Page 1
Curriculum Vitae
Name:
Born:
Reiji Kannagi
January 2, 1950, Tokyo
1974
1976-1980
1980-1982
Graduated Kyoto University, School of Medicine, Kyoto, Japan.
Doctor Course, Kyoto University, School of Medicine, Kyoto, Japan.
Research Fellow, Fred Hutchinson Cancer Research Center,
Program of Biochemical Oncology (Professor Hakomori), Seattle, Washington, USA.
1982-1985
Assistant Professor, Kyoto University, School of Medicine, Kyoto, Japan.
1982-1984
Visiting Assistant Professor, University of Washington, Seattle, Washington, USA.
1985-1991
Lecturer, Kyoto University, School of Medicine, Kyoto, Japan.
1991-2010
Head, Department of Experimental Pathology (Department of Molecular
Pathology as of April, 2000), Aichi Cancer Center, Research Institute, Nagoya,
Japan.
2009-Present Visiting Professor at Research Complex for Medicine Frontiers, Aichi Medical
University, and Visiting Scientist at Bioscience and Biotechnology Center, Nagoya
University, Nagoya, Japan.
2010-Present Visiting Professor at Graduate School of Pharmaceutical Sciences, Nagoya City
University, and Visiting Scientific Advisor, Department of Molecular Pathology,
Aichi Cancer Center, Research Institute, Nagoya, Japan.
Awards:
"Japanese Biochemical Society Young Scientist Award (1986)"
"Japanese Cancer Association Young Investigator Award (1987)"
"Kozakai Nozomu Award of Japanese Clinical Pathology Research Foundation (1994)"
"Princess Takamatsu Cancer Research Fund (1988, 1998)"
Academic Service:
J. Biol. Chem. (Editorial Board Member, American Society for Biochemistry and Molecular Biology)
Glycobiology (Associate Editor, American Society for Glycobiology)
Cancer Sci. (Associate Editor, Japanese Association for Cancer Research)
Glycoconj. J. (Editorial Board Member, International Glycoconjugate Organization)
Page 2
Bibliography
(Original papers)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
Kannagi, R. and Koizumi, K. Phospholipid-deacylating enzymes in rabbit platelets. Arch. Biochem. Biophys., 196:
534-542, 1979.
Kannagi, R. and Koizumi, K. Effect of different physical states of phospholipid substrates on partially purified
platelet phospholipase activity. Biochim. Biophys. Acta, 556: 423-433, 1979.
Kannagi, R., Koizumi, K., Hata-Tanoue, S., and Masuda, T. Mobilization of arachidonic acid from
phosphatidylethanolamine to phosphatidylcholine in rabbit platelets. Biochem. Biophys. Res. Commun., 96:
771-718, 1980.
Kannagi, R., Koizumi, K., and Masuda, T. Limited hydrolysis of platelet membrane phospholipids: On the proposed
phospholipase-susceptible domain in platelet membranes. J. Biol. Chem., 256: 1177-1184, 1981.
Kyoizumi, S., Kannagi, R., and Masuda, T. Membrane expression of Fc-receptors in cultured leukemic cell lines. I,
Induction of Fc-receptor in undifferentiated types of cells after passive modulation of lipid viscosity. J. Immunol.,
127: 2252-2256, 1981.
Hakomori, S., Nudelman, E., Kannagi, R., and Levery, S.B. The common structure in fucosyllactosamino-lipids
accumulating in human adenocarcinomas and its possible absence in normal tissue. Biochem. Biophys. Res.
Commun., 109: 36-44, 1982.
Kannagi, R., Kino, M., Saito, K., and Masuda, T. Phospholipid acyl chain metabolism during the differentiation of
murine leukemia cells; on the redistribution of poly-unsaturated acyl chains among phospholipids. Biochim.
Biophys. Acta, 712: 161-168, 1982.
Kannagi, R., Nudelman, E., Levery, S.B., and Hakomori, S. A series of human erythrocyte glycosphingolipids
reacting to the monoclonal antibody directed to developmentally-regulated antigen, SSEA-1. J. Biol. Chem., 257:
14865-14874, 1982.
Kannagi, R., Teshigawara, K., Noro, N., and Masuda, T. Transglutaminase activity during the differentiation of
macrophages. Biochem. Biophys. Res. Commun., 105: 164-171, 1982.
Kannagi, R., Nudelman, E., and Hakomori, S. Possible role of ceramide in defining the structure and function of
membrane glycolipids. Proc. Natl. Acad. Sci. USA, 79: 3470-3474, 1982.
Kannagi, R., Levine, P., Watanabe, K., and Hakomori, S. Recent studies of glycolipid and glycoprotein profiles and
characterization of the major glycolipid antigen in gastric cancer of a patient of blood group genotype pp(Tja -) first
studied in 1951. Cancer Res., 42: 5249-5254, 1982.
Kannagi, R., Fukuda, M.N., and Hakomori, S. A new glycolipid antigen isolated from human erythrocyte
membranes reacting with antibodies directed to globo-N-tetraosylceramide (globoside). J. Biol. Chem., 257:
4438-4442, 1982.
Nudelman, E., Hakomori, S., Kannagi, R., Levery, S.B., Yeh, M.Y., Hellström, K.E., and Hellström, I.
Characterization of a human melanoma-associated ganglioside antigen defined by a monoclonal antibody, 42. J.
Biol. Chem., 257: 12752-12756, 1982.
Kannagi, R., Papayannopoulou Th, , Nakamoto, B., Cochran, N.A., Yokochi, T., Stamatoyannopoulos, G., and
Hakomori, S. Carbohydrate antigen profiles of human erythroleukemia cell lines, HEL and K562. Blood, 62:
1230-1241, 1983.
Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., and Solter, D.
Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from
human teratocarcinoma cells. Eur. Mol. Biol. Orgn. J., 2: 2355-2361, 1983.
Kannagi, R., Levery, S.B., Ishigami, S., Hakomori, S., Shevinsky, L.H., Knowles, B.B., and Solter, D. New
globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a
developmentally-regulated antigen, SSEA-3. J. Biol. Chem., 258: 8934-8942, 1983.
Kannagi, R., Roelcke, D., Peterson, K.A., Okada, Y., Levery, S.B., and Hakomori, S. Characterization of an epitope
determinant structure in a developmentally-regulated glycolipid antigen defined by a cold agglutinin "Fl";
recognition of α-sialosyl and α-fucosyl groups in a branched structure. Carbohydr. Res., 120: 143-157, 1983.
Kannagi, R., Levery, S.B., and Hakomori, S. Sequential change of carbohydrate antigens associated with the
differentiation of murine Leukemia cells, M1: iI-antigenic conversion and shifting of glycolipid synthesis. Proc.
Natl. Acad. Sci. USA, 80: 2833-2848, 1983.
Kannagi, R., Stroup, R., Cochran, N.A., Urdal, D.L., Young, W.W.Jr., and Hakomori, S. Factors affecting
expression of glycolipid tumor antigens: Influence of ceramide composition and coexisting glycolipid on the
antigenicity of gangliotriaosylceramide in murine lymphoma cells. Cancer Res., 43: 4997-5005, 1983.
Nudelman, E., Kannagi, R., Hakomori, S., Parsons, M., Lipinski, M., Wiels, J., Fellous, M., and Tursz, T. A
glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody. Science, 220: 509-511,
1983.
Papayannopoulou Th, , Nakamoto, B., Yokochi, T., Chait, A., and Kannagi, R. Human erythroleukemia cell line
(HEL) undergoes a macrophage -like shift with TPA. Blood, 62: 832-845, 1983.
Page 3
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
Taki, T., Kawamoto, M., Seto, H., Noro, N., Masuda, T., Kannagi, R., and Matsumoto, M.
Differentiation-associated changes of glycolipid composition and metabolism in mouse myeloid leukemia cells
Induction of globotriaosylceramide and a galactosyltransferase. J. Biochem., 94: 633-644, 1983.
Bremer, E.G., Levery, S.B., Sonnio, S., Ghidoni, R., Canevari, S., Kannagi, R., and Hakomori, S. Characterization
of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neo-plastic
epithelial cells of human mammary gland. J. Biol. Chem., 259: 14773-14777, 1984.
Clausen, H., Watanabe, K., Kannagi, R., Levery, S.B., Nudelman, E., Arao-Tomono, Y., and Hakomori, S. Blood
Group A glycolipid (Ax) with globo-series structure which is specific for blood group A1 erythrocytes: One of the
chemical bases for A1 and A2 distinction. Biochem. Biophys. Res. Commun., 124: 523-529, 1984.
Hakomori, S., Nudelman, E., Levery, S.B., and Kannagi, R. Novel fucolipids accumulating in human
adenocarcinoma: I. Glycolipids with di- or trifucosylated type 2 chain. J. Biol. Chem., 259: 4681-4685, 1984.
Hatanaka, M., Yoshimura, N., Murakami, T., Kannagi, R., and Murachi, T. Evidence for membrane-associated
calpain I in human erythrocytes; detection by an immunoelectrophoretic blotting method using monospecific
antibody. Biochemistry, 23: 3272-3276, 1984.
Kannagi, R., Levery, S.B., and Hakomori, S. Hybrid type glycolipids (Lacto-ganglio series) with a novel branched
structure: Their presence in undifferentiated murine leukemia cells and their dependence on differentiation. J. Biol.
Chem., 259: 8444-8451, 1984.
Kannagi, R., Levery, S.B., and Hakomori, S. Blood group H antigen with globo-series structure: Isolation and
characterization from human blood group O erythrocytes. FEBS Lett., 175: 397-401, 1984.
Okada, Y., Kannagi, R., Levery, S.B., and Hakomori, S. Glycolipid antigens with blood group I and i specificities
from human adult and cord erythrocytes. J. Immunol., 133: 835-842, 1984.
Takano, E., Kitahara, A., Kannagi, R., and Murachi, T. Enzyme immunoassay of calpain I and calpastatin and its
application to the analysis on human erythrocyte hemolysate. J. Appl. Biochem., 6: 117-125, 1984.
Takano, E., Yumoto, N., Kannagi, R., and Murachi, T. Molecular diversity of calpastatin in mammalian organs.
Biochem. Biophys. Res. Commun., 122: 912-917, 1984.
Yoshimura, N., Ohtsuki, H., Hamakubo, T., Kitahara, A., Kannagi, R., and Murachi, T. Distribution of calpain in
various organs of rat: An immunohistochemical study. Biomed. Res., 5: 419-424, 1984.
Fukushi, Y., Kannagi, R., Hakomori, S., Shepard, T., Kulander, B.G., and Singer, J.W. Localization and distribution
of difucoganglioside (VI3NeuAcIII3V3Fuc2nLc6) in normal and tumor tissues defined by its monoclonal antibody
FH6. Cancer Res., 45: 3711-3717, 1985.
Kannagi, R., Levery, S.B., and Hakomori, S. Lea-active heptaosylceramide, a hybrid of type 1 and 2 chain, and the
pattern of glycolipid with Lea, Leb, X (Lex), and Y (Ley) determinants in human blood cell membranes (Ghosts):
Evidence that type 2 chain can elongate repetitively but type 1 cannot. J. Biol. Chem., 260: 6410-6415, 1985.
Kannagi, R., Yamagata, Y., and Murachi, T. Expected properties of phospholipase-susceptible domain in platelet
membranes. Adv. in Prostaglandin. Thromboxane. Leukotriens. Res., 15: 125-127, 1985.
Kitahara, A., Ohtsuki, H., Kirihata, Y., Yamagata, Y., Takano, E., Kannagi, R., and Murachi, T. Selective
localization of calpain I (the low Ca2+-requiring form of Ca2+-dependent cysteine proteinase) in B-cells of human
pancreatic islets. FEBS Lett., 184: 120-124, 1985.
