Dr. Larry R. Masterson, Ph.D.
2615 Park Ave, #611
Minneapolis, MN 55407
lr_masterson@hotmail.com
Home: (612) 554-9180
Cell: (651) 269-6444
EDUCATION
Ph.D., Chemistry (December 2008)
University Of Minnesota, Minneapolis, MN
B.Sc., Chemistry with American Chemical Society Certification (May 2003)
University of Wisconsin- La Crosse, La Crosse, WI
Ph.D. DISSERTATION
“Towards the Full Molecular Details of Protein Kinase A Mediated Catalysis by NMR Spectroscopy.”
Gianluigi Veglia, Advisor, University of Minnesota, Minneapolis, MN
EMPLOYMENT
Postdoctoral Fellow (12/2008-present)
Dept. of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
Research Assistant (4/2004-2008)
Dept. of Chemistry, University Of Minnesota
Structural Biology NMR Laboratory Teacher’s Assistant (6/2005-6/2006)
Dept. of Biochemistry, Molecular Biology, Microbiology and Biophysics, University Of Minnesota
General and Organic Chemistry Teacher’s Assistant (6/2004-5/2005)
Dept. of Chemistry, University Of Minnesota
Quality Assurance Technician (8/2003-12/2004)
Kerry Foods International
Chemistry Teacher’s Aide (6/2001-12/2001, 6/2002-8/2002)
Upward Bound, University of Wisconsin-La Crosse
General/Analytical Chemistry Tutor (8/1999-5/2003)
Dept. of Chemistry, University of Wisconsin-La Crosse
Undergraduate Researcher (8/2000-5/2003)
Dept. of Chemistry, University of Wisconsin-La Crosse
Research Advisor: Adrienne Loh, PhD
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae
HONORS
1998-2002
1999
2000, 2001
2000
2001
2002
2003
2005
2005
2005
2006
2006
2007
2008
2009
2010
2010
2010
UW-L Dean’s List
Leon and Ruth Miller Scholarship for Health Professions
UW-L College of Science and Allied Health Travel Grant for Student Researchers
UW-L Best Student Educational Program of the Year
UW-L Undergraduate Research Grant
Michael J. and Sandra R. Dolan Scholarship in Chemistry
Featured in Who’s Who Among Students at American Universities and Colleges
Outstanding TA of the Year Award
NIH Chemical Biology Training Grant
American Peptide Society Travel Grant
Experimental Nuclear Magnetic Resonance Travel Award
American Heart Association Predoctoral Fellowship
Delegate for the Chemical Biology Interface Training Grant Summit at NIH
Article featured in Chemical and Engineering News, “NMR, mass spectroscopy and
chemical evidence reveal a different chemical structure for methanobactin that
contains oxazolone rings.”
MJ Collins Award for Outstanding Young Innovator in Microwave Chemistry
Cyrus P. Barnum and Anne R. Barnum Postdoctoral Travel Award
Paul Boyer-James Peter Award for Excellence in Postdoctoral Research
NIH-Ruth L. Kirschstein/Minnesota Craniofacial Research Training Fellowship
PEER-REVIEWED PUBLICATIONS
K.H. Ha, L.R. Masterson, Z. Hou, R. Verardi, N. Walsh, G. Veglia, and S.L. Robia.
"Lethal Arg9Cys phospholamban mutation hinders Ca2+-ATPase regulation and
phosphorylation by protein kinase A" (2011) in press in Proc. Natl. Acad. Sci.
L. R. Masterson, C.Y. Cheng, T. Yu, M. Tonelli, A. Kornev, S. S. Taylor, and G. Veglia.
"Dynamics Connect Substrate Recognition to Catalysis in Protein Kinase A" (2010) 6
(11), 821-828, Nature Chemical Biology.
M. Tonelli, L.R. Masterson, G. Cornilescu, J.L Markley, and G. Veglia. “One-Sample
Approach to Determine the Relative Orientations of Proteins in Ternary and Binary
Complexes from Residual Dipolar Coupling Measurements” (2009) 131 (40), 1413814139, JACS.
L.R. Masterson, Shi, L., Tonelli M., Mascioni A., Mueller, M.M., and G. Veglia.
“Backbone NMR resonance assignment of the catalytic subunit of cAMP-dependent
protein kinase A in complex with AMP-PNP” (2009) 3, 115-117, Biomolecular NMR
Assignments.
F. Porcelli, D. Triggiani, B.A. Buck-Koehntop, L.R. Masterson, and G. Veglia.
“Pseudoenzymatic dealkylation of alkyltins by biological dithiols” (2009) 14, 12191225, J Biol Inorg Chem.
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae
L.R. Masterson, N. Bortone, T. Yu, K.N. Ha, E.C. Gaffarogullari, O. Nguyen, and G.
Veglia. “Expression and purification of isotopically labeled peptide inhibitors and
substrates of cAMP-dependant protein kinase A for NMR analysis” (2009) 64, 231236, Protein Expression and Purification.
