in Pichia stipitis” in the session 21st Century Microbial Tools for the Production of Chemicals. 2007 DIVISION O SYMPOSIUM Symposium 122/O Wednesday, 5/23/2007 718A- South Bld Joy Doran Peterson, FermentOgram Editorial Board 21st Century Microbial Tools for the Production of Chemicals DIVISION O FERMENTATION AND BIOTECHNOLOGY OFFICERS CHAIR: CHAIR-ELECT: COUNCILOR: 8:00-10:30 am Conveners: S. Sariaslani and Al Laskin Division O Lecture: Functional Genomics to Improve Xylose Fermentation in Pichia stipitis T. W. Jeffries; University of Wisconsin, Madison, WI. HANS BLASCHEK, Ph.D. Professor, Food Science University of Illinois blaschek@uiuc.edu Yeast as a Versatile Chemical Factory J. Nielsen; Technical University of Denmark, Lyngby, DENMARK. Thomas W. Jeffries U.S.Department of Agriculture Madison, WI twjeffri@wisc.edu Programmable Molecular Sensors as General Tools for Optimizing Flux Through Synthetic Metabolic Networks C. D. Smolke; Cal Tech, Pasadena, CA. Joy B. Doran Peterson, Ph.D. Department of Microbiology University of Georgia, Athens jpeterso@uga.edu Advances in Acetone, Butanol and Ethanol Fermentation H. P. Blaschek; Univ. of Illinois, Urbana, -3- DIVISION O MEETING DETAILS IL. 10:00 am Renewable Resources for Production of Aromatic Chemicals S. Sariaslani; Dupont, Wilmington, DE. SPECIAL THANKS! The Editorial Board is grateful to Peter Baum, We are indebted to the following companies which, in 2006-2007, have financially (ASM) for helping us with programming details (thanks for your patience!). supported the Division O activities: NEW BRUNSWICK SCIENTIFIC CO.ABS Scientific Careers with Today’s FBI TOPICS AND SESSIONS OF INTEREST B. Budowle Session 325 Friday 5/25/2007 8:00-10:30 am 717B- South Bld. Colloquium IV: Bacterial Metabolism – Better Living Through Chemistry Colloquium: 095. Developments in the Conversion of Complex Polysaccharides into Renewable Energy Colloquium IV: Bacterial Metabolism – Better Living Through Chemistry Probing Metabolic Complexity with Experimental and Computational Approaches Tuesday, 5/22/2007 2:30:00 PM - 5/22/2007 5:00:00 PM Conveners The focus will be on the combination of computation and experimental approaches used concurrently to decipher metabolism on a global scale. Conveners R. Weiner; NSF and R. A. LaRossa; Dupont Invited Speaker Conveners: V. A. de Crecy-Lagard and A. Osterman Rational Design of Cellulases to Increase Their Activity on Crystalline Cellulose D. B. Wilson; Cornell Experimental Determination and Computational Prediction of Intracellular Fluxes Uwe Sauer, idgenoessiche Technische Hochschule Hönggerberg, Zürich, SWITZERLAND. The Ins and Outs of Designer Cellulosomes E. A. Bayer; The Weizmann Institute of Science, Rehovot, ISRAEL. Integrated Analysis of Yeast Metabolism Jens Nielsen, Technical University of Denmark, Lyngby, DENMARK Plant Cell Wall Deconstruction by the Marine Bacterium, Saccharophagus degradans S. W. Hutcheson; University of Maryland, College Park, MD. Amt proteins: Biological Gas Channels for NH3 S. Kustu, Univ. California, Berkeley, CA From tRNA Modification to Metal Chaperones: Following the Genome Path V. deCrecy-Lagard, Univ. Florida, Gainesville, FL Turning Plant Cell Walls into Sugars with Clostridium cellulovorans Cellulosomes