C.P. BIOLOGY Unit 2 - Structure and Function of Living Things Disciplinary Core Idea: From Molecules to Organisms: Structures and Processes OBJECTIVE 1: DEFINE THE TERM BIOLOGY A. Biology is the organized study of living things and their interactions with their natural and physical environments. 1. In biology you will study the origins and history of life and once-living things, how living things interact with one another, and how living things function. This will help you understand how we as humans have a vital role in preserving the natural environment and sustaining life on earth. OBJECTIVE 2: CHARACTERISTICS OF LIVING ORGANISMS A. Life is the quality that distinguishes living things – composed of cells – from nonliving things or those that have died. 1. All living things, all organisms, can be characterized by common aspects of their structure and functioning. 2. Organisms are complex, organized, and built on a hierarchical structure, providing the foundation for the next, from the chemical foundation of elements and atoms, the cells and the systems of individual organisms, to species and populations living and interacting in complex ecosystems. B. All living things possess specific characteristics. An organism is anything that has or once had all of these characteristics. (see page 7) 1. they are organized (arranged in an orderly way) and made of cells (from one to billions). Cells are the basic units of structure and function. 2. they use energy (energy in food is changed into chemical energy by cells). 3. and grow (add mass and in many organisms form new cells and structures). 4. to a specific size and form during development ( the process of natural changes that take place during the life of an organism. 5. they reproduce which is not essential for the survival of the individual but is essential to continue the species(a group of organisms that can breed with one another and produce fertile offspring) 6. respond to stimuli (anything that causes some sort of reaction in the organism) to maintain homeostasis (regulates an organism’s internal conditions to maintain life. 7. they adapt (an inherited characteristic that results from changes to the species over time and in many instances evolve. 8. All living things eventually die OBJECTIVE 3: IDENTIFY THE PARTS OF A CHEMICAL REACTION A. A chemical reaction is the process by which atoms or groups of atoms and substances are reorganized into different substances. It is these chemical reactions that occur inside an organism’s cell that maintain homeostasis and keep the organs and systems functioning. 1. Indications that chemical reactions have taken place include the production of heat or light, and the formation of a gas, liquid or solid. 2. When writing chemical equations, chemical formulas describe the substances in the reaction and arrows indicate the process of change. 3. A chemical reaction shows the reactants, the starting substances, on the left side of the arrow, and the products, what was made during the reaction, on the right side of the arrow. a. ReactantsProducts b. C6H12O6 + O6 CO2 + H20 (Glucose and oxygen react to form carbon dioxide and water) 4. In chemical reactions, matter cannot be created or destroyed (conservation of mass). So, all chemical reactions must show this balance of mass by having equal number of atoms for each element on both sides of a chemical equation. OBJECTIVE 4: RELATE ENERGY CHANGES TO CHEMICAL REACTIONS A. The minimum amount of energy needed for reactants to form products in a chemical reaction is called the activation energy. Some reactions don’t happen because the activation energy is too high. B. Exothermic reactions release energy (heat or light) and the energy of the product is lower than the energy of the reactants. C. Endothermic reactions absorb heat and the energy of the products is higher than the energy of the reactants. 1 D. In every chemical reaction, there is a change in energy due to the making and breaking of chemical bonds as reactants for products. OBJECTIVE 5: SUMMARIZE THE IMPORTANCE OF ENZYMES IN LIVING ORGANISMS A. All living things are chemical factories driven by chemical reactions. In living organisms additional substances must be present to lower the activation energy and allow the reaction to proceed quickly. 