Standard Operating Procedures
Laboratory Specific
Chemical: 2,4-dinitrophenylhydrazine
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
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Department:________________________
Date when SOP was written:_______
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
2,4-dinitrophenylhydrazine (DNPH, Brady's reagent) is relatively sensitive to shock and friction. It
is a shock explosive so care must be taken with its use. It is a red to orange solid, usually
supplied wet to reduce its explosive hazard. It is a substituted hydrazine, and is often used to
qualitatively test for carbonyl groups associated with aldehydes and ketones.
In the Brady's test, 2,4-dinitrophenylhydrazine can be used to qualitatively detect the carbonyl
functionality of a ketone or aldehyde functional group. A positive test is signaled by a yellow or
red precipitate (known as a dinitrophenylhydrazone.) If the carbonyl compound is aromatic, then
the precipitate will be red; if aliphatic, then the precipitate will have a yellow color.
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
119-26-6
Class:
Explosive and flammable solid
Molecular formula:
C6H6N3O2
Physical Appearance:
Red Powder
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Boiling Point:
Not available
Melting Point:
200 deg C (decomposes)
Decomposition Temperature:
Not available
Potential Hazards/Toxicity
Target Organs: Lungs, nervous system, mucous membranes and blood
Potential Health Effects:
Eye: May cause eye irritation
Skin: Harmful if absorbed through skin. May cause skin irritation
Ingestion: Harmful if swallowed
Inhalation: May be harmful if inhaled. May cause respiratory tract irritation.
Chronic: Repeated or prolonged exposure to the substance can produce target organs damage
Personal Protective Equipment (PPE)
Eyes: Safety glasses with side-shields
Skin: Butyl gloves are highly preferred to prevent skin exposure.
Clothing: Wear long pants, closed toed shoes and a lab coat
Respirators: A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI
Z88.2 requirements or European Standard EN 149 must be followed whenever workplace
conditions warrant a respirator's use.
Engineering Controls
Use 2,4-dinitrophenylhydrazine in a fume hood to keep exposure to airborne contaminants below
the exposure limit.
First Aid Procedures
Eyes: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician
Skin: After contact with skin, wash immediately with plenty of water. Gently and thoroughly wash
the contaminated skin with running water and non-abrasive soap. Be particularly careful to clean
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folds, crevices, creases and groin. Cover the irritated skin with an emollient. If irritation persists,
seek medical attention. Wash contaminated clothing before reusing.
Ingestion: Call the poison control center at 1-800-222-1222. Do not induce vomiting. Examine
the lips and mouth to ascertain whether the tissues are damaged, a possible indication that the
toxic material was ingested; the absence of such signs, however, is not conclusive. Loosen tight
clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-tomouth resuscitation. Seek immediate medical attention.
Inhalation: Allow the victim to rest in a well ventilated area. Seek immediate medical attention.
Serious inhalation: Evacuate the victim to a safe area as soon as possible. Loosen tight clothing
such as a collar, tie, belt or waistband. If breathing is difficult, administer oxygen. If the victim is
not breathing, perform mouth-to-mouth resuscitation. Seek medical attention.
Notes to Physician: Treat symptomatically and supportively.
Special Handling and Storage Requirements
Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide
appropriate exhaust ventilation at places where dust is formed. Keep away from sources of
ignition – No smoking. Take measures to prevent the buildup of electrostatic charge.
Storage: Flammable materials should be stored in a separate safety storage cabinet or room.
Keep away from heat. Keep away from sources of ignition. Keep container tightly closed. Keep in
a cool, well-ventilated place. Ground all equipment containing material. Keep container dry. Keep
in a cool place.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Fire Fighting Media and Instructions – SMALL FIRE: Use dry chemical powder.
LARGE FIRE: Use water spray or fog. Cool containing vessels with water jet in order to prevent
pressure build-up, autoignition or explosion.
Spill – Sweep up and shovel. Contain spillage, and then collect with an electrically protected
vacuum cleaner or by wet brushing and place in container for disposal according to local
regulations (see section 13). Keep in suitable, closed containers for disposal. Contain spillage,
pick up with an electrically protected vacuum cleaner or by wet-brushing and transfer to a
container for disposal
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
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Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Waste disposal procedures
Follow UCLA EHS waste protocol
Labeling Requirements for Hazardous Waste Containers:
• A UCLA Hazardous Waste Tag must be placed on each hazardous waste container upon
start of accumulation.
• The On-Line Tag Program (OTP) can be used to print hazardous waste tags right from your
printer.
• One account on the On-Line Tag Program (OTP) can be used for the entire lab. See the OnLine Tag Program1 more information and to get your lab signed up.
Hazardous Waste Storage:
• Hazardous waste must be transferred to EH&S for disposal within 90 days of being
generated.
• Waste containers must be in secondary containment at all times to adequately contain the
contents of the container/spilled materials.
• Hazardous waste must always be appropriately labeled with a UCLA waste tag at all times.
• Containers must be closed when not in use.
• Storage of hazardous waste in fume hoods or under sinks is not recommended.
• Hazardous waste that meets the quantity threshold of 55 gallons of hazardous waste or 1
quart of extremely hazardous waste1 must be transferred to EH&S for disposal within 3
days of reaching these set volumes.
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• Report damaged containers to EH&S. EH&S can provide assistance to transfer the contents
to an appropriate container.
• Mark storage areas according to the type of chemicals kept there (e.g. “Corrosive”,
“Flammable”, etc.).
• Containers should be inspected weekly for signs of leaks, corrosion, or deterioration.
Hazardous Waste Disposal:
• Don't dispose of chemicals down the drain!
• Don't dispose of chemicals via trashcans.
• Don't use hoods to intentionally evaporate chemicals.
• Transport the hazardous waste to your designated pick-up location using a sturdy cart and
secondary containment.
• Consult the hazardous waste pick-up schedule1 for the building specific times and locations of
disposal.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
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