L.A. Behling, S.C. Hartsel, D.E. Lewis, A.A. DiSpirito, D.W. Choi, L.R. Masterson, G.
Veglia, W.H. “NMR, mass spectroscopy and chemical evidence reveal a different
chemical structure for methanobactin that contains oxazolone rings” (2008), 130,
12604-12605, JACS.
L.R. Masterson, M. Tonelli, J.L. Markley, and G. Veglia. “Simultaneous detection and
deconvolution of congested NMR spectra containing three isotopically labeled species”
(2008) 130, 7818-7819, JACS.
N.J. Traaseth, K. Ha, R. Verardi, L. Shi, J.J. Buffy, L.R. Masterson, and G. Veglia.
“Structural and Dynamic Basis of Phospholamban and Sarcolipin Inhibition of Ca2+ATPase (SERCA)” (2008), 47, 3-13, Biochemistry.
L.R. Masterson, A. Mascioni, N.J. Traaseth, S.S Taylor and G. Veglia. “Allosteric
Cooperativity in Protein Kinase A” (2008), 105, 506-511, Proc. Natl. Acad. Sci.
M. Tonelli, L.R. Masterson, K. Hallenga, G. Veglia, and J. L. Markley. “Carbonyl
Carbon Label Selective (CCLS) 1H-15N HSQC Experiment for Improved Detection of
Backbone 13C-15N Cross Peaks in Larger Proteins” (2007), 29 (3), 177-185, J Biomol
NMR.
L.R. Masterson, M. Etienne, F. Porcelli, G. Barany, R.P. Hammer, and G. Veglia.
“Non-Stereogenic -Aminoisobutryl-Glycyl Dipeptidyl Unit Nucleates Type I’ -Turn in
Linear Peptides in Aqueous solution” (2007), 88 (5), 746-753, Biopolymers.
L.R. Masterson, M.A. Etienne, G. Barany, R.P. Hammer, and G. Veglia, “Impact of a
Single -Disubstituted Amino Acid on -Hairpin Folding” (2006) Proceedings of the
19th American Peptide Symposium, 314.
S. Gazal, L.R. Masterson, and G. Barany, “Facile Solid-Phase Synthesis of C-terminal
Peptide Aldehydes and Hydroxamates from a Common Backbone Amide Linked (BAL)
Intermediate” (2005), 66 (6), J. Pep. Res., 324-332.
L.R. Masterson. “Unlocking the Structure and Flexibility of a Four Residue Peptide
Using 1H NMR Spectroscopy” (2002) UW-La Crosse Journal of Undergraduate
Research. V, 381-389.
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae
ORAL PRESENTATIONS
Educational experiences: From a Small City University to a Large Metropolitan
University. Masterson, LR. University of Wisconsin-Eau Clare, Eau Clare, WI,
November, 2008.
Probing Protein Kinase A Cooperativity, Allostery, and How Phosphorylation Kinetics
Correlate with Dilated Cardiomyopathy.
Biochemistry, Molecular Biology, and
Biophysics Fall 2008 Retreat, Alexandria MN, October, 2008.
Aberrations in the Mechanism of Protein Kinase A Mediated Phosphorylation Caused
by Phospholamban Mutants. Masterson, LR, Taylor, SS, Veglia, G. Biophysical
Society 52nd Annual Meeting, Long Beach, CA, February, 2008.
Intramolecular Signaling in Protein Kinase A as Probed by NMR. Masterson LR,
Veglia, G. University of Minnesota 6th Annual Graduate Student Symposium,
Department of Chemistry, Minneapolis, MN, May, 2007.
Unlocking the Structure and Flexibility of a Four Residue Peptide Using 1H NMR
Spectroscopy. Masterson, LR, Loh, A. Ruhr Universität Bochum Ph.D. Candidate
Seminar, Bochum, Germany, August, 2003.
Unlocking the Structure and Flexibility of a Four Residue Peptide Using 1H NMR
Spectroscopy. Masterson, LR, Loh, A. University of Wisconsin-La Crosse Department
of Chemistry Undergraduate Seminar, May, 2002.
POSTER PRESENTATIONS
NMR reveals backbone dynamics encode functional and inhibitory states in protein
kinase A. L.R. Masterson and G. Veglia. Experimental Nuclear Magnetic Resonance
Conference, Daytona Beach, Florida, April, 2010.
Molecular Details of the Interaction of Protein Kinase A with Phospholamban and
Phospholamban Mutants. L.R. Masterson, T. Yu, L. Shi, and G. Veglia. Protein
Society Annual Meeting, Boston, Massachusetts, July, 2009.
One-Sample Approach for the Structural Determination of Protein-Protein Complexes
by NMR Spectroscopy. L.R. Masterson, T. Yu, L. Shi, and G. Veglia. Experimental
Nuclear Magnetic Resonance Conference, Pacific Grove, California, March, 2009.
Structural and Dynamic NMR Characterization of the Endogenous Inhibitor PKI Free
and Bound to Protein Kinase A. F. Porcelli, L.R. Masterson, T. Yu, S.S. Taylor, L. Shi,
and G. Veglia. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove,
California, March, 2009.
