1
Supporting Information
Evaluation of a curcumin analog as an anti-cancer agent inducing ER stress-mediated
apoptosis in non-small cell lung cancer cells
Yi Wang1,#, Zhiguo Liu1,#, Xiuhua Zhang2, Luqing Ren1, Yi Huang1, Yuepiao Cai1, Xueqian Shen1,
Xiaokun Li1, *, Guang Liang1, *
1
Chemical Biology Research Center, School of Pharmacy, Wenzhou Medical University, University
Town, Wenzhou, Zhejiang, China
2
Department of Pharmacy, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou,
Zhejiang, China
Table S1. Chemical information and sources of the tested 113 compounds
Comp.
Structure
Ref.
Comp.
A67
1
A68
2
A69
3
[1]
Structure
Ref.
[3]
A70
4
A72
5
A73
6
2
A74
7
A75
8
B67
9
B68
10
B69
11
B72
12
B73
13
B74
14
B75
15
C67
16
C68
17
C69
18
C70
19
3
C72
20
21
C73
C74
22
C75
23
A76
24
A77
25
A78
26
A80
27
A81
A82
A84
A86
[2]
28
29
30
31
4
32
A88
33
NA1
34
NA2
35
NA3
36
NA4
37
B76
38
B78
B82
39
40
B84
[2]
B86
NB1
41
42
5
43
NB2
NB3
NB4
C77
C78
C80
C81
C82
C84
C86
C87
44
45
46
47
48
49
50
51
52
53
6
54
NC1
NC2
C02
[4]
NC3
NC4
Figure S1. B82 treatment increased the expression of cleaved-PARP, P53, and Bax. Method: H460 cells
were treated with B82 at the indicated concentrations for 24 h, and then were harvested for protein
extraction. The protein levels of cleaved-PARP, P53, and Bax were detected by western blot analysis using
antibodies from Santa Cruz Biotechnology (Santa Cruz, CA). Representative western blots were shown
from three independent experiments.
References:
1.
Zhao C, Yang J, Wang Y, Liang D, Yang X, Li X, Wu J, Wu X, Yang S, Liang G: Synthesis of
mono-carbonyl analogues of curcumin and their effects on inhibition of cytokine release in
LPS-stimulated RAW 264.7 macrophages. BioorgMedChem 2010.
2.
Zhao C, Cai Y, He X, Li J, Zhang L, Wu J, Zhao Y, Yang S, Li X, Li W et al: Synthesis and
anti-inflammatory evaluation of novel mono-carbonyl analogues of curcumin in LPS-stimulated
RAW 264.7 macrophages. Eur J Med Chem 2010, 45(12):5773-5780.
3.
Wu J, Li J, Cai Y, Pan Y, Ye F, Zhang Y, Zhao Y, Yang S, Li X, Liang G: Evaluation and discovery of
novel synthetic chalcone derivatives as anti-inflammatory agents. J Med Chem 2011,
54(23):8110-8123.
4.
Liang G, Li X, Chen L, Yang S, Wu X, Studer E, Gurley E, Hylemon PB, Ye F, Li Y et al: Synthesis
and anti-inflammatory activities of mono-carbonyl analogues of curcumin. Bioorg Med Chem Lett
2008, 18(4):1525-1529.