R. H. Doi; UC, Davis, CA. Bioprocessing Cellulosic Biomass L. R. Lynd; Dartmouth College, Hanover, NH. Integrative Approach to the Analysis of Metabolic Systems and Pathways A. Osterman, Burnham Institute, La Jolla, CA Session 105 Career Development Forum: Microbiologists Make a Difference in Industry, Technology Transfer, and Law Enforcement Tuesday 5/22/2007 2:30-5:00 pm theatre- North Bld. 2007 DIVISION O POSTER SESSIONS Poster: 047/O. Antibiotics, Antimicrobials and Mycotoxins Convener: Arturo Casadevall Description:O Fermentation & Biotechnology Technology Transfer-Bridging the gapfrom the lab bench to the business community M. Melnicoff Time:5/22/2007 9:00:00 AM - 5/22/2007 12:00:00 PM Microbiology and Industry J. Steenbergen -4- Abstract and poster board number 463. Nisin Purification Using a Two-Phase Aqueous Micellar Systems P. G. Mazzola, A. F. Jozala, A. M. Lopes, L. M. Sato, S. G. Liu, T. C. V. Penna, A. Pessoa Jr.; University of São Paulo, School of Pharmaceutical Sciences, São Paulo, BRAZIL. 464. Production of Different Structural Analogs of Sophorolipids by Modifying the Composition of Fermentation Medium And Measurement of Its Antimicrobial Activity(Ctrl #: 1419) D. J. Badia1, V. Shah1, P. Ratsep2; 1 Dowling College, Oakdale, NY, 2Shimadzu Scientific, New Brunswick, NJ. 472. Absence of Growth of Klebsiella pneumonia and Pseudomonas aeruginosa Cultures Following Challenge with Purified Bacillus sp. Fermented Extract R. Michelin1, L. Frederick2, K. Lobban3, C. Johnson1, S. Pramanik1, R. Shaw1, J. Whittaker1, W. Leitner4, A. Williams1; 1Morgan State University, Baltimore, MD, 2Howard University, Washington, DC, 3University of the West Indies, Kingston, JAMAICA, 4NCI/NIH, Bethesda, MD. 473. Monensin Inhibits Growth of Bacterial Contaminants from Fuel Ethanol Plants K. M. Bischoff, S. Liu, B. S. Dien; USDA-ARS-NCAUR, Peoria, IL. 474. Inhibitory Effect of 7-O-butyl Naringenin on Growth of Staphylococcus aureus ATCC 35556 with the Microplate Culture Methods S. H. Moon, E. J. Yeo, K. T. Kim, Y. S. Park, Y. Han, S. Y. Nah, H. D. Paik; Konkuk University, Seoul, REPUBLIC OF KOREA. 465.Characterization of the Production of Reuterin, an Antimicrobial Compound Secreted by Strains of Probiotic Lactobacillus reuteri(Ctrl #: 1479) K. Hermans, L. Schaefer, R. Britton; Michigan State University, East Lansing, MI. 467.Isolation and Partial Characterization of Bacteriocin-Like Compounds from Lactobacillus rhamnosus [LCS-742] H. B. M. Bakht1, M. Harel2, A. K. Nyame1, E. B. May1, D. W. Boucaud1; 1University of Maryland Eastern Shore, Princess Anne, MD, 2Advanced BioNutrition, Columbia, MD. 475.Isolation and Selection of Thermotolerant Streptomyces Strains with High Extracellular Production of Beta-1,3Glucanase C. N. Guillermo, T. E. Sergio Ruben; Cento De Investigacion En Biotecnologia Aplicada Del Instituto Politecnico Nacional, Tepetitla de Lardizabal, TLAXCALA, MEXICO. Poster: 048/O. Fermentations and Bioconversions Description:O Fermentation & Biotechnology 468. Mechanism of Valanimycin Biosynthesis in Streptomyces viridificiens R. Garg, R. Parry; Rice University, Houston, TX. Time:5/22/2007 9:00:00 AM - 5/22/2007 12:00:00 PM 476. Physiological Approaches to Increase Molar H2 Yield in Anaerobic Fermentative Bacterial Cultures: Use of Extracellular Electron Shuttling Compounds J. L. Hatch, K. T. Finneran; University of Illinois, Champaign, IL. 469.Evaluation of Culture Medium Formulations for the Production of Geldanamycin by the Biocontrol Agent Streptomyces violaceusniger YCED-9(Ctrl #: 1791) J. C. Cercado-Jaramillo, A. Guzmán-Alonso, S. R. TrejoEstrada; CIBA-IPN, Tlaxcala, MEXICO. 