Cell Biology Webquest Cell Biologists: ______________________________________ ______________________________________ Period: ___________ Date: ____________ Part I: CELL TYPES AND NOT SO CELLULAR VIRUSES Your first assignment is distinguish between eukaryotic and prokaryotic cells and viruses 1 You will need to maneuver around the following sites: http://www.wiley.com/legacy/college/boyer/0470003790/animations/cell_structure/cell_structure.htm and http://www.biology4kids.com/files/cell_main.html. 1. What are the two main types of cells Go to http://www.phschool.com/science/biology_place/biocoach/cells/common.html 2. What are four things in common in all cells? Go to http://www.mcb.uct.ac.za/tutorial/virwhat.html#Viruses 3. What is a virus? 4. List two ways that virus are non-living even though virus are biologically active A single prokaryotic cell can divide several times in an hour. Few eukaryotic cells can divide as quickly. Which of the following statements best explains this difference? A. Eukaryotic cells are smaller than prokaryotic cells. B. Eukaryotic cells have less DNA than prokaryotic cells. C. Eukaryotic cells have more cell walls than prokaryotic cells. D. Eukaryotic cells are more structurally complex than prokaryotic cells. Part II: Macromolecules2 Go to http://www.phschool.com/science/biology_place/biocoach/biokit/intro.html and answer the following questions: 1. What are the six most common elements in biomolecules? 2. Sharing electrons forms what kind of bonds? 3. What is the formula for an amino group_________________? And a phosphate group________? Part III. Cell Organelles Go to http://www.tvdsb.on.ca/westmin/science/sbi3a1/Cells/cells.htm and http://www.phschool.com/science/biology_place/biocoach/cells/intro.html Describe the following structures and functions of the organelles listed: 1 1c. Students know how prokaryotic cells, eukaryotic cells (including those from plants and animals), and viruses differ in complexity and general structure. 2 1h. Students know most macromolecules (polysaccharides, nucleic acids, proteins, lipids) in cells and organisms are synthesized from a small collection of simple precursors. 1. Cell membrane3: 2. What two kinds of molecules are missing and what are their functions – hint starts with a “c” and you will need to recall information presented in class. 3. Cell Wall 4. Vacuole 5. Nucleus 6. Chloroplast 7. Centrioles 8. Endoplasmic Reticulum a. Smooth b. Rough 9. Ribosomes 10. Golgi Apparatus 11. 3 4 Mitochondria4:. 1a. Students know cells are enclosed within semipermeable membranes that regulate their interaction with their surroundings. 1g. Students know the role of the mitochondria in making stored chemical-bond energy available to cells by completing the breakdown of glucose to carbon dioxide. 12. Lysosome Human tears contain the enzyme lysozyme, which damages the cell walls of bacteria. Which of the following statements about lysozyme is most accurate? A. Lysozyme causes mutations in bacterial cell wall molecules. B. Lysozyme is destroyed as it digests bacterial cell wall molecules. C. Lysozyme breaks a specific type of bond in a bacterial cell wall molecule. D. Lysozyme is converted to another chemical by a bacterial cell wall molecule. Part IV: Cell Transport and OSMOSIS Now go to explores osmosis http://www.tvdsb.on.ca/westmin/Science/sbi3a1/Cells/Osmosis.htm and Go to: http://www.wiley.com/legacy/college/boyer/0470003790/animations/cell_structure/cell_structure.htm Click “continue” and answer the “Pop-up Questions.” When you are finished, click on “Plant cell” and read the text. 4) Which organelle in the plant cell would mainly help the cell take in water or get rid of water, just like the potato did? This is also known as “osmosis.” How do you know that this organelle would help with that process? 5) Which organelle, if empty, would cause the plant to wilt? Why is this? Many bacteria live in fresh water. Which of these statements best describes what will happen when freshwater bacterial cells are placed in salt water? a) Water leaves the cell, causing the cell to expand. b) Water leaves the cell, causing the cell to shrink. c) Water enters the cell, causing the cell to expand. d) Water enters the cell, causing the cell to shrink. The diagram below illustrates how plant root cells take in mineral ions from the surrounding soil. Which of the following processes is illustrated? A. active transport B. diffusion C. osmosis D. passive filtration Which of the diagrams below best represents the net movement of molecules in osmosis? A. B. C. D. Part V. NUTRIENT CYCLING Open a new window in your browser and go to http://www.tvdsb.on.ca/westmin/Science/Biology12/Metabolic%20Processes/Metabolic%20Processes.htm and http://www.phschool.com/science/biology_place/biocoach/cellresp/intro.html to explore where the cell respiration process of glycolysis and electron transport chain occur. Make sure to describe what is happening in glycolysis and the electron transport chain, and fermentation A. Glycolysis B. Electron Transport Chain C. Fermentation Part VI. ILLUMINATING PHOTOSYNTHESIS5 Go to http://www.pbs.org/wgbh/nova/methuselah/photosynthesis.html# and complete the following questions. Photosynthesis in plants and a few bacteria is responsible for feeding nearly all life on Earth. Plants and bacteria do this by taking energy from the sun and converting it into a storable form, usually the sugar glucose, which plants use for their own life processes (why they are called producers). Animals that consume plants also make use of this energy, as do those that consume those that consume plants, and so on to the top of the food chain (called consumers). As important a job as making all of the world's food is, there's another vital function that photosynthesis performs. Photosynthesis generates the oxygen that oxygen-breathing animals need to survive. But here we animals repay the favor. We exhale the carbon dioxide that plants need for photosynthesis. This feature takes a look at the oxygen/carbon dioxide cycle and at the process of photosynthesis. The Cycle The girl exhales _______________________. The plant takes in _________________ and