CURRICULUM VITAE -PARASKEVI MOUTSATSOU
EDUCATION AND QUALIFICATION
Bachelor in Chemistry , Aristotelian University of Thessaloniki, Greece
Μ.Sc. in Steroid Endocrinology , Department of Chemical Pathology, Medical School, University of
Leeds, U.K.
PhD in Steroid Endocrinology , Department of Chemical Pathology, Medical School, University of
Leeds, U.K.
Speciality in Clinical Chemistry , Greek Ministry of Health
TEACHING in undergraduate courses:

Medical Chemistry ( Medical and Dental School )

Biochemistry ( Medical and Dental School and School of Nursing )

Clinical Biochemistry ( elective course/Medical School and School of Nursing )

Physiology of Bone Tissue ( elective course/Medical School )
TEACHING (Biochemistry, Clinical Biochemistry) in postgraduate courses offered by the Medical School, Department of Chemistry and Department of Biology of the University of Athens leading to a Master of Science (MSc.) degree :
 “Molecular Medicine” MSc. Course ( Medical School )
 “Metabolic Disorders of Bone Tissue” MSc. Course ( Medical School )
 “Molecular and Applied Physiology” MSc. Course (
Medical School )
 “Laboratory and Clinical Medicine” MSc. Course ( Medical School )
 “Clinical Chemistry” MSc. Course ( Dept of Chemistry )
 “Clinical Biochemistry-Molecular Diagnostics” MSc. Course ( Dept of Biology )
SUPERVISION OF POSTGRADUATE/GRADUATE STUDENTS
Supervision of 13 PhD theses (completed), 4 Master of Science theses (completed) and 2 Bachelor theses (completed)
Supervision of 5 PhD theses (on going)
RESEARCH INTERESTS
The role of steroid hormones and steroid receptors in the pathophysiology of various organs/systems in humans. Clinical studies concern a) the role of steroid receptor mutations and steroid receptor signalling, especially glucocorticoid and estrogen receptors, in the aetiopathology of diseases such as breast cancer, autoimmune diseases, osteoporosis, cardiovascular diseases, bipolar disorder, Alzheimer disease and b) development of methods for steroid determination in blood, such as gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry
(LC/MS/MS). Studies at the cellular level concern 1) the steroid receptor-mitochondria interaction and 2) screening of synthetic/natural compounds for their biological effects on cellular systems, such

2 as breast cancer cells, prostate cancer cells, osteoblasts, endometrial cells, endothelial cells by use of molecular biology techniques.
INTERNATIONAL COOPERATION
1. Joint Research Project with the University of Dundee (U.K.)-Department of Neurosciences, (Dr
K.C.Breen)
2. Karolinska Institute, Dept. of Biosciences and Nutrition, Sweden, (Prof. Karin Dahlman-Wright)
3. COST E39 Forests, Trees and Human Health and Wellbeing
4. Process Chemistry Centre, Wood and Paper Chemistry Turku /Ab, Abo Akademi University, Finland,
(Prof. Bjarne Holmbom)
NATIONAL COOPERATION
The research work has been carried out in cooperation with various Departments of the University of
Athens (these include Dept. of Pathophysiology, Dept. of Psychiatry, Dept. of Pediatrics, Dept. of
Cardiology, Dept. of Pathology, Dept. of Clinical Therapeutics, Dept. of Obstetrics and Gynecology,
Dept. for the Research of the Musculosceletal System, Dept. of Pharmacognosy and Medicinal
Chemistry, Dept. of Analytical Chemistry, Dept. of Pharmaceutical Chemistry), Research Institutes
(these include University Mental Health Research Institute, Institute of Biological Research and Biotechnology of the National Hellenic Research Foundation, Institute of Biology of NCSR Demokritos) and Hospitals (these include Eginition, Laiko, Attikon, Agia SophiaChildren’s Hospital, Aretaieion,
Evangelismos).
RESEARCH GRANTS
The research work has been funded by research grants from:

