AURA 2005 UND Project Titles & Mentors UND Dept. of Atmospheric Sciences Paul A. Kucera Box 9006, Grand Forks, ND 58202 777-6342 Fax: 777-5032 pkucera@aero.und.edu Project Title: Evaluation of In Situ and Remote Sensing Observations of the Atmosphere Research in the Northern Plains The Department of Atmospheric Sciences at the University of North Dakota has established an extensive atmospheric research site in the Northern Plain to compliment its recently upgraded (January 2004) C-Band polarimetric Doppler weather research radar, which is located on top of Clifford Hall. The atmospheric research site is located about 65 km south-southeast of Grand Forks. The research site resides on the Nature Conservancy Glacial Ridge Prairie Restoration Project, a 24,000 acre property that is currently being restored to natural prairie and wetlands from existing farmland. The extensive area of the property provides a unique opportunity to study atmospheric processes over a variety of temporal and spatial scales. The facility will include a dense network of rain gauges, several raindrop/snow size counters, a vertical wind profiler, snow sensors, microwave radiometer and a surface weather station to monitor environmental conditions. The research facility is designed to compliment existing hydrologic research activities at Glacial Ridge and will be a key to better understanding the relationship between the atmosphere and precipitation processes in the Northern Plains. The student will assist in developing a climatological observation database for detailed studies of how atmospheric conditions relate to the variability of precipitation by evaluating the field and radar observations. The student will gain experience testing, calibrating, deploying, and evaluating a variety meteorological instrumentation both in the laboratory and in the field. Also, the student will be exposed to weather radar operations along interpreting weather radar data. UND Dept. of Biology Dr. Brett J. Goodwin Box 9019, Grand Forks, ND 58202 777-2757 Brett.goodwin@und.nodak.edu Project Title: Insect Movement Behavior In Heterogeneous Prairie Landscapes Fax: 777-2623 Habitat loss and fragmentation has become ubiquitous and is considered a driving force behind current extinction rates. To understand how species are to survive in such fragmented landscapes it is necessary to understand how individuals move between isolated habitat patches. The ability to move between patches of habitat is a function of both the spatial structure of the landscape and the movement behavior of the organism moving. In this project, a suite of phytophagous insects associated with two native, patchily distributed prairie plants (goldenrod and stiff sunflower) will be used as a model system to address how movement behavior and landscape structure interact to determine interpatch movements. For each insect species studied we will: (1) measure movement behavior in response to landscape elements (e.g., patches of different vegetation and the edges between them); (2) assess the impact of weather conditions on movement behaviors; (3) use the observed movement behavior to parameterize an individual-based, spatially explicit computer simulation model to assess the impact of movement behavior and landscape structure on displacement and redistribution of individuals in landscapes; (4) field test the model predictions using mark-release-resight experiments. UND Dept. of Biology Dr. Peter Meberg Box 9019, Grand Forks, ND 58202 777-4674 Peter_meberg@und.nodak.edu Project Title: Regulation of synaptic function by the actin cytoskeleton Fax: 777-2623 Actin dynamics play an important role in the developmental outgrowth of neurites, the formation of synapses, and the modulation of synaptic activity at mature synapses. In addition, actin may play a significant role in neurodegenerative diseases, since actin depolymerization can protect against excitotoxicity, and epilepsy-induced sprouting of mossy fibers may be due to the reengagement of developmental growth mechanisms. The actin-depolymerizing factor (ADF) and cofilin family of proteins (AC) are important regulators of actin dynamics whose activity can be regulated by phosphorylation. AC is localized to synapses and growth cones in neurons, and AC over-expression increases actin turnover and neurite outgrowth. We recently found that AC activity is increased after kainate-induced seizures in regions of the hippocampus that undergo epileptogenic morphological changes, and have begun to investigate the signal pathways involved in the activity/phosphorylation regulation of AC proteins. Our next steps are to determine how changes in AC activity influence synaptic transmission. It will be determined if increased or decreased AC activity affects F-actin distribution and turnover, spine morphology, excitotoxicity, and calcium signaling. AC activity will be altered in a cell culture system by recombinant adenovirus-mediated expression of mutant forms of ACs and LIM kinase, the AC kinase, or by siRNA inhibition of AC protein expression. Additional