Supplemental Figures
A Subtractively Optimized DNA Microarray Using Nonsequenced Genomic Probes for the Detection of Food-Borne
Pathogens
Jin Yong Lee a,+, Byoung Chan Kim b,+ , Kwan Jong Chang a, Joo-Myung Ahn a, Jee-Hoon
Ryu a, Hyo-Ihl Chang a, and Man Bock Gu a,*
a
School of Life Sciences and Biotechnology, Korea University, Anam-dong,
Seongbuk-gu, Seoul 136-701, Republic of Korea
b
Environment Division, Korea Institute of Science and Technology, Hawolgok-dong,
Seongbuk-gu, Seoul 136-791, Republic of Korea
Supplemental Figure S1. (a) Scanned images of the S. aureus specific hybridization with
different genomic DNA concentrations (0.5, 5, 50, and 100 ng) for random priming. All
hybridization were performed with reference genomic DNA (50 ng each of all three foodborne pathogens) labeled with Cy5 (red) and the test genomic DNA of S. aureus was labeled
with Cy3 (green). Cy3 signals were apparently started to be shown from 50 ng of S. aureus
genomic DNA, while no significant Cy3 signals were observed from 0.5 and 5 ng genomic
DNA. (b) Scatter plot of F635-Cy5 intensity versus F532-Cy3 intensity for the various S.
aureus genomic DNA concentration for random priming. The plot Cy5-F635 vs. Cy3-F532
showed a linear relationship started from 50 ng of the sample DNA (Supplemental Figure
S1b) while the ratio of Cy3/Cy5 has nearly zero below 50 ng.
Supplemental Figure S2. Scanned images of the primary DNA microarray chip at 532 nm
(Cy3) after genomic DNA hybridization for additional seven non-target bacterial genomic
DNAs (Alicyclobacillus acidocaldarius, Alicyclobacillus acidoterrestris, Alicyclobacillus
cycloheptanicus, Escherichia coli O157, Escherichia coli, Listeria monocytogenes, and
Yersinia enterocolitica).
Supplemental Figure S3.
Scanned images at 532 nm (Cy3) of the redesigned DNA
microarrays after the additional seven non-target bacterial genomic DNA hybridization for
Alicyclobacillus
acidocaldarius,
Alicyclobacillus
acidoterrestris,
Alicyclobacillus
cycloheptanicus, Escherichia coli O157, Escherichia coli, Listeria monocytogenes, and
Yersinia enterocolitica.