The Greek Ministry of Development- Greek General Secretariat of Research and Technology in cooperation with companies from Food Industry, Pharmaceutical Industry and Medical Diagnostic Centres (These include PAVE, PAVET, DESPRO, PRAXE, EPAN, Human Networks and International cooperation grants)

Ministry of Education (PENED and Herakleitos), and from the University of Athens Research
Account (Kapodistrias)
PUBLICATIONS (FULL ARTICLES) IN INTERNATIONAL JOURNALS
1. V. Moutsatsou and R.E. Oakey.
Oestriol and non protein bound oestriol concentrations in human peripheral plasma before labour and delivery .
J. Endocrinology (1986), 108:75 – 80.
2. V. Moutsatsou and R.E. Oakey.
Oestriol binding to plasma proteins.

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J. of Steroid Biochemistry (1988), 29 (3):319 – 323.
3. V. Moutsatsou, M.J. Levell, P. Sheridan and N. Bikas.
Failure of Carbenoxolone to Stimulate Plasma Cortisol.
Epitheorese Klinikes Farmakologias and Farmakokinetikes, International Edition, Edition 2: 57 –
60 (1988), Pharmakon Press.
4. P. Moutsatsou and C. E. Sekeris.
Estrogen and Progesterone receptors in the endometrium.
Annals of New York Academy of Sciences (1997) 816:99 – 115.
5. P. Moutsatsou, E. Kassi, G. Creatsas, S. Coulocheri, K. Scheller, C. E. Sekeris
Detection of oestrogen receptor variants in endometrium, myometrium, leiomyoma and peripheral blood mononuclear cells: comparison to variants present in breast cancer.
J. Cancer Res Clin Oncol (1998) 124 : 478 – 484.
6. P. Moutsatsou, L. Fountas, S. Coulocheri, E. Kassi, C. E. Sekeris.
The oestrogen receptor codon 10 polymorphism detected in breast cancer is also present in non – malignant cells.
J. Cancer Res Clin Oncol (1999) 125 : 214 – 218.
7. Fountas L., Moutsatsou P., Kastanias I., Tamouridis N., Tzanela M., Anapliotou M., Sekeris C. E.
The contribution of Vitamin D receptor Gene polymorphisms in Osteoporosis and Familial
Osteoporosis.
Osteoporosis Int (1999) 10(5) : 392 – 398.
8. Paraskevi Moutsatsou and Constantine E. Sekeris
The role of sex hormones on Carcinogenesis of the Endometrium.
EPITHEORESE KLINIKES FARMAKOLOGIAS KAI FARMAKOKINETIKES, INTERNATIONAL
EDITION (1999) 13 : 3
– 8.
9. P. Moutsatsou, A. Tsolakidou, G. Trikkas, C. Trougos, C. E. Sekeris
Glucocorticoid receptor alpha and beta isoforms are not mutated in bipolar affective disorder.
Molecular Psychiatry (2000) 5 : 196 - 202.
10. P. Moutsatsou, T. Kazazoglou, H. Fleischer
– Lambropoulos, A.M. G. Psarra, A. Tsiapara, C. E.
Sekeris, C. Stefanis, A. Vernadakis.
Expression of the Glucocorticoid receptor in early and late passage C-6 glioma cells and in normal astrocytes derived from aged mouse cerebral hemispheres.
International Journal of Developmental Neuroscience (2000), 18:329 - 335.
11. P. Moutsatsou, Kleoniki Liakou, Kieran Breen, Howard Cuckle.
The Serum expression of sialyltransferase in normal and Down Syndrome affected pregnancy.
Annals of Clinical Biochemistry (2000) 37:507 - 511.
12. Y. Gratsias, P. Moutsatsou, G. Chrysanthopoulou, S. Tsagarakis, N. Thalassinos, C.E. Sekeris.
Diurnal changes in glucocorticoid sensitivity in human peripheral blood samples.
Steroids (2000) 65:851-856.
13. Kassi EN, Vlachoyiannopoulos PG, Moutsopoulos HM, Sekeris CE and Moutsatsou P.
Molecular analysis of estrogen receptor alpha and beta in lupus patients.

