Table A1: Between-species comparison of morphological, tension, bending and cyclic loading/unloading parameters of the four studied macrophyte species Glyceria fluitans, Ranunculus penicillatus, Myriophyllum alterniflorum, and Fontinalis antipyretica with an analysis of variance (ANOVA). For each parameter and species n=30 replicates (i.e. ten stems x three stem parts (bottom, middle and top part)) were used in the ANOVA. * Breaking stress, breaking strain, breaking force and work of fracture for Glyceria fluitans are potential minimum values as stem parts were not broken during the tests due to stem slippage out of the brass tubes holding them in the sampling device. Hence the values were not included in the ANOVA. Table A2: Comparison of morphological, tension, bending and cyclic loading/unloading parameters between the bottom, middle and top parts of the four studied macrophyte species Glyceria fluitans (a), Fontinalis antipyretica (b), Ranunculus penicillatus (c), and Myriophyllum alterniflorum (d) with a variance analysis (ANOVA). For each parameter, species and stem part (bottom, middle and top part) n=10 replicates (i.e. ten stems) were used in the ANOVA. * Breaking stress, breaking strain, breaking force and work of fracture for Glyceria fluitans are potential minimum values as stem parts were not broken during the tests due to stem slippage out of the brass tubes holding them in the sampling device. Hence the values were not included in the ANOVA. Table A1 parameter Diameter unit Mean ± S.D. mm 2 G. fluitans F. antipyretica R. penicillatus M. alterniflorum 4.0 ± 0.5 0.44 ± 0.05 2.4 ± 0.4 1.59 ± 0.19 Cross-sectional area mm 6.8 ± 2.1 0.15 ± 0.04 4.2 ± 1.5 1.8 ± 0.6 ‘tension’ Young’s modulus MN m-2 81 ± 28 379 ± 146 15 ± 6 19 ± 16 Breaking stress kN m-2 4182 ± 1178* 19338 ± 5859 973 ± 277 1820 ± 613 Breaking strain % 11.0 ± 5.5* 14.5 ± 3.7 12.8 ± 3.8 15.9 ± 4.5 Breaking force N 26.6 ± 6.2* 2.9 ± 0.9 3.8 ± 1.2 3.1 ± 0.9 kJ m 311 ± 191* 1815 ± 815 87 ± 38 194 ± 62 modulus MN m-2 90 ± 33 - 12.5 ± 7.1 89 ± 38 Second moment of area mm4 8.4 ± 4.1 - 1.8 ± 0.96 0.30 ± 0.13 Flexural rigidity N mm2 678 ± 277 - 22 ± 14 25 ± 11 Degree of elasticity % 81.9 ± 4.1 71.1 ± 6.5 59.7 ± 6.2 58 ± 6 Energy ratio % 45.5 ± 5.1 32.3 ± 6.3 27.3 ± 7.3 24.6 ± 6.4 35 ± 9.8 250 ± 92 13.0 ± 6.4 29.5 ± 8.1 Work of fracture -3 ‘bending’ Young’s Elastic hysteresis -3 kJ m Table A2 a parameter Diameter Cross-sectional area unit Mean ± S.D. mm 2 mm bottom middle top 4.1± 0.6 4.0 ± 0.6 4.0 ± 0.4 7.4 ± 2.1 7.1 ± 1.9 5.9 ± 2.1 42 ± 13 44 ± 17 49 ± 16 ‘tension’ Young’s modulus G. fluitans MN m-2 -2 Breaking stress kN m 4187 ± 1206* 4121 ± 890* 4240 ± 1490* Breaking strain % 12.5 ± 7.8* 