Supplementary Material
Table 1 References
References:
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Supp. Figure 1: Non-metric Multi-Dimensional Scaling (nMDS) plots of fish tissue C and N
composition (Euclidean Distances based on normalised δ15N, δ13C and C:N). Compositions
did not display any significant differences between seasons (pseudo-F[2,271]=1.49,
p(perm)=0.242) but varied significantly between (a) zones (pseudo-F[5,271]=7.33,
p(perm)<0.01) and (b) trophic groups (pseudo-F[3,271]=6.90, p(perm)<0.05). There was high
correlation (>0.80) between tissue parameters, δ15N (d15N), δ13C (d13C) and C:N, and the
nMDS based on Pearson correlation coefficients (shown with gray lines in b).
Supp. Table 1: The fatty acid (FA) composition (%) of fishes collected during May 2008 differed significantly for carnivores between the Deep reef
slope and Lagoon (pseudo-F[1,6]=80.6, p(perm)<0.05), and for Stegastes fasciolatus and Abudefduf sexfasciatus between the reef slope and reef flat
(pseudo-F[1,8]=2.87, p(perm)<0.05 and pseudo-F[1,4]=7.19, p(perm)<0.05, respectively). ‘Influential’ FAs showing high correlation (>0.90) with
normalised between-sample similarities are shown in bold. Data are mean ± s.e. and the number of samples is shown in parentheses for each zone.
FA
Carnivores
Stegastes fasciolatus
Reef slope
Reef flat
(7)
(3)
Abudefduf sexfasciatus
Reef slope
Reef flat
(2)
(4)
0.00 ± 0.0
0.04 ± 0.0
0.00 ± 0.0
0.00 ± 0.0
0.00 ± 0.0
2.32 ± 0.6
2.21 ± 0.5
0.44 ± 0.1
Deep reef slope
(7)
Lagoon
(4)
0.05 ± 0.1
Saturated
C13:0
C14:0
0.72 ± 0.2
0.69 ± 0.0
0.94 ± 0.2
C15:0
C16:0
0.36 ± 0.1
14.47 ± 3.0
0.51 ± 0.0
14.50 ± 0.4
0.22 ± 0.0
24.69 ± 1.2
0.40 ± 0.1
28.43 ± 1.8
0.21 ± 0.0
28.34 ± 1.9
0.11 ± 0.0
23.61 ± 1.1
C17:0
0.97 ± 0.1
0.98 ± 0.0
0.46 ± 0.0
0.64 ± 0.3
0.22 ± 0.0
0.31 ± 0.0
8.51 ± 0.6
0.29 ± 0.0
6.66 ± 0.9
9.38 ± 0.2
0.31 ± 0.0
0.36 ± 0.0
C18:0
7.33 ± 2.3
8.90 ± 2.0
C19:0
0.49 ± 0.1
0.33 ± 0.0
8.96 ± 0.5
0.33 ± 0.0
C20:0
0.25 ± 0.0
0.32 ± 0.0
0.29 ± 0.0
0.31 ± 0.1
0.20 ± 0.0
0.36 ± 0.0
C21:0
0.51 ± 0.2
0.34 ± 0.2
0.04 ± 0.0
0.08 ± 0.0
0.00 ± 0.0
0.04 ± 0.0
0.52 ± 0.2
0.06 ± 0.0
0.00 ± 0.0
0.11 ± 0.0
0.00 ± 0.0
0.05 ± 0.0
C22:0
0.34 ± 0.1
0.41 ± 0.0
C23:0
0.40 ± 0.2
0.65 ± 0.1
0.16 ± 0.1
0.05 ± 0.0
C24:0
0.32 ± 0.1
0.19 ± 0.0
0.27 ± 0.0
0.19 ± 0.0
0.13 ± 0.0
0.20 ± 0.0
i-C15:0
0.39 ± 0.1
0.16 ± 0.0
0.04 ± 0.0
0.07 ± 0.0
0.00 ± 0.0
0.00 ± 0.0
i-C16:0
0.28 ± 0.1
1.26 ± 0.3
0.05 ± 0.0
0.05 ± 0.0
0.03 ± 0.0
0.00 ± 0.0
i-C17:0
0.39 ± 0.2
1.40 ± 0.4
0.35 ± 0.0
0.27 ± 0.1
0.63 ± 0.2
0.29 ± 0.0
i-C18:0
0.65 ± 0.2
2.53 ± 0.6
0.39 ± 0.0
0.33 ± 0.0
