Mendel’s Peas Please Ideal Course for Lesson to be Taught: Advanced High School Biology Length of Lesson: 90 minutes Authors: Amanda Maez Lesson overview: This lesson not only introduces students to the basics of genetics including: Inherited Traits, Alleles, Dominant and Recessive Traits, Hybridization, Self-Pollination and Cross-Pollination, and F1 and F2 Generations, but also gives them a historical framework from which to draw. It is based around Gregor Mendel’s (1822-1884) original experiments on pea plants and the numerical relationships between traits. It allows students to first examine some of their own inherited traits, and then use historical information to discover why inheritance acts in the way it does. Performance objectives – students will be able to: -Understand the basics of genetics and inheritance based on Gregor Mendel’s original experiments with pea plants. -Evaluate data from a hypothetical experiment with pea plants. -Predict the outcome of certain genetic crosses. -Explain why Gregor Mendel’s work was so foundational to the history of science. Standards Addressed: History Standard: The student uses a working knowledge and understanding of significant individuals, groups, ideas,events, eras, and developments in the history of Kansas, the United States, and the world, utilizing essential analytical and research skills. Benchmark 2: The student uses a working knowledge and understanding of individuals, groups, ideas, developments, and turning points of the Age of Revolutions (1650-1920). Benchmark 5: The student engages in historical thinking skills. LIFE SCIENCE – The student will develop an understanding of the cell, molecular basis of heredity, biological evolution, interdependence of organisms, matter, energy, and organization in living systems, and the behavior of organisms. Benchmark 2: The student will demonstrate an understanding of chromosomes, genes, and the molecular basis of heredity. 3. ▲ understands hereditary information is contained in genes, located in the chromosomes of each cell. Lesson Bibliography: Gregory, Frederick. Natural Science in Western History. Vol. 2. Boston, MA: Houghton Mifflin, 2008. Print. Newton to the Present. Stark, Louisa, Molly Malone, and Harmony Starr. "Inherited Human Traits: A Quick Reference." Learn.Genetics™. Howard Hughes Medical Institue, 2008. Web. 10 Apr. 2011. <http://learn.genetics.utah.edu/>. Materials: -One copy of “Inherited Human Traits: A Quick Reference” for each student in the class -PTC Taste Paper (one strip/student) -One copy of “Learn.Genetics Worksheet” for each student -One data sheet for each student -One Evaluation for each student Safety considerations: Students should abide by all classroom safety rules. When ordering the PTC paper for testing, make sure it is from a reliable source and contains only from .005-.007mg of PTC, as large amount of this chemical can be toxic (As long as the substance is regulated it is not harmful.) Make sure each student only gets one piece of PTC paper. Note: if there is concern about including PTC in the lesson, just leave it out. There is much research to show that it is just about as toxic as table salt if used in large amounts, but its use is at the teacher’s discretion. Engagement Time: 15 minutes What the teacher will do Give each student a copy of “Inherited Human Traits: A Quick Reference” and a copy of “Are you Dominant or Recessive?” Worksheet Write ‘INHERITED TRAIT’ on the board in front of the class What the teacher will say Probing/eliciting questions Specific possible student responses Who can tell me what an inherited trait is? Skin color, eye color, hair color, a disease, physical characteristics (this will be the first of several vocabulary words the students will encounter today) determined by genes And who do we inherit these traits from? Put students into random groups of three, and direct their attention to the articles. Our parents, grandparents, etc. That’s right, our traits are passed down to us from our parents. This article contains Can someone tell me Dominant and several traits that are what the two types of Recessive; widow’s easily recognizable. traits named in the peak background information section are? Write ‘DOMINANT’ and ‘RECESSIVE’ on the board Does anyone else recognized any other vocabulary words in the background information that they have not seen before? Allele; genes; observable characteristics; conception; As students mention ‘ALLELE’ and ‘GENE’, write them on the board. Instruct the students to begin going through the article and filling out the worksheets based on whether they are dominant or recessive for each trait. (Allow 10 minutes) Students may struggle with being able to determine which allele combinations they could possibly be. Walk around the room and address questions about allele combinations and other questions, but save the discussion about alleles with the whole class for the EXPLAIN. Transition to the history aspect and EXPLORE portion of the lesson. Does anyone know 1998; ancient