releases _______________________. The girl uses the red can to add _________ to the plant. The plant transfers the ____________ throughout the plant. When the blinds come up and _____________ shines on the plant, then the plant actively releases ______________ which the girl then _________. Summary: You added some _________________ along with some __________ and ___________. The plant in the room is now “breathing” and so is the cute little tyke. Atomic Shuffle Photosynthesis, the trapper of ___________________; is needed for life to subsist (continue). Most all plants use the process to make _________ without it most life would desist (discontinue). The process begins with plain _____________ but not from the tap does it flow. Some water is made within leaf cells and some is sucked up from below (roots). The molecular formula for water is __________ which means it has _____ atom of oxygen and _____ atoms of hydrogen. With energy gained from sunlight, the plant strips the _____________ from the _____________ in water. These oxygen atoms then form a twosome (diatomic) and diffuse out of the plant leaf. Meanwhile ________________ has just entered through holes in the leaf called _________________. This gas is exhaled by Earth’s creatures including all invertebrates. The _______________ that enters the plant goes through changes. It’s atoms get rearranged, losing an oxygen which combines with _______ to form water. But the plant has a bigger goal to make food for storage. It builds a big structure made of up (C)_____________, (H)______________, and (O)___________ to form _____________, and it is really sweet. Glucose is a monosaccharide that combines to form starch which is a carbohydrate. The overall photosynthesis reaction is: Since there is water on both sides of the equal sign, the equation can be simplified as: When we balance chemical equations we are demonstrating an important natural law of the conservation of mass. 1) Many land plants store energy in starch. When energy is needed, the starch molecules can be broken down quickly. This chemical reaction produces which of the following? 5 A. amino acids B. lipids C. monosaccharides D. RNA chains 1f. Students know usable energy is captured from sunlight by chloroplasts and is stored through the synthesis of sugar from carbon dioxide Which of the following occurs during photosynthesis? A. CO2 is used to produce water. B. CO2 is absorbed by mitochondria. C. CO2 and H2O are converted to carbohydrates. D. CO2 and H2O are combined into carbonic acid. Part VII. MITOSIS TUTORIAL Go to the following website: http://www.cellsalive.com/mitosis.htm On the left side of the screen is a navigation bar, click on the link to “MITOSIS” Read the text on this page and view the animation, you can slow down the video by clicking step by step through the phases. 1. List the stages of mitosis 2. Which stage does the following occur Chromatin condenses into chromosomes ____________________________ Chromosomes align in center of cell. ____________________________ Longest part of the cell cycle. ____________________________ Nuclear envelope (membrane) breaks down. ____________________________ Cell is cleaved into two new daughter cells. ____________________________ Daughter chromosomes arrive at the poles. ____________________________ Watch the video carefully. 3. The colored chromosomes represent chromatids, each one is an exact duplicate of the other. --How many chromosomes are visible at the beginning of mitosis? -- How many are in each daughter cell at the end of mitosis? The little green T shaped things on the cell are centrioles. -- What happens to the centrioles during mitosis? 4. Identify the stages of these cells: ___________________ ________________ _____________________ _________________ Part VIII. BIOINFOMATICS6 Go to http://www.phschool.com/science/biology_place/biocoach/transcription/intro.html and answer the following questions 1. What is the Central Dogma____________________________________________________ __________________________________________________________________ __________________________________________________________________ 2. Diagram the Central Dogma 3. In What part of the cell does transcription occur _________________________. 4. In what part of the cell does translation occur____________________________. Part IX. LIFE APPLICATIONS Cell Disorders and Diseases… http://www.umdf.org/site/c.dnJEKLNqFoG/b.3041929/ click what is a mitochondrial disease 1. How is a person’s life affected by mitochondrial disease? Go to http://www.pompe.com/patient/learning/pc_eng_pt_lsds.asp 2. What organelle does “Pompe Disease” affect in the cell, and how does this disease affect someone’s life? Games and Activities… http://www.cellsalive.com/puzzles/index.htm and complete jigsaw puzzles! http://www.cellsalive.com/puzzles/index.htm and try the word puzzles at the bottom of the page! http://webinstituteforteachers.org/~halliepeskin/2003/activity3.html plant cell labeling http://webinstituteforteachers.org/~halliepeskin/2003/activity4.html animal cell labeling http://darwin.nmsu.edu/~molbio/cellgame/cellpin.html animal cell labeling http://darwin.nmsu.edu/~molbio/cellgame/CellGamePlant.html plant cell labeling http://science.nhmccd.edu/BIOL/biolab/cell.htm animal cell labeling Congratulations, cell biologists! 6 1d. Students know the central dogma of molecular biology outlines the flow of information from transcription of ribonucleic acid (RNA) in the nucleus to translation of proteins on ribosomes in the cytoplasm. Extra Credit – Challenging Onion Root Tip - Online Activity http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html Read the introduction, then click the “next” button. You will have 36 cells to classify. When you're finished, record your data in the chart below. Interphase Prophase Metaphase Anaphase Telophase Total Number of cells 36 Percent of cells (calculate: number of cells divided by total cells x 100 ) 100 % Did you forget a calculator -- no problem. Go to www.calculator.com and click on the “fractions” calculator. A window with a virtual calculator will open and you can do the math from there. Congratulations, cell biologists!