Standard Operating Procedures
Laboratory Specific
Chemical: Trinitroanisole
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:________________________
Date when SOP was written:_______
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
Historically, trinitroanisole was used as a military explosive, however, due to its tendency to form
picric acid and dangerous picrate salts, its use has largely been abandoned.
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
606-35-9
Class:
Explosive when dry & Toxic
Molecular formula:
C7H5N3O7
Boiling Point:
N/A (Explodes)
Melting Point:
68oC
Appearance:
Yellow, “leaf-like” crystals
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Potential Hazards/Toxicity
Explosive when dry and likely toxic.
Highly important danger information! - Dry trinitroanisole is relatively sensitive to shock, heat,
and friction, so it must be stored and used wet to reduce the risk of explosion.

Potential Health Effects: The toxicological properties of this material have not been
investigated, but it should be assumed to be toxic and contact should be avoided.
Personal Protective Equipment (PPE)

Eyes: Wear safety glasses.

Skin: Wear nitrile, natural rubber, neoprene, butyl, PVC or Viton gloves.

Clothing: Wear long pants, shirt, and closed toe shoes and a lab coat while handling.

Respirators: Respiratory protection is not generally required. Where protection from
nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks.
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2
requirements or European Standard EN 149 must be followed whenever workplace
conditions warrant respirator use.
Engineering Controls
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety
shower. Use adequate ventilation to keep airborne concentrations low.
First Aid Procedures

Inhalation: Remove to fresh air. If not breathing, give artificial respiration. If breathing is
difficult, give oxygen. Get medical attention.

Ingestion: Never give anything by mouth to an unconscious person. Rinse mouth with
water. Get medical attention immediately.

Skin Contact: Immediately flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Wash off with soap and plenty of water. Get
medical attention immediately. Wash clothing before reuse. Thoroughly clean shoes
before reuse. Contaminated work clothes should be laundered by individuals who have
been informed of the hazards of exposure to this substance.

Eye Contact: Immediately flush eyes with plenty of water for at least 15 minutes, lifting
lower and upper eyelids occasionally. Get medical attention immediately.
Special Handling and Storage Requirements

Handling: Wash thoroughly after handling. Remove contaminated clothing and wash
before reuse. Minimize dust generation and accumulation. Do not get in eyes, on skin, or
on clothing. Do not ingest or inhale. Use only with adequate ventilation or respiratory
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protection. Avoid shock, heat, and friction. Keep away from sources of ignition - No
smoking.

Storage: Store in a tightly closed container. Keep away from contact with oxidizing
materials. Store in a cool, dry, well-ventilated area away from incompatible substances,
heat, sparks, flames or other ignition sources.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Keep sparks, flames, and other sources of ignition away. Keep material wet. Wet spilled
material before picking it up. Do not attempt to sweep up dry material. Ensure adequate
ventilation. Evacuate personnel to safe areas. Pick up and arrange disposal without creating dust.
Keep in suitable, closed containers for disposal.
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
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Decontamination/Waste Disposal Procedure
Waste disposal procedures
General hazardous waste disposing guidelines:
Labeling Requirements for Hazardous Waste Containers:
• A UCLA Hazardous Waste Tag must be placed on each hazardous waste container upon
start of accumulation.
• The On-Line Tag Program (OTP) can be used to print hazardous waste tags right from your
printer.
• One account on the On-Line Tag Program (OTP) can be used for the entire lab. See the OnLine Tag Program1 more information and to get your lab signed up.
Hazardous Waste Storage:
• Hazardous waste must be transferred to EH&S for disposal within 90 days of being
generated.
• Waste containers must be in secondary containment at all times to adequately contain the
contents of the container/spilled materials.
• Hazardous waste must always be appropriately labeled with a UCLA waste tag at all times.
• Containers must be closed when not in use.
• Storage of hazardous waste in fume hoods or under sinks is not recommended.
• Hazardous waste that meets the quantity threshold of 55 gallons of hazardous waste or 1
quart of extremely hazardous waste1 must be transferred to EH&S for disposal within 3
days of reaching these set volumes.
• Report damaged containers to EH&S. EH&S can provide assistance to transfer the contents
to an appropriate container.
• Mark storage areas according to the type of chemicals kept there (e.g. “Corrosive”,
“Flammable”, etc.).
• Containers should be inspected weekly for signs of leaks, corrosion, or deterioration.
Hazardous Waste Disposal:
• Don't dispose of chemicals down the drain!
• Don't dispose of chemicals via trashcans.
• Don't use hoods to intentionally evaporate chemicals.
• Transport the hazardous waste to your designated pick-up location using a sturdy cart and
secondary containment.
• Consult the hazardous waste pick-up schedule1 for the building specific times and locations of
disposal.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
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Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
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