CURRICULUM VITAE
Peichang Wang
Ph.D
PERSONAL INFORMATION
Sex: Male
Place of Birth: Henan, China
Marital Status: Married
Date of Birth: Jul.28, 1965
Nationality: P.R. China
Health Status: Excellent
CONTACT
Address:
Dept. of Biochemistry & Molecular Biology
Health Science Center, Peking University
Beijing, 100083, P.R. China
E-mail:
changpku@yahoo.com.cn
peichangwang@yahoo.com
Phone Number: 86-10-82801454 (lab.)
EDUCATION AND TRAINING
2001.9-present
Pre-doctoral student, will graduate in July, 2004. Dept. of
Biochem. & Molecular Biology, Health Science Center ,
Peking University, P.R. China
Major in “Molecular Biology of Gerontology”, Supervisor: Prof.
Zong-Yu Zhang & Prof. Tan-Jun Tong.
1993.9-1996.7
Master of Science. Dept. of Biochemistry & Molecular Biology,
Henan Medical University, Zhengzhou, P.R. China.
Major in “Tumor Biology”, Supervisor: Prof. Shu-Heng You.
1983.9-1987.7
Bachelor of Science. Dept. of Biology, East China Normal
University, Shanghai, P.R. China
WORKING AND RESEARCHING EXPERIENCES
2001.9-present
Conducted research on “Natural antioxidants delay the
replicative senescence of human diploid fibroblast (2BS)”.
More than thirty kinds of natural antioxidants , which were
extracted from foods or Chinese herbs, were studied on their
toxic dose for 2BS cells, their potentials of promoting
proliferation & growth of cells, and their effects on the life span
and senescent phenotype of the cells. HDTIC-1 and HDTIC-2,
two compounds from Astragali Radix, were found to meet the
initial conditions of screening. The two compounds were
continually testified by cell cycle analysis, AGEs, comet assay
and the analysis of telomere length. I also observed the effects
of the two compounds on the expression of some
senescence-associated genes (p16INK4a, p21waf1/cip1/sdi1),etc.
1996.7-2001.9
1987.7-1993.9
1993.9-1996.7
Working and teaching in the “ Medical Test Dept., The First
Teaching Hospital of Henan Medical University” as an
advanced technician & associate prof. As the Editor-in-chief, I
compiled the book “Clinical Guide to Clinical Chemistry Test”
(ISBN7-81048-112-6/R, Henan Med. Univ. Pre. Co., 1996.9).
As the major investigator, I finished the researching program
“Study on the antioxidative enzymes in the erythrocytes and
lymphocytes of the workers exposed to cadium” (financially
supported by the Science Fund of Henan Province of China).
Conducted research on “The study of the biomarkers for
esophageal cancer & other diseases”. I found that the activities
of Hexokinase and its isoenzyme Ⅲ increased in the
erythrocytes of the esophageal cancer patients. etc.
EXPERIMENTAL SKILLS
1. Cell Biology
1.1.Cell Culture; Cell Count as well as Cumulative Population Doublings (CPDs);
Phenotype Observation; etc.
1.2. MTT assay; Cell cycle analysis; Single-cell gel electrophoresis; Stain of SA-β
-Gal for cultured cells; etc.
1.3. Light microscopy and fluorescence microscopy, etc.
2. Molecular Biology
2.1.Isolation and purification of genomic DNA, total RNA, total protein and nuclear
protein, etc.
2.2. PCR and PCR-based analysis, such as RT-PCR, TRAP-PCR, etc.
2.3. Nucleic acid hybridization techniques including Southern blot, Northern blot.
2.4. Western blot.
2.5. Fluorescence analysis for advanced glycation end products.
2.6. Gene recombination and gene transfer, etc.
3. Animal Experiment
3.1.i.c.v administration of drugs in mice (ICR mice, balb/c mice).
3.2. Seperation and homogenization of tissues & organs of mice.
3.3. Isolation of cells from mice tissues, etc.
SELECTED PUBLICATIONS
1．Peichang Wang,et al.HDTIC-1 & HDTIC-2, Two Compounds Extracted From
Astragali Radix, Delay Replicative Senescence of Human Diploid Fibroblast.
Mechanism of Ageing & Development,124:1025-1034,2003
2．Peichang Wang,et al.HDTIC-1 & HDTIC-2, Two Compounds Isolated From
Astragali Radix, Slow Down The Shortening Rate of Telomere Via Activation of
Telomerase and Suppression of DNA Damage.(in press).
′
3．Wei-guo Zhu,Yang Ke, Peichang wang, et al.5-aza-2 -deoxycytidine activates
p21/p53 pathway to inhibit cell proliferation. Journal of Biological Chemistry Jan.