Nichols, E.J., Kannagi, R., Hakomori, S., Krants, M.J., and Fuks, A. Carbohydrate determinants associated with
carcinoembryonic antigen (CEA). J. Immunol., 135: 1911-1913, 1985.
Sakihama, T., Kakidani, H., Zenita, K., Yumoto, N., Kikuchi, T., Sasaki, T., Kannagi, R., Nakanishi, S., Ohmori, M.,
Takio, K., Titani, K., and Murachi, T. A new putative Ca2+-binding protein: Structure of the light subunit of porcine
calpain elucidated by molecular cloning and protein sequence analysis. Proc. Natl. Acad. Sci. USA, 82: 6075-6079,
1985.
Teshigawara, K., Kannagi, R., Noro, N., and Masuda, T. Possible involvement of transglutaminase in endocytosis
and antigen presentation. Microbiol. Immunol., 29: 737-750, 1985.
Clausen, H., Levery, S.B., Kannagi, R., and Hakomori, S. Novel blood group H antigen exclusively expressed in
blood group A and AB erythrocytes (type 3 Chain H) I, isolation and chemical characterization. J. Biol. Chem.,
261: 1380-1387, 1986.
Hamakubo, T., Matus, A., Kannagi, R., and Murachi, T. Distribution of calpains I and II in rat brain. J.
Neuroscience, 6: 3103-3111, 1986.
Hamakubo, T., Ueda, M., Fucharoen, S., Wilairat, P., Pimainork, Y., Kannagi, R., and Murachi, T. Estimation of
calpain and calpastatin levels in thalassemic red blood cells and the study of membrane protein. ICSU. Short.
Reports., 6: 624-625, 1986.
Kannagi, R., Fukushi, Y., Tachikawa, T., Noda, A., Shin, S., Shigeta, K., Hiraiwa, N., Fukuda, Y., Inamoto, T.,
Hakomori, S., and Imura, H. Quantitative and qualitative characterization of cancer-associated serum glycoprotein
antigens expressing fucosyl or sialosyl-fucosyl type 2 chain polylactosamine. Cancer Res., 46: 2619-2626, 1986.
Kitahara, A., Takano, E., Ohtsuki, H., Kirihata, Y., Yamagata, Y., Kannagi, R., and Murachi, T. Reversed
distribution of calpains and calpastatin in human pituitary gland and selective localization of the calpastatin in
ACTH-producing cells as demonstrated by an immunohistochemical study. J. Clin. Endocrinol. Metab., 63:
343-348, 1986.
Page 4
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
Miyachi, Y., Yoshimura, N., Suzuki, S., Hamakubo, T., Kannagi, R., Imamura, S., and Murachi, T. Biochemical
properties and immunohistochemical localization of calpain in human skin. J. Invest. Dermatol., 86: 346-349,
1986.
Okada, Y., Arima, T., Nagashima, H., Fukushi, Y., Kannagi, R., and Kaizu, T. Human hepatocellular
carcinoma-associated changes of glycosphingolipids detected by two monoclonal antibodies FH2 and 1B9. J.
Hepatol., 2: 430-440, 1986.
Takano, E., Maki, M., Hatanaka, M., Mori, H., Zenita, K., Sakihama, T., Kannagi, R., Marti, T., Titani, K., and
Murachi, T. Evidence for the repetitive domain structure of pig calpastatin as demonstrated by cloning of
complementary DNA. FEBS Lett., 208: 199-202, 1986.
Takano, E., Kitahara, A., Sasaki, T., Kannagi, R., and Murachi, T. Two different molecular species of porcine
calpastatin: Structural and functional relationship between 107-KDa and 68-KDa molecules. Biochem. J., 235:
97-102, 1986.
Andou, Y., Imamura, S., Yamagata, Y., Kikuchi, T., Murachi, T., and Kannagi, R. High-performance liquid
chromatographic assay of transglutaminase and its application to the purification of human erythrocyte
transglutaminase and platelet factor XIII. J. Biochem., 101: 1331-1337, 1987.
Andou, Y., Imamura, S., Yamagata, Y., Kitahara, A., Saji, H., Murachi, T., and Kannagi, R. Platelet factor XIII is
activated by calpain. Biochem. Biophys. Res. Commun., 144: 484-490, 1987.
Fujimoto, J., Hata, J., Ishii, E., Tanaka, S., Kannagi, R., Ueyama, Y., and Tamaoki, N. Differentation antigens
defined by mouse monoclonal antibodies against human germ cell tumors. Lab. Invest., 57: 350-358, 1987.
Kitahara, A., Ohtsuki, H., Yamagata, Y., Murachi, T., and Kannagi, R. Selective localization of tissue type
transglutaminase in adrenocorticotropin-producing cells of human pituitary. J. Clin. Endocrinol. Metab., 65:
885-890, 1987.
Maki, T., Takano, E., Mori, H., Kannagi, R., Murachi, T., and Hatanaka, M. Repetitive region of calpastatin is a
functional unit of the proteinase inhibitor. Biochem. Biophys. Res. Commun., 143: 300-308, 1987.
Shigeta, K., Kirihata, Y., Ito, Y., Ogawa, T., Hakomori, S., and Kannagi, R. Monoclonal antibody directed to a
chemically-synthesized cancer-associated glycosphingolipid antigens. J. Biol. Chem., 262: 1358-1362, 1987.
Ando, Y., Miyachi, Y., Imamura, S., Kannagi, R., and Murachi, T. Purification and characterization of calpain from
pig epidermis and its action on keratin. J. Invest. Dermatol., 90: 26-30, 1988.
Ando, Y., Imamura, S., Murachi, T., and Kannagi, R. Calpain activates two transglutaminases from porcine skin.
Arch. Dermatol. Res., 280: 380-384, 1988.
Hiraiwa, N., Iida, N., Ishizuka, I., Itai, S., Shigeta, K., Fukuda, Y., Kannagi, R., and Imura, H. Monoclonal
antibodies directed to a disulfated glycosphingolipid associated with human hepatocellular carcinoma, SB1a
(GgOse4Cer- II3IV3-bis-sulfate). Cancer Res., 48: 6769-6774, 1988.
Itai, S., Arii, S., Tobe, R., Kitahara, A., Kim, Y-C., Yamabe, H., Ohtsuki, H., Kirihata, Y., Shigeta, K., and Kannagi,
R. Significance of 2-3 and 2-6 sialylation of Lewis A antigen in pancreas cancer. Cancer, 61: 775-787, 1988.
Kannagi, R., Kitahara, A., Itai, S., Zenita, K., Shigeta, K., Tachikawa, T., Noda, A., Hirano, H., Abe, M., Shin, S.,
Fukushi, Y., Hakomori, S., and Imura, H. Quantitative and qualitative characterization of human cancer-associated
serum glycoprotein antigens expressing epitopes consisting of sialyl or sialyl-fucosyl type 1 chains. Cancer Res.,
48: 3856-3863, 1988.
Levery, S.B., Nudelman, E., Kannagi, R., Symington, F.W., Andersen, N.H., Clausen, H., Baldwin, M., and
Hakomori, S. 1H-NMR-Analysis of type-2 chain lacto-gangliosides. Confirmation of structure of a novel
cancer-associated fucoganglioside,α-NeuAc-(2→6)-α-D-Galp-(1→4)-β-D-GlcpNAc-(1→3)-β-D-Galp-(1→
4)-[ α -L-Fucp-(1 → 3)]- β -D-GalpNAc-(1 → 3)- β -D-Galp-(1 → 4)- β - D-Glcp-(1 → 1)-Cer
(VI6NeuAcIII3FucnLc6Cer). Carbohydr. Res., 178: 121-144, 1988.
Miyake, M., Ito, M., Hitomi, S., Ikeda, S., Taki, T., Kurata, M., Hino, A., Miyake, N., and Kannagi, R. Generation
of two monoclonal antibodies that can discriminate N-glycolyl and N- acetyl neuraminic acid residues of GM2
gangliosides and distribution of the antigens in human lung cancer. Cancer Res., 48: 6154-6160, 1988.
Miyake, M., Zenita, K., Tanaka, O., Okada, Y., and Kannagi, R. Stage-specific expression of SSEA-1 related
antigens in the developing lung and its relation to the distribution of these antigens in lung cancers. Cancer Res.,
48: 7150-7158, 1988.
Ohno, T., Inaba-Miyama, M., Masuda, T., Kannagi, R., Kanoh, T., and Uchino, H. Biochemical analysis of
inhibitory effects of a lymphokine suppressive B-cell factor on the activation process of resting B-cells. Cell
Immunol., 112: 27-39, 1988.
Takano, E., Park, Y.H., Kitahara, A., Yamagata, Y., Kannagi, R., and Murachi, T. Distribution of calpains and
calpastatin in human blood cells. Biochemistry Int., 16: 391-395, 1988.
Takano, E., Maki, M., Mori, H., Hatanaka, M., Marti, T., Titani, K., Kannagi, R., and Murachi, T. Identification of
repetitive domain structures within pig heart calpastatin. Biochemistry, 27: 1964-1972, 1988.
Yamagata, Y., Shigeta, K., Shin, S., Tachikawa, T., Noda, A., Ohe, Y., Masuda, T., Imura, H., Murachi, T., and
Kannagi, R. Glycolipid-directed FH-6 monoclonal antibody recognizes high-molecular-weight glyco-protein
antigen carrying sialyl Lex-i determinant in the culture supernatant of PC-9 cells. J. Mol. Recog., 1: 111-116, 1988.
Zenita, K., Kirihata, Y., Kitahara, A., Shigeta, K., Higuchi, K., Hirashima, K., Murachi, T., Miyake, M., Takeda, T.,
Page 5
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
and Kannagi, R. Fucosylated type 2 chain polylactosamine antigens in human lung cancer. Int. J. Cancer, 41:
344-349, 1988.
Ando, Y., Imamura, S., Hong, Y.M., Owada, M.K., Kakunaga, T., and Kannagi, R. An enhancement of calcium
sensitivity of lipocortin I in phospholipid binding induced by the limited proteolysis at N-terminus as analyzed by
phospholipid-CPG affinity column chromatography. J. Biol. Chem., 264: 6948-6955, 1989.
Ando, Y., Imamura, S., Owada, M.K., Kakunaga, T., and Kannagi, R. Cross-linking of lipocortin I and enhancement
of its calcium sensitivity by transglutaminase. Biochem. Biophys. Res. Commun., 163: 944-951, 1989.
Mori, Y., Fukuma, K., Adachi, Y., Shigeta, K., Kannagi, R., Tanaka, H., Sakai, M., Kuribayashi, K., Uchino, H., and
Masuda, T. Characterization of parietal cell autoantigens involved in neonatal thymectomy-induced murine
autoimmune gastritis using monoclonal autoantibodies. Gastroenterology, 97: 364-375, 1989.
Ogata, N., Yonekawa, Y., Taki, W., Kannagi, R., Murachi, T., and Kikuchi, H. Degradation of neurofilament
proteins in cerebral ischemia. J. Neurosurg., 70: 103-107, 1989.
Ohmori, K., Yoneda, T., Shigeta, K., Hirashima, K., Kanai, M., Itai, S., Sasaoki, T., Arii, S., Arita, H., and Kannagi,
R. Sialyl SSEA-1 antigen as a carbohydrate marker of human natural killer cells and immature lymphoid cells.
Blood, 74: 255-261, 1989.
Takano, E., Hamakubo, T., Kawatani, Y., Ueda, M., Kannagi, R., and Murachi, T. Multiple forms of calpastatin in
pig brain. Biochemistry Int., 19: 633-643, 1989.
