PROTEIN TECHNOLOGY CORE
SERVICE REQUISITION FORM
Name of the Customer:
Date:
Name of the Organization:
Type of Organization:
Academic
Non Academic
Email:
Phone:
Service/s Requested:
Expression system:
Cloning (Section A)
Bacterial- E. coli system
Expression (Section B)
Yeast- Pichia pastoris system
Purification (Section C)
Custom project
2D Gel electrophoresis
We came to know about the Protein Technology facility from
Declaration:
I/We declare that the information provided above is correct and formally ask C-CAMP to provide the
service requested as above. I/We agree to the terms and conditions (page 2) and understand that CCAMP Protein Core Technology is not obliged to provide the requested services based on this form.
I/We agree to be contacted for more details in the event that service is accepted or notified otherwise.
I/We agree to acknowledge the facility in any publications arising out this project.
Signature of Research Supervisor
Signature of Service Requestor
FOR OFFICE USE ONLY:
Expected deliverables for user at the end of service/s:
After Cloning____________
After Expression____________
After Purification____________
NB. Details provided to be included in experimental plan for user for approval
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
Terms and conditions:
Types of service workflows:
a)
Expression & purification services alone:
If client provides the construct containing insert for expression & purification services only, all information including the
sequence of insert/s, details on the construct/vector /s etc should be provided by the client. As it is mandatory to check
for purity and yield irrespective of the available data, facility will perform the following: DNA sequencing confirmation
of insert, expression, solubility, purity and yield check, before further large scale expression and purification.
b)
Purification services alone:
If the client requires purification only, all details requested and information such as, source, natural or recombinant and
in case of recombinant - nucleic acid and amino acid sequence of the insert, details of purification tag/s etc should be
provided by the client.
Deliverables and Payment terms:
a)
Results obtained at checkpoints shall be shared with the client. Deliverables (e.g. Constructs, strains etc) will be provided
to the client upon request and upon achievement of checkpoints. If not requested during the service, deliverables shall be
provided at the conclusion of the service.
b)
Multiple quotations and/or proforma invoices may be given to the client during the course of the service (dependent on
requirements of client and agreed payment schedule); however a single invoice will be raised for the complete service for
payment by the client.
Vector/strain (/s) used and provided by facility:
a)
For commercial/non-academic entities - Vectors/strains used by the facility for delivery of services and provided to the client
at the end of a service are to be used only for that given gene. Vectors/strains cannot be used to express other proteins
and are not to be shared freely with third party organizations. For commercialization of any technology based on the
vectors/strains provided, permission must be sought from the organization/company owning the rights/IP on the
vector/s or strain/s. If any infringement of this notice occurs, the facility shall not be held responsible for forthcoming
arbitrations.
b)
For academic/research institutions - Vectors and strains provided by the facility can be used ONLY for research purposes
and cannot be shared with third parties. It can only be used to express the protein that was cloned by the facility. If the
system is used for filing IP or for commercial activities, the institution and investigator are responsible for getting the
appropriate permission(s) from the company with the proprietary rights on the vectors/strains used.
Publications & Acknowledgements:
In the event that results from the service provided are to be published, depending on the intellectual input provided by CCAMP Protein facility, authorship can be discussed. In any event regardless of authorship, the Protein facility must be
acknowledged in any publication of results from the service.
Please note: The facility through its expertise and experience will assure the best of services as requested.
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In some cases, due to the unpredictability of proteins, variance and their functional behavior, it may not be possible to
obtain functionally active protein.
For activity studies, client may approach facility head for discussion on feasibility. This will be a separate custom service
request.
Appropriate internal positive controls will be used to assure the genomic integration of the gene in the P. pastoris genome
as well as for expression.
In case of service failures due to unforeseen reasons (e.g. no expression observed, unable to purify etc), client will still be
invoiced for the services rendered.
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
CLONING SERVICES
SECTION A:
Name of Gene:
Size (bp):
DNA to be synthesized by C-CAMP
Codon optimization required
Codon optimized sequence provided
DNA insert
provided by
customer
(construct)
Name of vector containing DNA
insert:
Vector of choice for cloning:
For Vectors available with the facility, please refer to table no. 1 & 2 in Annexure I
Concentration:
Minimum amount required 1-3 µg (150-200
ng/µl)
Restriction sites used for
cloning:
Check if gene insert in the
construct is optimized for
expression in the desired system
Codon optimization
required, if gene is not found
to be optimized
Please Note: Sequence optimized construct for one expression system is generally not recommended for other
systems
Source of DNA (organism):
Additional
information
regarding
cloning or previous supporting data –
Attached
Bio-safety level:
DNA sequence of gene of interest –
Attached
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
PROTEIN EXPRESSION SERVICES
SECTION B:
Name of Protein:
Size (kDa):
Construct provided
by customer
Name of vector containing DNA insert:
Fusion tag if any, Concentration:
Specify:
Minimum amount required 1-3 µg (150200 ng/µl)
Size of the DNA insert (bp):
Restriction
cloning:
Check if gene is optimized
sites
used
for
Codon optimization
required, if gene is not found
to be optimized
Please Note: Sequence optimized construct for one expression system is generally not recommended for other systems
Source (organism):
Bio-safety level:
Sequences attached:
Protein sequence
Expression optimization required
DNA sequence
Specific growth Conditions:
Please refer to (page 2 of Annexure I) for expression
optimization conditions recommended by the facility
Optimum temperature:
Strain/s:
Optimum pH:
Medium:
Optimum inducer concentration:
For Strains & media available with the facility,
please refer to table no. 1 & 2 in Annexure I
Expected protein yield after expression (if known):
Additional information regarding
expression conditions or previous
supporting data -Attached
Expression:
Intracellular
Storage & Stability details:
Solubility:
Extracellular
Periplasmic
Soluble
Insoluble
Solubility not known (Solubility check
needed)
Refolding required, if protein found to be
insoluble
Small scale expression trial- 100ml
Large scale expression- 2-20 liters only
Medium scale expression- 1 liter
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
PROTEIN PURIFICATION SERVICES
SECTION C:
Please Note: If only purification services are required, details about the protein to be filled in Section B
Sample provided (Purification service
only):
Crude
Nature of Protein:
Stable
Unstable
Partially purified
Affinity tag
Specify:
Anion-exchange
Specify:
Cation-exchange
Specify:
Gel filtration
Specify:
Others
Specify:
For purification columns available with the facility, please refer to table no. 3 in Annexure I
Purification standardization required
Protocol or publications for purification Attached
Additional information regarding
purification or previous supporting data if
any -Attached
Small scale purification trial to
check yield
pI value:
Buffer conditions:
Storage temperature:
Storage medium:
Lyophilized powder:
Solution:
mg
mg/ml, Quantity:
mg
Large scale purification
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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