work is planned to investigate synaptic activity-induced changes in the activity of other actin-binding proteins. UND Dept. of Chemical Engr. Dr. Michael D. Mann Box 7101, Grand Forks, DN 58202 777-3852 Fax: 777-3773 mikemann@mail.und.nodak.edu Project Title: Development of Techniques for Quantifying Sugar Yield when Making Ethanol from Corn Stover The UND Chemical Engineering Department is establishing a relationship with Purdue University for the development of new methods to harvest and process biomass. The harvesting methods, being developed at Purdue, include turning corn stocks/stover into a pumpable slurry that can subsequently be transported at relatively low costs by pipeline. It is expected that preprocessing of the corn stover will make it a premium feedstock for developing biorefineries. UND’s role in the development of this process is characterization of this feedstock to determine if it truly is a suitable feedstock for production of sugars and ethanol. The team would like to generate some preliminary data that can be used to help enhance the chance of developing successful proposals. As a part of this ARUA program, biomass samples will be obtained and methods developed to determine the yields and quality of sugars that can be produced from corn stover. Primarily, batch fermentation tests will be used to estimate yields, product composition, byproduct quality, and reactor sizes for commercial refineries. The AURA student will adapt methodology developed by the National Energy Renewable Laboratory to equipment available at UND. This project falls under the activities of SUNRISE (The SUtainable eNergy Research, Infrastructure and Education Initiative). UND Dept. of Chemical Engr. Dr. Darrin Muggli Box 7101, Grand Forks, ND 58202 777-2337 Fax: 777-3773 Darrin_muggli@und.nodak.edu Project Title: Using Novel Transient Reaction Techniques to Study Fuel-Cell Catalysts The goal of producing fuel-cell powered vehicles depends on discovering an effective means of delivering hydrogen to the fuel cell. Currently, the most promising hydrogen source would be one that will utilize the current gasoline delivery and storage infrastructure. Methanol is an ideal choice for this because of its high hydrogen-to-carbon ratio. The key to the success of this endeavor rests on designing an adequate catalyst that will produce effectively hydrogen from methanol. Rather than engaging in a trial-and-error approach to catalyst search, our method will involve first understanding how methanol forms hydrogen on current catalysts so that our development of improved catalysts will be guided by knowledge rather than by chance. The key to determining how methanol reacts on current hydrogen-producing catalysts is to analyze the surface processes on ‘real’ catalysts at reaction conditions. Currently, researchers use single-crystal studies to monitor surface reactions, but applicability is limited because ‘real’ catalysts are composed of many phases and crystal planes. Moreover, single-crystal studies are performed under ultra-high vacuum so that extrapolating data to typical industrial pressures is tenuous. On the other hand, studies of ‘real’ catalysts at reaction conditions are carried out at steady state by flowing reactants through a catalyst bed while monitoring the effluent. This produces little information about the fundamental surface processes because reactant adsorption, surface reaction, and product desorption occur simultaneously. This study proposes to utilize transient techniques, developed by the PI to study photocatalysis, to study the fundamental surface processes of hydrogen production from methanol. UND Dept. of Chemical Engr. Wayne Seames Box 7101, Grand Forks, ND 58202 777-2858 Wayneseames@mail.und.nodak.edu Project Title: Constructing a High Temperature Materials Test Chamber Fax: 777-3773 Novel combustion systems are the focus of a great deal of current research. One area of research is developing more efficient and effective ways of liberating the energy from existing conventional fuels such as coal and fuel oil. Other research is looking at liberating energy from novel fuel sources such as biomass, municipal sewage, and municipal wastes. Still further research is looking at space-based combustion environments. One factor common to all of these areas is the need to assess the performance of materials of construction for high temperature applications. The combustor is a high temperature, corrosive environment and most fuels generate solid deposits that exacerbate corrosion problems. The goal of this project is to build a high temperature materials test chamber to investigate material performance in combustion and gasification environments. This externally heated chamber will be connected to an existing simulated gas system to replicate high temperature combustion environments. Test coupons will be inserted into the chamber for use in material performance studies. Project challenges include the ability of the chamber to withstand frequent thermal cycling, the ability to make a gas-tight chamber