1. A catalyst is a substance that lowers the activation energy needed to start a chemical reaction. Catalysts do not increase how much product is made nor are they used up in a reaction. B. Special proteins called enzymes are the biological catalysts that speed up the rate of reactions in biological processes. 1. Enzymes are essential to life. Once used in a chemical reaction, it can be used again because enzymes are catalysts. 2. An enzyme’s name describes what it does and most are specific to one reaction. 3. Substrates, reactants that bind to the enzyme, interact with enzymes at specific places called active sites. a. The substrate and active site have complimentary shapes. b. When the substrate binds to the active site, the active site changes shape and forms the enzyme-substrate complex. This complex helps chemical bonds in the reactants to be broken and new bonds to form. c. pH, temperature and other substances affect enzyme activity. d. Enzymes affect many biological processes; they are the chemical workers of the cells. OBJECTIVE 6: DESCRIBE THE ROLE OF CARBON IN LIVING SYSTEMS A. The element carbon is found in living organisms or in their products. Organic chemistry is the study of organic compounds, those compounds containing carbon. 1. Organic compounds usually also have hydrogen, oxygen and nitrogen. 2. Inorganic compounds: all compounds that are not organic, do not usually contain carbon. Ex. H2O, salts, some acids and bases. An exception is CO2. 3. Chemistry: a carbon atom can form four covalent bonds with other atoms, as a result, organic compounds can be very complex. They can form straight chains, branched chains or rings. B. Most cells store small carbon compounds that will be the building blocks for large molecules called macromolecules. 1. Formed by joining smaller organic molecules together. 2. Polymers are molecules that are made from repeating units of identical or nearly identical compounds called monomers that are linked together by covalent bonds. OBJECTIVE 7: LIST THE FOUR IMPORTANT MACROMOLESCULES, AND DESCRIBE THEIR FUNCTIONS IN LIVING ORGANISMS A. Four important organic molecules found in your body: 1. Carbohydrates 2. Lipids 3. Proteins 4.. Nucleic Acids B. Carbohydrates 1. Defined: compounds of carbon, hydrogen, and oxygen, with two hydrogens for every oxygen (CH2O). 2. Function: main source of energy; can be stored by both plants and animals. In plants the cellulose is used as structural support. 3. Examples: a. Simple: monosaccharides like glucose, fructose and galactose b. Complex: disaccharides like maltose or polysaccharides like starch, glycogen or cellulose. 4. Dehydration synthesis: a reaction in which two molecules are bonded together by the removal of a water molecule. 5. Hydrolysis: the opposite of dehydration synthesis, molecules are broken apart by the addition of a water molecules 2 C. Lipids 1. Examples: fats, oils, and waxes. All have a large proportion (much greater than 2 to1) of C-H bonds and less oxygen than carbohydrates. Ex. C57H110O6 2. Function: a. Cell structure b. Reserve energy supply, in plants it is stored as oils, in mammals it is found under our skin and it cushions and insulates against heat loss 3. Formed: by dehydration synthesis, joining long fatty acid chains to a glycerol molecule. 4. Types: a. Saturated: all carbon bonds in the fatty acid chain are single, usually a solid at room temperature b. Unsaturated: one or more double bonds found in the fatty acid chain, usually a liquid at room temperature. 5. A special kind of lipid, called a phospholipid, is responsible for the structure and function of the cell membrane. Lipids are hydrophobic, they do not dissolve in water. This lets lipids serve as barriers in biological membranes. 6. Steroids are a group of lipids that include substances like cholesterol and hormones. Cholesterol is the starting point in the formation of vitamin D and the hormones estrogen and testosterone. a. Cholesterol can be a problem if it builds up in the artery walls, can lead to heart attacks and strokes b. Saturated fats found in butter and meat can increase cholesterol C. Proteins 1. Contain: nitrogen plus carbon, hydrogen and oxygen but can vary greatly in their structure 2. Functions: a. Structural: cells and body tissues like hair, nails, cartilage and connective tissue b. Pigments: blood, skin, eyes, chlorophyll c. Hormones: chemical messengers d. Contractile materials of muscles e. Antibodies: immune system f. Transport molecules g. Enzymes: used during reactions D. Amino Acids: structural building blocks of proteins 1. Peptide Bonds a. Formed by dehydration synthesis 2. Polypeptide: chain of amino acids held together by peptide bonds 3. Protein: a polypeptide chain(s) 4. Protein shapes: coils, sheets, and globules E. Nucleic Acids 1. Two types: DNA and RNA 2. Both types are made of nucleotides. A nucleotide consists of a base, a sugar and a phosphate molecule all joined together. 