Greece; when scientists first various answers started to think about genes and inherited traits? Exploration Time: 15 minutes What the teacher will do What the teacher will say Probing/eliciting questions Specific possible student responses Introduce Gregor Mendel’s experiments with pea plants and tell the students they will be conducting similar studies to Mendel’s by observing data from different Before anyone knew anything about DNA or genes, a monk from Austria named ‘GREGOR MENDEL’ pioneered the field of ‘GENETICS’ in the 1800s. Does anyone have any idea how he might have done this without the use of microscopes or any of the technology scientists have today? No; maybe he used animals?; I don’t know hybridization crosses in pea plants. Can anyone think of Bugs; plants! something that has observable characteristics, but reproduces much faster than a human or an animal (in other words, many generations can be observed over the course of several years)? Yes! Mendel studied plants, fairly simple plants in fact. At his monastery, they gave him a garden that was 120 feet by 20 feet where he studied about 30,000 pea plants in eight years. As a vocabulary term comes up, write it on the board. With the students still in their groups of three, hand each student a worksheet with “data” from Mendel’s experiment. On the back of the worksheet should be the list of Mendel Selected and organized his plants based on seven characteristics, but we will only use two of those characteristics today. (seed color and seed shape). vocabulary terms for the day. Without knowing the genetic code and the specific DNA sequence and without being able to see the genes themselves, how do you think we will be able to determine what is dominant and what is recessive? Yes, we’re going to look at the characteristics of the different generations of plants, just like Mendel did, and determine which alleles they have. Have the students work together to determine how dominant and recessive alleles act on physical traits, and then predict the results of certain crosses of alleles. Be sure to walk around the room and help the students as they are filling out the worksheets since this is presumably the first time they will be working on genetic crosses. We know that traits are determined by the action of two alleles. Some alleles are dominant and some are recessive. Each of the traits on the worksheet shows 1 of 2 phenotypes (or physical trait determined by genetics)—Dominant or Recessive. Try to determine the 2 alleles of each of the parents based on the phenotypes of the offspring generations. By looking at the characteristics of the plants. Have them try to figure out the alleles of the parental generations on their own first, but if they are stuck, try to lead them to the correct answer. Do you notice anything about the ratios of a certain trait to another trait? What is the best way of organizing your data? Yes, the F2 Generations all have 3:1 Dominant:Recessive Putting it in tables, putting it in lists… Explanation Time: 10 minutes What the teacher will do What the teacher will say After students have completed the inheritance of peas assignment, first discuss the vocabulary terms in the list. Before we discuss our Mendel worksheets, let’s talk about some of the vocabulary terms that we have come across so far in class today. So I would like everyone to take out their Vocabulary worksheets and we will fill them in together. Probing/eliciting questions Specific possible student responses Can someone hypothesize a definition for one of the vocabulary words? I think that dominant means that most people have that trait… That’s a good guess, actually, that’s not always the case with traits. The fact that a trait is dominant does not mean it’s common, it just means it will win out over recessive. The definition of DOMINANT is… (use definitions from the students’ textbooks or definitions you plan on using in assessment, so that they can refer back to them and do not get confused) Go through all of the terms on the list, thoroughly, but quickly in lieu of time. (it would be a good idea to have a list filled out before class to place on an overhead, so that the students can refer to it as each term comes up in discussion) Now discuss why they were able to predict the outcomes of certain crosses of alleles. Now let’s refer back to our worksheet with Mendel’s data. Can somebody tell me what they predicted for a parental generation based on the characteristics of Ya, for the first one, we think that one parent has both dominant alleles and the other has both VERY GOOD! You have just done exactly what Mendel did! He discovered that phenotypes were not the offspring generations? recessive. What do you think about the second cross? We think this parent has one dominant and one recessive allele and this parent has two dominant alleles Ok great, can you tell me how you came to that conclusion? Well, we thought that both of the parents were both dominant for both alleles at first because both parents have the