that can also be easily accessed for cleaning and test coupon insertion/retraction, and the ability to control the temperature and gas composition environment within the chamber accurately. You must be mechanically inclined and willing to get dirty to complete this project. UND Dept of Chemistry Dr. Ewan Delbridge Box 9024, Grand Forks, ND 58202 777-2495 Fax: 777-2331 edelbridge@chem.und.edu Project Title: Synthesis and Characterization of Macrocyclic Ligands for Attachment to Lanthanide This project is concerned with synthesizing a series of different lanthanide catalyst precursors. All lanthanide catalysts synthesized will be used to polymerize lactide to polylactide. These studies are of broad interest because virtually all currently used polymers are derived from a finite resource – fossil fuels. To continue to meet the growing need, finding alternative polymers that are derived from renewable resources is a priority. One such polymer, polylactide (a polyester) is biodegradable and has several potential medical applications. Although polylactide has been shown to adopt many different geometrical structures (which greatly affect its properties), much has yet to be learned about how these different structures are obtained. Catalyst design is imperative to understanding the mechanism of polymerization which, in turn, affects the structure of the polymer. Lanthanide metal selection, along with ligand modification, will be used to systematically modify catalyst properties. Detailed studies evaluating catalyst performance and polymer microstructure will illuminate some of the mysteries of the polymerization mechanism. It is envisaged that an intimate understanding of the catalyst requirements will be gained from these studies. In addition, vital information gathered about polymer structure will be used as feedback to further improve, or fine-tune, catalyst precursor design. UND Dept. of Electrical Engr Dr. Hossein Salehfar,. Box 7165, Grand Forks, ND 58202 777-4432 Fax: 777-5253 hsalehfar@und.nodak.edu Dr. Michael Mann UND Dept. of Chemical Engr. Box 7101, Grand Forks, ND 58202 777-3852 Fax: 777-3773 Mikemann@mail.und.nodak.edu Project Title: Developing an Experiemental Data Base from a 500 kW Fuel Cell for use in Model Verfication Various researchers within the UND School of Engineering and Mines have been working to develop a viable program in hydrogen energy. One program supporting that effort is a project designed to address the issues required for the development of distributed wind-hydrogen energy systems. One of the current activities has focused around developing a computer model to simulate the operation of a PEM fuel cell. This model will be incorporated into a larger model that integrates wind, electrolysis, hydrogen storage, fuel cell, and a diesel generator to supply electrical power to a distributed load. The researchers would like to use their existing Ballard 500 kW fuel cell to gather data under a wide variety of simulated and experimental conditions to verify that the fuel cell model is accurate. The student will be responsible for gathering and interpreting data from the fuel cell. They will coordinate the experimentation with the modeling group to ensure that experiments are designed to obtain the proper data, and will work with the modelers in the comparison of the experimental and simulated data. This project falls under the activities of SUNRISE (The SUtainable eNergy Research, Infrastructure and Education Initiative). UND Dept of Mechanical Engr Dr. Matthew Cavalli Box 8359, Grand Forks, ND 58202 777-4389 Fax: 777-2271 Matthew.cavalli@mail.und.nodak.edu Dr. William Semke Box 8359, Grand Forks, ND 58202 777-4571 Fax: 777-2271 William.semke@mail.und.nodak.edu Project Title: Novel Materials for Improved Sensors Metal Centers – Potential Catalyst Precursors This project focuses on actively controlling the deformation and vibration behavior of polymer composite materials through the use of piezoelectric actuators. These actuators will be embedded within the composite structure and will either expand or contract when an electric voltage is applied. The deformation of these actuators depends on the properties of the applied electric signal and can be used to counteract undesirable deformations and vibrations in the material. Actively-controlled materials have application in various types of sensors where the relative position of each component must be known precisely or where the effects of external vibrations must be removed. Students working on this project can expect to spend time learning about the theories of composite material mechanics and piezoelectric materials, using these theories to predict the behavior of modified composite structures, and then verifying their calculations by fabricating and testing composite material samples. As part of the project, the students will also travel to local composite material manufacturers to gain an understanding for production and application of composite structures. UND Dept of Nursing Dr. Cindy M. Anderson Box 9025, Grand Forks, ND 