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae
NMR Spectroscopic and Kinetic Investigations of the Interaction of Protein Kinase A
with Phospholamban and Phospholamban Mutants. L.R. Masterson, T. Yu, L. Shi,
and G. Veglia. Institute for Complex Adaptive Matter (ICAM) Meeting, San Diego,
California, December, 2008.
Simultaneous Detection and Deconvolution of Congested Spectra Containing
Isotopically Labeled Enzyme, Substrate, and Inhibitor. L.R. Masterson, M. Tonelli, J.L.
Markley, and G. Veglia. Experimental Nuclear Magnetic Resonance Conference,
Pacific Grove, California, March, 2008.
Abberations in the Mechanism of Protein Kinase A Mediated Phosphorylation Caused
by Phospholamban Mutants. L.R. Masterson and G. Veglia. 52nd Annual Biophysical
Society Meeting, Long Beach, California, February 2008.
Structural Basis for Dilated Cardiomyopathy. L.R. Masterson, S.S. Taylor, and G.
Veglia. Keystone Symposia (Frontiers in Structural Biology), Steamboat, Colorado,
January, 2008.
Intramolecular Signaling in Protein Kinase A as Probed by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Chemical Biology Interface Training Grant Summit, Bethesda,
Maryland, June, 2007.
Intramolecular Signaling in Protein Kinase A as Probed by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Biophysical Society 51st Annual Meeting, Baltimore, Maryland,
March, 2007.
Coupled Motions in Protein Kinase A as Characterized by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Keystone Symposia (Frontiers in Structural Biology), Salt Lake
City, Utah, January, 2007.
Coupled Motions in Protein Kinase A as Characterized by NMR. L.R. Masterson, N.J.
Traaseth, A. Mascioni, S.S. Taylor, and G. Veglia. Experimental Nuclear Magnetic
Resonance Conference, Pacific Grove, California, April, 2006.
Defining Allosteric Regulation in Protein Kinase A. L.R. Masterson, A. Mascioni, N.J.
Traaseth, S.S. Taylor, and G. Veglia. Keystone Symposia (Frontiers in Structural
Biology), Keystone, Colorado, January, 2006.
Impact of a Single -Disubstituted Amino Acid on -Hairpin Folding. L.R. Masterson,
M.A. Etienne, G. Barany, R.P. Hammer, and G. Veglia. 19th American Peptide
Symposium, San Diego, California, June, 2006.
Studying the Structure and Flexibility of a Short 310 –helical Peptide using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. UW-L Celebration of Undergraduate
Research and Creativity, La Crosse, Wisconsin, April, 2002.
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae
Unlocking the Structure and Flexibility in a Series of Peptides Using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. National Conference of Undergraduate
Research- Whitewater, Wisconsin, May, 2002.
Unlocking the Structure and Flexibility in a Series of Peptides Using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. Biophysical Society 46th Annual Meeting,
San Francisco, California, April, 2002.
Studying the Structure and Flexibility of a Short 310 –helical Peptide using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. UW-L Celebration of Undergraduate
Research and Creativity, La Crosse, Wisconsin, April, 2001.
RESEARCH SUPPORT
NIH-Ruth L. Kirschstein/Minnesota Craniofacial Research Postdoctoral Training Fellowship, "
Molecular determinants of cleft palate: understanding the atomic details for protein kinase A
inhibition by secalonic acid" (7/1/09-present)
Goals: Probe the effects and mechanism of PKA inhibition by secalonic acid using
NMR spectroscopy, steady-state kinetics, isothermal titration calorimetry, and
molecular dynamics simulations.
Role: Principle Investigator
American Heart Association Predoctoral Fellowship, “Molecular and Structural Basis of
Dilated Cardiomyopathy: An Atomic Investigation of Key Protein-Protein Interactions” (7/1/066/31/07)
Goals: Solid-phase peptide synthesis of Phospholamban (PLN) analogues and
protein kinase substrates. Solution NMR assignment of C-subunit PKA,
characterization of binding standard ligands, and optimization of kinetic
measurements in the presence of lipids. Characterization of the interactions with
peptide models corresponding to PLN and R9C-PLN.
Role: Principle Investigator
NIH-Chemical Biology Interface Training Grant, “Origins of Dilated Cardiomyopathy: An Atomby Atom Investigation of Dynamic Processes in Key Enzymes” (7/2005-7/2006)
Goals: Solid-phase peptide synthesis of phospholamban analogues and protein
kinase substrates. Optimization of expression and purification for NMR samples of Csubunit PKA.
Role: Trainee
UW-L Undergraduate Research Grant, “Unlocking the Structure and Flexibility of a Series of
Short Peptides Using 1H NMR Spectroscopy” (8/2001-6/2002)
Goals: Solution state peptide synthesis of short 310-helical peptides and use NMR to
characterize the thermodynamic stability of their secondary structure.
Role: Researcher
Dr. Larry R. Masterson, Ph.D.
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Curriculum Vitae