Specifications Agilent 1200 Series Isocratic Pump
Flow
Precision
Flow accuracy
≤0.07 % RSD,or ≤0.02 min SD whatever is greater, based on retention time at constant room temperature
± 1 % or 10 µL/min whatever is greater
Range
Setpoints from 0.001 to 10.0 mL/min, in 0.001 mL/min increments
Pressure
Operating range
Pulsation
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi) up to 5 mL/min
0 - 20 MPa (0 - 200 bar, 0 - 2950 psi) up to 10 mL/min
<2 % amplitude (typically <1 %) at 1 mL/min isopropanol at all pressures >1 MPa (> 10 bar, >147 psi)
User selectable, based on mobile phase compressibility Compressibility compensation
Recommended pH range
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded.
An optimized design ensures virtually pulse-free and stable solvent flow, with dual floating pistons in series, precisely servo-controlled. This delivery principle allows variable stroke volume optimized to selected flow rate. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation with volatile solvent and is maintenance free.
Features and Benefits

Ease-of-use - the Agilent 1200 Series instant pilot allows you to enter operating parameters fast, store methods easily and display pressure profile, diagnostics and task-oriented information.

Robust materials withstand most demanding applications - stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE ensure long lifetime. Seal wash option for high salt mobile phases keeps maintenance to a minimum for lower operation costs.

Versatile wide flow range up to 10 mL/min for broad HPLC work, including semipreparative separations.

More help for GLP - built-in leak test with graphical presentation of pressure profiles, and early

Low operating and maintenance costs - due to long instrument uptime.
 maintenance feedback.
Future proof - upgradeable to quaternary pum

Specifications Agilent 1200 Series Binary Pump
Flow
Flow precision
Flow accuracy
Range
Pressure
<0.07 % RSD,or ≤0.02 min SD whatever is greater, based on retention time at constant room temperature
± 1 % or 10 µL/min whatever is greater
Setpoints from 0.001 to 5.0 ml/min, in 0.001 ml/min increments
Operating range
Pulsation
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi)
<2 % amplitude (typically <1 %) at 1 ml/min isopropanol at all pressures >1
MPa (> 10 bar, >147 psi)
Compressibility compensation User selectable, based on mobile phase compressibility
Gradient
Delay volume
Composition precision
180 - 480 µl (600-900 µl with mixer) dependent on back pressure
<0.15 % RSD, 1 mL/min
<0.5 % absolute Composition accuracy
Recommended pH range
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded
Whether operating standalone with the Agilent 1200 Series instant pilotor as part of an Agilent
ChemStation-controlled HPLC system, the Agilent 1200 Series binary pump is designed for top performance high-pressure mixing. It is the pump of choice for reproducible gradients and high performance, especially at low flow rates and for high throughput applications. The Agilent 1200 Series binary pump has an optional programmable solvent selection valve which combines two out of four solvents for utmost flexibility and increased convenience, for example, when flushing the column.
Features and Benefits

Low internal and external volumes of capillary connections and a minimum of bench space needed due to two dual-piston, in-series pumps in one housing.

Meeting highest chromatographic demands by using servo-controlled pistons of both individual pumps.
 A wide flow range starting at typically 50 µL/min for reproducible gradients at low flow with solvent degassing.


Reliable design keeping maintenance to a minimum using electronically-controlled active inletvalves for flow rates up to 5.0 mL/min. the binary pump is also ideally suited to do standard-bore chromatography. Floating pistons with path-of-least-resistance principle ensures highest seal lifetime.
Specifications Agilent 1200 Series Quaternary Pump
Flow
Flow precision
Flow accuracy
≤0.07 % RSD,or ≤0.02 min SD whatever is greater, based on retention time at constant room temperature
± 1 % or 10 µL/min whatever is greater
Setpoints from 0.001 to 10.0 ml/min, in 0.001 ml/min increments Range
Pressure
Operating range
Pulsation
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi) up to 5 ml/min
0 - 20 MPa (0 - 200 bar, 0 - 2950 psi) up to 10 ml/min
<2 % amplitude (typically <1 %) at 1 mL/min isopropanol at all pressures
>1 MPa (> 10 bar, >147 psi)
Compressibility compensation User selectable, based on mobile phase compressibility
Gradient
Delay volume 800 - 1100 µL depending on back pressure
Composition precision
Recommended pH range
<0.20 % SD, at 0.2 and 1 mL/min
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded.
The optimized design of the Agilent 1200 Series quaternary pump with incorporated vacuum degasser ensures virtually pulse-free and stable solvent flow, with dual floating pistons in series, precisely servo-controlled. This design with variable stroke volume allows pulse-free solvent delivery and efficient mixing. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation with volatile solvents and is maintenance free. The Agilent 1200 Series quaternary pump provides highest flexibility in solvent mixing and is recommended for a wide range of research and routine applications, and in the method development area.
Features and Benefits

Speeding up method development, preparation of mobile phases and flushing the HPLC system by offering convenient access of up to four solvents for isocratic or gradient analysis.