470.Amazing Red Bacterium from Philippine Mangrove with Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus MRSA), Candida albicans, Bacillus cereus and Vibrio spp. R. G. Monsalud1, E. B. Leyeza2, J. D. L. S. Clavillas1, I. A. Papa1, T. O. Zulaybar1, A. R. Creencia1, N. H. T. Gana1, V. W. Bautista3; 1National Institute of Molecular Biology & Biotechnology (BIOTECH), College, Laguna, PHILIPPINES, 2Institute of Biological Sciences, University of the Philippines Los Baños, College, Laguna, PHILIPPINES, 3IAM Bioresource Center, Institute of Molecular & Cellular Biosciences, The University of Tokyo, Tokyo, JAPAN. 471.Cloning and Characterization of the Aspergillus ochraceoroseus Aflatoxin Biosynthetic Gene Cluster J. W. Cary, P. Y. Harris-Coward, K. C. Ehrlich, S. M. Beltz, M. A. Klich; USDA/ARS/SRRC, New Orleans, LA. -5- 477. Analysis of Intermolecular and Intramolecular Transglycosylation Activities of Thermus brockianus 4-αGlucanotransferase J-H. Jung1, S-W. Woo1, M-Y. Baik1, S-H. Yoo2, C-S. Park1; 1Kyung Hee University, Yongin, REPUBLIC OF KOREA, 2Sejong University, Seoul, REPUBLIC OF KOREA. 480. Modifications of Carbon Metabolism in Sugar Fermentation by Thermoanaerobacter ethanolicus 39E P. M. Lokken1, J. Zhou2, Q. He1; 1Temple University, Philadelphia, PA, 2University of Oklahoma, Norman, OK. 481. Analysis of E. Coli PHA-Producing Strains Expressing a Phasin Protein from Azotobacter sp. in Bioreactor Cultures A. de Almeida1, P. I. Nikel1,2, B. S. Méndez1, M. J. Pettinari1; 1Universidad de Buenos Aires, Buenos Aires, ARGENTINA, 2Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Buenos Aires, ARGENTINA. 483. Medium Optimization for the Antioxidant Production from Bacillus polyfermenticus SCD Using Response Surface Methodology H-D. Paik, J-H. Lee, M-S. Chae, G-H. Choi; Konkuk University, Seoul, REPUBLIC OF KOREA. 494. Laccase Production in Media Supplemented with Different Glucose Concentration by Two Trametes spp. G. Rojas-Verde, E. Alemán-Huerta, I. Quintero-Zapata, L. Galán-Wong, K. Arévalo-Niño; Instituto de Biotecnologia, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, N. L., MEXICO. 484. Mixed Alcoholic and Malolactic Fermentations in the Vinification of Cool Climate Chardonnay W. Pan1, A. Dubé Morneau2,1, R. Mira de Orduña3,1; 1Univ. of Guelph, Guelph, ON, CANADA, 2Washington State Univ., Pullman, WA, 3 Cornell Univ., Geneva, NY. 495.Optimized Production, Extraction and Purification of Xylose Isomerase from Bacillus thuringiensis tolworthi as a Newly Identified Producer J. Vaez1, R. Saghiri1, M. Tabatabaei Yazdi2, N. Khodaibooran3, A. Khadem1, H. Nemati1; 1Pasteur Institute of Iran, Tehran, IRAN (ISLAMIC REPUBLIC OF), 2Tehran - University of Medical Sciences, Tehran, IRAN (ISLAMIC REPUBLIC OF), 3Sick Children Hospital- The University of Toronto, Toronto, ON, CANADA. 485. Electricity Generation from Carbon Monoxide Containing Syn-Gas: CO Fermentation Combined with Microbial Fuel Cell Technology D. Kim, I. Chang; Gwangju Institute of Science and Technology (GIST), Gwangju, REPUBLIC OF KOREA. 