“A. TSAKALIDIS Inc.”
PHYSICAL AND CHEMICAL TESTING LABORATORY
12, TSAMADOU STREET
PIRAEUS – GR 18531
TEL. +302104175865
FAX. +302104172895
e-mail : gerg@hol.gr
LEGAL REPRESENTATIVE: A. TSAKALIDIS
1. ORGANIZATION
The laboratory was founded in 1940 by the chemist Th. Papasilekas and since 1986, it
operates as an incorporation company under the name “Antonios Tsakalidis Inc.” The laboratory is
located in Piraeus and it employs a staff of 20 scientists and technicians. Its installations currently
occupy a total space of 450 m2, spanned to 4 building floors.
The laboratory’s activities include the conduct of chemical, physical and microbiological
testing and analysis of water, food, pharmaceuticals and environmental samples. It is accredited
according to ISO 17025 by the Hellenic Accreditation Council and recognized by the Hellenic
Ministry of Agriculture and the Uniform Body for Food Control. It is an active member of the
following organizations:

HellasLab, National Member of Eurolab

Union International de Laboratoire Independants

Association of Official Analytical Chemists

International Technical Division on Reference Materials
The laboratory's organization chart is the following :
GENERAL DIRECTOR
EXTERNAL
EXPERT
CONSULTANTS
DIRECTOR OF
TECHNICAL AND
CONSULTING
SERVICES
OFFICE OF
SAMPLE
RECEPTION
AND
HANDLING
DEPARTMENT
OF CUSTOMER
SERVICE AND
CONSULTING
DEPARTMENT
OF CLASSIC
ANALYSIS
DIRECTOR OF
QUALITY
MANAGEMENT
OFFICE OF
INTERNAL
AUDITING AND
EVALUATION
DEPARTMENT
OF FOOD AND
BEVERAGES
ANALYSIS
DIRECTOR OF
FINANCE AND
PLANNING
SUPPLIES
OFFICE
DEPARTMENT OF
ENVIRONMENTAL
ANALYSIS
ACCOUNTING
OFFICE
DEPARTMENT OF
MICROBIOLOGICAL
ANALYSIS
2. STAFF
The laboratory employs a number of scientists, technicians and engineers, making a total of
20 employees. All employees are university graduates with adequate working experience to
accomplish their duties. The laboratory’s quality system includes specialized training as well as
periodical evaluations of personnel efficiency. Furthermore, the staff members participate in
seminars and conferences according to their area of expertise.
3. INSTRUMENTATION
The laboratory’s instrumentation covers a wide range of analytical techniques very well
suited for food and environmental analysis. For each instrument a detailed history logbook is kept,
in which all analysis and maintenance data are recorded. All instruments are inspected periodically
by authorized service personnel.
The analytical techniques include the following :
Gas Chromatography
 Gas Chromatograph / Mass Spectrometer
 Gas Chromatograph / FID / ECD
 Gas Chromatograph / NPD / FPD
(Agilent)
(Agilent)
(Agilent)
Liquid Chromatography
 Liquid Chromatograph / Diode Array Detector
 Liquid Chromatograph / Post column derivatisation / Fluorescence Detector
 Liquid Chromatograph / Refracting Index Detector
 Liquid Chromatograph / Conductivity Detector
(Agilent)
(Waters)
(Varian)
(Varian)
Atomic Absorption Spectrometry
 Atomic Absorption Spectrometer / Flamme / Cold Vapour
(Varian AA 280 FS/VGA-77)
 Atomic Absorption Spectrometer / Graphite Furnace / Zeeman (Varian AA 240Z)
 Atomic Absorption Spectrometer / Graphite Furnace
(Perkin Elmer 2380 / HGA 400)
Spectrometry
 Spectrometer UV-VIS
 Spectrometer FT IR
 Spectrometer ELISA
(Hitachi U-2000)
(Foss Electric FT 120)
(Hyperion)
Electrophoresis
 Electrophoresis vertical
 Electrophoresis IEF
(Hoefer)
(LKB)
General Instrumentation
 Automated Soxtec
 Automated Kjeldahl
 Automated Kjeldahl
(Tecator)
(Buchi)
(Velp)
Microbiological Instrumentation