58202 701-777-4354 Fax: 701-777-4096 cindyanderson@mail.und.nodak.edu Project Title: Fetal Growth Restriction: Consequences for Future Development of Hypertension Intrauterine fetal growth restriction has been linked to adverse health outcomes and the development of later disease, including hypertension. Little is know about the mechanisms contributing to hypertension in individuals with a history of fetal growth restriction. The objective of this project is to identify functional and biochemical changes for vascular signaling in resistance arteries of Sprague-Dawley rats born with fetal growth restriction. The central hypothesis is that resistance arteries of growth-restricted offspring demonstrate enhanced responsiveness due to an altered contractile signaling pathway, leading to hypertension. Methods for this study involve the surgical induction of reduced uteroplacental perfusion pressure in pregnant Sprague-Dawley rats, comprising the experimental group. The control group will undergo a sham procedure with perfusion to the utero-placental fetal unit unaffected. Offspring in both groups will be weighed within twelve hours of birth and then weekly until the conclusion of the study. Systolic blood pressure will be measured weekly beginning at four weeks of age using a tail-cuff apparatus. Animals will be euthanized at 6, 9 and 12 weeks of age. Mesenteric arteries will be isolated to determine expression of vascular signaling proteins (Western blot). Group differences will be determined by Student’s T-test. At the completion of this project, signaling mechanisms contributing to enhanced vascular responsiveness and hypertension will be identified. These data will assist in the identification of populations at risk for cardiovascular disease, to promote the basis of effective interventions to improve health outcomes for individuals with a history of growth restriction. UND Dept. of Nursing Dr. Donna L. Morris Box 9025 Grand Forks, ND 58202 DonnaMorris@mail.und.nodak.edu Project Title: Integrative Analysis of Menopause (I AM) 777-4529 Fax: 777-4096 Documentation of the relationship between lifestyle variables, such as physical activity and body composition, and menopause symptoms could offer important guidelines for women seeking safe symptom relief. The purpose of this descriptive study was to determine the relationship between a woman’s level of physical activity, body composition, Body Mass Index (BMI), and menopause symptoms. Other aspects of this study will examine dietary intake and hormonal profiles. In the pilot study, female employees from a Midwestern university, ages 42-65, were eligible to participate. Participants (N=62, mean age 50.2 years) completed questionnaires on menopause symptoms, medical history, dietary intake, and a physical activity interview. The following measurements were done: height, weight, three-site skinfold, and girth. BMI and age-adjusted body fat percentage were calculated. In the preliminary analysis, 29% of participants reported participation in moderate to vigorous exercise a minimum of three times weekly for at least 30 minutes each time. The remaining 71% reported sporadic, if any, exercise. Mean values were body fat 29.3%; BMI 29.6; waist 36.9 inches, and hips 43.9 inches. Most common menopause symptoms experienced were sleep disturbances, sweating, and physical and/or mental exhaustion. Regular exercisers reported fewer symptoms than less active participants. Controversy surrounds the effect of exercise and body composition on hormone levels and symptoms during menopause. Further analysis using step-wise multiple regression will provide additional clarification of the relationship of body composition and physical activity to menopause symptoms and may provide prescriptive exercise guidelines for reducing symptoms. UND Dept. of Pharmacology, Physiology, and Theraptutics Dr. Jonathan D. Geiger Box 9037, Grand Forks, ND 58202 777-2183 Fax: 777-4490 jgeiger@medicine.nodak.edu Project Title: Aging Effects on Levels/Actions of the Sleep Regulators Adenosine and Glycogen Sleep disorders, disturbances, and deprivation affect people of all ages, but are common among the aged. The consequences of such disturbances in sleep are important clinically because of increased morbidity, mortality and decreased quality of life, and economically because of reduced levels of productivity, decreased levels of learning and memory, and increased incidences of accidents. Treatments of sleep disorders are made difficult because there are gaps in our knowledge about the neurobiology of sleep – indeed sleep research has been referred to as a frontier of neuroscience because even the most basic of questions “Why do we sleep?” is unanswered. One substance increasingly implicated in sleep regulation is adenosine, an endogenous purine nucleoside. Multiple mechanisms and processes including its production, metabolism, release, and uptake (transport) regulate the levels of adenosine available to activate cell surface adenosine receptors classified as A1, A2A, A2B, and A3 on the basis of