PRODUCT INFORMATION
HyTra Tissue Protein Extraction Reagent
Product No. W-7851 / 500ml
Introduction
HyTra Tissue Protein Extraction Reagent is ready-to-used reagent for total
protein extraction from tissue samples. The protein extracts can be used for
reporter gene expression assays (β-gal, CAT, alkaline phosphatase),
immunoassays (Western blots, immunoprecipitation, ELISA), kinase assays
(PKC, tyrosine kinase), and phosphatases (general phosphatases, tyrosine
phosphatases). It is compatible with Coomassie Blue and silver staining.
Protein lysates can be used also for DNA-protein interaction assays (gel-shift
assays). Additionally, this reagent can be used with protease inhibitor
(#HC100-007) and/or phosphatase inhibitor (#HC100-008) if necessary.
Storage
This product is stable for one year at 4℃.
Additional Materials required
‧Protease Inhibitor Cocktail (#HC100-007)
‧Phosphatase Inhibitor Cocktail (#HC100-008)
‧Micropipettes and tips
‧Homogenizer
‧Centrifuge tubes
‧4℃ centrifuge
Protocol

Prepare 4℃ centrifuge.

Keep all samples on ice during operation.
1.
If desired, add protease inhibitors and/or phosphatase inhibitor to the
Hytra Tissue Protein Extraction Reagent just before use.
Weigh tissue samples. Use a ratio of ~1 g of tissue to 20 ml Hytra Tissue
Protein Extraction Reagent. Volume of Hytra Tissue Protein Extraction
2.
PRODUCT INFORMATION
3.
4.
5.
Reagent can be adjust if the protein concentration is too low or too high.
Homogenize sample with homogenizer until no obvious particles been
seen. Be aware that the homogenization procedure might be critical for
the functional integrity of the target protein.
Centrifuge the sample at 10,000 × g for 5 minutes to pellet tissue debris.
Collect supernatant and continue with downstream analysis or further
purification.
Note: Lysate preservation requires low temperatures. For long term storage it
is recommended to store the lysate at –70 °C.
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W-7852
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Hytra Cell Protein Extraction Reagent
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100 rxns
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100X Protease Inhibitor Cocktail
100X Phosphatase Inhibitor Cocktail
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