CURRICULUM VITAE Personal information Name and surname MSc. Taiju Saito Ph.D. Date and country of birth: 1976/10/08 Japan Marital status: Single Address: USB FFPW, Czech Republic Street Zátiši 728/II Postcode: 389 25 City: Vodňany Telephone: 387 774 612 E-mail: tsaito@frov.jcu.cz Web: www.frov.jcu.cz Science and research results 1) Publications on Web of Science (WOS) - 29 2) Sum of times cited without self-citations from WOS – 224 3) Average citations per item from WOS – 7.72 4) h index from WOS - 9 5) The best cited paper – 42 citations: Saito, T., Goto-Kazeto, R., Arai, K., and Yamaha, E., 2008. Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation. Biology of Reproduction 78: 159-166. (IF 2007 = 3.670) 6) The best cited paper where Saito is the first author – 42 citations: Saito, T., Goto-Kazeto, R., Arai, K., and Yamaha, E., 2008. Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation. Biology of Reproduction 78: 159-166. (IF 2007 = 3.670) 7) The highest IF of the published paper: Saito, T., Goto-Kazeto, R., Kawakami, Y., Nomura, K., Tanaka, H., Adachi, S., Arai, K., Yamaha, E., 2011. The mechanism for primordial germ-cell migration is conserved between Japanese eel and zebrafish. PLoS ONE 6: e24460-. (IF 2010 = 4.411) 8) Published 1 methodology; Current Scientific Research Program: Production of offspring via germ-line chimera, development of germ cells and germ plasm transplantation techniques, study of primordial germ cells (PGCs) development, analysis of 1 (4) germ plasm organization in early stage embryo, study of gonadal formation by transplantationbased technique, study of the early embryonic development for aquaculture, development of new techniques for producing sterilized fishes, polyploid breeding and hybrid breeding in teleosts. Education: Doctor of Philosophy (Ph.D., 2004) – Hokkaido University, Graduate School of Fisheries Sciences, specialization: Fisheries Sciences. Master of Science (MSc., 2001) - Tokai University, School of Marine Sciences and Technology, Course of Marine Bioscience, specialization: Science. Work experience in the Czech Republic (total 2 years): 2012 – present: Scientist of CENAKVA CZ.1.05/2.1.00/01.0024, University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters. 2011 – present: Researcher, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany. Work experience in Japan and USA (total 7 years): 2010 – 2011 (2 years): Research fellow, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan. 2008 – 2010 (3 years): Research fellow, Purdue University, West Lafayette, IN, USA. 2004 – 2007 (4 years): Research fellow, Nanae Fresh Water Laboratory, Hokkaido University, Nanae, Japan. 2004 – 2008: Lecture, Municipal Hakodate Nurse’s school, Hakodate, Japan. Experience with the research projects in the Czech Republic within the last ten years 2013 – 2018: co-leader, “Induction of chimerism by transplantation of germ stem cells in critically endangered sturgeons as a tool of their conservation”, 13-26952S. Supported by the Grant Agency of the Czech Republic. 2013 – 2015: co-leader, “Biotechnological approaches in reproduction of freshwater fish”, KON-JAP. Programme CONTACT. Supported by the MEYS. 2011 – present (2014): excellent scientist, “Strethening of excellence scientific teams in FFPW USB”, CZ.1.07/2.3.00/20.0024. Supported by the MEYS. Experience with the research projects supported by European Comimission and USA 2 (4) 2011 – present (2015): co-leader as Czech Republic partner (FFPW USB), “Aquaculture infrastructures for excellence in Europeann fish research”, AQUQEXCEL. Seventh Framework Programme EU, coordinated by dr. Marc Vandeputte, INRA, France. 