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European Journal of Clinical Investigation (2001) 31:86-93.
14. Halabalaki M, Aligiannis N, Papoutsi Z, Mitakou S, Moutsatsou P, Sekeris C and Skaltsounis AL.
Three new Arylobenzofurans from Onobrychis ebenoides and evaluation of their binding affinity for the estrogen receptor.
Journal of Natural Products (2001) 63(12):1672-1674.
15. P. Moutsatsou, A.M.G. Psarra, A. Tsiapara, H. Paraskevakou, P. Davaris and C.E. Sekeris.
Localization of the glucocorticoid receptor in rat brain mitochondria.
Archives of Biochemistry and Biophysics (2001), 386(1):69-78.
16. A. Malamitsi-Puchner, J. Tziotis, D. Evangelopoulos, L. Fountas, G. Vlachos, G. Creatsas, C.E.
Sekeris, P. Moutsatsou.
Gene analysis of the N - terminal region of estrogen receptor - alpha in preeclampsia.
Steroids (2001), 66(9):695-700.
17. P. Moutsatsou.
Environmental estrogens and their effect on human's health
Epitheorese Klinikes Farmakologias and Farmakokinetikes International Edition (2001), 15:111-
122.
18. P. Moutsatsou
Human Networks. Environmental estrogens and their effect on human's health
Epitheorese Klinikes Farmakologias and Farmakokinetikes International Edition (2001), 15:109-
110.
19. V. Zoumpourlis, P. Papassava, A. Pintzas, P. Moutsatsou, K. Katsanakis
AP-1 transcription factors and steroid hormone receptors in multistage mouse skin carcinogenesis
Epitheorese Klinikes Farmakologias and Farmakokinetikes International Edition (2001), 15:123-
128.
20. K.M. Kasiotis, P. Magiatis, H. Pratsinis, A.-L. Skaltsounis, V. Abadji, A. Charalabous, P.
Moutsatsou, S.A. Haroutounian
Synthesis and biological evaluation of novel daunorubicin-estrogen conjugates
Steroids (2001), 66:785-791.
21. A.F. Tsolakidou, S.A. Coulocheris, C.E. Sekeris, P. Moutsatsou.
Screening the hormone binding domain of the human glucocorticoid receptor alpha for mutations by denaturing gradient gele electrophoresis
Journal of Biochemical and Biophysical Methods (2002), 51:233-241.
22. D. Vassiliadi, Y. Gratsias, S. Tsagarakis, E.Botoula, P. Trivizas, N. Thalassinos, P.Moutsatsou
Glucocorticoid sensitivity assessed in peripheral blood cells does not correlate with the feedback sensitivity of the hypothalamo-pituitary adrenal axis
Hormones (2002), 1(4):233-238.
23. D. Evangelopoulos, T. Makris, L. Fountas, S. Coulocheri, G. Stavroulakis, M. Kyriakidis, P.
Moutsatsou.
Estrogen receptor-alpha gene analysis in hypertensive premenopausal women
In: Molecular Mechanisms of Action of Steroid Hormone Receptors. Editors: M.Krstic-
Demonacos, C.Demonacos, Research Signpost (2003), 55-64.