4. QUALITY MANAGEMENT SYSTEM
The laboratory employs the quality system that is dictated by ISO 17025 standard, whose
specifications include all the specifications of the ISO 9000 standard and is accredited by the
Hellenic Accreditation Council for 37 chemical and microbiological analytical tests. The laboratory
also follows a specific waste disposal procedure for its used solvents and reagents by cooperation
with waste disposal agencies.
5. SERVICES
5.1 FOODS
 Nutritional labeling. Proteins, fats (saturated, mono-poly-unsaturated, trans, ω3, ω6),
carbohydrates, sugars, sugar alcohols, Dietary Fibers, moisture, Ash, Sodium, Calcium,
Potassium, Calories, Vitamin Α and C, Cholesterol.
 Vitamins
 Food Additives & Preservatives. (Sorbic, Benzoic, Propionic, Boric acid, Sulfites e.t.c.)
 Heavy metals
 Natural Toxins. Aflatoxins (B1, B2, G1, G2), Ochratoxin Α, DON, Zearalenone, Patulin.
 Pesticides Residues (Chlorinated, Nitrogen, Organophosphates and Triazines)
 PAH’s
 PCB’s
 Meat species differentiation
 Bovine milk detection in sheep milk products
 Oils & Fats analysis
 Wine analysis
 Panel test
 Microbiological analysis. Salmonella, Listeria monocytogenes, Staphylococcus coagulase
positive, Total coliforms, E. coli, Enterobacteriacea, Bacillus cereus, Clostridium perfigens,
Legionella.
5.2 REGISTRATION AND DECONTROL OF PRODUCTS
5.3 WATER
 Chemical and microbiological analysis according to directive 98/83 E.U.
5.4 ENVIRONMENTAL
 Volatile Organic Compounds (VOC’s)
 Semivolatile Organic Compounds
 BTEX
 Phenols
 PAH’s
 PCB’s
 Pesticides Residues (Chlorinated, Nitrogen, Organophosphates and Triazines)
 Heavy metals
 Total Petroleum Hydrocarbons (G.R.O., D.R.O.)
 Tensides
6. EXPERIENCE
6.1 EXPERIENCE IN FOOD ANALYSIS
The laboratory's activities and accreditation in the analysis of several parameters, have produced an
extensive experience in analysis of different type of food The parameters analyzed range from
nutritional labeling and microbiological safety to heavy metals, organic pollutants like PAHs,
Toxins and pesticides.
6.2 EXPERIENCE IN ENVIRONMENTAL ANALYSIS
The laboratory's activities and accreditation in the analysis of several parameters, have produced an
extensive experience in environmental analysis. The parameters analyzed range from physical
properties and metals to organic pollutants like PAHs, PCBs, VOCs, BTEX and pesticides.
The Clients of the laboratory are Municipalities, Government services, Industries, Environmental
Management firms, Private individuals and Public Organizations. The services offered include
water, wastewater and soil analysis from various sources.
6.3 INTERLABORATORY TESTS
Participating in international interlaboratory tests assesses the quality and objectivity of
results. The laboratory participates successfully in the following interlaboratory testing schemes:
Organizer: N.W.R.I. – (Canada)
Subjects:
Major Ions & Nutrients
Trace elements and heavy metals
Mercury
Organizer: Hellenic Accreditation Council (GR)
Subject:
Natural Mineral Water
Organizer: Institute for Reference Materials and Measurements (European Commission)
Subject:
Trace Elements in Water
Organizer: Public Health Laboratory Service
Subject:
Food microbiology
(UK)
Organizer: American Oil Chemists’ Society (USA)
Subjects:
Nutritional Labeling
Fish Meal
Olive Oil
Oilseed Meal
Gas Chromatography
Aflatoxin-Cottonseed Meal
Organizer: Public Health Laboratory Service, National School of Public Health (UK)
Subject:
Water microbiology
Organizer: C.E.C.A.LAIT (France)
Subject:
Soft Cheese