Online Supporting Information for Maximizing return on conservation investment in the conterminous U.S. John C. Withey*, Joshua J. Lawler, Stephen Polasky, Andrew J. Plantinga, Erik J. Nelson, Peter Kareiva, Chad B. Wilsey, Carrie A. Schloss, Theresa M. Nogeire, Aaron Ruesch, Jorge Ramos Jr., and Walter Reid. *To whom correspondence should be addressed. e-mail: jwithey@fiu.edu This file includes: Figure S1 (pp. 2-3). Maps of individual county-level datasets used in the calculation of ROI: vertebrate species richness, estimated land cost ($/ha), percentage of county in protected areas, and the percentage of natural land cover in a county threatened with conversion. For each variable mapped, the legend is divided into bins of 10% quantiles, with units as shown in the legend. Table S1 (p. 4). Total number of species “in need of protection”, area selected, and estimated cost of land selected by three heuristics (full ROI, richness/cost, and richness alone, see Methods) across 20 protection targets. ‘Target’ refers to the minimum percentage of every species’ range that must be in protected area (sum of existing protected areas and lands selected for acquisition by the heuristic), before a species is taken off the list in subsequent steps of the heuristic. Results for the three heuristics, from left to right under each heuristic title, are 1) the total area selected for acquisition across all counties (km2), 2) the cost of those lands based on the county-specific estimate of land costs, in $US billion ($B), and 3) the percentage of lands acquired that are considered overprotection, i.e. lands that we assume would not be threatened with conversion (see Fig. 1). In the article Figure 3 shows maps associated with protection targets 1%, 7%, 14% and 20% for the heuristic using the full ROI. 1 Online Supporting Information for ‘Maximizing Return on Conservation Investment’ (Withey et al.) 2 Online Supporting Information for ‘Maximizing Return on Conservation Investment’ (Withey et al.) 3 Table S1. full ROI richness/cost richness alone Target 1% Species 27 km2 2,420 cost ($B) 0.3 overprotect 1.7% km2 2,300 cost ($B) 0.2 overprotect 10% km2 1,980 cost ($B) 0.3 overprotect 6.3% 2% 56 14,410 1.5 0.3% 13,200 1.3 11% 12,810 1.9 7.6% 3% 118 38,530 4.6 0.6% 39,390 4.5 13% 40,470 6.7 25% 4% 232 83,180 9.9 1.0% 81,460 9.8 13% 79,880 14.3 19% 5% 313 136,238 16.5 1.1% 128,960 15.4 14% 127,730 23.2 18% 6% 366 191,790 23.8 1.4% 181,230 21.9 17% 179,190 33.1 18% 7% 438 250,130 31.8 1.5% 237,260 28.7 19% 234,450 43.2 19% 8% 501 310,020 40.4 1.6% 299,480 36.1 22% 295,970 54.5 20% 9% 554 375,050 49.3 1.6% 365,650 43.7 24% 362,300 66.5 21% 10% 605 449,100 60.1 1.6% 436,540 51.7 26% 433,210 79.5 23% 11% 659 527,830 72.6 1.6% 509,400 59.9 29% 506,810 92.8 25% 12% 742 603,940 84.5 1.6% 585,860 68.6 31% 582,470 106.3 27% 13% 815 680,080 97.2 1.6% 664,500 77.6 33% 659,910 120.1 29% 14% 855 759,790 113.7 1.6% 744,520 86.9 36% 743,280 135.9 31% 15% 885 832,510 140.6 1.6% 824,390 96.1 37% 827,340 149.7 33% 16% 915 882,370 155.5 1.6% 906,430 105.7 39% 913,650 164.4 36% 17% 931 921,250 169.9 1.6% 989,280 115.3 41% 998,780 179.3 38% 18% 953 976,680 184.5 1.6% 1,072,810 125.1 43% 1,085,080 194.3 40% 19% 967 981,510 191.2 1.6% 1,157,490 135.2 45% 1,171,410 209.4 42% 20% 978 981,510 191.2 1.6% 1,243,190 145.1 47% 1,259,080 224.3 44% 4