COURSE OUTCOME
CHM 260
Basic Instrumental Analysis
Semester: July – Oct 2007
Text:
Principles of Instrumental Analysis, 5th edition, Skoog, Holler &
Nieman, Saunders, 1998
Goal:
Enable students to understand and apply scientific principles and
methods of chemical analysis.
Learning outcomes:
On completion of this course, students should be able to do the following:
1.
2
3.
4.
5.
6.
7.
8.
9.
10.
Identify and explain the different types of spectroscopic methods of analysis.
Identify and explain the different types of chromatographic methods of
analysis.
Identify and explain the different types of electromagnetic radiation.
Explain the principles and the working mechanism of Ultraviolet visible(UV)
Absorption Spectroscopy, Infrared Absorption (IR) Spectroscopy, Atomic
Absorption (AA) Spectroscopy, Flame Emission (FE) Spectroscopy, Gas
Chromatography (GC) and High Performance Liquid Chromatography
(HPLC).
Draw the schematic diagram of the related instruments in separation and
identification techniques and able to explain the function of each components
of the instrument. Differentiate between different instruments in terms of parts
and functions. (For example: radiation sources and sample cells, detectors)
Identify, distinguish and apply the appropriate chemical analysis methods in
determining unknowns.
Be able to use different instrumental methods of analysis to solve for specific
analytical problems. The student should be able to choose the appropriate
instrumental method for a particular investigation.
Interpret and evaluate analytical information from spectral data and diagnose
a physical problem and decide the most appropriate instrumental method for
further investigation and quantify the known compound graphically and
numerically.
Operate the UV, IR, AA, FE, GC, HPLC instruments with minimal supervision.
Acquire the science skills related to spectroscopic and chromatographic
analysis.
LESSON PLAN
Week
Total Hours
1
1
Contents
Diagnostics and Learning Skills • Conceptual Survey in
Chemical Concept Inventory• Learning Styles • Concept
Mapping
Introduction to Spectroscopic Methods of Analysis
1
1
Fundamental principles
Properties of electromagnetic radiation
Introduction to Spectroscopic Methods of Analysis
1
1
Molecular absorption spectroscopy.
Terms employed in absorption spectroscopy: Transmittance and
Absorbance
Beers’ Law: Application of Beer’s Law to mixtures, limitations to
the applicability of Beer’s law
2
Ultraviolet/Visible Spectrometry and Photometry
3
1
1
Molecular species that absorb UV/Vis radiation: Organic and
inorganic compounds
Instrumentation for UV spectroscopy
Ultraviolet/Visible Spectrometry and Photometry
4
1
1
Application of absorption measurement to qualitative analysis
Quantitative analysis by absorption measurement
Test 1
Infrared Absorption Spectroscopy
5
1
1
Molecular species that absorb IR radiation
Instrumentation and sample handling techniques
Infrared Absorption Spectroscopy
6
1
1
Qualitative applications: Structural Analysis
Qualitative applications: Structural Analysis
Atomic Spectroscopy based on Flame Atomization
7
1
1
Fundamental principle
Sample atomization, interferences
Atomic Spectroscopy based on Flame Atomization
8
1
1
Instrumentation: Hollow cathode lamp
Quantitative analysis
Flame Emission Spectroscopy
9
1
1
Fundamental principles
Comparison with atomic absorption
Test 2
Introduction to Chromatographic Separations
10
1
1
Migration rates of solutes, column efficiency and resolution
Qualitative/quantitative analysis
Gas Chromatography
11
1
1
Principles of GC
Instrumentation: Carrier gas, sample injection system, column
temperature (isothermal & thermal programming), types of
columns.
Gas Chromatography
12
2
Detectors: principles and application for flame ionization
detector, thermal conductivity detector, electron capture
detector
High Performance Liquid Chromatography
13
Principles of HPLC
Instrumentation: Mobile phase reservoirs and solvent system
(isocratic and gradient elution), pumping systems, detectors
1
1
High Performance Liquid Chromatography
14
Types of Liquid Chromatography (Adsorption, Partition – normal
and reverse phase, ion exchange and size exclusion); Principles
and applications
2
Test 3
15
Revision
16
FINAL EXAMINATION
Assessment:
Tests (3x)
Practical Reports
Final Examinations
:
:
:
30%
20%
50%
References:
a)
Spectrochemical Analysis, James D. Ingle, Jr. and Stanley R. Crouch
b)
Introduction to Spectroscopy, 3rd edition by Pavia, Lampman, Kriz
c)
Analytical Chemistry 6th edition , Gary D. Christian John Wiley &Sons, Inc.