dominant trait, but then when you look at the F1 and F2 generations, there are recessive traits present too, so we figured that one of the parents has to have on recessive allele. always what were expected, and he was able to explain why that happened, and he came up with a law about crossing different traits that we still use today. Discuss how Mendel’s work was foundational in the area of genetics and how the numerical relationships between traits that he discovered allowed him to predict certain outcomes even though he didn’t actually know all the was contained within a gene. Mendel was one of the founding fathers of genetics, and he was able to do something that has lasted for decades. Why do you think his work was so foundational? I don’t know, he just grew plants…. Well did Mendel know that traits were passed No on by genes? Did he know that each trait was determined No; Maybe; I don’t by two alleles? know Did he has a microscope to look at the DNA of his plants? You’re right. Mendel didn’t have any of the technology that we have today. He made hypotheses based on what he observed in nature. Correct! Mendel made a prediction that was able to be Can anyone tell me why Mendel’s hypothesis was a good one? No It was testable and observable. tested and reproduced. The other thing about Mendel’s experiment was that he gathered a lot of data! The more data you gather, the more convincing your conclusions will be. Mendel is a very important scientist because he laid the foundations for the study of genetics, and the work he did is still used today! Elaboration Time: 7-9 minutes What the teacher will do What the teacher will say Probing/eliciting questions Specific possible student responses Have the students separate into groups of two and work at a computer in pairs. If students finish early with the EXPLORE, they can begin working on this portion of the lesson while other groups finish up. Direct the students to http://learn.genetics.utah.edu/ As they tour the interactive website, have them fill out a “Learn.Genetics” worksheet As you explore this website, be sure to take notes, and think about what you Students will comply. have learned about genes and genetics and how that can be applied to some of the things on this website. Evaluation Time: 8 minutes What the teacher will do What the teacher will say Probing/eliciting questions Hand out an evaluation to each of the students and answer questions they may have about the wording of a question. Specific possible student responses Students will work on their evaluations until the class period is over. Name:_________________ Mendel’s Peas Please—Evaluation Please select 3 of the 11 vocabulary words to define in your own words. Inherited Trait Allele Gregor Mendel Genetics Dominant Hybridization Self-Pollination Cross-Pollination Recessive F1 Generation F2 Generation 1. 2. 3. When and where did Gregor Mendel study pea plants? a. 1860s in New Zealand b. 1980s in France c. 1860s in Austria d. 1900 in Germany What are some important aspects of Mendel’s original experiment that made it so successful? a. Used a large variety of species. b. Selected varieties that differed in but one or a few characteristics, so that he could isolate the effect on the traits in the offspring. c. Summarized the relations by comparing how many showed which traits (tried to show a numerical ratio). d. Preformed experiments on only one generation. e. A, B and C f. B and C only g. all of the above Why was Gregor Mendel’s work with pea plants so important in the history of science? Name:______________________ Trait Example Earlobe attachment Tongue Rolling Cleft Chin Dimples Handedness Freckles Naturally Curly Hair Hand Clasping Color Blindness Hairline Shape PTC tasting Dominant X Recessive Possible Allele Combination(s) Aa, AA What alleles do you think your parents have? Your grandparents? Why? Can you think of other traits you may have gotten from your parents? Term Inherited Trait Dominant Recessive Inheritance Allele Gregor Mendel Genetics Hybridization Self-Pollination Cross-Pollination F1 Generation F2 Generation Definition Name:_______________________ Instructions: 1. Go to http://learn.genetics.utah.edu/. 2. Feel free to look around and click on some areas that interest you about genetics (Basics, DNA, cells, technology, virtual labs) 3. Tell me about what you learned: Which area did you spend the most time learning about? ____________________________________________________________________ Please list a few new vocabulary words you learned and their definitions (if you are unsure of their definitions, look them up in your textbook or online): ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ What was the most interesting thing you learned about genetics from this website? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ How can you apply what we learned today about genetics to what you learned on the website? (“I cannot apply anything” is NOT a sufficient answer.) ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________