486. Purification of a Herbicidal Compound from Streptomyces sp 1M5a A. Guzmán-Alonso, J. C. CercadoJaramillo, S. R. Trejo-Estrada; CIBA-IPN, Tlaxcala, MEXICO. 496. Improved Bioethanol Productivity Using E. coli FBR5 Derivitives with Dual Resistance to Furfural and Ethanol S. H. Morris, V. M. Foring, K. A. Riegel, B. G. Lefevbre, M. J. Savelski, G. B. Hecht; Rowan University, Glassboro, NJ. 487. Investigation of Lignocellulose Degrading Enzymes from Paenibacillus amylolyticus TA64, Isolated from the Hindgut of Tipula abdominalis E. D. Henriksen, J. B. D. Peterson; University of Georgia, Athens, GA. 497. Engineering Escherichia coli B for Production of Succinate in Minimal Salt Medium K. Jantama, M. J. Haupt, X. Zhang, J. C. Moore, L. O. Ingram; University of Florida, Gainesville, FL. 488. Investigation of the Nutritional Requirements of Wine Lactic Acid Bacteria by Cultivation Methods and Genetic Algorithms N. Terrade1, M-A. Bondeau2,1, M. Doherty1, D. Stacey1, R. Mira de Orduña1,3; 1Univ. of Guelph, Guelph, ON, CANADA, 2ENSIA, Massy, FRANCE, 3Cornell Univ., Geneva, NY. 498. The Effect of Shear Forces on Filamentous Bacteriophage and the Implications for their Large-Scale Production S. D. Branston, E. C. Stanley, E. KeshavarzMoore, J. Ward;University College, London, UK. 489. Effect of Optimizing a Trace Metals Solution on Growth and Ethanol Production by Clostridium Strain P11 J. Saxena, M. W. Maune, R. S. Tanner; University of Oklahoma, Norman, OK. 499.Genome Shuffling of Saccharomyces cerevisiae for Resistance to Inhibitors from Lignocellulosic Substrates D. Pinel1, F. D'Aoust1, P. Bajwa2, T. Shireen2, H. Lee2, J. Trevors2, V. Martin1; 1Concordia University, Montreal, PQ, CANADA, 2University of Guelph, Guelph, ON, CANADA. 490. Mutants of the Pentose-Fermenting Yeast Pichia stipitis with Improved Tolerance to Spent Sulfite Liquor P. K. Bajwa1, T. Shireen1, F. D’Aoust2, D. Pinel2, V. J. J. Martin2, J. Trevors1, H. Lee1; 1University of Guelph, Guelph, ON, CANADA, 2Concordia University, Montreal, PQ, CANADA. 500. Lactobacilli and Tartrazine as Causative Agents of Red Color Spoilage in Cucumbers I. Díaz-Muñiz, R. E. Kelling, S. Hale, F. Breidt, R. McFeeters; USDA-ARS Food Science Research Unit, Raleigh, NC. 501. Biotransformation of Korean Panax Ginseng by Pectinex S. N. You, S. Y. Lee, H. J. Cho, Y. J. Kim, S. C. Ahn; Pusan National University School of Medicine, Busan, REPUBLIC OF KOREA. 491. Nisin Production in Bioreactor Using Skimmed Milk and Milk Whey A. F. Jozala, L. J. Arauz, P. G. Mazzola, A. Pessoa Jr., T. C. V. Penna; University of São Paulo, School of Pharmaceutical Sciences, São Paulo, BRAZIL. 502. Exopolysaccharide Production by Lactobacillus paracasei KLB58 in Modified Medium under Different Growth Conditions J-S. So1, C-Y. Lee1, S-C. Koh2; 1Inha University, Incheon, REPUBLIC OF KOREA, 2Korea Maritime University, Pusan, REPUBLIC OF KOREA. 492. Rapid Fermentation Process Research and Development for the Production of Natural Products A. Madjid-Yunus, D. B. Brzozowski, M. W. Chase, T. B. Phillips, P. C. Michels; Albany Molecular Research, Inc., Albany, NY. 503.Biotransformation and Biological Activity of Green Tea Extract Fermented by Aspergillus Oryzae H. J. Cho1, -6- Y. J. Kim1, J. K. Kim2, I. S. Kong2, S. C. Ahn1; 1College of Medicine Pusan National University, Busan, REPUBLIC OF KOREA, 2Pukyong National University, Busan, REPUBLIC OF KOREA. 