Ando, Y., Imamura, S., and Kannagi, R. Human epidermis contains coagulation factor XIII. Arch. Dermatol. Res.,
282: 656-667, 1990.
Hiraiwa, N., Tsuyuoka, K., Li, Y.T., Tanaka, M., Seno, T., Okubo, Y., Fukuda, Y., Imura, H., and Kannagi, R.
Gangliosides and sialoglycoproteins carrying a rare blood group antigen determinant, Cad, associated with human
cancers as detected by specific monoclonal antibodies. Cancer Res., 50: 5497-5503, 1990.
Hiraiwa, N., Fukuda, Y., Imura, H., Tadano-Aritomi, K., Nagai, K., Ishizuka, I., and Kannagi, R. Accumulation of
highly acidic sulfated glycosphingolipids in human hepatocellular carcinoma defined by a series of monoclonal
antibodies. Cancer Res., 50: 2917-2928, 1990.
Hirashima, K., Zenita, K., Kitahara, A., Ishihara, G., Harada, R., Ohmori, K., Hirohashi, S., Akiyama, S., and
Kannagi, R. High idiotypic connectivity of the VH7183-encoded antibodies directed to a murine embryonic
carbohydrate antigen, Lewis Y, as ascertained by syngenic anti-idiotype monoclonal antibodies. J. Immunol., 145:
224-232, 1990.
Itai, S., Nishikata, J., Takahashi, N., Arii, S., Tobe, T., Tanaka, O., and Kannagi, R. Differentiation-dependent
expression of I- and sialyl I-antigens in the developing lung of human embryos and in lung cancers. Cancer Res.,
50: 7603-7611, 1990.
Miyake, M., Hashimoto, K., Ito, M., Hitomi, S., and Kannagi, R. The abnormal occurrence and the
differentiation-dependent distribution of N-acetyl and N-glycolyl species of the ganglioside GM2 in human germ cell
tumors: A study with specific monoclonal antibodies. Cancer, 65: 499-505, 1990.
Naito, M., Imamura, M., Kannagi, R., and Tobe, T. Production of monoclonal antibody to human esophageal cancer
cell line. Jap. J. Surg., 20: 170-179, 1990.
Zenita, K., Hirashima, K., Shigeta, K., Hiraiwa, N., Takada, A., Fujimoto, E., Hashimoto, K., Hakomori, S., and
Kannagi, R. Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to
cancer-associated ganglioside antigens having various sialic acid linkages. J. Immunol., 144: 4442-4451, 1990.
Ando, Y., Imamura, S., Owada, M.K., and Kannagi, R. Calcium-induced intracellular cross-linking of lipocortin I
by tissue transglutaminase in A431 cells: Augmentation by membrane phospholipids. J. Biol. Chem., 266:
1101-1108, 1991.
Itai, S., Nishikata, J., Yoneda, T., Ohmori, K., Tsunekawa, S., Hiraiwa, N., Yamabe, H., Arii, S., Tobe, T., and
Kannagi, R. Tissue distribution of sialyl 2-3 and 2-6 Lewis a antigens and the significance of serum 2-3/2-6 sialyl
Lewis a antigen ratio for the differential diagnosis of malignant and benign disorders of the digestive tract. Cancer,
67: 1576-1587, 1991.
Mori, Y., Hosono, M., Murakami, K., Katoh, H., Yoshikawa, Y., Kuribayashi, K., Kannagi, R., Sakai, M., Okuma,
M., and Masuda, T. Genetic studies on experimental autoimmune gastritis induced by neonatal thymectomy using
recombinant inbred strains between a high-incidence strain, BALB/c, and a low-incidence strain, DBA/2. Clin. Exp.
Immunol., 84: 145-152, 1991.
Takada, A., Ohmori, K., Takahashi, N., Tsuyuoka, K., Yago, K., Zenita, K., Hasegawa, A., and Kannagi, R.
Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A.
Biochem. Biophys. Res. Commun., 179: 713-719, 1991.
Miyake, M., Taki, T., Kannagi, R., and Hitomi, S. First establishment of a human monoclonal antibody directed to
sulfated glycosphingolipids SM4s-Gal and SM4g, from a patient with lung cancer. Cancer Res., 52: 2292-2297, 1992.
Tuo, X.H., Itai, S., Nishikata, J., Mori, T., Tanaka, O., and Kannagi, R. Stage-specific expression of
cancer-associated type 1 and type 2 chain polylactosamine antigens in the developing pancreas of human embryos.
Cancer Res., 52: 5744-5751, 1992.
Uemura, K., Inagaki, H., Wada, Y., Nakanishi, K., Asai, K., Kato, T., Ando, Y., and Kannagi, R. Identification of
immuno-reactive lipocortin 1-like molecules in serum and plasma by an enzyme immunoassay for lipocortin 1.
Page 6
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
Biochim. Biophys. Acta, 1119: 250-255, 1992.
Ikai, K., Shimizu, K., Ando, Y., Furukawa, F., Imamura, S., and Kannagi, R. Immunohistochemical localization of
lipocortins in normal and psoriatic human skin. Arch. Dermatol. Res., 285: 296-299, 1993.
Kaneko, T., Iba, Y., Zenita, K., Shigeta, K., Nakano, K., Itoh, W., Kurosawa, Y., Kannagi, R., and Yasukawa, K.
Preparation of mouse-human chimeric antibody to fucosylated stage-specific embryonic antigen-1. J. Biochem.,
113: 114-117, 1993.
Ohmori, K., Takada, A., Ohwaki, I., Takahashi, N., Furukawa, Y., Maeda, M., Kiso, M., Hasegawa, A., Kannagi, M.,
and Kannagi, R. A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively
expressed on helper memory T cells. Blood, 82(9): 2797-2805, 1993.
Ohmori, K., Takada, A., Yoneda, T., Buma, Y., Hirashima, K., Tsuyuoka, K., Hasegawa, A., and Kannagi, R.
Differentiation-dependent expression of sialyl stage-specific embryonic antigen-1 and I-antigens on human
lymphoid cells and its implications for carbohydrate-mediated adhesion to vascular endothelium. Blood, 81:
101-111, 1993.
Sawada, M., Takada, A., Ohwaki, I., Takahashi, N., Tateno, H., Sakamoto, J., and Kannagi, R. Specific expression
of a complex sialyl Lewis X antigen on high endothelial venules of human lymph nodes: Possible candidate for
L-selectin ligand. Biochem. Biophys. Res. Commun., 193(1): 337-347, 1993.
Takada, A., Ohmori, K., Yoneda, T., Tsuyuoka, K., Hasegawa, A., Kiso, M., and Kannagi, R. Contribution of
carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium.
Cancer Res., 53: 354-361, 1993.
Yago, K., Zenita, K., Ginya, H., Sawada, M., Ohmori, K., Okuma, M., Kannagi, R., and Lowe, J.B. Expression of
α(1,3)fucosyltransferases which synthesize sialyl Lex and sialyl Lea, the carbohydrate ligands for E- and
P-selectins, in human malignant cell lines. Cancer Res., 53: 5559-5565, 1993.
Yago, K., Zenita, K., Ohwaki, I., Harada, R., Nozawa, S., Tsukazaki, K., Iwamori, M., Endo, N., Yasuda, N.,
Okuma, M., and Kannagi, R. Immunoglobulin variable region sequences of two human monoclonal antibodies
directed to an onco-developmental carbohydrate antigen, lactotetraosylceramide (LcOse 4Cer). Mol. Immunol., 30:
1481-1489, 1993.
Furukawa, Y., Tara, M., Ohmori, K., and Kannagi, R. Variant type of sialyl Lewis X antigen expressed on adult T
cell leukemia cells is associated with skin involvement. Cancer Res., 54: 6533-6538, 1994.
Levery, S.B., Salyan, M.E.K., Steele, S.J., Kannagi, R., Dasgupta, S., Chien, J-L., Hogan, E.L., Van Halbeek, H.,
and Hakomori, S. A revised structure for the disialosyl globo-series gangliosides of human erythrocytes and chicken
skeletal muscle. Arch. Biochem. Biophys., 312: 125-134, 1994.
Narita, T., Funahashi, H., Imai, T., Takagi, H., and Kannagi, R. Cytosol and serum concentration of cytokeratin
subunit 19 fragment (CYFRA 21-1) in breast cancer. Oncol. Rep., 1: 747-750, 1994.
Narita, T., Funahashi, H., Kontani, K., and Kannagi, R. NCC-ST-439. Drug. Future, 19(12): 1084-1087, 1994.
Natsuka, S., Gersten, K.M., Zenita, K., Kannagi, R., and Lowe, J.B. Molecular cloning of a cDNA encoding a novel
human leukocyte α1,3-fucosyltransferase capable of synthesizing the sialyl Lewis x determinant. J. Biol. Chem.,
269: 16789-16794, 1994.
Sakamoto, J., Watanabe, T., Kito, T., Yamamura, Y., Kiriyama, K., Kannagi, R., Ueda, R., Takagi, H., and Takahashi,
T. Expression of neural cell adhesion molecule in normal gastric mucosa and in gastric carcinoid tumors. Eur. Surg.
Res., 26: 230-239, 1994.
Yamashita, Y., Chung, Y.S., Sawada, T., Kondo, Y., Hirayama, K., Inui, A., Nakata, B., Okuno, M., Horie, R., Saito,
T., Murayama, K., Kannagi, R., and Sowa, M. A new cancer-associated antigen defined by a monoclonal antibody
against a synthetic carbohydrate antigen. Int. J. Cancer, 58: 349-355, 1994.
Azuma, A., Nishida, T., Yagita, H., Seya, T., Kannagi, R., Okumura, K., Kudoh, S., and Niitani, H. Difference of
complement dependent cytotoxicities against human lung adenocarcinoma cell lines mediated by genetically
rearranged anti-Lewis Y monoclonal antibody. Scand. J. Immunol., 45: 202-208, 1995.
Narita, T., Kawakami, N., Matsuura, N., Hosono, J., and Kannagi, R. Corticosteroids and medroxy-progesterone
acetate inhibit the induction of E-selectin on the vascular endothelium by MDA-MB-231 breast cancer cells.
Anticancer Res., 15: 2523-2528, 1995.
Obama, H., Kaname, T., Sudou, A., Yanagida, T., Ikematsu, S., Ozawa, M., Yoshida, H., Kannagi, R., Yamamura,
K.I., and Muramatsu, T. A transgenic mouse line with α-1,3/4-fucosyltransferase cDNA: Production and
characteristics. Glycoconjugate J., 12: 795-801, 1995.
Suematsu, M., Suzuki, H., Tamatani, T., Iigou, Y., DeLano, F.A., Miyasaka, M., Forrest, M.J., Kannagi, R.,
Zweifach, B.W., Ishimura, Y., and Schumid-Schönbein, G.W. Impairment of selectin-mediated leukocyte adhesion
to venular endothelium in spontaneously hypertensive rats. J. Clin. Invest., 96: 2009-2016, 1995.
Takagi, K., Yasukawa, K., Iba, Y., Isobe, Y., Suketa, Y., Kannagi, R., Kohda, K., Takagi, M., and Imanaka, T.
Expression of recombinant antibody against cancer-specific carbohydrate. J. Ferment. Bioengineer., 79(5):
405-409, 1995.
Tamatani, T., Suematsu, M., Tezuka, K., Hanzawa, N., Tsuji, T., Ishimura, Y., Kannagi, R., Toyoshima, S., and
Homma, M. Recognition of a consensus CHO structure in ligands for selectins by novel antibody against sialyl
Lewis X. Am. J. Physiol. Heart Circ. Physiol., 38: H1282-H1287, 1995.
Page 7
110.
111.
112.
113.
114.
115.
116.
117.
118.
119.
120.
121.
122.
