ANATOMICAL TERMS:Term Dorsal Ventral Lateral Anterior Posterior Superficial Proximal Distal Meaning Upper or back surface Under or belly surface Side Toward the front (head) end Toward the back (tail) end Near the surface Close to Far from FETAL PIG DISSECTION SAFETY PRECAUTIONS Extreme care must be taken when using dissection instruments, particularly scalpels. To the extent possible, make cuts away from your body. The pigs are preserved in a chemical solution, so wear gloves, goggles and an apron, and work in a well ventilated area. If chemicals contact your skin, wash them off. When you finish, dispose of the fetal pig in the manner instructed by your teacher. Clean and disinfect all tools, work surfaces and your hands. Page 1 of 9 Circulatory System Photo of the heart with labels of structures studied Vital Statistics: Name Sex Length Age Respiratory System Photo with labels of structures studied Digestive System Photo with labels of structures studied Excretory System Photo with labels of structures studied Page 2 of 9 PROCEDURE: PART 1: EXTERNAL ANATOMY 1. Rinse your specimen and place it on its side in the dissecting tray. 2. Measure the specimen from the snout to the base of the tail. Use the graph below to estimate the gestational age of your specimen. 3. Turn your specimen onto its back. Take a photo of the specimen’s dorsal side to be included in your assignment. 4. Determine the sex of your specimen by comparing its external reproductive organs to those in the diagrams below. 5. Record the name, sex, length and age of your pig for use in your assignment. Page 3 of 9 PART 2: EXPOSING THE ABDOMINAL ORGANS 1. Place the pig with its dorsal surface on the tray. Spread out the limbs. Tie a piece of string to one of the forelimbs near the ankle. Pass the string under the tray and securely tie the other forelimb. Repeat the process with the hind limbs. 2. Select the point just anterior to the umbilical cord on the specimen’s ventral surface. Using forceps pinch the skin of the abdomen along the midventral line and pull it slightly away from the animal. With your scissors, make an incision in the skin. The incision should be just large enough to pass the point of your scissors through. Now make a midventral cut (1) ending just posterior to the midline of the animal. To avoid damaging the organs as you cut, keep the tips of the scissors pointing up. Be careful not to damage the umbilical cord. 3. From the starting point, make a second incision around the base of the umbilical cord extending back to just anterior to the anus (2). 4. Locate the base of the sternum (breast bone), situated in the center of the chest. The ribs are attached to the sternum. Select a point posterior to the sternum and cut across the ventral surface (3). The incision should be posterior to the diaphragm, which you will be able to see as a dome-shaped layer of muscle separating the abdominal and thoracic cavities. 5. Make two final incisions, one on each side of the cuts bordering the umbilical cord and just anterior to the hind limbs (4). Use T pins to pin back the skin to expose the internal organs of the abdominal cavity. The T pins should point away from the specimen so they will not interfere with your work. 6. The organs of the abdomen are covered and protected by a membrane called the peritoneum. The double layered sheets of peritoneum are called mesenteries. Using forceps or a dissecting probe, gently move the mesenteries aside to reveal the underlying organs. 7. Using the diagram to the right, locate and identify the organs of the abdominal cavity. 8. Using your digital camera, take a photo of your fetal pig’s abdominal cavity. Page 4 of 9 PART 3: EXAMINING THE DIGESTIVE SYSTEM 1. Remove the following abdominal organs as carefully and intact as possible. Lay the organs out on a paper towel so they are displayed in the right sequence. Liver Small Intestine Gall Bladder Stomach Duodenum Pancreas Large Intestine 2. Take a photo of the collection of organs displayed on the paper towel to be included in your assignment. Page 5 of 9 PART 4: EXAMINING THE URINARY SYSTEM 1. Remove the following urinary organs as carefully and intact as possible. Lay the organs out on a paper towel so they are displayed in the right sequence. Ureters Kidneys Bladder 2. Take a photo of the collection of organs displayed on the paper towel to be included in your assignment. Kidney Ureter Urinary bladder Page 6 of 9 PART 4: EXAMINING THE ORGANS OF THE THORACIC CAVITY Exposing the organs of the thoracic cavity: 1. Locate the base of the sternum (breast bone), situated in the centre of the chest. The ribs are attached to the sternum. Use this as the starting point for your incision. With forceps, pinch the skin of the abdomen along the midventral line and draw it slightly away form the animal. With your scissors, make an incision on the skin. The incision should be just large enough to pass the point of your scissors through. Now make a midventral cut (shown as #1 in the diagram below). This cut should extend as far forward as the hairs near the base of the throat. Be careful not to damage the underlying body wall as you cut. Remember to keep the tips of your scissors pointing up, not down, to avoid damaging the internal organs. 2. Next, make two cuts (shown as cuts #2 and #3 in the diagram below) from the midventral line in the region of the thoracic cavity. Carefully lift the skin and pin it to the side of the specimen using T pins. The T pins should point away for the specimen so they will not interfere with your work. Page 7 of 9 3. Using a sturdy pair of dissecting scissors, cut the ribs along the sternum, and pry them apart to reveal the organs of the thoracic cavity. 4. Using the forceps or a dissecting probe, remove the connective tissues and membranes that surround the lungs and heart. Examining the Circulatory System: 6. Carefully remove the heart. Cut from the bottom to the top to expose all for chambers as shown in the diagram below and right. Place the heart on a paper towel and take a photo to be used for your assignment. Left and right atria Left and right ventricles Septum Tricuspid valve Bicuspid valve Atrium Ventricle Page 8 of 9 EXAMINING THE RESPIRATORY SYSTEM: 5. Carefully remove the trachea, bronchi and lungs. Ensure that they remain connected together. Place them on a paper towel and take a photo to be used for your assignment. Larynx Trachea Lungs Epiglottis Tongue larynx Trachea Esophagus Lobe of a Lung Page 9 of 9