13, Epub. Ahead of print, 2004.
4．Peichang Wang, Zongyu Zhang and Tanjun Tong. Genistein improves the growth
and proliferation of 2BS cells & the possible mechanism. Chinese Journal of
Geriatrics. (accepted).( Chinese.)
5．Peichang Wang, Zongyu Zhang and Tanjun Tong. Effects of genistein on the
antioxidative potential and the AGE levels in ICR mice. Chinese Journal of
Gerontology.(revised).(Chinese.)
6. Peichang Wang, et al. Clinical significance of renal epithelial cells detection in
urine of workers exposed to cadmium. Journal of Henan Medical
University,(1):289,2000 (Chinese)
7. Peichang Wang, et al. The determination of GSH-Px activity in erythrocyte of
workers exposed to cadmium. Chinese Journal of Health Laboratory
Technology,10(2):228,2000 (Chinese)
8. Peichang Wang, et al. The measurement of SOD activity in erythrocyte of workers
exposed to cadmium. Occupation & Health,(1):1,2000 (Chinese)
9. Peichang Wang, et al. The effect of antioxidants on GSH-Px activity in
erythrocyte incubated with cadmium. Journal of Industrial Medicine of
china,(2):70,2000 (Chinese)
10. Peichang Wang, et al. The effect of antioxidants on SOD activity in erythrocyte
incubated with cadmium. Journal of Occupational Medicine of china,(2): ,2000
(Chinese)
11. Xianglin Shi, Peichang Wang, et al Vanadium(II) causes
2’-deoxyguanosine hydroxylation and deoxyribonucleic acid Damage Via
free radical reaction. Annals of clinical and Laboratory science, Vol, 26 NO.1
copyright 1996.
12. Peichang Wang, et al. Change of erythrocyte hexokinase and isoenzymes in
esophagus cancer patients prior to and following treatment. Chinese Journal of
clinical Oncology, VOL,23(2):114-116,1996.(chinese)
13. Peichang Wang, et al. Agar-gel electrophoretic method to determine the activities
of hexokinase isoenzymes in erythrocytes and clinical application. Henan
Medical Research, Vol, 5(1):74-76,1996(Chinese)
14. Peichang Wang, et al. Study on the value of sialic acid, Lactic dehydrogenase
and monoamine oxidase from erythrocytes in the diagnosis of esophageal
neoplasma. Henan Journal of Oncology,Vol,8(1):11-12,1995.(Chinese)
15. Peichang Wang, et al. Study on changes of glutathione and bilirubin in the
process of esophageal cancer formation. Journal of Henan
Medical University. VOL, 29(2); 106-108,1994.(Chinese)
MEETING PRESENTATION
1. Peichang Wang. Study of GSH-PX in lymphocytes incubated with cadmium. 87th
Annual Meeting of AOHC , May 12-19, 2000, Philadelphia USA (Oral
Presentation)
2. Peichang Wang. HDTIC-1 & HDTIC-2, Two Compounds Extracted From
Astragali Radix, Delay Replicative Senescence of Human Diploid
Fibroblast.2003 Annual meeting of biochemistry of PKU,Dec.21,
2003, Beijing. (Oral Presentation).
BOOK
Editor-in-chief,[Clinical Guide to Clinical Chemistry Test]
(ISBN7-81048-112-6/R,Henan Medical University Pre.Co.,1996 9,Zhengzhou P.R.
China).
SOCIETY MEMBERSHIPS
Chinese Biochemistry Society; Beijing Biochemistry Society
LANGUAGE AND COMPUTER SKILLS
English: Good in communication and manuscripting.
Computer: Frequent user of SPSS, WORD, EXCEL, POWERPOINT, etc.
REFERENCES
Tan-Jun Tong, MD
Prof. of Biochemistry & Molecular Biology,
Health Science Center, Peking University
38 Xueyuan Rood
Beijing, 100083, P.R. China
Tel: +86-10-8280-1454 (o)
Fax: +86-10-8280-2931
E-mail: ttjzzy@public.gb.com.cn
Wei-guo Zhu, Ph.D
Prof. of Biochemistry & Molecular Biology,
Health Science Center, Peking University
38 Xueyuan Rood
Beijing, 100083, P.R. China
Tel: +86-10-8280-2235 (o)
Fax: +86-10-8280-2235
E-mail: zhuweiguo@bjmu.edu.cn
Zai-Quan Li, Ph.D
Assistant Prof. of Biochemistry & Molecular Biology,
Health & Science Center of Peking University
38 Xueyuan Rood
Beijing, 100083, P.R. China
Tel: +86-10-8280-1631 (o)
Fax: +86-10-8280-1631
E-mail: lizaiquan@bjmu.edu.cn