123.
124.
125.
126.
127.
128.
129.
130.
Yamashita, Y., Chung, Y.S., Murayama, K., Kannagi, R., and Sowa, M. Alterations in gastric mucin with malignant
transformation: novel pathway of mucin synthesis. J. Natl. Cancer Inst., 87: 441-446, 1995.
Ye, C., Kiriyama, K., Mitsuoka, C., Kannagi, R., Ito, K., Watanabe, T., Kondo, K., Akiyama, S., and Takagi, H.
Expression of E-selectin on endothelial cells of small veins and proliferating vessels in human colorectal carcinoma.
Int. J. Cancer, 61: 455-460, 1995.
Zhou, J., Iwasa, Y., Konishi, I., Kan, N., Kannagi, R., Kobashi, Y., Kim, Y-C., and Yamabe, H. Papillary serous
adenocarcinoma of the peritoneum in women. A clinico-pathologic and immunohistochemical study. Cancer, 76:
429-436, 1995.
Akamatsu, S., Yazawa, S., Zenita, K., Matsumoto, H., Tachikawa, T., and Kannagi, R. Elevation of an α(1,3)
fucosyltransferase activity correlated with apoptosis of human colon adenocarcinoma cell line, HT-29.
Glycoconjugate J., 13: 1021-1029, 1996.
Hiraiwa, N., Dohi, T., Kawakami-Kimura, N., Yumen, M., Ohmori, K., Maeda, M., and Kannagi, R. Suppression of
sialyl Lewis X expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection
of antisense cDNA of an α
-T VII). J. Biol. Chem., 271: 31556-31561, 1996.
Koike, C., Kannagi, R., Takuma, Y., Akutsu, F., Hayashi, S., Hiraiwa, N., Kadomatsu, K., Muramatsu, T.Y., Nagui,
T., Kobayashi, S., Okada, H., Nakashima, I., Uchida, K., Yokoyama, I., and Takagi, H. Introduction of α
(1,2)-fucosyltransferase and its effect on α-Gal epitopes in transgenic pig. Xenotransplantation, 3: 81-86, 1996.
Nakashio, T., Narita, T., Kimura, N., Akiyama, S., Kasai, Y., Ito, K., Takagi, H., and Kannagi, R. Adhesion
molecules involved in pleural dissemination of esophageal cancer cells. Oncol. Rep., 3: 1063-1066, 1996.
Narita, T., Kawakami-Kimura, N., Kasai, Y., Hosono, J., Nakashio, T., Matsuura, N., Sato, M., and Kannagi, R.
Induction of E-selectin expression on vascular endothelium by digestive system cancer cells. J. Gastroenterol., 31:
299-301, 1996.
Narita, T., Kawakami-Kimura, N., Matsuura, N., Funahashi, H., and Kannagi, R. Adhesion of human breast cancer
cells to vascular endothelium mediated by sialyl Lewisx / E-selectin. Breast Cancer, 3: 19-23, 1996.
Narita, T., Kawakami-Kimura, N., Sato, M., Matsuura, N., Higashiyama, S., Taniguchi, N., and Kannagi, R.
Alteration of integrins by heparin-binding EGF-like growth factor (HB-EGF) in human breast cancer cells.
Oncology, 53: 374-381, 1996.
Sato, M., Narita, T., Kawakami-Kimura, N., Higashiyama, S., Taniguchi, N., Akiyama, S., Hashimoto, T., Manabe,
T., and Kannagi, R. Increased expression of integrins by heparin-binding EGF like growth factor (HB-EGF) in
human esophageal cancer cell. Cancer Lett., 102: 183-191, 1996.
Seko, Y., Enokawa, Y., Tamatani, T., Kannagi, R., Yagita, H., Okumura, K., and Yazaki, Y. Expression of sialyl
LewisX in rat heart with ischemia/reperfusion and reduction of myocardial reperfusion injury by a monoclonal
antibody against sialyl LewisX. J. Pathol., 180: 305-310, 1996.
Seko, Y., Enokawa, Y., Tamatani, T., Kannagi, R., Yagita, H., Okumura, K., and Yazaki, Y. Induction of sialyl
LewisX on the surface of cultured rat vascular endothelial cells and cardiac myocytes by hypoxia/reoxygenation in
vitro. J. Pathol., 180: 300-304, 1996.
Tsuyuoka, K., Yago, K., Hirashima, K., Ando, S., Hanai, N., Saito, H., Yamazaki, M., Takahashi, K., Fukuda, Y.,
Nakao, K., and Kannagi, R. Characterization of a T-Cell line specific to an anti-Id antibody related to the
carbohydrate antigen, sialyl SSEA-1, and the immunodominant T-Cell antigenic site of the antibody. J. Immunol.,
157: 661-669, 1996.
Hiraiwa, N., Hiraiwa, M., and Kannagi, R. Human T-cell leukemia virus-1 encoded Tax protein transactivates α
e Fuc-T VII, which synthesizes sialyl Lewis X, a selectin ligand expressed on adult T-cell
leukemia cells. Biochem. Biophys. Res. Commun., 231: 183-186, 1997.
Nakashio, T., Narita, T., Akiyama, S., Kasai, Y., Fujiwara, M., Ito, K., Takagi, H., and Kannagi, R. Adhesion of
human gastric and pancreatic cancer cells to peritoneal mesothelial cells is mediated by CD44 and β1 integrin. Int.
J. Oncol., 10: 183-188, 1997.
Nakashio, T., Narita, T., Akiyama, S., Kasai, Y., Kondo, K., Ito, K., Takagi, H., and Kannagi, R. Peritoneal
dissemination is inhibited by treatment with antibodies against CD44H, ß1 integrin, and carcinostatic agents in
NUGC-4 human gastric cancer cells. Int. J. Oncol., 10: 355-362, 1997.
Shiga, S., Furukawa, T., Koyanagi, I., Yamagishi, T., Yoshida, Y., Takahashi, T., Kannagi, R., and Mori, T.
Laboratory diagnosis of anemia and related diseases using multivariate analysis. Am. J. Hematol., 54: 108-117,
1997.
Kawakami-Kimura, N., Narita, T., Ohmori, K., Yoneda, T., Matsumoto, K., Nakamura, T., and Kannagi, R.
Involvement of hepatocyte growth factor in increased integrin expression on HepG2 cells triggered by adhesion to
endothelial cells. Br. J. Cancer, 75: 47-53, 1997.
Mitsuoka, C., Kawakami-Kimura, N., Kasugai-Sawada, M., Hiraiwa, N., Toda, K., Ishida, H., Kiso, M., Hasegawa,
A., and Kannagi, R. Sulfated sialyl Lewis X, the putative L-selectin ligand, detected on endothelial cells of high
endothelial venules by a distinct set of anti-sialyl Lewis X antibodies. Biochem. Biophys. Res. Commun., 230:
546-551, 1997.
Nakashio, T., Narita, T., Sato, M., Akiyama, S., Kasai, Y., Fujiwara, M., Ito, K., Takagi, H., and Kannagi, R. The
association of metastasis with the expression of adhesion molecules in cell lines derived from human gastric cancer.
Page 8
131.
132.
133.
134.
135.
136.
137.
138.
139.
140.
141.
142.
143.
144.
145.
146.
147.
148.
149.
150.
151.
Anticancer Res., 17: 293-299, 1997.
Nakashio, T., Narita, T., Akiyama, S., Kasai, Y., Kondo, K., Ito, K., Takagi, H., and Kannagi, R. Adhesion
molecules and TGF-ß1 are involved in the peritoneal dissemination of NUGC-4 human gastric cancer cells. Int. J.
Cancer, 70: 612-618, 1997.
Akahori, T., Yuzawa, Y., Nishikawa, K., Tamatani, T., Kannagi, R., Miyasaka, M., Okada, H., Nigishi, H., and
Matsuo, S. Role of a sialyl Lewisx like epitope selectively expressed on vascular endothelial cells in local skin
inflammation of the rat. J. Immunol., 158: 5384-5392, 1997.
Ito, H., Hiraiwa, N., Sawada-Kasugai, M., Akamatsu, S., Tachikawa, T., Kasai, Y., Akiyama, S., Ito, K., Takagi, H.,
and Kannagi, R. Altered mRNA expression of specific molecular species of fucosyl- and sialyltransferases in
human colorectal cancer tissues. Int. J. Cancer, 71: 556-564, 1997.
Matsuura, N., Narita, T., Mitsuoka, C., Kimura, N., Kannagi, R., Imai, T., Funahashi, H., and Takagi, H. Increased
level of circulating adhesion molecules in the sera of breast cancer patients with distant metastasis. Jpn. J. Clin.
Oncol., 27(3): 135-139, 1997.
Matsuura, N., Narita, T., Mitsuoka, C., Kimura, N., Kannagi, R., Imai, T., Funahashi, H., and Takagi, H. Increased
concentration of soluble E-selectin in the sera of breast cancer patients. Anticancer Res., 17(2B): 1367-1372, 1997.
Narita, T., Kimura, N., Mitsuoka, C., Toi, M., Sato, M., Matsuura, N., Matsumoto, K., Nakamura, T., and Kannagi,
R. Biological and clinical significance of hepatocyte growth factor in breast cancer. Int. J. Oncol., 11: 1305-1310,
1997.
Narita, T., Mitsuoka, C., Kimura, N., Toi, M., Nakashio, T., Sekiguchi, K., Iwanari, H., and Kannagi, R. Increased
concentration of hepatocyte growth factor in the sera of patients with gastrointestinal cancer. Oncol. Rep., 4:
1239-1241, 1997.
Sato, M., Narita, T., Kimura, N., Nakashio, T., Hashimoto, T., Manabe, T., and Kannagi, R. Interaction between
human cancer cells and cultured murine endothelial cells, and its relationship with metastatic potential. Int. J.
Oncol., 10: 1173-1178, 1997.
Sato, M., Narita, T., Kimura, N., Zenita, K., Hashimoto, T., Manabe, T., and Kannagi, R. The association of sialyl
Lewisa antigen with the metastatic potential of human colon cancer cells. Anticancer Res., 17: 3505-3511, 1997.
Wagers, A.J., Stoolman, L.M., Kannagi, R., Craig, R., and Kansas, G.S. Expression of leukocyte fucosyltransferases
regulates binding to E-selectin - Relationship to previously implicated carbohydrate epitopes. J. Immunol., 159:
1917-1929, 1997.
Koike, C., Katayama, A., Kadomatsu, K., Muramatsu, T., Hiraiwa, N., Kannagi, R., Nakashima, I., Yokoyama, I.,
and Takagi, H. Direct gene replacement of the mouse α (1,3)-galactosyltransferase gene with human α
(1,2)-fucosyltransferase gene: converting α-galactose epitopes into H antigens. Xenotransplantation, 4: 147-153,
1997.
Yamashita, Y., Chung, Y.S., Sawada, T., Horie, R., Saito, T., Murayama, K., Kannagi, R., and Sowa, M. F1α: A
novel mucin antigen associated with gastric carcinogenesis. Oncology, 55: 70-76, 1998.
Hosono, J., Narita, T., Kimura, N., Sato, M., Nakashio, T., Kasai, Y., Nonami, T., Nakao, A., Takagi, H., and
Kannagi, R. Involvement of adhesion molecules in metastasis of SW1990, human pancreatic cancer cells. J. Surg.
Oncol., 67: 77-84, 1998.
Matsuura, N., Narita, T., Hiraiwa, N., Hiraiwa, M., Murai, H., Iwase, T., Funahashi, H., Imai, T., Takagi, H., and
Kannagi, R. Gene expression of fucosyl- and sialyl-transferases which synthesize sialyl Lewisx, the carbohydrate
ligands for E-selectin, in human breast cancer. Int. J. Oncol., 12: 1157-1164, 1998.
