The Effects of Fatigue on Balance on a Cycle Ergometer Purpose The purpose of this experiment was to measure the effects of fatigue when riding a racing bicycle on a roller ergometer. Methods Subjects Subjects comprised 10 experienced male cyclists who had at least 3 years of international competitive racing experience. Their mean ± SD age, height and body mass was 25.3 ± 2.7 yr, 165.8 ± 12.7 cm and 69.6 ± 3.9 kg, respectively. Figure 1. Roller ergometer. Study Design The study was a repeated measures design in which balance measurements were recorded every 3 minute while the subjects completed a fifteen minute fatigue cycle ride using a roller ergometer. Reproducibility of balance measurements were measured in a separate reliability study. Procedures The subjects rode a racing bicycle on a roller ergometer, see Figure 1. The bicycle’s front and rear wheels were place on the tops of cylindrical rollers so that as the wheels turned, the rollers turned and the rider was able to ride in place. A 4-inch wide stripe was painted in the middle of the front roller, and the rider was required to keep the front wheel on the stripe. The rear rollers were connected to a braking system to provide resistance to the rear wheel of the bike. Balance was indicated by wobble in the front wheel and was measured by counting the number of times per minute that the front wheel of the bike strayed off the 4” stripe. As resistance on the rear wheels increased, physiological fatigue increased, and it became more and more difficult to maintain the front wheel on the stripe. Subjects rode for 15 minutes, divided into five 3-minute periods for the purpose of collecting data. Data were collected on the number of balance errors during the last minute of each 3-minute period, and resistance was increased at the end of each 3-minute period. In this design, the dependent variable is balance errors and the independent variable is increase in resistance (fatigue). Measurement of Reliability Prior to initiating the study, five subjects (not in the experiment), participated in a testretest assessment of measurement reliability. Subjects in the reliability study rode the bicycle for 15 min and their balance was measured at 3, 6, 9, 12 and 15 minutes. Reproducibility of balance was analyzed using SPSS (12.0 for Windows) to compute the intraclass correlation coefficient (ICC) using a two factor mixed effects model and type consistency (McGraw and Wong, 1996; Shrout and Fleiss, 1979). A high degree of reliability was found between balance measurements, Single Measure ICC = .997 with a 95% confidence interval from .915 - .997. A Priori Statistical Power Analysis Based on initial pilot testing of five subjects (or existing published data), we determined a practical statistical significant difference in balance of 12 ± 11. For this design, 11 subjects would be required to obtain 0.80 statistical power (Cohen, 1988). Statistical Analysis Data were analyzed using SPSS version 12.0.1 for Windows. Values are expressed as means ± SD. The distribution of each variable was examined with the Kolomogorov-Smirnov and Shapiro-Wilk normality tests. A single factor repeated measures ANOVA was performed to detect significant differences in balance by levels of fatigue (3, 6, 9, 12 and 15 minutes). Level of fatigue (time in minutes) was a within subjects factor. Follow-up tests of significant ANOVA effects were compared using the Tukey post hoc test. The level of significance was set at P < 0.05. Results The means ± SDs for balance by level of fatigue (3, 6, 9, 12 & 15 min) are shown in Figure 2. The K-S and S-W tests of normality indicated that the variables were normally distributed. Mauchly’s test of sphericity indicated that the assumption of sphericity was violated, therefore, the degrees of freedom for all ANOVAs were adjusted using the Greenhouse-Geisser adjustment. The level of fatigue significantly effected balance when riding a racing bicycle on a roller ergometer [F(1.485, 13.367) = 18.36, p = 0.000, observed power = .995, effect size or partial η2 = .67]. {Note: without using the G-G adjustment the stats would have been F(4,36) = 18.36, p = 0.000, observed power = 1.000, effect size or partial η2 = .67} As shown in Figure 1, a significantly greater number of balance errors occurred at 12 min (31.1 ± 12.6) than: 9 min (16.4 ± 10.8), 6 min (11.4 ± 7.96) and 3 min (8.5 ± 4.5). The number of balance errors at 15 min (36.5 ± 21.1) was significantly greater than: 9 min (16.4 ± 10.8), 6 min (11.4 ± 7.96) and 3 min (8.5 ± 4.5). No other pairwise comparisons were significant. 70 Figure 2 Balance Errors 60 50 40 a,b 30 a,b 20 a,b 10 0 0 5 10 15 20 Minutes of Exercise a indicates means are significantly different from 12 minutes. indicates means are significantly different from 15 minutes p < 0.05 b Tables and Figures should ALWAYS have a note indicating groups (means) that are significantly different, even if there are NO SIGNIFICANT DIFFERENCES. References Cohen J. Statistical Power Analysis for the Behavioral Sciences, Lawrence Erlbaum Associates: Hillsdale, NJ, 1988. McGraw KO and Wong SP. Forming inferences about some intraclass correlation coefficients. Psychological Methods 1: 30-46, 1996. Shrout PE and Fleiss JL. Intraclass correlations: Uses in assessing reliability. Psychol Bull 86: 420-428, 1979.