biochemical and pharmacological criteria and molecular structures, and changes in these mechanisms and processes with age, almost certainly, change the levels and actions of adenosine. Once activated, adenosine receptors have prfound effects on many different aspects of neural cells implicated in sleep regulation and sleep need including excitability and glycogen metabolism. However, at present very little is known about regulation of brain levels of adenosine in vivo and how this regulation is affected by age. Moreover, virtually nothing is known about the effects of adenosine on sleep in the aged and how increased wakefulness in the aged affects levels of adenosine and th expression of its actions. Since the early 1980’s, we have focused our work on determining the mechanisms by which and extent to which adenosine-enzymes and –transporters control the levels and actions of adenosine in vivo. The present application will take full advantage of our extensive background in the adenosine field to test specific hypotheses related to the control of brain adenosine levels as a function of increased age and wakefulness. Our overall hypotheses are that adenosine is an important regulator of sleep, that with increased age and wakefulness adenosine levels increase, and that increased levels of adenosine will result in increased levels of glycogen in specific sleep-related brain regions. UND Dept. of Pharmacology, Physiology, and, Therapeutics Dr. Van Doze Box 9037, Grand Forks, ND 58202 777-6222 vdoze@medicine.nodak.edu Dr. James Porter Box 9037 Grand Forks, ND 58202 porterj@medicine.nodak.edu UND Dept. of Biology Dr. Sally Pyle Box 9019, Grand Forks, ND 58202 777-3699 Sally.pyle@und.nodak.edu Project Title: Adrenergic Neuromodulation Multi-Investigator Group Fax: 777-4490 777-2296 Fax: 777-4490 Fax: 777-2623 The long-term goal of our research is to elucidate the biological effects of norepinephrine, an endogenous neurotransmitter in the brain. Norepinephrine has been implicated in neural functions as diverse as sleep, learning and memory, depression and epilepsy. The focus of this project is to examine the effects of norepinephrine on network activity in the hippocampal CA3 region, an area of the brain important in reinforcing memory processes, as well as prone to hyperexcitability. Our preliminary evidence suggests that norepinephrine has a biphasic (excitatory/inhibitory) effect on hippocampal CA3 network activity, which may be mediated by different (beta-1 vs. alpha-2) adrenergic receptors (ARs). These findings may be extremely important and could account for how norepinephrine both reinforces certain memory processes and attenuates hyperexcitable episodes in vivo. To test the hypothesis that the inhibitory actions of NE are mediated via alpha-2 ARs on pyramidal neurons, the following specific aims will be addressed: 1) Identify alpha-2 ARs expressed by hippocampal CA3 pyramidal neurons; 2) Define the role of alpha-2 AR activation in NE’s modulation of hippocampal pyramidal neuron excitability. Field potential and infrared-guided cell-attached patch-clamp electrophysiological recordings, pharmacological manipulation, and single cell RT-PCR methods will be used to achieve these aims. The results of this project will not only yield information about the underlying mechanisms by which NE modulates hippocampal neural networks, but will also identify novel receptor targets that potentially could be exploited pharmacologically. By extension, these studies may also provide insight into how norepinephrine enhances learning and memory, while inhibiting hyperexcitability. UND Dept. of Psychology F. Richard Ferraro Box 8380, Grand Forks, ND 58202 777-2414 F_ferraro@und.nodak.edu Project Title: Cross-Cultural Perspectives in Aging, Cognition & Neuropsychology Fax: 777-3454 For the past 20 years, I have been examining cognitive functions (i.e., memory, language, perception) in younger and older adults. Results have typically revealed that many cognitive mechanisms remain fairly stable with age. More recently, I have started to explore these aging and cognition mechanisms from a neuropsychological perspective. In particular, I have studied how the frontal lobe affects everyday behavior including planning, thinking, and impulsive decision-making. These studies have started to shine light on the complex interaction between behavior and brain function. One often neglected area of study in the aging, cognition, and neuropsychological area deal with older adults from different cultures. More specifically, Native American elderly adults have been a neglected group. In 2002 I edited a book (Minority and Cross-Cultural Aspects of Neuropsychological Assessment) that began to document in Native American elderly (and other cross-cultural groups) that lack of research in this area and offered up ways to begin more systematic and scientific studies into how elderly Native Americans perform on neuropsychological tests and how this performance relates to eventual dementia and Alzheimer’s disease. The present proposal will continue