Mitsuoka, C., Sawada-Kasugai, M., Ando-Furui, K., Izawa, M., Nakanishi, H., Nakamura, S., Ishida, H., Kiso, M.,
and Kannagi, R. Identification of a major carbohydrate capping group of the L-selectin ligand on high endothelial
venules in human lymph nodes as 6-sulfo sialyl Lewis x. J. Biol. Chem., 273: 11225-11233, 1998.
Uchimura, K., Muramatsu, H., Kaname, T., Ogawa, H., Yamakawa, T., Fan, Q.W., Mitsuoka, C., Kannagi, R.,
Habuchi, O., Yokoyama, I., Yamamura, K., Ozaki, T., Nakagawara, A., Kadomatsu, K., and Muramatsu, T. Human
N-acetylglucosamine-6-O-sulfotransferase involved in the biosynthesis of 6-sulfo sialyl Lewis X: Molecular
cloning, chromosomal mapping, and expression in various organs and tumor cells. J. Biochem., 124: 670-678,
1998.
Kumamoto, K., Mitsuoka, C., Izawa, M., Kimura, N., Otsubo, N., Ishida, H., Kiso, M., Yamada, T., Hirohashi, S.,
and Kannagi, R. Specific detection of sialyl Lewis x determinant carried on the mucin GlcNAcß1-6 GalNAc core
structure as tumor-associated antigen. Biochem. Biophys. Res. Commun., 247: 514-517, 1998.
Kurane, S., Krauss, J.C., Watari, E., Kannagi, R., Chang, A.E., and Kudoh, S. Targeted gene transfer for
adenocarcinoma using a combination of tumor-specific antibody and tissue-specific promoter. Jpn. J. Cancer Res.,
89: 1212-1219, 1998.
Narita, T., Kimura, N., Sato, M., Matsuura, N., and Kannagi, R. Altered expression of integrins in
adriamycin-resistant human breast cancer cells. Anticancer Res., 18: 257-262, 1998.
Uchimura, K., Muramatsu, H., Kadomatsu, K., Fan, Q.W., Kurosawa, N., Mitsuoka, C., Kannagi, R., Habuchi, O.,
and Muramatsu, T. Molecular cloning and characterization of an N-acetylglucosamine-6-O-sulfotransferase. J. Biol.
Chem., 273: 22577-22583, 1998.
Mitsuoka, C., Ohmori, K., Kimura, N., Kanamori, A., Komba, S., Ishida, H., Kiso, M., and Kannagi, R. Regulation
Page 9
152.
153.
154.
155.
156.
157.
158.
159.
160.
161.
162.
163.
164.
165.
166.
167.
168.
169.
170.
171.
172.
of selectin binding activity by cyclization of sialic acid moiety of carbohydrate ligands on human leukocytes. Proc.
Natl. Acad. Sci. USA, 96: 1597-1602, 1999.
Fan, Q.W., Uchimura, K., Yuzawa, Y., Matsuo, S., Mitsuoka, C., Kannagi, R., Muramatsu, H., Kadomatsu, K., and
Muramatsu, T. Spatially and temporally regulated expression of N-acetylglucosamine-6-O-sulfotransferase during
mouse embryogenesis. Glycobiology, 9: 947-955, 1999.
Komba, S., Galustian, C., Ishida, H., Feizi, T., Kannagi, R., and Kiso, M. First total synthesis of 6-sulfo
de-N-acetyl-sialyl Lewis x ganglioside: a superior ligand for human L-selectin. Angew. Chem. (Engl), 38:
1131-1133, 1999.
Bistrup, A., Bhakta, S., Lee, J.K., Belov, Y.Y., Gunn, M.D., Zuo, F.-R., Huang, C.-C., Kannagi, R., Rosen, S.D., and
Hemmerich, S. Sulfotransferases of two specificities function in the reconstitution of high-endothelial-cell ligands
for L-selectin. J. Cell Biol., 145: 899-910, 1999.
Kimura, N., Mitsuoka, C., Kanamori, A., Hiraiwa, N., Uchimura, K., Muramatsu, T., Tamatani, T., Kansas, G.S.,
and Kannagi, R. Reconstitution of functional L-selectin ligands on a cultured human endothelial cell line by
co-transfection of α13 fucosyltransferase VII and newly cloned GlcNAcβ: 6-sulfotransferase cDNA. Proc.
Natl. Acad. Sci. USA, 96: 4530-4535, 1999.
Futamura, N., Nakamura, S., Tatematsu, M., Yamamura, Y., Kannagi, R., and Hirose, H. Clinicopathologic
significance of sialyl Lex expression in advanced gastric carcinoma. Br. J. Cancer, 83: 1681-1687, 2000.
Izawa, M., Kumamoto, K., Mitsuoka, C., Kanamori, A., Ohmori, K., Ishida, H., Nakamura, S., Kurata-Miura, K.,
Sasaki, K., Nishi, T., and Kannagi, R. Expression of sialyl 6-sulfo Lewis x is inversely correlated with conventional
sialyl Lewis x expression in human colorectal cancer. Cancer Res., 60: 1410-1416, 2000.
Kontani, K., Taguchi, O., Narita, T., Hiraiwa, N., Sawai, S., Hanaoka, J., Ichinose, M., Tezuka, N., Inoue, S., Fujino,
S., and Kannagi, R. Autologous dendritic cells or cells expressing both B7-1 and MUC1 can rescue tumor-specific
cytotoxic T lymphocytes from MUC1-mediated apoptotic cell death. J. Leukocyte Biol., 68: 225-232, 2000.
Ohmori, K., Kanda, K., Mitsuoka, C., Kanamori, A., Kurata-Miura, K., Sasaki, K., Nishi, T., Tamatani, T., and
Kannagi, R. P- and E-Selectins recognize sialyl 6-sulfo Lewis X, the recently-identified L-selectin ligand. Biochem.
Biophys. Res. Commun., 278: 90-96, 2000.
Sekine, M., Taya, C., Kikkawa, Y., Yonekawa, H., Takenaka, M., Matsuoka, Y., Imai, E., Izawa, M., Kannagi, R.,
and Suzuki, A. Regulation of mouse kidney tubular epithelial cell-specific expression of core 2 GlcNAc transferase.
Eur. J. Biochem., 268: 1129-1135, 2001.
Kontani, K., Taguchi, O., Narita, T., Izawa, M., Hiraiwa, N., Zenita, K., Takeuchi, T., Murai, H., Miura, S., and
Kannagi, R. Modulation of MUC1 mcin as an escape mechanism of breast cancer cells from autologous cytotoxic
T-lymphocytes. Br. J. Cancer, 84: 1258-1264, 2001.
Kumamoto, K., Goto, Y., Sekikawa, K., Takenoshita, S., Ishida, N., Kawakita, M., and Kannagi, R. Increased
expression of UDP-galactose transporter mRNA in human colon cancer tissues and its implication in synthesis of
Thomsen-Friedenreich antigen and sialyl Lewis A/X determinants. Cancer Res., 61: 4620-4627, 2001.
El-Fasakhany, F.M., Uchimura, K., Kannagi, R., and Muramatsu, T. A novel human Gal-3-O-sulfotransferase:
molecular cloning, characterization and its implications in biosynthesis of 3'-sulfo Lewisx. J. Biol. Chem., 276:
26988-26994, 2001.
Ikeda, N., Eguchi, H., Nishihara, S., Narimatsu, H., Kannagi, R., Irimura, T., Ohta, M., Matsuda, H., Taniguchi, N.,
and Honke, K. A remodeling system of the 3'-sulfo Lewis a and 3'-sulfo Lewis x epitopes. J. Biol. Chem., 276:
38588-38594, 2001.
Ito, K., Ye, C.L., Hibi, K., Mitsuoka, C., Kannagi, R., Hidemura, K., Ando, H., Kasai, Y., Akiyama, S., and Nakao,
A. Paired tumor marker of soluble E-selectin and its ligand sialyl Lewis A in colorectal cancer. J. Gastroenterol.,
36: 823-829, 2001.
Sawada, M., Moriya, S., Saito, S., Shineha, R., Satomi, S., Yamori, T., Tsuruo, T., Kannagi, R., and Miyagi, T.
Reduced sialidase expression in highly metastatic variants of mouse colon adenocarcinoma 26 and retardation of
their metastatic ability by sialidase overexpression. Int. J. Cancer, 97: 180-185, 2002.
Uchimura, K., El-Fasakhany, F.M., Hori, M., Hemmerich, S., Blink, S.E., Kansas, G.S., Kanamori, A., Kumamoto,
K., Kannagi, R., and Muramatsu, T. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to
L-selectin ligand synthesis and tumor-associated enzyme expression. J. Biol. Chem., 277: 3979-3984, 2002.
Koike, C., Luppi, P., Sharma, S.B., Kannagi, R., Nakashima, I., Starzl, T.E., and Trucco, M. Molecular basis of
evolutionary loss of
-galactosyltransferase gene in higher primates. J. Biol. Chem., 277: 10114-10120, 2002.
Delcommenne, M., Kannagi, R., and Johnson, P. TNF-a increases the carbohydrate sulfation of CD44: induction of
6-sulfo N-acetyl lactosamine on N- and O-linked glycans. Glycobiology, 12: 613-622, 2002.
Hamada, T., Hirota, H., Yokoyama, S., Otsubo, N., Ishida, H., Kiso, M., Kanamori, A., and Kannagi, R. NMR
analysis of novel ganglioside GM4 analogues containing de-N-acetyl and lactamized sialic acid: probes for
searching new ligand structures for human L-selectin. Magn. Reson. Chem., 40: 517-523, 2002.
Kanamori, A., Kojima, N., Uchimura, K., Muramatsu, T., Tamatani, T., Berndt, M.C., Kansas, G.S., and Kannagi, R.
Distinct sulfation requirements of selectins disclosed using cells which support rolling mediated by all three
selectins under shear flow: J. Biol. Chem., 277: 32578-32586, 2002.
Naruse, T., Yuzawa, Y., Akahori, T., Mizuno, M., Maruyama, S., Kannagi, R., Hotta, N., and Matsuo, S.
Page 10
173.
174.
175.
176.
177.
178.
179.
180.
181.
182.
183.
184.
185.
186.
187.
188.
189.
190.
191.
P-selectin-dependent macrophage migration into the tubulointerstitium in unilateral ureteral obstruction. Kidney
Int., 62: 94-105, 2002.
Takubo, T., Kanashima, H., Terada, Y., Shibata, H., Aoyama, Y., Nakamae, H., Yamamura, R., Shima, M., Makita,
K., Tanaka, K., Ohta, K., Yamane, T., Hino, M., Ohta, T., Hashimoto, S., Kamitani, T., Tatsumi, N., Kumamoto, K.,
and Kannagi, R. Analysis of E-selectin mRNA on leukemia cells: a report on two patients with acute leukemia and
one patient with lymphoma leukemia accompanied by high serum E-selectin levels. Med. Postgraduat., 40:
270-273, 2002.
Schakel, K., Kannagi, R., Kniep, B., Goto, Y., Mitsuoka, C., Zwirner, J., Soruri, A., von Kietzell, M., and Rieber,
E.P. 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human
dendritic cells. Immunity, 17: 289-301, 2002.
Tei, K., Kawakami-Kimura, N., Taguchi, O., Kumamoto, K., Higashiyama, S., Taniguchi, N., Toda, K., Kawata, R.,
Hisa, Y., and Kannagi, R. Roles of cell adhesion molecules in tumor angiogenesis induced by co-transplantation of
cancer and endothelial cells to nude rats. Cancer Res., 62: 6289-6296, 2002.