Keeping maintenance to a minimum for lower operation costs by using robust materials such as stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE that withstanding most demanding applications ensuring long lifetime. The optional active seal wash option is recommended for high salt mobile phases to prevent corrosion.

A wide flow range of up to 10 mL/min for broad HPLC work, including semipreparative separations.

Easy programming and control through the handheld controller, instant pilot, or through different
Agilent data systems, e.g. the Agilent ChemStation.

More help for GLP using the built-in leak and pressure test with graphical presentation of pressure profiles, and early maintenance feedback.
Injection range
Precision
Performance Specifications 1200 Series standard autosampler
Valid when standard 100
µL metering head installed
0.1-
100 µL in 0.1 µL increments
Up to 1500 µL with multiple draw (hardware modification required)
<0.25% RSD from 5-
100 µL, <1% from 1-5 µL, variable volume
Sample viscosity range
Sample capacity
0.2 - 5 cp
100 × 2-ml vials in 1 tray
40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)
Injection cycle time Typically 50 s depending on draw speed and injection volume
Carry-over Typically < 0.1 %, < 0.05 % with external needle cleaning
Can be upgraded from non-thermostatted version to thermostatted version by adding the Agilent 1200
Series thermostat for ALS/FC/spotter
Features
 Reliable injections from 0.1 µL to 100 µL
 Easy adaption for injection volumes up to 1500 µL for applications ranging from microbore to semipreparative chromatography
 Low internal volume of 300 µL for the minimum contribution to a system's total internal volume can be even further reduced using "by-pass" mode

Overlapped injections for increased productivity
Sample capacity
 100 × 2-mL vials in a standard tray
 40 × 2-mL vials in a standard tray
 15 × 6-mL vials in a half tray
 Microvials (100 or 300 µL) with sleeves

Detection type
Light source
Noise
Drift
Linearity
Wavelength range
Wavelength accuracy
Wavelength bandwidth
Maximum data rate
Flow cells
Specifications 1200 Series Variable Wavelength Detector
Double-beam photometer
Deuterium lamp
± 0.75 x 10 -5 AU, at 254 nm
3 x 10 -4 AU/hr, at 254 nm
>2 AU upper limit
190-600 nm
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
6.5 nm typical over whole wavelength range
13 Hz
Standard: 14-
µL volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum
High Pressure: 14-
µL volume, 10-mm cell path length and 400 bar (5880 psi) Pressure maximum
Micro: 1-
µL volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum
Semimicro: 5-
µL volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
Communications
GLP
Safety and maintenance
Controller-area network (CAN), RS-232. APG Remote:ready, start, stop and shut-down signals, LAN optional
Extensive diagnostics, error detection and display through control module and Agilent ChemStation, leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded. Verification of wavelength accuracy with built-in holmium oxide filter
Whether as a standalone detector extending your analytical range, or in an automated Agilent 1200 Series system enhancing your productivity, the Agilent 1200 Series variable wavelength detector (VWD) lowers detection limits with simplified optics for high sensitivity, low baseline drift and wide linear range. Lower your cost of ownership with easy front access to lamp and flow cell for fast replacement. Helps to comply to GLP with built-in holmium oxide filter for automatic wavelength verification.
Features and Benefits


Achieve high sensitivity and low detection limits with low baseline noise and drift.
Cut operating costs with automatic shutdown for longer productive lamp lifetime.


Verify wavelength accuracy for GLP compliance - automatic holmium oxide filter can be set at the beginning of your chromatography to verify that your wavelength is indeed what the setpoint says it is.

Realtime chromatographic signal plot and built-in context-sensitive online information with easy step-by-step guidance on handheld control modules (Agilent 1200 Series handheld control module or the new Agilent 1200 Series Instant Pilot).

Highest uptime through simplified design.
Detector type
Light source
Short-term noise
Drift
Linearity
Wavelength range
Slit width
Diode width
Wavelength bunching
Flow cells
Standard
Semi-micro
Micro
Specifications Agilent 1200 Series Multiple Wavelength Detector
Wavelength accuracy
1024-element diode array
Deuterium and tungsten lamp
± 0.8 x 10 -5 AU at 254 nm and at 750 nm
0.9 x 10 -3 mAU/hr, at 254 nm
>2 AU upper limit
190-950 nm
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
Programmable: 1, 2, 4, 8, 16 nm
< 1 nm
Programmable, 2 - 400 nm, in steps of 1 nm
Semi-nano
Nano
High pressure
Preparative
Analog output
Time programmable
13µL volume, 10 mm-cell path length, 120 bar (1760 psi) pressure maximum
5-
µL volume, 6-mm cell path length, 120 bar (1760 psi) pressure maximum
2µL, volume, 3-mm cell path length, 400 bar (5880 psi) pressure maximum
500-nanoliter volume., 10-mm cell path length, 50 bar (735 psi) pressure maximum
80-nanoliter volume, 6-mm cell path length, 50 bar (735 psi) pressure maximum
1.7-
µL volume, 6-mm cell path length, 400 bar (5880 psi) pressure maximum
3-mm cell path length, 120 bar (1760 psi) pressur maximum
0.3-mm cell path lenght, 20 bar (294 psi) pressure maximum, quartz
0.06-mm cell path length, 20 bar (294 psi) pressure maximum, quartz
Wavelength, polarity, peak width, lamp bandwidth, autobalance, wavelength range, threshold, spectra storage mode
Recorder/integrator 100 mV or 1 V, 2 outputs

Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded. Verification of wavelength accuracy with built-in holmium oxide filter
The Agilent 1200 Series multiple wavelength detector allows a wide range of wavelengths from low UV to the visible range. The diode array based dual lamp design provides high sensitivity for trace-level quantification. Simultaneously compounds of interest can be quantified and impurities are monitored at additional wavelengths. It is recommended for the pharmaceutical QA/QC laboratory, bioscience applications (proteins, DNA) and as a general purpose UV detector for routine work The temperature management system ensures optimum baseline stability –even under rough ambient conditions.
Features and Benefits

Excellent signal-to-noise
– for lowest limits of detection

Up to five signals
– enables highest selectivity without loss of sensitivity

Extended wavelength range
– enables a wide range of applications from low UV to the visible range maintaining a low noise level

Tools for conventient GLP – for example, built-in wavelength calibration and automated OQ/PV procedures

Upgradeable to full spectral capabilities
– to protect your investment today
Low cost of ownership - long life deuterium lamp (>2000 h), graphical ChemStation user interface to speed up training time, built-in diagnostics software, easy front access to lamp and flow cell for fast replacement.
Detector type
Specifications Agilent 1200 Series Diode-Array Detector
1024-element diode array
Light source
Short-term noise
Deuterium and tungsten lamp
± 0.8 x 10 -5 AU at 254 nm and at 750 nm
Drift
Linearity
0.9 x 10 -3 mAU/hr, at 254 nm
>2 AU upper limit

Wavelength range
Wavelength accuracy
Slit width
Diode width
Wavelength bunching
Flow cells
Standard
Semi-micro
Micro
Semi-nano
Nano
Preparative
190-950 nm
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
Programmable: 1, 2, 4, 8, 16 nm
< 1 nm
Programmable, 2 - 400 nm, in steps of 1 nm
13-
µl volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum
5µl volume, 6 mm cell path length, 120 bar (1760 psi) pressure maximum
2-
µl volume, 3 mm cell path length, 400 bar (5880 psi) pressure maximum
500-nanoliter volume 10-mm cell path length, 50 bar (735 psi) pressure maximum
80 nanoliter volume., 6-mm cell path length, 50 bar (735 psi) pressure maximum
3-mm cell path length, 120 bar (1760 psi) pressur maximum
0.3-mm cell path lenght, 20 bar (294 psi) pressure maximum, quartz
0.06-mm cell path length, 20 bar (294 psi) pressure maximum, quartz
High pressure
Spectral tools
Analog output
Time programmable
1.7-
µl volume, 6-mm cell path length, 400 bar (5880 psi) pressure maximum
Wavelength, polarity, peak width, lamp bandwidth, autobalance,
WL range, threshold, spectra storage mode
Data analysis software for spectra evaluation, including spectral libraries and peak purity functions
Recorder/integrator 100 mV or 1 V, 2 outputs

Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback messages if preset limits are exceeded. Verification of wavelength accuracy with built-in holmium oxide filter
Add spectral acquisition to your HPLC analyses with the worldwide leading Agilent 1200 Series diode-array detector. Verify separation quality with peak purity, multiple signals and spectral libraries - interactively or automatically in the same run as your analytical quantification. Extended wavelength range from 190 to
950 nm with highest sensitivity or spectral resolutionwith programmable slit. And holmium oxide wavelength verification for
GLP compliance. The temperature management system ensures optimum baseline stability –even under rough ambient conditions.
Access the spectral domain with superior 3D optics – simultaenous illumination using combined deuterium and tungsten lamps for highest intensity and lowest detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest spectral resolution.
Programmable slit helps you explore the spectral landscape for faster sample characterization
– focus on fine bands with narrow setting, or if higher sensitivity is required open up the programmable slit for more light throughput. A broad slit ensures lowest baseline noise and highest chromatographic signal-to-noise.
Store and report slit settings, from 1 - 16 nm, together with your raw data for GLP traceability.
Convenient spectral storage and flexible viewing –easily overlaid spectra within a run or between runs.
Verify wavelength accuracy for GLP compliance – automatic holmium oxide filter (which can be set at the beginning of your chromatography) verifies that your wavelength is indeed what the setpoint says it is.
Connect to LC-MS
– high pressure micro flow cell for extremely small internal volumes are required for LC-
MS applications and use a single Agilent ChemStation to acquire and view UV and MS spectral data.
Explore capillary HPLC – flow rates as low as 5-µl/min with the new 500-nanoliter flow cell. Increase sensitivity 50 to 100-fold compared to standard-bore HPLC.
Low cost of ownership

Graphical Agilent ChemStation user-interface speeds up training time

Long life deuterium lamp (>2000 h)

Built-in diagnostics software
Easy front access to lamp and flow cell for fast replacement