9.8 ± 2.9* 10.9 ± 4.9* Breaking force N 29.2 ± 6.3* 28.1 ± 6.2* 22.5 ± 4.4* Work of fracture kJ m-3 309 ± 208* 311 ± 166* 313 ± 217* modulus MN m-2 94 ± 24 97 ± 30 79 ± 43 Second moment of area mm4 8.3 ± 3.8 9.1 ± 5.1 7.7 ± 3.4 ‘bending’ Young’s 2 Flexural rigidity N mm 733 ± 234 784 ± 283 517 ± 260 Degree of elasticity % 82 ± 3.5 81 ± 5.7 82 ± 2.8 Energy ratio % 45.3 ± 4.1 45.8 ± 6.5 45.3 ± 5.1 Elastic hysteresis kJ m-3 36.4 ± 6.9 35.4 ± 11.6 33.1 ± 10.9 b parameter unit Mean ± S.D. bottom middle top Diameter mm 0.47 ± 0.05 0.42 ± 0.04 0.43 ± 0.05 Cross-sectional area mm2 0.18 ± 0.04 0.14 ± 0.02 0.15 ± 0.04 modulus MN m-2 376 ± 151 453± 152 308 ± 105 Breaking stress kN m-2 18838 ± 4238 20227 ± 7158 18951 ± 6300 Breaking strain % 13.4 ± 3.5 14.3 ± 3.4 15.6 ± 4.3 Breaking force N 3.3 ± 0.8 2.8 ± 1.0 2.8 ± 0.9 kJ m 1598 ± 633 1916 ± 951 1930 ± 868 MN m-2 - - - 4 ‘tension’ Young’s F. antipyretica Work of fracture -3 ‘bending’ Young’s modulus Second moment of area mm - - - Flexural rigidity N mm2 - - - Degree of elasticity % 71.3 ± 5.0 70.5 ± 8.5 71.5 ± 6.2 Energy ratio % 34.3 ± 5.9 31.9 ± 7.9 30.6 ± 4.8 Elastic hysteresis kJ m-3 285 ± 98 240 ± 103 226 ± 68 c parameter unit Diameter mm Cross-sectional area mm2 Mean ± S.D. bottom middle top 2.1 ± 0.3 2.5 ± 0.2 2.7 ± 0.2 2.6 ± 0.5 4.4 ± 1.1 5.6 ± 0.8 ‘tension’ Young’s modulus MN m 16.0 ± 6.3 14.1 ± 6.5 14.3 ± 4.4 Breaking stress kN m-2 1184 ± 226 964 ± 276 771 ± 155 Breaking strain % 12.1 ± 3.5 13.9 ± 4.6 12.6 ± 3.2 R. Breaking force N 3.0 ± 0.6 4.1 ± 1.2 4.4 ± 1.2 penicillatus Work of fracture kJ m-3 96 ± 35 96 ± 45 70 ± 32 -2 ‘bending’ Young’s modulus MN m 11.7 ± 9.8 14 ± 7 11.4 ± 3.6 Second moment of area mm4 0.96 ± 0.5 1.9 ± 0.8 2.5 ± 0.8 -2 2 Flexural rigidity N mm 9.8 ± 8.6 26 ± 15 29 ± 11 Degree of elasticity % 54.5 ± 4.6 61.1 ± 6.6 63.4 ± 3.6 Energy ratio % 26.2 ± 5.9 26.3 ± 8.6 29.6 ± 7.6 17.0 ± 8.0 12.6 ± 5.2 9.5 ± 2.9 Elastic hysteresis -3 kJ m d parameter unit Mean ± S.D. bottom middle top Diameter mm 1.73 ± 0.17 1.61 ± 0.13 1.42 ± 0.12 Cross-sectional area mm2 2.2 ± 0.5 1.9 ± 0.4 1.2 ± 0.3 modulus MN m-2 19.0 ± 7.7 22.1 ± 6.1 16.8 ± 3.4 Breaking stress kN m-2 1711 ± 245 1688 ± 256 2061 ± 993 Breaking strain % 15.4 ± 4.9 14.5 ± 4.7 17.8 ± 3.5 Breaking force N 3.7 ± 0.9 3.2 ± 0.7 2.4 ± 0.7 kJ m 203 ± 47 197 ± 74 182 ± 68 MN m-2 81 ± 32 87 ± 17 100 ± 55 ‘tension’ Young’s M. alterniflorum Work of fracture -3 ‘bending’ Young’s modulus 4 Second moment of area mm 0.42 ± 0.18 0.29 ± 0.11 0.20 ± 0.05 Flexural rigidity N mm2 31 ± 10 25 ± 10 19 ± 9 Degree of elasticity % 58.4 ± 5.7 59.4 ± 6.7 57.1 ± 5.9 Energy ratio % 23.1 ± 5.1 23.0 ± 6.1 27.6 ± 7.3 28.9 ± 7.4 32.3 ± 8.8 27.2 ± 8.0 Elastic hysteresis -3 kJ m