Hiraiwa, N., Yabuta, T., Yoritomi, K., Hiraiwa, M., Tanaka, Y., Suzuki, T., Yoshida, M., and Kannagi, R.
Transactivation of the fucosyltransferase VII gene by human T-cell leukemia virus type 1 tax through a variant
cAMP-responsive element. Blood, 101: 3615-3621, 2003.
Hamada, T., Hirota, H., Yokoyama, S., Yamaguchi, M., Ohtsu, Y., Ishida, H., Kiso, M., Kanamori, A., and Kannagi,
R. NMR structure elucidation of cyclic sialyl 6-sulfo Lewis x, a biologically dormant form of L-selectin ligand.
Tetrahedron Lett., 44: 1167-1170, 2003.
Tsuchida, A., Okajima, T., Furukawa, Ke., Ando, T., Ishida, H., Yoshida, A., Nakamura, Y., Kannagi, R., Kiso, M.,
and Furukawa, K. Synthesis of disialyl Lewis a structure in colon cancer cell lines by a sialyltransferase
ST6GalNAc VI responsible for the synthesis of a-series gangliosides. J. Biol. Chem., 278: 22787-22794, 2003.
Yamaguchi, M., Ishida, H., Kanamori, A., Kannagi, R., and Kiso, M. Studies on the endogenous L-selectin ligands:
systematic and highly efficient total synthetic routes to lactamized-sialyl 6-O-sulfo Lewis X and other novel
gangliosides containing lactamized neuraminic acid. Carbohydr. Res., 338: 2793-2812, 2003.
Koike, T., Kimura, N., Miyazaki, K., Yabuta, T., Kumamoto, K., Takenoshita, S., Chen, J., Kobayashi, M.,
Hosokawa, M., Taniguchi, A., Kojima, T., Ishida, N., Kawakita, M., Yamamoto, H., Takematsu, H., Kozutsumi, Y.,
Suzuki, A., and Kannagi, R. Hypoxia induces adhesion molecules on cancer cells-a missing link between Warburg
effect and induction of selectin ligand carbohydrates. Proc. Natl. Acad. Sci. USA, 101:8132-8137, 2004.
Miyazaki, K., Ohmori, K., Izawa, M., Koike, T., Kumamoto, K., Furukawa, K., Ando, T., Kiso, M., Yamaji, T.,
Hashimoto, Y., Suzuki, A., Yoshida, A., Takeuchi, M., and Kannagi, R. Loss of disialyl Lewis a, the ligand for
lymphocyte inhibitory receptor Siglec-7, associated with increased sialyl Lewisa expression on human colon
cancers. Cancer Res., 64:4498-4505, 2004.
Murata, K., Miyoshi, E., Ihara, S., Kameyama, M., Ishikawa, O., Doki, Y., Fukuoka, H., Tarui, T., Takada, Y.,
Kannagi, R., Taniguchi, N., and Imaoka, S. Attachment of colon cancer cells onto vascular endothelial cells is
enhanced by N-acetylglucosaminyltransferase V (GnT-V). Oncology, 66:492-501, 2004.
Itano, N., Sawai, T., Atsumi, F., Miyaishi, O., Taniguchi, S., Kannagi, R., Hamaguchi, M., and Kimata, K. Selective
expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant
transformation. J. Biol. Chem., 279:18679-18687, 2004.
Suzuki, A., Yoshioka, S., Sekine, M., Yonekawa, H., Takenaka, M., and Kannagi, R. Core 2 GlcNAc transferase and
kidney tubular cell-specific expression. Glycoconj. J., 20: 151-156, 2004.
Kanda, H., Tanaka, T., Matsumoto, M., Umemoto, E., Ebisuno, Y., Kinoshita, M., Noda, M., Kannagi, R., Hirata, T.,
Murai, T., Fukuda, M., and Miyasaka, M. Endomucin, a sialomucin expressed in high endothelial venules, supports
L-selectin-mediated rolling. Int. Immunol., 16: 1265-1274, 2004.
Yamaguchi, M., Ishida, H., Kanamori, A., Kannagi, R., and Kiso, M. Synthesis and antigenic property of a novel
sialyl 6-O-sulfo Lewis X neo-glycolipid containing lactamized neuraminic acid. J. Carbohydr. Chem., 23: 201-215,
2004.
Hirata, T., Furukawa, Y., Yang, B.G., Hieshima, K., Fukuda, M., Kannagi, R., Yoshie, O., and Miyasaka, M. Human
PSGL-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus.
J. Biol. Chem., 279: 51775-51782, 2004.
Uchimura, K., Kadomatsu, K., El Fasakhany, F.M., Singer, M.S., Izawa, M., Kannagi, R., Takeda, N., Rosen, S.D.,
and Muramatsu, T. N-acetylglucosamine 6-O-sulfotransferase-1 regulates expression of L-selectin ligands and
lymphocyte homing. J. Biol. Chem., 279: 35001-35008, 2004.
Kakizaki, I., Kojima, K., Takagaki, K., Endo, M., Kannagi, R., Ito, M., Maruo, Y., Sato, H., Yasuda, T., Mita, S.,
Kimata, K., and Itano, N. A novel mechanism for the inhibition of hyaluronan biosynthesis by
4-methylumbelliferone. J. Biol. Chem., 279: 33281-33289, 2004.
Tjew, S.L., Brown, K.L., Kannagi, R., and Johnson, P. Expression of N-acetylglucosamine 6-O sulfotransferases
(GlcNAc6STs) -1 and -4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo
N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-α}. Glycobiology, 15: 7C-13C, 2005.
Otsubo, N., Ishida, H., Kannagi, R., and Kiso, M. Design and synthesis of a novel neo-glycolipid containing sialyl
Lewis X determinant carried on the mucin GlcNAcβ1-6GalNAcα core structure. Tetrahedron Asymmetry, 16:
Page 11
192.
193.
194.
195.
196.
197.
198.
199.
200.
201.
202.
203.
204.
205.
206.
207.
208.
209.
1321-1327, 2005.
Satoh, T., Kanai, Y., Wu, M.H., Yokozeki, H., Kannagi, R., Lowe, J.B., and Nishioka, K. Synthesis of α
(1,3)fucosyltransferases IV- and VII-dependent eosinophil selectin ligand and recruitment to the skin. Am. J.
Pathol., 167: 787-796, 2005.
Uchimura, K., Gauguet, J.M., Singer, M.S., Tsay, D., Kannagi, R., Muramatsu, T., Von Andrian, U.H., and Rosen,
S.D. A major class of L-selectin ligands is eliminated in mice deficient in two sulfotransferases expressed in high
endothelial venules. Nat. Immun., 6: 1105-1113, 2005.
Yagi, H., Takahashi, N., Yamaguchi, Y., Kimura, N., Uchimura, K., Kannagi, R., and Kato, K. Development of
structural analysis of sulfated N-glycans by multi-dimensional HPLC mapping methods. Glycobiology, 15:
1051-1060, 2005.
Yamaguchi, M., Ishida, H., Kanamori, A., Kannagi, R., and Kiso, M. 6-O-Sulfo sialylparagloboside and sialyl
Lewis X neo-glycolipids containing lactamized neuraminic acid: Synthesis and antigenic reactivity against G159
monoclonal antibody. Glycoconj. J., 22: 95-108, 2005.
Chen, G.-Y., Osada, H., Santamaria-Babi, L. F., and Kannagi, R. Interaction of GATA-3/T-bet transcription factors
regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes. Proc. Natl. Acad. Sci. USA.,
103: 16894-16899, 2006.
Ohmori, K., Fukui, F., Kiso, M., Imai, T., Yoshie, O., Hasegawa, H., Matsushima, K., and Kannagi, R. Identification
of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis x, a selectin ligand expressed on a subset of
skin-homing helper memory T cells. Blood, 107: 3197-3204, 2006.
Yin, J., Hashimoto, A., Izawa, M., Miyazaki, K., Chen, G.-Y., Takematsu, H., Kozutsumi, Y., Suzuki, A., Furuhata,
K., Cheng, F.-L., Lin, C.-H., Sato, C., Kitajima, K., and Kannagi, R. Hypoxic culture induces expression of sialin, a
sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells.
Cancer Res., 66: 2937-2945, 2006.
Akutagawa, A., Fukami, K., Banno, Y., Takenawa, T., Kannagi, R., Yokoyama, Y., Oda, K., Nagino, M., Nimura, Y.,
Yoshida, S., and Tamiya-Koizumi, K. Disruption of phospholipase Cδ4 gene modulates the liver regeneration in
cooperation with nuclear protein kinase C. J. Biochem., 140: 619-625, 2006.
Kamiyama, S., Sasaki, N., Goda, E., Ui-Tei, K., Saigo, K., Narimatsu, H., Jigami, Y., Kannagi, R., Irimura, T., and
Nishihara, S. Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter,
PAPST2. J. Biol. Chem., 281: 10945-10953, 2006.
Kyogashima, M., Tamiya-Koizumi, K., Ehara, T., Li, G., Hu, R., Hara, A., Aoyama, T., and Kannagi, R. Rapid
demonstration of diversity of sulfatide molecular species from biological materials by MALDI-TOF MS.
Glycobiology, 16: 719-728, 2006.
Biswas, M.H., Hasegawa, H.H., Rahman, M.A., Huang, P., Mon, N.N., Ruhul Amin, A.R., Senga, T., Kannagi, R.,
and Hamaguchi, M. SHP-2-Erk signaling regulates Concanavalin A-dependent production of TIMP-2. Biochem.
Biophys. Res. Commun., 348: 1145-1149, 2006
Kanoh, A., Seko, A., Ideo, H., Yoshida, M., Nomoto, M., Yonezawa, S., Sakamoto, M., Kannagi, R., and Yamashita,
K. Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy- resistant ovarian
adenocarcinomas. Glycoconj. J., 23: 453-460, 2006.
Sobue, S., Iwasaki, T., Sugisaki, C., Nagata, K., Kikuchi, R., Murakami, M., Takagi, A., Kojima, T., Banno, Y.,
Akao, Y., Nozawa, Y., Kannagi, R., Suzuki, M., Abe, A., Naoe, T., and Murate, T. Quantitative RT-PCR analysis of
sphingolipid metabolic enzymes in acute leukemia and myelodysplastic syndromes. Leukemia, 20: 2042-2046,
2006.
Zhuo, L., Kanamori, A., Kannagi, R., Itano, N., Wu, J., Hamaguchi, M., Ishiguro, N., and Kimata, K. SHAP
potentiates the CD44-mediated leukocyte adhesion to the hyaluronan substratum. J. Biol. Chem., 281:
20303-20314, 2006.
Teramoto, K., Kontani, K., Fujita, T., Ozaki, Y., Sawai, S., Tezuka, N., Fujino, S., Itoh, Y., Taguchi, O., Kannagi, R.,
and Ogasawara, K. Successful tumor eradication was achieved by collaboration of augmented cytotoxic activity and
anti-angiogenic effects following therapeutic vaccines containing helper-activating analog-loaded dendritic cells
and tumor antigen DNA. Cancer Immunol. Immunother., 56: 331-342, 2007.
Li, G., Hu, R., Kamijo, Y., Nakajima, T., Aoyama, T., Inoue, T., Node, K., Kannagi, R., Kyogashima, M., and Hara,
A.: Establishment of a quantitative, qualitative, and high-throughput analysis of sulfatides from small amounts of
sera by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Anal. Biochem., 362: 1-7,
2007.
Kontani, K., Teramoto, K., Ozaki, Y., Sawai, S., Tezuka, N., Ishida, H., Kajino, K., Fujino, S., Yamauchi, A.,
Taguchi, O., Kannagi, R., Yokomise, H., and Ogasawara, K.: Preparation of fully activated dendritic cells capable of
priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed
streptococcus pyogenes (OK432) and anti-CD40 antibody. Oncol. Rep., 17: 895-902, 2007.