3. DNA a. DNA structure: two chains of nucleotides in the shape of a double helix (1) Possible bases: adenine, thymine, guanine and cytosine (2) Sugar: deoxyribose (3) Phosphate molecule b. Purpose: hereditary material transmitted from one generation to the next during reproduction, it directs and controls the cell 4. RNA a. RNA structure: a single chain of nucleotides (1) Possible bases: adenine, uracil, guanine, and cytosine (2) Sugar: ribose (3) Phosphate molecule b. Purpose: to copy the DNA code and take it out into the cell to direct protein synthesis 3 5. Summary Sugar Base difference # of chains types DNA Deoxyribose Thymine Double 1 RNA Ribose Uracil Single 3 OBJECTIVE 8: DIFFERENTIATE BETWEEN A PROKARYOTE AND A EUKARYOTE CELL A. Cells differ in their shapes and sizes and vary in their functions that they perform. Organisms and their parts are made of cells which are structural units of life and which themselves have a molecular substructures that support their functioning.. B. Organisms range from a single cell (unicellular) to multi-celled organism. Cells are grouped into two broad categories, prokaryotic and eukaryotic cells. 1. Eukaryotic cells contain a nucleus and other membrane bound organelles a. Occur mostly in multicellular organisms b. Organelles allow many chemical reactions to occur at the same time although in different locations in the cell. 2. Prokaryotic cells lack internal membrane bound structures. a. Mostly occur in bacteria (unicellular) so smaller than eukaryotic cells b. May be similar to first organisms on Earth C. Specialized structures within cells are responsible for specific cellular functions. These essential functions of a cell of a cell involve chemical reactions between many types of molecules, including water, proteins, carbohydrates, lipids, and nucleic acids. D. Within multi-celled organisms, different groups of large numbers of cells work together to form systems of tissues and organs that are specialized for particular functions. OBJECTIVE 9: DESCRIBE HOW A CELL’S PLASMA MEMBRANE FUNCTIONS A. All cells have a plasma membrane, which is a special boundary that controls what moves into and out of a cell. B. The survival of a cell depends on its ability to maintain the proper conditions within itself (homeostasis). 1. Fundamental life processes of plants and animals depend on a variety of chemical reactions that occur in specialized areas of the organisms cell. C. The job of the plasma membrane, the boundary between the cell and its environment, is to allow a steady supply of nutrients to come into the cell and to remove the excess and waste products. D. The plasma membrane is selectively permeable – it allows some molecules into the cell (water, sugars, oxygen) while keeping others out (bacteria). (See page 187) E. The structure of the plasma membrane is a phospholipid bilayer , in which two layers of phospholipids are arranged tail to tail along with proteins on its surface or embedded in the membrane.( see page 188 Fig. 7.6) F. The structure of the plasma membrane is a phospholipid bilayer , in which two layers of phospholipids are arranged tail to tail along with proteins on its surface or embedded in the membrane.( see page 188 Fig. 7.6) 1. Two layers of lipid molecules are organized with the polar heads of the molecules forming the outside of the membrane and the nonpolar tails forming the inside of the membrane a. Water soluble substances will not move easily through the plasma membrane because they are stopped by the nonpolar middle, allowing the cell to separate the environment inside the cell from the environment outside the cell. 2. Proteins are a component of the plasma membrane. a. Proteins that span the entire membrane are transport proteins, regulating molecules that b. Proteins on the outside of the cell act as receptors and transmit signals to the inside of the cell. c. Proteins on the inner surface anchor the plasma membrane to the cell’s internal support structure, giving the cell its shape. 