514. Electrochemical Characterization of Shewanella Biofilms on Electrodes D. B. Baron; University of Minnesota, St. Paul, MN. 504.Isolation and Characterization of Antioxidant Substances Isolated from Bacillus polyfermenticus SCD Supernatant K-H. Chang, K-T. Kim, H-D. Paik; Konkuk University, Seoul, REPUBLIC OF KOREA. 515.Detecting Early Colonization of Surfaces by Geobacter Using Microfabricated Gold Electrodes L. J. Werner, V, E. Marsili, J. Alley, D. R. Bond; University Of Minnesota, St. Paul, MN. 516. Generation of Electricity by Thermophilic Microorganisms from Marine and Hypersaline Sediment B. J. Mathis1, C. E. Milliken1, S. E. Creager2, H. D. May1; 1 Medical University of South Carolina, Charleston, SC, 2 Clemson University, Clemson, SC. Poster: 049/O. Biocatalysis, Biotransformations and Bioremediation Description:O Fermentation & Biotechnology Time:5/22/2007 9:00:00 AM - 5/22/2007 12:00:00 PM 505.Effect of Multiple Copies of Cohesins on Cellulase and Hemicellulase Activities of Clostridium cellulovorans MiniCellulosomes J. Cha1, S. Matsuoka2, H. Chan3, R. H. Doi2; 1Pusan National University, Busan, REPUBLIC OF KOREA, 2University of California, Davis, CA, 3University of California Davis, Davis, CA. 506.Biocatalytic Matrices from Artificial Structural Proteins and Biosilification W. D. Marner, II, A. Shaikh, S. J. Muller, J. D. Keasling; U. of California, Berkeley, CA. 517.Cloning, Expression, and Characterization of a Novel Amylase Gene from a Thermophilic Bacterium Caldimonas Taiwanensis D-S. Sheu, W-M. Chen, W-T. Su; National Kaohsiung Marine Univ., Kaohsiung, TAIWAN. 518. Thermally Stable Amylases from Antarctic Psychrophilic Bacteria M. R. Smith; WRRC, USDA, Albany, CA. 519. Application of the Staphylococcus aureus CadmiumTransporter Cada In Zebrafish(Danio Rerio) for Heavy Metal Resistance K. Lin, Y-H. Li, C-C. Huang; Chung Hsing Univ., Taichumg, TAIWAN. 507. Characterization of the Alkene Monooxygenase from Nocardioides sp. Strain JS614 C. R. Owens, J. K. Karceski, T. E. Mattes; University of Iowa, Iowa City, IA. Poster: 148/O. Animal and Cell Tissue Culture 508.Mediatorless Bioelectrical Perchlorate Reduction J. Thrash1, L. A. Achenbach2, J. D. Coates1; 1University of California, Berkeley, CA, 2Southern Illinois University, Carbondale, IL. Description:O Fermentation & Biotechnology Time:5/23/2007 9:00:00 AM - 5/23/2007 12:00:00 PM 480.Improved Sialylation of Recombinant Glycoproteins in Chinese Hamster Ovary Cells by Co-Expressing CMPSialic Acid Transporter D. Kang, H. Cha; Postech, Pohang, REPUBLIC OF KOREA. 509.Isolation of Penicillin G Acylase from Escherichia coli ATCC 11105 by Physical and Chemical Treatments D. Abedi, H. Korbekandi, B. Haghighi, S. Kalantari; Isfahan University of Medical Sciences, Isfahan, IRAN (ISLAMIC REPUBLIC OF). 