0.65 ± 0.3
0.29 ± 0.0
1.30 ± 0.3
1.93 ± 0.2
2.02 ± 0.4
3.86 ± 0.3
9.04 ± 4.0
1.94 ± 0.2
Branch-saturated
Monounsaturated
C16:1 n-7
Carnivores
Deep reef slope
(7)
Lagoon
(4)
i-C17:1
0.75 ± 0.1
0.37 ± 0.2
Stegastes fasciolatus
Reef slope
Reef flat
(7)
(3)
0.13 ± 0.0
0.09 ± 0.0
C17:1 n-7
1.06 ± 0.2
0.66 ± 0.1
0.64 ± 0.1
0.32 ± 0.1
C18:1 n-12
3.37 ± 1.5
0.34 ± 1.3
0.22 ± 0.0
0.53 ± 0.1
0.20 ± 0.1
0.00 ± 0.0
3.09 ± 0.7
10.19 ± 1.8
1.99 ± 0.2
1.06 ± 0.2
1.53 ± 0.1
C18:1 n-9c
1.53 ± 0.2
5.22 ± 2.4
1.77 ± 0.1
6.80 ± 0.6
6.87 ± 0.6
9.11 ± 1.3
5.61 ± 0.2
C20:1 n-11
0.16 ± 0.1
0.08 ± 0.0
C20:1 n-7
0.08 ± 0.1
0.30 ± 0.2
0.49 ± 0.1
0.00 ± 0.0
0.40 ± 0.3
0.00 ± 0.0
1.22 ± 0.1
0.00 ± 0.0
0.03 ± 0.0
0.16 ± 0.1
C20:1 n-9
0.58 ± 0.1
0.65 ± 0.1
0.36 ± 0.1
0.65 ± 0.2
0.46 ± 0.1
0.88 ± 0.0
C24:1 n-9
0.31 ± 0.1
0.17 ± 0.0
0.18 ± 0.0
0.30 ± 0.0
0.09 ± 0.1
0.23 ± 0.0
0.11 ± 0.2
0.68 ± 0.2
0.07 ± 0.0
0.41 ± 0.1
0.37 ± 0.1
0.34 ± 0.0
0.27 ± 0.2
0.14 ± 0.1
1.24 ± 0.0
0.00 ± 0.0
1.28 ± 0.1
0.13 ± 0.0
0.16 ± 0.0
FA
C18:1 n-7
Abudefduf sexfasciatus
Reef slope
Reef flat
(2)
(4)
0.00 ± 0.0
0.19 ± 0.0
0.16 ± 0.0
0.21 ± 0.0
Polyunsaturated
C16:2n-4
C16:4n-3
0.51 ± 0.2
0.55 ± 0.2
0.07 ± 0.0
C18:2n-6c
0.72 ± 0.1
1.20 ± 0.2
C18:2n-6t
0.35 ± 0.1
0.39 ± 0.1
0.82 ± 0.0
0.14 ± 0.0
1.11 ± 0.2
0.11 ± 0.0
C18:3 n-3c
0.21 ± 0.0
0.41 ± 0.1
0.35 ± 0.1
0.67 ± 0.4
0.40 ± 0.1
0.16 ± 0.0
0.00 ± 0.0
0.00 ± 0.0
0.00 ± 0.0
C18:3 n-6
0.23 ± 0.1
0.14 ± 0.2
0.00 ± 0.0
C18:3 n-3c
0.28 ± 0.1
0.27 ± 0.0
C18:4 n-3
0.31 ± 0.2
0.32 ± 0.0
1.03 ± 0.3
0.03 ± 0.0
1.58 ± 0.3
0.34 ± 0.3
4.32 ± 0.6
0.15 ± 0.1
2.33 ± 0.1
0.22 ± 0.0
C18:4 n-5
0.03 ± 0.0
0.08 ± 0.0
0.05 ± 0.0
0.07 ± 0.1
0.00 ± 0.0
0.12 ± 0.0
0.21 ± 0.0
0.27 ± 0.0
0.14 ± 0.1
1.29 ± 0.0
0.20 ± 0.0
C20:2 c-11,14
0.32 ± 0.0
0.48 ± 0.1
0.22 ± 0.0
C20:3 isomer
0.20 ± 0.0
0.32 ± 0.0
1.25 ± 0.2
0.95 ± 0.0
C20:3 n-3
0.42 ± 0.1
0.35 ± 0.0
0.56 ± 0.1
0.72 ± 0.1
0.96 ± 0.0
0.24 ± 0.0
C20:4 n-6
5.22 ± 1.4
9.89 ± 1.3
11.23 ± 1.5
9.89 ± 0.3
3.75 ± 0.4
7.20 ± 0.3
0.45 ± 0.1
6.01 ± 0.3
0.04 ± 0.0
0.00 ± 0.0
0.27 ± 0.0
C20:5 n-3*
0.52 ± 0.2
2.57 ± 0.1
0.23 ± 0.1
C22:2
1.40 ± 0.2
3.36 ± 0.7
4.91 ± 0.7
1.25 ± 0.1
8.81 ± 2.3
3.04 ± 1.0
3.05 ± 0.4
0.08 ± 0.0
3.54 ± 0.0
0.08 ± 0.0
C20:4 n-8
Carnivores
Deep reef slope
(7)
Lagoon
(4)
C22:4 n-6
0.66 ± 0.5
0.14 ± 0.2
Stegastes fasciolatus
Reef slope
Reef flat
(7)
(3)
0.12 ± 0.0
0.18 ± 0.1
C22:5 n-3
2.15 ± 0.1
4.24 ± 0.9
4.96 ± 0.5
4.32 ± 0.5
3.09 ± 0.3
4.40 ± 0.2
C22:6 n-3
24.13 ± 5.5
10.93 ± 4.6
14.67 ± 1.5
4.70 ± 1.7
12.68 ± 5.4
19.62 ± 0.7
FA
Abudefduf sexfasciatus
Reef slope
Reef flat
(2)
(4)
0.93 ± 0.1
1.91 ± 0.1