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24. E. Kassi, P. Moutsatsou, C.E. Sekeris, H.M. Moutsopoulos, M.N. Manoussakis
Estrogen receptors in cultured epithelial cells from salivary glands of Sjögren's syndrome patients
Rheumatology (2003) 42(9):1120-1122.
25. D. Evangelopoulos, M. Alevizaki, J. Leakis, A. Cimponeriu, C. Papamichael, A. Kominakis, A. Kalofoutis, P. Moutsatsou.
Molecular analysis of the estrogen receptor alpha gene in man with coronary artery disease. Association with disease status
Clinica Chimica Acta (2003), 331:37-44.
26. A.F. Tsolakidou, S.A. Coulocheri, C.E. Sekeris, P. Moutsatsou.
Application of denaturing gradient gel electrophoresis (DGGE) to screen for mutations of the human glucocorticoid receptor α gene (hGRα)
Clinical Biochemistry (2003) 36:305-311.
27. S.A. Coulocheri, P. Moutsatsou.
A virtual enzyme-linked immunosorbent assay (ELISA) laboratory as a teaching tool for experimental work
Journal of Science Education (2003), July, No 2, Vol. 4 (in press)
28. K. Douroudis, K. Tarassi, G. Ioannidis, F. Giannakopoulos, P. Moutsatsou, N. Thalassinos, Chr.
Papasteriades.
Association of vitamin D receptor gene polymorphisms with bone mineral density in postmenopausal women of Hellenic origin
Maturitas (2003), 45:191-197.
29. M.M. Koufali, P. Moutsatsou, C.E. Sekeris, K.C. Breen
The dynamic localisation of the glucocorticoid receptor in rat C6 glioma cell mitochondria
Molecular Cellular Endocrinology (2003), 14:209(1-2):51-60.
30. P. Moutsatsou, C.E. Sekeris
Steroid receptors in the uterus
Ann New York Acad Sci (2003), 997:209-222.
31. L. Fountas, M. Anapliotou, C.E. Sekeris, P. Moutsatsou
Estrogen receptor alpha gene analysis in osteoporosis and familial osteoporosis
Osteoporosis International (2004), 15(12):948-956
32. A.F. Tsolakidou, S.A. Coulocheri, G. Trikas, C.E. Sekeris, P. Moutsatsou
Gene analysis of the ligand binding domain of the glucocorticoid receptor alpha in Alzheimer's disease
Clinica Chimica Acta (2004), 349(1-2):167-172
33. Z. Papoutsi, E.
Κassi, D. Papaevangliou, H. Pratsinis, V. Zoumpourlis, M. Halabalaki, S. Mitakou,
A. Kalofoutis, P. Moutsatsou
Plant 2-arylobenzofurans demonstrate a selective estrogen receptor modulator profile
Steroids (2004), 60(11-12):727-34
34.
E. Κassi, Z. Papoutsi, N. Fokialakis, S. Mitakou, P. Moutsatsou
Greek plant extracts exhibit selective estrogen receptor modulator (SERM)-like properties
Journal of Agricultural and Food Chemistry (2004), 52(23):6956-61