with that effort by analyzing data already collected as part of a grant examining dementia assessment in Native American elderly. Additionally, the current proposal will request support to collect similar data in non-Native elderly. The PI maintains lists of hundreds of non-Native elderly (age 55+) who can participate in the same project as the Native elderly, thereby allowing for Native and Non-Native groups comparisons. UND Dept. of Physics Dr. Kanishka Marasinghe Box 7129, Grand Forks, ND 58202 777-3560 Fax: 777-3523 k.marasinghe@und.edu Project Title: High Energy X-Ray Scattering Studies of the Atomic Structure of a Novel Laser Medium A family of novel vitreous rare earth-ultraphosphates are being investigated for potential use as a high energy laser (HEL) medium. In collaboration with scientists at the Argonne National Laboratory (ANL) and the University of MissouriRolla, we are working to produce vitreous rare earth-ultraphosphates whose atomic structure is “fine tuned” for maximum lasing efficiency. Probing the atomic structure of these materials using High-Energy X-ray Diffraction (HEXRD) is an important part of this project. HEXRD technique is relatively new and the data analysis process is quite complex. Hence, there is room and a need for further improvement in both the experimental technique and data analysis methodology. Over the past few months, researchers at UND and ANL have set out to develop a novel approach to data analysis that promises to increase considerably the accuracy of structural information extracted from HEXRD data. The AURA student will work on this exciting and challenging task. The student will first work at UND on existing HEXRD data and learn the physics behind the technique and data analysis methodology. He or she will then travel to one of the most modern synchrotron facilities in the world, the Advance Photon Source, at ANL to conduct a HEXRD experiment. Data from this experiment will be used to validate the novel data analysis procedure being developed by UND and ANL researchers. AURA student’s involvement in project should make him or her a strong candidate for one of the coveted DOE Science Undergraduate Internships in 2006. UND Dept. of Psychology Dr. Jeffrey Weatherly Box 8380 Grand Forks, ND 58202 777-3470 Jeffrey_weatherly@und.nodak.edu Dr. Adam Derenne Box 8380 Grand Forks, ND 58202 777-4215 Adam.derenne@und.nodak.edu Project Title: Investigating Behavioral Deficits in Mouse Models of Parkinson’s Disease Fax: 777-3454 Fax: 777-3454 Researchers at the School of Medicine at the University of North Dakota have developed several strains of mice that relate to the study of Parkinson’s Disease (PD). Specifically, there are strains naturally prone to developing PD, that are susceptible to developing PD when challenged, and that are resistant to developing PD even when challenged. We have joined in a collaborative effort with these researchers to determine whether these strains show behavioral differences in learning, memory, and motivation. If so, these differences could potentially lead to the identification of “behavioral markers” for the appearance of PD and ultimately to preventative measures and/or early detection of PD in humans. Students with an interest in pursuing a career in neuroscience, behavioral pharmacology, behavioral ecology, medical research, or psychology will benefit from this experience. UND Dept. of Psychology Dr. Jeffrey Weatherly Box 8380, Grand Forks, ND 58202 777-3470 Jeffrey_weatherly@und.nodak.edu Dr. Adam Derenne Box 8380, Grand Forks, ND 58202 777-4215 Adam.derenne@und.nodak.edu Project Title: Investigating Behavioral Deficits Produced by Pesticide Exposure Fax: 777-3454 Fax: 777-3454 The environment in the Northern Plains region, especially North Dakota, makes it unique to the study of neurodegenerative diseases produced by exposure to pesticides. Researchers at the University of North Dakota Energy & Environmental Research Center, as well as the School of Medicine, have begun the systematic study of such exposure as it relates to damage to certain neurological structures and pathways. We have joined in a collaborative effort with these researchers to determine how exposure to pesticides and the resulting neurological damage relates to behavioral changes in learning, memory, and motivation. Furthermore, we are especially interested in determining what level of exposure is necessary and/or sufficient to produce these behavioral differences. The outcome of this research will not only advance our understanding of how pesticide exposure alters behavior, but may also help to shed light on specific neurodengenerative diseases, such as Parkinson’s Disease, that are related to such exposure. Ideally, our research will also help identify “behavioral markers” for individuals who may have been exposed to pesticides, which will then serve to prevent further exposure. Students with an interest in pursuing a career in neuroscience, behavioral pharmacology, behavioral ecology, medical research, or psychology will benefit from this experience.