Biogeochemical Cycles Adapted by Carrie Jones and Jamie Church for 9-12 Marine Science class A. Standards (Ocean Literacy standards) 1. The ocean has one big ocean with many features 2. The ocean and life in the ocean shape the features of the Earth 4. The ocean makes Earth habitable 5. The ocean supports a great diversity of life and ecosystems 6. The ocean and humans are inextricably connected B. Performance Objectives Following completion of this lesson, students will be able to: Understand how nutrient cycles throughout the oceans Understand that nutrients are essential for life Apply nutrient cycling to a closed ecosystem and how it will affect the living things in it Journal about the progress of their ecosystem Assess how their ecosystem survived by concepts of nutrient cycling C. Lesson Plan 1. Concept Exploration Set-up stations around the room or outside representing different areas of an ecosystem where nitrogen (or you may choose a different nutrient) enters or exits an area. We used atmosphere, surface water, rain water, groundwater, fertilizer, soils, ocean, live animals, dead plants and animals, animal waste and live plants as our stations. You will need 11 dice and a stamp or different colored marker for each station. Introduce nitrogen, where it is found and why it is important. Explain that the students are playing the role of a nitrogen atom and they are traveling through a nitrogen cycle based on dice rolls. They will mark on a passport where they traveled (see attached worksheet). Afterwards, ask them to diagram how they traveled. http://www.windows.ucar.edu/teacher_resources/nitrogen_activity.pdf 2. Concept Introduction Nitrogen is part of the biogeochemical cycles. Most of the nitrogen is in the atmosphere and all living things need nitrogen. Although the nitrogen cycle is very complex, it is probably the most important nutrient cycle because it is usually the most limiting factor for plants, is very soluble and is the most mobile (http://www.ag.ohio-state.edu/~prec/soil/n_cycle.htm). Nitrogen is transformed through nitrification, denitrification, mineralization and fixation by bacteria. When in the form of ammonia, it is highly toxic to fish. Excess of nutrients can lead to contamination and/or eutrophication. Many human influences have affected this cycle and thus the organisms in the water where it is concentrated and needed for organisms to grow are highly affected. Explain that when setting up an aquarium, you must establish the bacteria and nutrients before putting the animals in it. There are different types of filtration systems that contain the bacteria needed. You also need a clean hard surface for the bacteria to live on. 3. Concept Application Students will apply what they learned about nutrient cycling and aquariums to set-up their own closed ecosystem. Purchase or collect a hardy freshwater fish. We used guppies since they are cheap, easy to get from any pet store, are sexually dimorphic, have short gestation periods and give live birth. This also gives you the opportunity to explain concepts of ecology and reproduction. You will need some type of container with a lid such as a pickle jar or large plastic pretzel jar, freshwater plants, gravel and sand. Provide pond water and they will choose if they want to use tap, pond or filter the pond water with a coffee filter. The students should get in groups of 2-3 students. A couple days before you bring in the guppies, they will need to set up their jars. This is an inquiry-based project. The students will decide how to set-up their jars and how much of what materials they will use. Give them a rationale sheet asking what substrate they used (gravel or sand), how much and why, how many plants and what type of water (tap, pond or filtered pond) and why. The students can use a mixture of any or all materials, let them decide! They must leave the lids off after they set-up their jars so the gases may dissolve out if they used tap water. They also need to choose a location in the room. Explain if they put it by a window, the plants may survive better, however the biggest stressor for fish is temperature change. After their ecosystems have had a few days to establish, they will put the guppies in, 4-5 guppies per group (1 male, 4 females) and close the lid. The most important thing is to not open the lid until the experiment is done. Everyday, the students will make close observations and record them in a journal for a couple of weeks. They should record behaviors of the fish, health of the plants, if any babies are born, if the fish are seen eating the plants and if the fish are near the top, bottom, etc. D. Review/Evaluation Assess the students understanding through having them write a paper explaining how their closed ecosystem sustained itself through nutrient cycling. They need to include terms such as nitrifying bacteria, photosynthesis, decomposition and consumption. They should hypothesize if they think their substrate, plants, placement or what type of water the used contributed to the success/failure of their ecosystem, animals and if they had any babies. Sources: http://www.windows.ucar.edu/teacher_resources/nitrogen_activity.pdf http://www.ag.ohio-state.edu/~prec/soil/n_cycle.htm