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35. E. Kassi, P.G. Vlachoyiannopoulos, A. Kominakis, H. Kiaris, H.M. Moutsopoulos, P. Moutsatsou
Estrogen receptor alpha gene polymorphism and systemic lupus erythematosus: a possible risk?
Lupus (2005), 14(5):391-8
36. Papoutsi Z, Kassi E, Tsiapara A, Fokialakis N, Chrousos GP, Moutsatsou P.
Evaluation of estrogenic/antiestrogenic activity of ellagic acid via the estrogen receptor subtypes Eralpha and Erbeta
J Agric Food Chem (2005), 53(20):7715-20
37. Dontas I, Halabalaki M, Moutsatsou P, Mitakou S, Papoutsi Z, Khaldi L, Galanos A, Lyritis GP.
Protective effect of plant extract from Onobrychis ebenoides on ovariectomy-induced bone loss in rats.
Maturitas (2006), 53(2):234-42.
38. Papoutsi Z, Kassi E, Mitakou S, Aligiannis N, Tsiapara A, Chrousos GP, Moutsatsou P.
Acteoside and martynoside exhibit estrogenic/antiestrogenic properties.
J Steroid Biochem Mol Biol. (2006), 98 (1):63-71
39. Spiliotaki M, Salpeas V, Malitas P, Alevizos V, Moutsatsou P.
Altered glucocorticoid receptor signaling cascade in lymphocytes of bipolar disorder patients.
Psychoneuroendocrinology. (2006), 31 (6):748-60
40. Grougnet R, Magiatis P, Mitaku S, Loizou S, Moutsatsou P, Terzis A, Cabalion P, Tillequin F,
Michel
Seco-Cycloartane triterpenes from Gardenia aubryi.
J Nat Prod. (2006), 69 (12):1711-4.
41. Lekatsas I, Koulouris S, Triantafyllou K, Chrisanthopoulou G, Moutsatsou-Ladikou P, Ioannidis G,
Thalassinos N, Kalofoutis A, Anthopoulos L
Prognostic significance of microalbuminuria in non-diabetic patients with acute myocardial infarction
Int J Cardiol. (2006), 106(2):218-23
42. Oikonomidou O, Vlachoyiannopoulos PG, Kominakis A, Kalofoutis A, Moutsopoulos HM,
Moutsatsou P.
Glucocorticoid receptor, nuclear factor kappaB, activator protein-1 and C-jun N-terminal kinase in systemic lupus erythematosus patients.
Neuroimmunomodulation. (2006), 13(4):194-204.
43. Papoutsi Z, Kassi E, Halabalaki M, Mitakou S, Moutsatsou P.
Evaluation of estrogenic/antiestrogenic activity of Onobrychis ebenoides extract - interaction with estrogen receptor subtypes ERalpha and ERbeta.
Toxicol In Vitro. (2007), 21(3):364-70.
44. Kassi E, Papoutsi Z, Pratsinis H, Aligiannis N, Manoussakis M, Moutsatsou P.
Ursolic acid, a naturally occurring triterpenoid, demonstrates anticancer activity on human prostate cancer cells.

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J Cancer Res Clin Oncol. (2007), 133(7):493-500.
45. Sgouros J, Galani E, Gonos E, Moutsatsou P, Belechri M, Skarlos D, Dionyssiou-Asteriou A.
Correlation of nm23-H1 gene expression with clinical outcome in patients with advanced breast cancer.
In Vivo. (2007), 21(3):519-22
46. Papoutsi Z, Kassi E, Fokialakis N, Mitakou S, Lambrinidis G, Mikros E, Moutsatsou P.
Deoxybenzoins are novel potent selective estrogen receptor modulators.
Steroids. (2007), 72(9-10):693-704.
47. Moutsatsou P.
The spectrum of phytoestrogens in nature: our knowledge is expanding.
Hormones (Athens) ( 2007), 6(3):173-93. Review.
48. Tziraki AA, Sotsiou FK, Tzirakis MA, Kominakis AP, Hadjiconstantinou VF, Nikolopoulou NI,
Moutsatsou PC.
Reduction in glucocorticoid receptors in renal biopsies of patients with lupus nephritis
Clin Biochem. (2007), 40(16-17):1188-93.
49. Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P.
Walnut extract (Juglans regia L.) and its component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity in the cell line KS483.
Br J Nutr. (2008), 99(4):715-22.
50. Moutsatsou P, Papavassiliou AG.
The glucocorticoid receptor signalling in breast cancer.
J Cell Mol Med. (2008), 12(1):145-63. Review.
51. Magnisali P, Dracopoulou M, Mataragas M, Dacou-Voutetakis A, Moutsatsou P.
Routine method for the simultaneous quantification of 17alpha-hydroxyprogesterone, testosterone, dehydroepiandrosterone, androstenedione, cortisol, and pregnenolone in human serum of neonates using gas chromatography-mass spectrometry.
J Chromatogr A. (2008), 1206(2):166-77.
52. Lekakis J, Ikonomidis I, Papoutsi Z, Moutsatsou P, Nikolaou M, Parissis J, Kremastinos DT.
Selective serotonin re-uptake inhibitors decrease the cytokine-induced endothelial adhesion molecule expression, the endothelial adhesiveness to monocytes and the circulating levels of vascular adhesion molecules.
Int J Cardiol. (2008), Nov 10.
53. Loizou S, Paraschos S, Mitakou S, Chrousos G, Lekakis I, Moutsatsou P.
Chios Mastic Gum Extract and Isolated Phytosterol Tirucallol Exhibit Anti-inflammatory
Activity in Human Aortic Endothelial Cells.
Exp Biol Med (Maywood). (2009), 234 (5):553-561.
54. Kassi E, Sourlingas TG, Spiliotaki M, Papoutsi Z, Pratsinis H, Aligiannis N, Moutsatsou P.
Ursolic Acid Triggers Apoptosis and Bcl-2 Downregulation in MCF-7 Breast Cancer Cells
Cancer Investigation (2009), 27 (7):723-733.
55. Tsiapara A, Jaakkola M, Chinou I, Graikou K, Tolonen T, Virtanen V, Moutsatsou P.
Bioactivity of Greek honey extracts on breast cancer (MCF-7), prostate cancer (PC-3) and endometrial cancer (Ishikawa) cells: Profile analysis of extracts