Koyama, H., Hibi, T., Isogai, Z., Yoneda, M., Fujimori, M., Amano, J., Kawakubo, M., Kannagi, R., Kimata, K.,
Taniguchi, S., and Itano, N.: Hyperproduction of hyaluronan in Neu-induced mammary tumor accelerates
angiogenesis through stromal cell recruitment: Possible involvement of versican/PG-M. Am. J. Pathol., 170:
1086-1099, 2007.
Page 12
210.
211.
212.
213.
214.
215.
216.
217.
218.
219.
220.
221.
222.
223.
224.
225.
226.
227.
228.
229.
230.
231.
Go, S., Sato, C., Yin, J., Kannagi, R., and Kitajima, K. Hypoxia-enhanced expression of free deaminoneuraminic
acid in human cancer cells. Biochem. Biophys. Res. Commun., 357: 537-542, 2007.
Hu, R., Li, G., Kamijo, Y., Aoyama, T., Nakajima, T., Inoue, T., Node, K., Kannagi, R., Kyogashima, M. and Hara,
A.: Serum sulfatides as a novel biomarker for cardiovascular disease in patients with end-stage renal failure.
Glycoconj J, 24:565-571, 2007.
Kimura, N., Ohmori, K., Miyazaki, K., Izawa, M., Matsuzaki, Y., Yasuda, Y., Takematsu, H., Kozutsumi, Y.,
Moriyama, A., and Kannagi, R.: Human B-lymphocytes express  2-6-sialylated 6-sulfo-N-acetyllactosamine
serving as a preferred ligand for CD22/siglec-2. J Biol Chem, 282: 32200-32207, 2007.
Ruhul Amin, AR., Uddin Biswas, MH., Senga, T., Feng, GS., Kannagi, R., Agarwal, ML. and Hamaguchi, M.: A
role for SHPS-1/SIRP in Concanavalin A-dependent production of MMP-9. Genes Cells, 12: 1023-1033, 2007.
Wu, AM., Khoo, KH., Yu, SY., Yang, Z., Kannagi, R. and Watkins, WM.: Glycomic mapping of pseudomucinous
human ovarian cyst glycoproteins: Identification of Lewis and sialyl Lewis glycotopes. Proteomics, 7: 3699-3717,
2007.
Yamasaki, Y., Ito, S., Tsunoda, N., Kokuryo, T., Hara, K., Senga, T., Kannagi, R., Yamamoto, T., Oda, K., Nagino,
M., Nimura, Y. and Hamaguchi, M.: SIRP1 and SIRP2: Their role as tumor suppressors in breast carcinoma cells.
Biochem Biophys Res Commun, 361: 7-13, 2007.
Koyama, H., Kobayashi, N., Harada, M., Takeoka, M., Kawai, Y., Sano, K., Fujimori, M., Amano, J., Ohhashi, T.,
Kannagi, R., Kimata, K., Taniguchi, S., and Itano, N. Significance of tumor-associated stroma in promotion of
intratumoral lymphangiogenesis. Pivotal role of a hyaluronan-rich tumor microenvironment. Am. J. Pathol., 172:
179-193, 2008.
Zhang, H., Yoshioka, S., Miyazaki, M., Kannagi, R., and Suzuki, A. Core 2 GlcNAc modification and megalin
ligand-binding activity. Biochim. Biophys. Acta, 1780: 479-485, 2008.
Nonomura, C., Kikuchi, J., Kiyokawa, N., Ozaki, H., Mitsunaga, K., Ando, H., Kanamori, A., Kannagi, R., Fujimoto,
J., Muroi, K., Furukawa, Y., and Nakamura, M. CD43, but not P-selectin glycoprotein ligand-1, functions as an
E-selectin counter-receptor in human pre-B-cell leukemia NALL-1. Cancer Res., 68: 790-799, 2008.
Lim, K-T., Miyazaki, K., Kimura, N., Izawa, M., and Kannagi, R. Clinical application of functional glycoproteomics
- dissection of glycotopes carried by soluble CD44 variantsin sera of patients with cancers. Proteomics, 8:
3263-3273, 2008.
Chen, G.-Y., Sakuma, K., and Kannagi, R. Significance of NF-B/GATA axis in TNF--induced expression of
6-sulfated cell-recognition glycans in human T-lymphocytes. J. Biol. Chem., 283: 34563-34570, 2008.
Kawamura, Y.I., Toyota, M., Kawashima, R., Hagiwara, T., Suzuki, H., Imai, K., Shinomura, Y., Tokino, T.,
Kannagi, R., and Dohi, T. DNA hypermethylation contributes to incomplete synthesis of carbohydrate determinants
in gastrointestinal cancer. Gastroenterology, 135: 142-151, 2008.
Kyogashima, M., Tadano-Aritomi, K., Aoyama, T., Yusa, A., Goto, Y., Tamiya-Koizumi, K., Ito, H., Murate, T.,
Kannagi, R., and Hara, A. Chemical and apoptotic properties of hydroxy-ceramides containing long-chain bases
with unusual alkyl chain lengths. J. Biochem., 144: 95-106, 2008.
Suzuki, K., Yamamoto, K., Kariya, Y., Maeda, H., Ishimaru, T., Miyaura, S., Fujii, M., Yusa, A., Joo, E.J., Kimata,
K., Kannagi, R., Kim, Y.S., and Kyogashima, M. Generation and characterization of a series of monoclonal
antibodies that specifically recognize [HexA(+/-2S)-GlcNAc]n epitopes in heparan sulfate. Glycoconj. J., 25:
703-712, 2008.
Yaomura, T., Tsuboi, N., Urahama, Y., Hobo, A., Sugimoto, K., Miyoshi, J., Matsuguchi, T., Kannagi, R., Matsuo,
S., and Yuzawa, Y. Serine/threonine kinase, Cot/Tpl2, regulates renal cell apoptosis in ischaemia/reperfusion injury.
Nephrology (Carlton), 13: 397-404, 2008.
Yang, Z., Wu, J.H., Kuo, H.W., Kannagi, R., and Wu, A.M. Expression of sialyl Le x, sialyl Lea, Lex and Ley
glycotopes in secreted human ovarian cyst glycoproteins. Biochimie, 91:423-433, 2009.
Li, G., Hu, R., Kamijo, Y., Nakajima, T., Aoyama, T., Ehara, T., Shigematsu, H., Kannagi, R., Kyogashima, M., and
Hara, A. Kidney dysfunction induced by protein overload nephropathy reduces serum sulfatide levels in mice.
Nephrology (Carlton), 14: 658-662, 2009.
Zhang X, Nakajima T, Kamijo Y, Li G, Hu R, Kannagi R, Kyogashima M, Aoyama T, and Hara A.: Acute kidney
injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside
sulfotransferase in mice, resulting in reduction of liver and serum sulfatides. Biochem Biophys Res Commun,
390:1382-1388, 2009.
Yin, J., Miyazaki, K., Shaner, R.L., Merrill, A.H.J., and Kannagi, R. Altered sphingolipid metabolism induced by
tumor hypoxia - new vistas of glycolipid tumor markers. FEBS Lett., 584: 1872-1878, 2010.
Ebisuno, T., Katagiri, K., Katakai, T., Ueda, Y., Nemoto, T., Inada, H., Nabekura, J., Okada, T., Kannagi, R., Tanaka,
T., Miyasaka, M., Hogg, N., and Kinashi, T. Rap1 controls lymphocyte adhesion cascades and interstitial migration
within lymph nodes in a RAPL-dependent and independent manner. Blood, 15:804-814, 2010.
Inoue, T., Taguchi, I., Abe, S., Li, G., Hu, R., Nakajima, T., Hara, A., Aoyama, T., Kannagi, R., Kyogashima, M. and
Node, K.: Sulfatides are associated with neointimal thickning after vascular injury. Atherosclerosis, 211: 291-296,
2010.
Ueda, M., Shimada, T., Goto, Y., Tei, K., Nakai, S., Hisa, Y., and Kannagi, R.: Expression of CC-chemokine
Page 13
receptor 7 (CCR7) and CXC-chemokine receptor 4 (CXCR4) in head and neck squamous cell carcinoma. Auris
Nasus Larynx, 37: 488-495, 2010.
232. Yusa A, Miyazaki K, Kimura N, Izawa M, Kannagi R: Epigenetic silencing of the sulfate transporter gene DTDST
induces sialyl Lewisx expression and accelerates proliferation of colon cancer cells . Cancer Res, 70: 4064-4073,
2010.
(Reviews and book chapters)
1. Kannagi, R. On activation mechanism of platelet phospholipases. In: E. Usdin, R.T. Borchardt and C.R. Creveling
(eds.), Biochemistry of S-adenosylmethionine and related compounds, pp. 173-185, London: McMillan Publishers
Ltd., 1982.
2. Hakomori, S. and Kannagi, R. Glycosphingolipids as tumor-associated and differentiation markers: A guest editorial. J.
Natl. Cancer Inst., 71: 231-251, 1983.
3. Levery, S.B., Kannagi, R., Nudelman, E., and Hakomori, S. New series of glycolipid structures assessd by 1H-NMR
spectroscopy. In: M.A. Chester, D. Heingaard, A. Lundblad and S. Svensson (eds.), Glycoconjugates, pp. 258-259,
Sweden: Rahms i Lund, 1983.
4. Taki, T., Kawamoto, M., Seto, H., Noro, N., Masuda, T., Kannagi, R., and Matsumoto, M. Alterations of glycolipid
composition and metabolism during the differentiation of mouse myeloid leukemia cells (M1). Gann Monogr. on
Cancer Res., 29: 85-91, 1983.
5. Murachi, T. and Kannagi, R. Calpain and calpastatin: Biochemical and immunohistochemical distribuion studies in
various tissues. In: H. Ogawa (ed.), The biological role of proteinases and their inhibitors in skin, pp. 97-106,
Tokyo: University of Tokyo Press, 1985.
6. Hakomori, S. and Kannagi, R. Carbohydrate antigens in glycolipids and glycoproteins. In: D.M. Weir, L. Herzenberg,
C. Blackwell and L.A. Herzenberg (eds.), Handbook of experimental immunology, Vol.1, Immunochemistry, pp.
9.1-9.39, Boston: Blackwell Scientific Pub. Inc., 1986.
7. Kannagi, R. and Hakomori, S. Monoclonal antibodies directed to carbohydrate antigens. In: D.M. Weir, L. Herzenberg,
C. Blackwell and L.A. Herzenberg (eds.), Handbook of experimental immunology, Vol.4, Applications of
immunological methods in biomedical sciences, pp. 117.1-117.20, Boston: Blackwell Scientific Pub. Inc., 1986.
8. Kannagi, R., Sakihama, T., and Murachi, T. Proposal of the double regulation mechanism for the action of calpain. In:
V. Turk (ed.), Cysteine proteinases and their inhibitors, pp. 339-357, Berlin FRG: Walter de Gruyter & Co., 1986.
9. Kannagi, R., Watanabe, K., and Hakomori, S. Isolation and purification of glycolipid antigens by high-performance
liquid chromatography. Method. Enzymol., 138: 3-12, 1987.
10. Kannagi, R. Carbohydrate autoantigens in autoimmune diseases. In: Y. Hamashima (ed.), Recent advances in
immunopathology, pp. 51-74, Kyoto: Yamashiro Printing Co. Ltd., 1987.
11. Kannagi, R., Yamagata, Y., Ando, Y., Takano, E., Kitahara, A., and Murachi, T. Calpain and calpastatin in human
platelets. Haemostaseologie, 7: 151-157, 1987.