3. Cholesterol molecules helps to keep the fatty acid tails of the phospholipids from sticking together so it is needed to maintain the structure of the plasma membrane and maintain homeostasis. 4 4. Carbohydrates stick out of the plasma membrane help cells identify each other. E. The fluid mosaic model refers to a plasma membrane with substances that can move around within the membrane. OBJECTIVE 10: LIST THE STRUCTURES OF A TYPICAL EUKARYOTIC CELL AND THEIR FUNCTIONS A. Each component of a eukaryotic cell has a specific job, and all the parts of the cell work together to help the cell survive. (See summary of cell structures on page 199) B. Some organelles act as specialized boundaries 1. Plasma membrane – selectively permeable. Controls movement into and out of the cell. 2. Cell wall – found in plants, fungi, and most bacteria. Located outside the plasma membrane and provides additional support and locomotion. Made of cellulose. C. Some organelles are involved in cell control and protein synthesis 1. Nucleus – leader or director of the cell. Contains DNA. Surrounded by a porous membrane. 2. Nucleolus – found within the nucleus and makes ribosomes 3. Chromatin/chromosomes – chromatin is threads of DNA that can be wound on protein spools to form rod-shaped chromosomes. 4. Ribosomes – located outside of the nucleus in the cytoplasm; site where the cell assembles enzymes and other proteins according to the directions of the DNA. 5. Cytoplasm – clear, gelatinous fluid inside the cell. D. Some organelles assemble, transport, and store molecules 1. Endoplasmic Reticulum – site of cellular chemical reactions. A complex folded membrane that forms a transport system between the nucleus and the cytoplasm. May be rough (has ribosomes attached; exports synthesized proteins to golgi apparatus) or smooth (no ribosomes attached, acts to modify or detoxify lipids). 2. Golgi Apparatus – flattened system of tubular membranes that sort the proteins into packages to be sent to the appropriate destination. 3. Vacuoles – membrane bound space for temporary storage of materials. 4. Lysosomes – contain digestive enzymes E. Some organelles provide the cell with energy 1. Chloroplasts – contain chlorophyll, a pigment that traps light energy to produce food that is stored until a later time. 2. Mitochondria – break down food molecules to release energy that powers most cell activities F. Some organelles provide support and locomotion for the cell. 1. Cytoskeleton – acts as a scaffold to provide support for the organelles suspended within the cytoplasm 2. Cilia – short, numerous, hair-like projections that move in a wave-like motion to propel a cell or aid in feeding. 3. Flagella – long, whip-like projections for movement of the cell. OBJECTIVE 11: COMPARE AND CONTRAST THE STRUCTURES OF A PLANT AND ANIMAL CELL A. Plant and animal cells have many of the same organelles as they both need to perform the same critical functions. (survive, grow, and reproduce). B. Pant and animal cells do differ in some ways 1. Animal cells have centrioles (used in cellular reproduction) 2. Animal cells usually have many small vacuoles; plant cells usually have on large vacuole 3. Plant cells in general are larger than animal cells. 4. Plants cells have a cell wall( for protection and support) and chloroplasts which contain chlorophyll. OBJECTIVE 12: EXPLAIN THE PROCESS AND IMPORTANCE OF DIFFUSION AND PREDICT THE EFFECT OF A HYPOTONIC, HYPERTONIC AND ISOTONIC SOLUTION OF A CELL A. Diffusion - Movement of molecules from a higher concentration to a lower concentration 1. Movement will occur if there is a concentration gradient (difference) 2. Movement will continue but eventually there will be no overall change. At this point dynamic equilibrium has been reached. B. Movement of water, lipids and lipid soluble substances are some of the compounds that can pass through the plasma membrane. Movement may or may not require energy 5 C. Passive transport: no energy required, just diffusion. Facilitated diffusion uses channel and carrier proteins, driven by concentration gradient trying to reach equilibrium D. Osmosis 1. The diffusion of water across a semipermeable membrane. Water will move across a membrane trying to reach equilibrium (the amount of water being equal on both sides). Water will diffuse toward the side with the greater concentration of dissolved particles. 