2008 – 2010: postdoctoral research associate, “Zebrafish ES cell lines for targeted mutagenesis”, supported by the National Institutes of Health (NIH), USA. Experience with other international research projects 2002 – 2004: responsible leader, “Production of germline chimera by transplantation of blastomeres (the original title is in Japanese)”, supported by the Grant-in-Aid for JSPS Fellows, Japan. 2004 – 2006: responsible leader, “Establishment of bioengineering techniques in fish using primordial germ cells (the original title is in Japanese)”, supported by the Grant-in-Aid for Young Scientists (B), Japan. 2007 – 2008: responsible leader, “Surrogate production in fish (the original title is in Japanese)”, Grant-in-Aid for Young Scientists (B), supported by the Grant-in-Aid for Young Scientists (B), Japan. Teaching abroad 2004 – 2008: Teaching of Biochemistry, Municipal Hakodate Nurse’s school, Hokkaido, Japan. Supervisor of PhD. theses 2012 – present: one student, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Czech Republic. Certificates, awards, etc. The most highly cited papers that appeared in the 2007–2008 issues of Biology of Reproduction. Saito, T., Goto-Kazeto, R., Arai, K., Yamaha, E. (2008) Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation. Biol Reprod 78:159-166. Lectures on significant symposia within the last 5 years 2012 Goto, R., Saito, T., Kawakami, Y., Takagi, M., Arai, K., Yamaha, E. Number of primordial germ cells is a key factor in zebrafish ovarian differentiation. Sixth International Symposium on the Biology of Vertebrate Sex Determination. Kona, Hawaii. April 23-27, 2012. 2012 Hou, J., Saito, T., Yamaha, E., Arai, K. Induced androgenetic doubled haploids without irradiation of eggs in loach Misgurnus anguillicaudatus. The International Symposium on Genetics in Aquaculture XI. Auburn University, USA. June 2430, 2012. 3 (4) 2012 Linhartová, Z., Pšenička, M., Kaspar, V., Saito, T., Fujimoto, T., Arai, K., Yamaha, E. Micromanipulations with sturgeon germ cells. AQUA 2012. Prague, Czech Republic. September 2, 2012. 2012 Linhartová, Z., Saito, T., Pšenička, M. Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca). VIth international workshop on biology and culture of the tench (Tinca tinca L.). Pisek, Czech Republic. September 17-20, 2012. 2012 Linhartová, Z., Saito, T., Pšenička, M. Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca). Domestication in Finfish Aquaculture. Olsztyn-Mragowo, Poland. October 23-25. pp. 81. 2012 Pšenička, M., Linhartová, Z., Saito, T. Xenogeneic transplantation of spermatogonia within sturgeons. Domestication in Finfish Aquaculture. Olsztyn-Mragowo, Poland. October 23-25. pp 21. 2012 Saito, T., Linhartova, Z., Arai, K., Yamaha, E., Pšenička M., 2012. A preliminary study on production of germline chimera using transplantation of germ cells in sturgeon. Domestication in Finfish Aquaculture. Olsztyn-Mragowo, Poland. October 23-25. pp 34. 2011 Saito, T., Goto, K., Psenicka, M., Kawakami, Y., Adachi, S., Arai, K., and Yamaha, E. Origin and migration of primordial germ cells in sturgeon. In: The 3rd Internation Workshop on the Biology of Fish Gametes. Budapest, Hungary. Septemebr 7 – 9, 2011. pp 29-30. 2011 Psenicka, M., Saito, T., Goto, K., Adachi, S., Arai, K., and Yamaha, E. Isolation of sturgeon spermatogonia as material for biotechnological applications. The 3rd Internation Workshop on the Biology of Fish Gametes. Budapest, Hungary. Septemebr 7 – 9, 2011. pp 121-122. 2011 Kitauchi-Sekiguchi, T., Saito, T., Motomura, T., Arai, K., and Yamaha, E. Functional primordial germ cells differentiate from egg fragment in goldfish, Carassius auratus. The 3rd Internation Workshop on the Biology of Fish Gametes. Budapest, Hungary. Septemebr 7 – 9, 2011. pp 125-126. 2011 Zhao, Y., Psenicka, M., Fujimoto, T., Saito, T., Yasui, G. S., Yamaha, E., and Arai, K. Diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus. The 3rd Internation Workshop on the Biology of Fish Gametes. Budapest, Hungary. Septemebr 7 – 9, 2011. pp 140-141. 4 (4)