Potassium permanganate, solid 200mM Potassium permanganate Hazardous Substances Policy - Assessment CHEMICAL HAZARD AND RISK ASSESSMENT School of Biosciences Name of supervisor Dr S. Minchin Assessor Rita Godfrey Assessment Number* 1417 Date of Assessment 12 / 6 / 01 Signature Signature Notes A School COSHH form in Word is available on the School Server. Available from the Health and Safety Unit. Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment. Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form. Use a continuation sheet to expand any section of this form in hard copy version. 1 LOCATION OF THE WORK ACTIVITY 2 PERSONS WHO MAY BE AT RISK List names where possible All occupants of the lab (Laura Tate, Jo Field, Dong Ling Zeng, Rita Godfrey) 3 ACTIVITY ASSESSED 4 MATERIALS INVOLVED NAME Potassium permanganate, solid Lab G10 Potassium permanganate footprinting AMOUNT max/stored 500g Attach copies of data sheet(s) HAZARD OXIDIZING HARMFUL DANGEROU S FOR THE ENVIRONMENT 200mM Potassium permanganate 5ml DANGEROU S FOR THE ENVIRONMENT DNA sample ~100ug HAZARD GROUP 1 IN GMAG RULES Protein sample 4ml NSH 5x Binding buffer: 25% Glycerol 0.5M NaCl 25mM MgCl2 0.5mM EDTA 5mM DTT 0.25mg/ml BSA 100mM Tris-HCl pH 8.0 10ml IRRITANT RISK PHRASES R8 Contact with combustible material may cause fire R22 Harmful if swallowed R50/53 Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment R51/53 Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. R36 Irritating to eyes. HAZDAT NO*** 1033 BIOSCIENCESNO*** Permanganate Stop: 3M Ammonium acetate 0.1M EDTA 1.5M 2-mercaptoethanol 100ml Phenol/Chloroform/Isoamyl alcohol 25:24:1 100ml HARMFUL IRRITANT TOXIC CORROSIVE HARMFUL Category 3 carcinogen R21 Harmful in contact with skin R36/37/38 Irritating to eyes, respiratory system and skin R24/25 Toxic on contact with skin and if swallowed. R34 Causes burns R40 Possible risk of irreversible effects. R48/20/22 Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed 100% Ethanol 2.5 litres HIGHLY FLAMMABLE R11 Highly flammable 80% Ethanol 50ml HIGHLY FLAMMABLE R11 Highly flammable Glycogen 20mg/ml 1ml NSH Sterile distilled water 50ml NSH TE buffer 100ml NSH 5 571 176 and 44 INTENDED USE** Give brief details and attach protocol/instructions Potassium permanganate footprinting, see attached sheet. This assessment covers only the DNA modification by permanganate, up to step 12 on the method sheet, and it does not cover the labelling of the primer (assessment number 1401) The next stage, PCR primer extension is covered in assessment number1397 6 RISKS to HEALTH and SAFETY from INTENDED USE From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk? There is a risk of contact with the solid potassium permanganate as well as the solution. There is a fire risk from the ethanol There is a risk of exposure to phenol/chloroform vapours and also skin contact with it. There is a risk of skin contact and inhalation of the KmnO4 Stop solution 7 CONCLUSIONS ABOUT RISKS Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? Control measures are necessary as the risks are significant, although the risk from the 200mM KMnO4 solution is very slight because of the small volumes used. 8 CONTROL MEASURES Additional to Good Chemical Practice Wear protective clothing and gloves when weighing out KMn04 . Wear protective clothing, gloves and goggles or a face mask when working with phenol/chloroform. Replace caps and bottle tops as soon as possible and avoid inhaling phenol/chloroform vapours. See assessment number 1360 for more details on working with phenol/chloroform. Have PEG 300 at hand in case of accidents. Transfer of waste phenol/chloroform to the halogenated waste solvent bottle should be done in the fume hood Wear gloves when handling the KMnO4 Stop solution Do not allow solutions or spillages of KMnO4 to dry out 9 INSTRUCTION/TRAINING Specify course(s) and/or special arrangements. 10 MONITORING Performance of control measures, Check integrity of gloves before starting. Check fume hood is working. Personal exposure Health Surveillance 11 WASTE DISPOSAL PROCEDURE See School Server for Approved Procedure Document on specific Chemical Waste Disposal. Waste ethanol containing solutions should be placed in the non-halogenated waste solvent bottle, which is taken to Stores when full. The waste phenol/chloroform should be transferred to the halogenated waste solvent bottle. This should be done in the fume hood. Waste 200mM KMnO4 can be washed down the sink after diluting it greatly with water first. Only small amounts of this can be disposed of this way. Large amounts would have to be taken down to Stores for specialist disposal. Waste Stop solution can also be poured down the sink with running water. 12 REVIEW Enter the date or circumstances for review of assessment (maximum review interval 5 years) June 2006 13 EMERGENCY ACTION TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. Switch off all sources of ignition and contain spillage with paper towels if it is small or use the spill kit if it is large. Spillage of solid KMnO4 should be scooped up into a leakproof container, labelled and taken to Stores for specialist disposal. Do not allow spillages of KMnO4 solution to dry out. Mop up straight away and wash paper towels with plenty of water so that the KMnO4 does not dry out on the towels instead. TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid Protective clothing, gloves and goggles should be worn. For ethanol, phenol/chloroform and KMnO4 Stop solution the room should be ventilated until all vapours have gone. For phenol/chloroform the room should be evacuated in the event of a spillage. TO RENDER SITE OF EMERGENCY SAFE Clean-up/decontamination The site of a spillage of KMnO4 solid should be washed with plenty of water All other sites should also be washed with water . CONTACT Dr S. Minchin PHONE 45438 10.10.00 * ** *** Prefix T is used for Teaching Assessment Number. Please include amount of chemicals used and how. Hazdat No is the UNICOSHH datasheet report number. Biosciences No is the Biosciences data sheet number. UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER. BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.