12. Hiraiwa, N., Fukuda, Y., Imura, H., Iida, N., Ishizuka, I., and Kannagi, R. Generation of monoclonal antibodies
directed to sulfolactosylceramide using a human hepatoma cell line, Hep-G2. In: R. Schauer and T. Yamakawa (eds.),
Sialic acids 1988, pp. 198-199, Kiel GmbH FRG: Barbel Mende Verlag, 1988.
13. Kannagi, R., Miyake, M., Zenita, K., Itai, S., Hiraiwa, N., Shigeta, K., Hirashima, K., and Hino, A. Cancer-associated
carbohydrate antigens: Modified blood group substances and onco-developmental antigens on tumor cells. Gann
Monogr. on Cancer Res., 34: 15-28, 1988.
14. Kannagi, R., Hakomori, S., and Imura, H. Cancer-associated serum glycoprotein antigens expressing type 1 and type 2
chain polylactosamines. In: S. Hakomori, C. Reading and D. Marcus (eds.), Altered glycosylation in tumor cells, pp.
279-294, New York: Alan R Liss Inc., 1988.
15. Miyake, M., Ito, M., Hitomi, S., Hino, A., Miyake, N., and Kannagi, R. GM2 ganglioside in human lung cancer studied
with monoclonal antibodies which discriminate N-acetyl and N-glycolyl sialic acid residues. In: R. Schauer and T.
Yamakawa (eds.), Sialic acids 1988, pp. 210-211, Kiel GmbH FRG: Barbel Mende Verlag, 1988.
16. Zenita, K., Hirashima, K., Shigeta, K., Hiraiwa, N., and Kannagi, R. V H gene expression in the monoclonal antibodies
directed to gangliosides having various sialic acid linkages. In: R. Schauer and T. Yamakawa (eds.), Sialic acids
1988, pp. 278-279, Kiel GmbH FRG: Barbel Mende Verlag, 1988.
17. Murachi, T., Adachi, Y., Hatanaka, M., Ishihara, G., Takano, E., Yamagata, Y., and Kannagi, R. Gene expression for
calpain isozymes in human hematopoietic system cells. In: Z-I. Ogita and C.L. Markert (eds.), Isozymes: structure,
function, and use in biology and medicine, (Progress in clinical and biological research, Vol.344), pp. 477-494,
New York: Wiley-Liss Inc., 1990.
18. Kannagi, R. and Magnani, J.L. CD15s (sLex) cluster report. In: S.F. Schlossman, L. Boumsell, W. Gilks, J.M. Harlan,
T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder and R.F. Todd (eds.), Leukocyte
Typing V, pp. 1529-1531, Oxford: Oxford University Press, 1995.
19. Ohmori, K., Yoneda, T., Furukawa, Y., Kannagi, M., and Kannagi, R. Carbohydrate ligands for E-selectin expressed on
Page 14
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
adult T-cell leukaemia cells and their implication in skin infiltration of the leukaemic cells. In: S.F. Schlossman, L.
Boumsell, W. Gilks, J.M. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F.
Tedder and R.F. Todd (eds.), Leukocyte Typing V, pp. 1531-1532, Oxford: Oxford University Press, 1995.
Kannagi, R. Cell adhesion molecules and cancer metastasis: Concerted action of selectins and integrins. In: M.
Tsuchiya, M. Katori and M. Suematsu (eds.), Organ micorcirculation: bridging between basic and clinical sciences,
pp. 37-45, Tokyo: Excerpta Medica Ltd., 1996.
Kannagi, R. Cell adhesion molecules in inflammation. In: T. Oite, F. Shimizu and I. Kihara (eds.), Cell-cell interaction
in kidney, pp. 12-28, Tokyo: Nihon-Igakukan, 1997.
Kannagi, R. Carbohydrate-mediated cell adhesion involved in hematogenous metastasis of cancer. Glycoconjugate J.,
14: 577-584, 1997.
Kannagi, R. CD15 cluster report. In: T. Kishimoto, H. Kikutani, E.G.Kr. von dem Borne, S.M. Goyert, D.Y. Mason, M.
Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura and H. Zola (eds.), Leukocyte Typing VI,
pp. 348-351, New York: Garland Publishing Inc., 1998.
Kannagi, R. CD15s cluster report. In: T. Kishimoto, H. Kikutani, E.G.Kr. von dem Borne, S.M. Goyert, D.Y. Mason,
M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura and H. Zola (eds.), Leukocyte Typing
VI, pp. 352-355, New York: Garland Publishing Inc., 1998.
Kannagi, R. and Kanamori, A. Glycobiology of sialyl 6-sulfo Lewis X, a new carbohydrate ligand for selectins. Trends
Glycosci. Glycotechnol., 11: 329-344, 1999.
Kannagi, R., Mitsuoka, C., Ohmori, K., Kanamori, A., Khoo, K.-H., and Inoue, Y. Cyclic sialic acid: a new regulatory
mechanism of selectin ligand activity. In: Y. Inoue, Y.C. Lee and F.A. Troy (eds.), Sialobiology and Other Novel
Forms of Glycosylation, pp. 37-43, Osaka, Japan: Gakushin Publisher, 1999.
Kannagi, R. Sphingolipid Metabolism and Cell Signaling--Monoclonal anti-glycosphingolipid antibodies. Methods
Enzymol. 312: 160-179, 2000.
Kannagi, R. Transcriptional regulation of expression of carbohydrate ligands for cell adhesion molecules in the
selectin family. Adv. Exp. Med. Biol., 491: 267-278, 2001.
Kannagi, R. and Hakomori, S. Molecular Immunology of Complex Carbohydrates --A guide to monoclonal antibodies
directed to glycotopes. Adv. Exp. Med. Biol., 491: 587-630, 2001.
Kannagi, R. Fucosyltransferase V. In: N. Taniguchi, K. Honke and M. Fukuda (eds.), Handbook of
Glycosyltransferases and Their Related Genes, pp. 232-236, Tokyo: Springer-Verlag, 2001.
Kannagi, R. Fucosyltransferase VI. In: N. Taniguchi, K. Honke and M. Fukuda (eds.), Handbook of
Glycosyltransferases and Their Related Genes, pp. 237-245, Tokyo: Springer-Verlag, 2001.
Ohmori, K., Mitsuoka, C., Kanamori, A., Adachi, K., Kameyama, A., Nonoyama, S., and Kannagi, R. Novel
anti-CD15 antibodies with distinct specificity towards Lewis X determinants carried by mucin core carbohydrates.
In: D.Y. Mason (ed.), Leukocyte Typing VII, pp. 179-182, Oxford: Oxford University Press, 2002.
Kannagi, R., Mitsuoka, C., Kanamori, A., Uchimura, K., Muramatsu, T., Imai, T., Yoshie, O., Matsushima, K., and
Ohmori, K. Expression and selectin-binding activity of sialyl 6-sulfo Lewis X determinant on human helper
memory T lymphocytes. In: D.Y. Mason (ed.), Leukocyte Typing VII, pp. 39-41, Oxford: Oxford University Press,
2002.
Kannagi, R. Use of liposomes containing carbohydrates for production of monoclonal antibodies. Methods Mol.
Biol., 199: 203-218, 2002.
Kannagi, R. Regulatory roles of carbohydrate ligands for selectins in homing of lymphocytes. Curr. Opin. Struct.
Biol., 12: 599-608, 2002.
Kannagi, R. Carbohydrate blood group antigens and tumor antigens. In: S.Y.C. Wong and G. Arsequell (eds.),
Immunobiology of Carbohydrates, pp. 1-33, New York: Kluwer Academic / Plenum Publishers, 2003.
Kannagi, R. Carbohydrate-based treatment of cancer metastasis. In: C.H. Wong (ed.), Carbohydrate-based Drug
Discovery, pp. 803-829, Weinheim, Germany: Wiley-VCH Verlag GmbH & Co., 2003.
Kannagi, R. Carbohydrate recognition systems in cell-cell interactions and signaling. In: Y. Baba (ed.),
Biomolecular Chemistry, A Bridge for the Future, pp. 88-91, Tokyo: Maruzen Co., Ltd., 2003.
Kannagi, R., Goto, Y., and Fukui, F. In search of the carbohydrate structures on CD44 critical for hyaluronic acid
binding - roles of sialylation and sulfation. Trends Glycosci. Glycotechnol., 16: 183-195, 2004.
Kannagi, R., Izawa, M., Koike, T., Miyazaki, K., and Kimura, N. Carbohydrate-mediated cell adhesion in cancer
metastasis and angiogenesis. Cancer Sci., 95: 377-384, 2004.
Kannagi, R. Molecular mechanism for cancer-associated induction of sialyl Lewis X and sialyl Lewis A expression
- the Warburg effect revisited. Glycoconjugate J., 20: 353-364, 2004.
Kannagi, R., Miyazaki, K., Kimura, N., and Yin, J. Selectin-mediated metastasis of tumor cells: Alteration of
carbohydrate-mediated cell-cell interactions in cancers induced by epigenetic silencing of glycogene. In C. Sansom
and O. Markman (eds.), Glycobiology, pp. 274-287. Oxfordshire, UK.: Scion Publishing Ltd., 2007.
Kannagi, R.: Carbohydrate antigen sialyl Lewis a - its pathophysiological significance and induction mechanism in
cancer progression. Chang Gung Med J, 30: 189-209, 2007.
Kannagi, R., Yin, J., Miyazaki, K., and Izawa, M. Current relevance of incomplete synthesis and neo-synthesis for
cancer-associated alteration of carbohydrate determinants. Biochim. Biophys. Acta, 1780: 525-531, 2008.
Page 15
45.
46.
47.
48.
49.
50.
51.
Kannagi, R. and Kimura, N. Monoclonal antibody as a clue to structural analysis of bioactive functional
glycoconjugates. In: N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, K. Kawasaki and S. Hase (eds.), Experimental
Glycoscience, Glycochemistry, pp. 60-63, Tokyo: Springer Japan, 2008.
Kannagi, R. and Seko, A. Roles of carbohydrate-mediated cell adhesion in cancer progression. In: N. Taniguchi, A.
Suzuki, Y. Ito, H. Narimatsu, K. Kawasaki and S. Hase (eds.), Experimental Glycoscience, Glycobiology, pp.
246-251, Tokyo: Springer Japan, 2008.
Kannagi, R. and Ohmori, K. Carbohydrate ligands for selectins in immune cell trafficking. In: N. Taniguchi, A.
Suzuki, Y. Ito, H. Narimatsu, K. Kawasaki and S. Hase (eds.), Experimental Glycoscience, Glycobiology, pp.
148-153, Tokyo: Springer Japan, 2008.
Varki, A., Kannagi, R., and Toole, B.P. Glycosylation changes in cancer. In: A. Varki, R.D. Cummings, J.D. Esko,
H.H. Freeze, G.W. Hart and J.D. Marth (eds.), Essentials of Glycobiology, 2nd edition, pp. 617-632, New York:
Cold Spring Harbor Laboratory Press, 2009.
Kannagi, R., Ohmori, K., and Kimura, N. Anti-oligosaccharide antibodies as tools for studying sulfated
sialoglycoconjugate ligands for siglecs and selectins. Glycoconj. J., 26: 923-928, 2009.
Igarashi, Y. and Kannagi, R. Glycosphingolipids as mediators of phenotypic changes associated with development
and cancer progression. J. Biochem., 47: 3-8, 2010.
Kannagi, R., Sakuma, K., Miyazaki, K., Lim, K T., Yusa, A., Yin, J., and Izawa, M.: Altered expression of glycan
genes in cancers induced by epigenetic silencing and tumor hypoxia: Clues in the ongoing search for new tumor
markers. Cancer Sci., 101: 586-593, 2010.