481.Real Time In Situ Monitoring of Mammalian Cell Culture Processes using Multiplexing Fibre Optic Near Infrared (NIR) Spectroscopy P. Roychoudhury1, R. D. O' Kennedy2, B. McNeil1, L. M. Harvey1; 1University of Strathclyde, Glasgow, UNITED KINGDOM, 2BioPharmCEDD, GlaxoSmithKline, Beckenham, UK. 510. Functional Metagenomic Analysis of Anaerobic Methane Oxidizing Communities M. Taupp, S. J. Hallam; University of British Columbia, Vancouver, BC, CA. 512. Degradation of the Primary Metabolite of 1,4-Dioxane Oxidation, 2-Hydroxy-Ethoxy-Acetic Acid, by Environmental Isolates K. McClay1, H. Musada2, G. J. Zylstra2, R. J. Steffan1; 1Shaw E&I, Lawrenceville, NJ, 2Rutgers University, New Brunswick, NJ. Poster: 149/O. Genetics, Gene Expression and Protein Production Description:O Fermentation & Biotechnology Time:5/23/2007 9:00:00 AM - 5/23/2007 12:00:00 PM 513. A New Laccase from Marasmius sp. for the Degradation of Textile Dyes A. Matura, J. Schückel, K-H. van Pee; TU Dresden, Dresden, GERMANY. -7- Abstract 482.Reconstituting the Plesiomonas shigelloides or the Vibrio cholerae Heme Transport System in Escherichia coli to Increase Hemoglobin Production C. L. Phillips1, D. M. Villarreal1, S. Villarreal1, A. M. Kelley1, J. S. Olson2, D. P. Henderson1; 1University of Texas, Odessa, TX, 2Rice University, Houston, TX. 493. Soluble Expression and Characterization of Recombinant Proline-Specific Peptidase from Lactococcus lactis S. I. Yang, T. Tanaka; University of Saskatchewan, Saskatoon, SK, CANADA. 483.Food Grade Cloning of Abortive Phage Resistance Determinant AbiZ E. Durmaz, T. R. Klaenhammer; North Carolina State University, Raleigh, NC. 494.The Physiological Impact of Penicillin Acylase Overproduction in Escherichia Coli N. Narayanan1, S. Follonier2, C. Chou1; 1University of Waterloo, Waterloo, ON, CANADA, 2Ecole Polytechnique Federal de Lausanne, Lausanne, SWITZERLAND. 484.Whole Genome Microarray and Genetic Analysis of Growth of Geobacter sulfurreducens on Fe(III) and Mn(IV) Oxides M. J. Larrahando, D. E. Holmes, R. A. O'Neil, M. A. Chavan, L. A. Adams, D. R. Lovley; University of Massachusetts, Amherst, MA. 495. Cloning of a Amylomaltase Gene from Deinococcus geothermalis and Enzymatic Characterization of Its Recombinant Protein Expressed in E. coli S-H. Hong1, MH. Lee1, M-Y. Baik1, S-C. Ahn2, C-S. Park1; 1Kyunghee University, Youngin-si, REPUBLIC OF KOREA, 2Busan Natl. Univ., Busan, REPUBLIC OF KOREA. 485. Detection and Amplification of NiFe-Hydrogenase Genes of Hydrogenovibrio marinus using Degenerate PCR and Heterologous Expression in Escherichia coli J. Y. H. Kim, H. J. Cha; Postech, Pohang, REPUBLIC OF KOREA. 497. Multiple Expression of Cellulase and Hemicellulase Enzymes from Clostridium cellulovorans in Bacillus subtilis WB800 S. Matsuoka, R. H. Doi; University of California, Davis, CA. 486.Characterization of a Putative Glutamine-Binding Protein from Thermotoga maritima M. S. Luchansky, J. D. Dattelbaum; University of Richmond, Richmond, VA. 487.Inducible Expression System in Rhodococcus M. Kobayashi, T. Ishikawa, Y. Hashimoto; The University of Tsukuba, Tsukuba, JAPAN. 498. The G Protein β Subunit Regulates Spore Germination, Vegetative Growth and Asexual Sporulation of Aspergillus Niger in Response to Nitrogen Sources Z. Dai; Pacific Northwest National Laboratory, Richland, WA. 488.A Markerless Gene Replacement System for Lactobacillus acidophilus NCFM with upp-Based Counterselection Strategy Y. Goh1, F. Valence2, D. Mollé2, S. Lortal2, T. R. Klaenhammer1; North Carolina State University, Raleigh, NC, 2INRA, Rennes, FRANCE. 