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Food Chemistry (2009), 116:702 –708.
56. Bei E, Salpeas V, Pappa D, Anagnostara C, Alevizos V, Moutsatsou P.
Phosphorylation status of glucocorticoid receptor, heat shock protein 70, cytochrome c and Bax in lymphocytes of euthymic, depressed and manic bipolar patients.
Psychoneuroendocrinology (2009), 34:1162-1175.
57. Strati A, Papoutsi Z, Lianidou E, Moutsatsou P.
Effect of ellagic acid on the expression of human telomerase reverse transcriptase (hTERT) alpha+beta+ transcript in estrogen receptor-positive MCF-7 breast cancer cells.
Clin Biochem. (2009), 42(13-14):1358-62.
58. Tsinti M, Kassi E, Korkolopoulou P, Kapsogeorgou E, Moutsatsou P, Patsouris E, Manoussakis
MN.
Functional estrogen receptors alpha and beta are expressed in normal human salivary gland epithelium and apparently mediate immunomodulatory effects.
Eur J Oral Sci. (2009), 117(5):498-505.
59. Loizou S, Lekakis I, Chrousos GP, Moutsatsou P. beta-Sitosterol exhibits anti-inflammatory activity in human aortic endothelial cells.
Mol Nutr Food Res. (2009), 2010 Apr;54(4):551-8.
60. Kassi E, Moutsatsou P.
Estrogen receptor signalling an d it’s relationship to cytokines in systematic lupus erythematosus.
J Biomed Biotechnol.(In press).
ARTICLES IN BOOKS
.
1. Moutsatsou P. Glucocorticoid receptor signaling and bipolar disorder: updates. In: Psychoneuroendocrinology Research Trends 2007; 361-380. Nova Science Publishers, Inc. ISBN 978-1-
60021-665-7.
2. Moutsatsou P.
Expert’s Commentary. Glucocorticoid receptor signaling and bipolar disorder: updates. In: Psychoneuroendocrinology Research Trends 2007; 381. Nova Science Publishers, Inc.
ISBN 978-1-60021-665-7.
3. Christos Gallis, Mariella Di Stefano, Paraskevi Moutsatsou, Tytti Sarjala, Vesa Virtanen, Bjarne
Holmbom, Joseph A. Buhagiar, and Al exandros Katalanos . “Forest Products with Health-
Promoting and Medicinal Properties “ in Book entitled “Forests, Trees and Human Health “ Nilsson,
K.; Sangster, M.; Gallis, C.; Hartig, T.; de Vries, S.; Seeland, K.; Schipperijn, J. (Eds.)1st Edition.,
2011, VIII, 452 p. 53 illus. in color., Hardcover ISBN: 978-90-481-9805-4

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4..E. Kassi and P. Moutsatsou. Glucocorticoid Receptor Signaling in Cancer and Inflamma tion" In
Book entitled "Glucocorticoids: Effects, Action Mechanisms, and Therapeutic uses" Nova Science
Publishers, Inc. 400 Oser Avenue, Suite 1600 Hauppauge, NY 11788 (in press)