Auxiliary Materials TREE-RING-BASED SEVEN CENTURY DROUGHT RECORDS FOR THE WESTERN HIMALAYA, INDIA Ram R Yadav Birbal Sahni Institute of Palaeobotany 53, University Road Lucknow 226007, India Phone: 091-522-2742907 Mobile: 09450394957 Fax: 091-522-2740485 Email: rryadav2000@gmail.com 1. Data and Methods 1.1 Tree-Ring Data [1] The increment core samples from Himalayan cedar (Cedrus deodara (Roxb.) G. Don) and neoz pine (Pinus gerardiana Wall ex. Lamb) were collected from nine and two moisture stressed sites respectively in Kinnaur, a cold arid region in Himachal Pradesh, the western Himalaya. The tree core samples collected in summer 2005, 2006 and 2011 from ecologically homogeneous sites were used together to develop large chronology network for this area. Undisturbed trees of different age classes without any visible sign of lopping or fire were selected for sampling using an increment borer. The increment core samples were air dried and fixed on wooden support using glue so that the cross surfaces face atop. The cross surface of the mounted increment core samples were polished with different grades of abrasives to make the cellular details of growth rings discernible under the binocular microscope. The ring width sequences among samples were precisely dated to the exact calendar year by using pattern matching within a tree and between trees from a site and different sites using skeleton plotting [Stokes and Smiley, 1968]. The ring widths in dated samples were measured using the LINTAB measuring system with a resolution of ±0.01 mm. The dating of growth ring sequences were crosschecked using COFECHA, a dating quality check program [Holmes, 1983] and ring width plots [Rinn, 1991]. The tree-ring width chronologies of Himalayan cedar (nine sites) 1 and neoza pine (two sites) were developed using the program ARSTAN [Cook, 1985]. For detrending of measurement series conservative method of cubic smoothing spline with a 50% frequency response cut off width equal to 2/3rd of the individual series length was employed. However, before detrending, an adaptive power transformation was applied to stabilize the variance in heteroscedastic tree-ring width measurement series [Cook and Peters, 1997]. The growth trends were removed from the power transformed individual measurement series by subtraction, which minimizes the end fitting-type bias as compared to the ratios. All detrended series of respective sites were averaged to a mean chronology by computing the biweight robust mean in order to reduce the influence of outliers. The population signal in the mean chronology gradually weakens in early part of the chronology due to decline in sample replication. The expressed population signal (EPS) of 0.85, considered to be a reasonable threshold for acceptance of chronology quality [Wigley et al., 1984] in dendroclimatic studies, was taken into account to truncate the chronologies for further studies. The site and species chronology statistics and chronology plots with expressed population signal (eps) are shown in tale S1 and Figure S1. All the site and species chronologies showed strong common signal as measured by cross-correlation analysis for the common period AD 1660-2005 (table S2). 2 Table S1. Site chronology statistics. The details of site locations are shown in figure 1. EPSexpressed population signal, MI-mean index, MS-mean sensitivity, SD-standard deviation, AR1-first order autocorrelation. 1 Akpa* 31º35´ N-78º 23´E 1355-2010 41/30 Ist Year with EPS >0.85 1435 2 Jangi* 31º36´ N-78º 25´ E 1353-2005 28/18 1535 0.995 0.397 0.404 0.452 3 Katgaon* 31º32´ N-78º 16´ E 1480-2005 81/53 1540 0.987 0.399 0.377 0.341 4 Kilba* 31º31´ N-78º 08´ E 1432-2005 32/22 1515 1.004 0.375 0.371 0.388 5 Nichar* 31º33´ N-77º 58´E 1580-2005 47/32 1640 0.998 0.335 0.328 0.336 6 Purbani* 31º35´ N-78º 18´ E 1257-2005 55/36 1310 0.990 0.395 0.340 0.235 7 Ralli* 31º34´ N-78º 23´ E 1376-2005 51/39 1430 0.982 0.458 0.395 0.225 8 Roghi* 31º32´ N-78º 17´ E 1388-2005 61/45 1440 0.989 0.418 0.378 0.304 9 Sangla* 31º25´ N-78º 16´ E 1607-2005 24/19 1660 1.000 0.362 0.362 0.408 10 Akpa** 31º35´ N-78º 23´ E 1203-2010 91/77 1285 0.992 0.486 0.389 0.113 11 Purbani** 31º35´ N-78º 18´ E 919-2005 35/30 1495 0.980 0.552 0.457 0.231 S. No. Site Location Chronology length (A.D.) Cores/ trees MI MS SD AR1 0.996 0.400 0.371 0.342 * Himalayan cedar, ** Neoza pine Figure S1. (a) Standard version of the Himalayan cedar chronologies (1-9) and neoza pine (10-11), (b) change in eps level over the length of chronologies. The eps values were calculated over 50-years period with the slide of 25 years. The numbers indicate sites as in table 1; Himalayan cedar (1-9) and neoza pine (10-11). 3 Table S2: Correlation among the site and species chronologies [1660-2005]; Details of sites are the same as specified in Table S1. Sites/ species 2* 3* 4* 5* 6* 7* 8* 1* 2* 3* 4* 5* 6* 7* 8* 9* 10** .904 .818 .789 .819 .796 .927 .697 .669 .911 .863 .925 .875 .865 .884 .766 .856 .872 .861 .859 .769 .908 .851 .853 .893 .909 .831 .907 .904 .855 .864 .874 .896 .825 .814 .736 .927 .865 .860 .800 9* .813 .836 .828 .815 .745 10** .911 .853 .764 .787 .672 11** .858 .830 .818 .860 .728 * Himalayan cedar, ** Neoza pine .893 1.2 Climate data The precipitation data of five weather stations in Satluj valley close to the sampling sites in Kinnaur, Himachal Pradesh were used in this study (Table S3) for tree-growth/climate relationship. Strong coherence in precipitation data of these stations as measured by crosscorrelation was indicated (Table S4). Table S3. Location of meteorological stations and length of records Station Length Kilba Altitude (m) 1901-2000 2480 Position 31º 31´ N - 78º 09´ E Nichar 1930-2001 2322 31º 35´ N - 77º 57´ E Kalpa 1951-2004 2756 31º 32´ N - 78º 15´ E Sangla 1951-2001 2674 31º 25´ N - 78º 15´ E Purbani 1951-2004 2964 31º 35´ N - 78º 21´ E 4 Table S4. Correlation between annual (January-December) precipitation of different stations used in present study (two tailed p values are given in brackets). The correlations were calculated for the common period 1951-1995. Station Kilba Nichar Kalpa 0.553 (0.0001) Nichar Sangla Purbani 0.632 (0.0001) 0.748(0. 0001) 0.801 (0.0001) 0.539 (0.0001) 0.462 (0.0014) 0.422 (0.0039) 0.444 (0.0022) 0.528 (0.0002) 0.767 (0.0001) kalpa Sangla REFERENCES Cook, E. R. (1985), A time series approach to tree-ring standardization, Ph.D. thesis, Univ. of Ariz., Tucson, 171 pp. Cook, E. R., and K. 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