499.Expression of Human Erythropoietin Having More Complex N-Glycans in Drosophila S2 Cells through Inhibition of Hexosaminidase Y. Kim, H. Cha; Postech, Pohang, REPUBLIC OF KOREA. 489. Development and Characterization of a Protein Expression System for Use in Lactobacilli T. Duong, M. J. Miller, R. Barrangou, T. R. Klaenhammer; North Carolina State University, Raleigh, NC. Poster: 150/O. Proteomics, Microarray Analysis and Genomics Description:O Fermentation & Biotechnology Time:5/23/2007 9:00:00 AM - 5/23/2007 12:00:00 PM 490.Characterization of a Novel Amylosucrase, a GlucanSynthesizing Enzyme, from Deinococcus geothermalis DSM 11300 D-H. Seo1, J-H. Jung1, S-h. Yoo2, C-S. Park1; 1KyungHee University, Korea, Youngin, REPUBLIC OF KOREA, 2Sejong University, Korea, Seoul, REPUBLIC OF KOREA. Abstract 500.Gene Expression Profile of Clostridium botulinum Using Microarray Y-H. Lin, W-J. Lin; California State Polytechnic University, Pomona, CA. 491. Enhanced Display of Yellow Fluorescent Protein on Escherichia Coli Surface by FkpA Coexpression L. Zhang, N. Narayanan, M. Moo-Young, C. Chou; University of Waterloo, Waterloo, ON, CANADA. 501. Proteomic Analysis of the Intercellular Fraction of Zymomonas Mobilis Responding to Ampicillin-Inducible Acetate Tolerance C. W. Hsieh, C. C. Chang; National ChaiYi University, ChaiYi, TAIWAN. 492. Overcoming the Tyrosine Production Bottleneck at Prephenate Dehydrogenase with Native Zymomonas mobilis tyrC or Mutant Escherichia coli tyrA(fbr) L. Templeton, C. Byrne, R. Jackson, D-I. Liao, T. Van Dyk; DuPont Company, Wilmington, DE. 502.Amino Acid Sequence Determination of Protein Biomarkers of Bacteria by "Composite" Sequence Proteomic Analysis C. K. Fagerquist; U.S. Department of Agriculture, Albany, CA. 503.Comparative Genomics In Identifying Aflatoxin Biosynthetic Genes J. Yu1, W. C. Nierman2,3, M. Machida4, -8- J. W. Bennett5, B. C. Campbell6, D. Bhatnagar1, T. E. Cleveland1, G. A. Payne7; 1USDA/ARS, New Orleans, LA, 2 The Institute for Genomic Research, Rockville, MD, 3The George Washington University, Washington, DC, 4AIST, Tsukuba, JAPAN, 5Rutgers University, New Brunswick, NJ, 6 USDA/ARS, Albany, CA, 7North Carolina State University, Raleigh, NC. M. Storm1,2, Q. Xiang1, S. Roos3, J. K. Spinler1,2, M. Janecki1,2, M. G. Dillon1,2, R. A. Britton4, J. F. Petrosino1, S. K. Highlander1, H. Jonsson3, G. M. Weinstock1,2, J. Versalovic1,2; 1Baylor College of Medicine, Houston, TX, 2 Texas Childrens Hospital, Houston, TX, 3Swedish University of Agricultural Sciences, Uppsala, SWEDEN, 4 Michigan State University, East Lansing, MI. 504. The Complete Genome Sequence of Bifidobacterium longum bv. infantis D. A. Sela1, S. L. Freeman1, P. M. Richardson2, J. B. German1, D. A. Mills1; 1 University of California, Davis, CA, 2Joint Genome Institute Production Genomics Facility, Walnut Creek, CA. 514.Experimental Determination of Protein-Protein Interactions in the Anoxygenic Phototrophic Bacterium Rhodopseudomonas palustris(Ctrl #: 3879) D. A. Pelletier1, G. B. Hurst1, L. J. Foote1, C. K. McKeown1, P. K. Lankford1, T-Y. S. Lu1, E. T. Owens1, D. D. Schmoyer1, M. B. Shah1, J. L. Morrell-Falvey1, B. S. Hooker2, S. J. Kennel1, W. H. McDonald1, M. J. Doktycz1, D. L. Auberry2, W. R. Cannon2, K. J. Auberry2, H. S. Wiley2, M. V. Buchanan1; 