How to conduct behavioral research - lecture notes Steps Involved in Conducting a Behavioral Research Study 1. Formulate a research question 2. Conduct reconnaissance observations 3. Construct an ethogram 4. Choose a sampling method 5. Collect data 6. Analyze data - run statistical analysis 7. Interpret and communicate results 8. Formulate new research question based on results Commonly used resource: "Measuring Behaviour, an introductory guide", 2nd edition authors: Paul Martin and Patrick Bateson Types of Studies Observational o variables not manipulated, behavior correlated with environmental variables, "natural experiments" Experimental o variables are systematically varied by experimenter Reconnaissance Observations what do the animals do time of day best suited recording techniques Ethogram Construction A list of defined behaviors that can be quantified and easily recognized by different observers (observer reliability/repeatability) Objective definitions describe behavior, e.g.., "Locomotion" Functional definitions define behavior in terms of its function, e.g.,“searching, playing" Mutually exclusive (often) Comprehensive (often) Example: Elephant Ethogram Interaction Physical contact between two elephants Walk Quadrupedal locomotion more than 3 ft in previous 5 secs. Run Fast walk Stand still Standing stationary on all four legs, less than 3 ft of movement in previous 5 secs. Eat Consuming food item, includes conveying food item from to mouth Drink Consuming water Explore touch trunk to inanimate object Social Any physical contact between elephants Other describe Out of sight page 1 How to conduct behavioral research - lecture notes Measures of Behavior Latency, Frequency, Duration, Intensity Events & States Bouts Sequence Choosing a Sampling Method Two major decisions to make: Which subject to watch & when o Ad Libitum o Focal o Scan o Behavior Sampling How to record the behavior o Continuous o Time sampling o Instantaneous Point Sampling o One-Zero Recording Medium Film/Video Tape recorder Check Sheets Computer (PC, lap-top, palm) Check Sheet Design Simple Include catch all categories such as: o Out of sight o Other Date, Observer, Weather, Notes Summation columns Data Collection How much to collect? o Variability o Generalisability Duration of observations o Observer fatigue/reliability Inter-Observer reliability page 2 How to conduct behavioral research - lecture notes Sources of Unreliability Inter Observer Reliability - different observers recording different things Intra-Observer Reliability - same observer records behaviors differently over "observer drift" Record what you see (not what you want to see or have seen) Observer fatigue Reliability can be increased by Good, simple ethogram Observer training Observer testing Fewer observers page 3