APPLICATION FOR ANNUAL CONTINUATION OF AN APPROVED PROTOCOL
Yale University
Institutional Animal Care and Use Committee (IACUC)
Approval for animal protocols may continue for a period of up to 3 years. Federal requirements
mandate that protocols be reviewed no less than annually.
PI NAME:
PROTOCOL#:
TITLE OF PROJECT:
IF YOU PLAN TO TERMINATE THE PROJECT
If this project is NOT to be continued, please sign and date where indicated and the protocol will be
terminated as of the expiration date noted in the Annual Renewal Notification email. Once this protocol
is terminated, no further animal work may continue on this protocol and all animals on the protocol must
be moved to another appropriate, approved and active protocol or euthanized by the termination date.
I do not wish to continue this study and understand that by terminating this protocol, all animal work on
this project must stop and all animals removed from the protocol by the expiration date:
PI Name (Typed or Printed)
PI Signature
Date
*Please note: projects that are terminated require above signature ONLY. Do not complete the
remainder of the form and return this as a .pdf file to iacuc@yale.edu.
If you are requesting approval for annual continuation for the above project, please
provide the information requested on the attached Application. If a specific section of
this application does not apply to your project, please indicate that it is not applicable
(NA).
Please complete this application and submit as a .pdf file to iacuc@yale.edu.
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APPLICATION FOR ANNUAL CONTINUATION OF AN APPROVED PROTOCOL
If you are requesting approval for annual continuation, please provide the following information:
a. Did unexpected adverse events occur resulting in increased pain, distress or death rates to
live vertebrate animals in the study? If so, how were they managed and what steps were taken to
prevent recurrence (if applicable)?
b. Have animal numbers been reduced by a change in experimental design or technique since
the last review? If yes, please describe (this is being asked in order to comply with regulations,
documenting efforts to reduce the number of animals used):
c. Have any refinements been made to the study that would minimize potential pain, distress
and discomfort since the last review? If yes, please describe (this is being asked in order to
comply with regulations, documenting efforts to refine procedures/replace live animals with nonanimal models):
d. If you have been approved for the use of a general class of radio- and non-radioactive tracers,
agonists, and/or antagonists and have used any of these reagents/drugs during the last year,
please provide a list of the agents with the dose range that was used for each and a list of any
adverse events that occurred. If adverse events did occur, how was the health and welfare of the
animal addressed?
e. If genetically altered animals were produced please answer the following four questions:
1. For each transgenic or knockout strain, how many animals of the desired phenotype were
made since the last review?
2. How many animals were used to obtain the above number?
3. How many animals were produced that were not the desired phenotype?
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4. If unexpected (adverse) phenotypes were produced, how were health and welfare of the
animals addressed? Identify the phenotype(s).
f. Please provide confirmation that those personnel who were to receive training on
procedures/animal handling since the last review were trained and by whom. Please list the
personnel and who trained them:
g. Are there any changes to be made at this time to the approved protocol?
NO If NO submit this original form only.
YES If YES, please submit an Application for Modifications to an Approved Protocol that can
be found at http://iacuc.yale.edu/forms/index.html and follow the Modification Instructions.
Submit this application separately to IACUC@yale.edu.
OFFICE USE ONLY
Reapproval for use of laboratory animals in this project is granted.
Approval period is as follows:
From: ____________________________ to _______________________________
Signature of IACUC Chair or designee
Date
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