1Oak Ridge National Laboratory, Oak Ridge, TN, 2Pacific Northwest National Laboratory, Richland, WA. 505. Molecular Mapping and Strain Identification of Shiitake Mushroom Lentinula edodes P. Y. Yip, K. K. Chu, H. S. Kwan; The Chinese University of Hong Kong, Hong Kong, HONG KONG. 506. Involvement of Multiple Cell Surface Proteins in the Adherence of Lactobacillus acidophilus NCFM to Mucin R. Tallon1, L. Buck1, E. Altermann1, F. Valence2, D. Mollé2, S. Lortal2, T. R. Klaenhammer1; 1North Carolina State University, Raleigh, NC, 2INRA, Rennes, FRANCE. Poster: 151/O. Emerging Technology and Instrumentation Description:O Fermentation & Biotechnology Time:5/23/2007 9:00:00 AM - 5/23/2007 12:00:00 PM 507. Methods for Sequencing Low-Copy Extrachromosomal DNA in Soil Microbes K. S. Sampson, J. P. Zeigler, C. I. Rodriguez, D. J. Tomso; Athenix Corp, Durham, NC. Abstract 515.Effect of Moderate Electric Field Frequency and Harmonics on the Growth Kinetics and Metabolic Activity of Lactobacillus Aidophilus L. Loghavi, S. K. Sastry, A. E. Yousef; The Ohio State University, Columbus, OH. 508.Cloning of Mating-Type Genes of Lentinula edodes Based on Automatically-Annotated EST Sequences C. H. Au, C. L. T. Sham, W. W. Y. Chum, I. S. W. Kwok, H. S. Kwan; The Chinese University of Hong Kong, Shatin, HONG KONG. 516. Direct Linear Analysis: an Amplication- and CultureIndependent Method for Diagnosis of Human Infectious Diseases C. G. Conant, S. McLaughlin, E. T. Mollova, V. Patil, M. Zhang, D. Cameron, R. Gilmanshin; U.S.Genomics, Woburn, MA. 509.Sequence Analysis and Comparison of Multiple Plasmids from Soil Bacteria J. P. Zeigler, K. S. Sampson, C. I. Rodriguez, D. J. Tomso; Athenix Corp, Durham, NC. 510.Microarray Analysis of the Physiological State of Geobacter Species Growing in Subsurface Sediments L. A. Adams, D. E. Holmes, R. A. O'Neil, M. J. Larrahando, M. A. Chavan, D. R. Lovley; University of Massachusetts, Amherst, MA. 512.A Survey of Protein Post-Translational Modifications Found in the Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough: Search for Stress Response Mediators S. P. Gaucher1, G. S. Chirica1, R. Sapra1, A. M. Redding2, A. Mukhopadhyay2, G. M. Buffleben1, C. Kozina1, R. Phan2, D. C. Joyner2, J. D. Keasling2, T. C. Hazen2, A. P. Arkin2, A. K. Singh1, Virtual Institute for Microbial Stress and Survival; 1Sandia National Laboratories, Livermore, CA, 2Lawrence Berkeley National Laboratory, Berkeley, CA. 513.Comparison of Two Lactobacillus reuteri Genomes Reveal Divergence Within a Probiotic Species 517.Comparison of Whole Genome Amplification Technologies in Support of Diagnostic DNA Microarrays E. A. Bode, C. Chase, C. Gibson, J. Geyer, L. Wasieloski; USAMRIID, Frederick, MD. 518.Comparative Sequencing of Wild Type Caulobacter crescentus CB15 and its Mutant CB15N (NA1000) Using GS FLX TechnologyC. S. Perbost1, S. Crosson2, B. Desany1, G. Irzyk1; 1454 Life Sciences, Branford, CT, 2University of Chicago, Chicago, IL. 519.Direct Surface Immobilization Various Carbohydrate Types through Thiol-Group Coupling to Reducing Sugar J. Seo, H. Cha; Postech, Pohang, REPUBLIC OF KOREA. Looking forward to seeing you in Toronto! -9- Sincerely, The FermentOgram Editorial Board -10-