Sarah Copeland Curriculum Vitae EDUCATION Master of Science in Forensic Science – DNA Evidence Expected September 2015 University of California, Davis Forensic Science Graduate Program Coursework Completed, Thesis Research in Progress: “Mitochondrial DNA Analysis of Highly Degraded Bone Samples Using Next Generation Sequencing” Relevant Coursework: Human Genetics & Genomics, Forensic DNA Analysis, Non-Human Forensic DNA, Molecular Techniques, Forensic Statistics, Quantitative Analysis, Scientific Evidence & Courtroom Testimony, Homicide Crime Scene Investigation Bachelor of Science in Biology – Physiology, Minor in Chemistry January 2008 San Francisco State University Relevant Coursework: General Chemistry, Organic Chemistry, Biochemistry, Immunology, Genetics, Cell Biology SKILLS Mitochondrial DNA (mtDNA) as well as nuclear DNA (nDNA) analysis Sanger Sequencing and capillary electrophoresis Next Generation Sequencing (NGS) including Roche 454 and Illumina MiSeq Library preparation for both amplicon and whole genome probe capture NGS DNA fragmentation using the Covaris M220 Focused Ultra Sonicator Linear array mtDNA oligonucleotide probe based assays DNA extraction from bone, buccal swabs, hair, blood, and tissue samples PCR and Real-Time quantitative PCR DNA quality analysis and quantification using fluorospectroscopy, gel electrophoresis, and Agilent 2100 Bioanalyzer PROFESSIONAL WORK EXPERIENCE Forensic Technician, Mitotyping Technologies, State College, PA March 2015 – Present Perform general lab duties such as restocking supplies; maintaining cleanliness standards in labs; mixing, aliquoting, and performing quality control testing of critical reagents; maintaining and calibrating equipment such as microscope, gel photo-documentation system, and thermal cyclers, among others. Receive, log in, label, and store evidence according to lab protocols. Then, return evidence and other sample material to outside agencies or customers after the conclusion of the case. Extract and amplify DNA (both nuDNA and mtDNA), perform STR analysis and/or Sanger sequence the questioned and known evidence samples according to established lab protocols. Attend internal and external training sessions and participate in required proficiency tests and validation studies. 1 Student Researcher, Children’s Hospital Oakland Research Institute July 2013 – March 2015 Student Researcher, UC Davis Forensic Science Center Performed independent research for my Master’s thesis project for the UC Davis Forensic Science program involving Next Generation Sequencing of mtDNA from degraded bone samples using the 454 GS Junior and Illumina MiSeq Next Generation Sequencing platforms Performed DNA extractions including bone and hair samples, PCR for mtDNA NGS sequencing, qPCR TaqMan assays for mtDNA/nDNA quantification and degradation analysis, library preparation for probe capture and PCR enrichment assays for NGS, gel electrophoresis and bioanalyzer for PCR and NGS library quality/quantity analysis, literature searches, and other duties as needed to assist personnel in Dr. Cassandra Calloway’s lab with their research on nuclear and mitochondrial DNA Assisted Dr. Calloway in teaching a 40 instruction hour lecture and laboratory course on mtDNA and HV+ HaploArray 5 and 10 plex linear array analysis in Croatia to Criminalists from Rjeka and members of the Forensic Science department at the University of Split on December 9-13, 2013. In January 2014, I assisted Dr. Calloway in teaching the Next Generation Sequencing portion of the lecture and laboratory course Mitochondrial DNA Analysis and Typing for the California Criminalistics Institute at the Jan Bashinski DNA Laboratory in Richmond, CA (~52 out of 84 total instruction hours). POSTERS, ABSTRACTS, & PRESENTATIONS Laura Wilson, BS; Sarah Copeland, BS; Gloria Dimick, MS; Charity Holland, MPH; Robert Bever, PhD; Jennifer McElhoe, PhD; Mitchell Holland, PhD. “Validation of mtDNA D-Loop Sequencing on the MiSeq.” International Society for Applied Biological Sciences Conference, Bol, Croatia. June 2015 (Poster) CONFERENCES & CONTINUING EDUCATION International Association of Blood Pattern Analysts; October 1-4, 2013 San Diego, CA o Workshop: Of What We Are Made (version 2): An Introduction to Basic Human Body Tissues; October 2, 2013. o Workshop: Forensic Pathology; October 3, 2013. American Academy of Forensic Sciences Annual Scientific Meeting; February 18-21, 2014 Seattle, WA Applied Biosystems 3130 Genetic Analyzer Training; March 26, 2015 State College, PA AFFILIATIONS Student Affiliate Member American Academy of Forensic Sciences 2 January 2014 - Present