2. Effects of osmosis: a. Isotonic: water is in equilibrium, no net movement, cell retains .normal shape b. Hypertonic: high concentration of dissolved substances, low amounts of water, water will move in, cell will swell. c. Hypotonic: low concentration of dissolved substances, higher amounts of water, water will move out, cell will shrink Example: a membrane separates two solutions, one solution is seawater, and the other is fresh. The seawater is hypertonic to the freshwater, or the freshwater is hypotonic to the seawater. Which way will the water move? OBJECTIVE 13: EXPLAIN THE PROCESS OF ACTIVE TRANSPORT AND THE TRANSPORT OF LARGE MOLECULES A. Active transport: energy required as molecules move against a concentration gradient. Uses carrier proteins that bind to the substance. (see fig. 7.26 page 205) 1. Carrier proteins change shape, release substances on the other side of the membrane, then are restored to its original shape. 2. A common active transport pump in the plasma membrane of animal cells is the sodiumpotassium ATPase pump. a. Controls the levels of Na+ and K+ inside cells. b. Allows other substances to “piggy back” their way into or out of a cell by coupling with another substance that uses an active transport pump. (see page 206.) B. Some substances are too big to move through the plasma membrane by diffusion or transport proteins. 1. Exocytosis: cell membrane expels substances (wastes, secrete hormones) 2. Endocytosis: cell membrane engulfs substances, pinocytosis (cell drinking) or phagocytosis (cell eating) (See fig. 7.29 page 207). OBJECTIVE 14: Relate DNA to Protein Synthesis and Cellular Activities A. All cells contain genetic information in the form of DNA. B. Genes are specific regions within the extremely large DNA molecules that form chromosomes. 1. Genes contain the instructions that code for the information of molecules called proteins, which carry out most of the work of cells to perform the essential functions of life. a. Proteins provide structural components, serve as signaling devices, regulate cell activities, and determine the performance of cells through their enzyme activities. OBJECTIVE 15: SUMMMARIZE THE NEGATIVE FEEDBACK MECHANISM USING HORMONE LEVELS IN THE BODY AS AN EXAMPLE A. B. The amount of hormone released from a gland is determined by your body’s demand for that hormone at a given time. Ensures that the right amount is in your system at all times. The endocrine system is controlled by negative feedback system, a system in which the hormones, or their effects, are fed back to inhibit the original signal 1. If hormone level is too low the glands will start secreting, if the level of hormone is too high, the gland will stop secreting. 2. Example is regulation of blood glucose a. blood: normal glucose levels b. eat: glucose levels rise in the blood c. pancreas begins secreting insulin into the bloodstream d. Insulin causes liver and other tissues to take up more glucose e. Homeostasis restored, return to normal glucose levels in the blood. 3. 6 a. A and B represent two related metabolic processes. An increase in A causes an increase in B. However, the change in B also causes a change in A. This is called feedback. If the change caused by B is opposite to the original change in A, the feedback is called negative. Negative feedback prevents A from changing very much in either direction and helps to maintain homeostasis. SUMMARY: STRUCTURE AND FUNCTION A. Systems of specialized cells within organisms help them perform the essential functions of life, Which involve chemical reactions that take place between different types of molecules, such as water, proteins, carbohydrates, lipids and nucleic acids. B. Genes are different regions in the DNA that contain instructions that code for the formation of proteins, which carry out most of the work of cells. C. Multicellular organisms that a hierarchical structure organization, in which one system is made up of numerous parts and itself a component of the net level. D. Feedback mechanisms maintain living system’s internal conditions within certain limits and Mediate behaviors, allowing it to remain alive and functioning even as external conditions change within some range. 1. Outside that range (ex. Too high or too low a temperature, with too little food or water available), the organisms cannot survive. 2. Feedback mechanism can encourage (through positive feedback) what is going on in the living system. 7