CURRICULUM VITAE
NAME:
Jian WANG
ACADEMIC QUALIFICATIONS:
1993
PhD, Study of Cytokinin Metabolism in Relation to Plant Development. Research School of
Biological Sciences, The Australian National University, under the supervision of Professor D.S. Letham.
1982
BSc, Department of Biology, Hebei Teacher's University.
PROFESSIONAL EXPERIENCE:
2008 -present
Senior Technical Officer, CSIRO Ecosystem Sciences, Australia.
2002 -2007
Research Fellow, Plant Cell Biology Group, Research School of Biological Science, The
Australian National University. Supervisor, Professor Richard Williamson
Study of the roles of different CesA (cellulose synthase) genes in cellulose production in Arabidopsi. I
constructed chimeric CesA genes and tested their ability to complement specific CesA mutants. The
domain-swapping experiments have provided an improved understanding of the process of cellulose
biosynthesis at the molecular level. The results are published in Plant Physiology and the founding is high
lighted as of outstanding interest (see Current Opinion in Plant Biology 2007, 10:220-226). Specific CesA
antibodies have also been produced by production of recombinant, his-tagged, proteins. Affinity purified
antibodies are proved to be specific to three different CesAs and have been used to analyse membrane
bound cellulose synthase complex in Arabidopsis. Co-immuno-precipitation of the three CesAs and BNPAGE (blue native polyacrylamide gel electrophoresis) have elucidated, at least partly, the structure and
component of the cellulose synthase complex of the primary cell wall. The antibodies are also used to
analyse the cellular localisation and expression of these CesA proteins. A manuscript has been submitted
for publication.
1993 - 2002
Post-Doc and Research Officer, John Curtin School of Medical Research and Research School of
Biological Sciences, the Australian National University.
Isolation, characterisation and manipulation of plant vacuolar protease genes. Chromosome landing: cloning
and analysis of a plant cell division orientation determination (dod1) gene from Arabidopsis. Worked on a
cancer immunotherapy project in collaboration with Dr. Paul Craft in The Canberra Hospital.
PUBLICATIONS:
1.
J. Wang, D.S. Letham, E. Taverner, J. Badenoch-Jones and C.H. Hocart. (1995) A
procedure for quantification of cytokinins as free bases involving scintillation proximity
immunoassay. Physiol. Plant. 95, 91-98.
2.
J. Wang and D.S. Letham. (1995) Cytokinin oxidase - purification by an affinity column
and activation by caffeic acid. Plant Science. 112, 161-166.
3.
J. Wang, D.S. Letham, E. Cornish and K.R. Stevenson. (1997) Studies of cytokinin
action and metabolism using tobacco plants expressing ipt and GUS genes controlled by a
chalcone synthase promoter. I. Development of the transgenic plants. Australian Journal of
Plant Physiology. 5, 661-672.
4.
J. Wang, D.S. Letham, E. Cornish, K. Wei, C.H. Hocart, M. Michael and K.R.
Stevenson. (1997) Studies of cytokinin action and metabolism using tobacco plants expressing
ipt and GUS genes controlled by a chalcone synthase promoter. II. ipt and GUS gene expression,
cytokinin levels and metabolism. Australian Journal of Plant Physiology. 5, 673-683.
5.
J. Wang, G. Dean Price and Peter C. L. John. (1997) Nucleotide sequence of a cDNA
clone for a glycolate oxidase from Arabidopsis thaliana. Plant Physiology. 115, 315.
6.
J. Wang, Charles H Hocart, Ke-jun Wei and Peter C. L. John. (1998) Nucleotide
sequence of a cDNA clone containing tandem ankyrin repeats and a glycosyl hydrolase family I
signature from Arabidopsis thaliana. Plant Physiology. 117, 1526.
7.
E. Taverner, D.S. Letham, J. Wang, E. Cornish, D.A. Willcocks (1999) Influence of
ethylene on cytokinin metabolism in relation to Petunia corolla senescence. Phytochemistry. 51,
341-347.
8.
J. Wang, P. Howles, A. Cork, R. Birch, R.E. Williamson. (2006) Chimeric proteins
suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their
specific sites in the cellulose synthase of primary walls. Plant Physiology. 142, 685-695.
9.
J. Wang, Janet Elliott and Richard E Williamson. (2008) Features of the primary wall
CESA complex in wild type and cellulose-deficient mutants of Arabidopsis thaliana. Journal of
Experimental Botany. 59, 2627-2637.
10.
H. Dacres, J. Wang, MM.Dumancic and SC.Trowell. (2010) Experimental
determination of the forster distance for two commonly used bioluminescent resonance
energy transfer pairs. Analytical Chemistry 82: 432-435.
15.
REFEREES:
Prof. Richard. E. Williamson,
Plant Cell Biology Group,
Research School of Biological Sciences,
The Australian National University,
GPO.Box 475,
Canberra ACT 2601,
Australia.
Phone +61 2 6125 5087
Fax
+61 2 6125 4331
Email: richard.williamson@anu.edu.au
Dr. Charles. H. Hocart,
Genomics Interaction Group,
Research School of Biological Sciences,
The Australian National University,
G.PO.Box 475,
Canberra ACT 2601,
Australia.
Phone +61 2 6125 5071
Fax
+61 2 6125 4331
Email: Charles.Hocart@anu.edu.au
Dr. Peter.C. L. John,
Plant Cell Biology Group,
Research School of Biological Sciences,
The Australian National University,
P.O.Box 475,
Canberra ACT 0200,
Australia.
Phone 61 2 6125 4584
Fax
61 2 6125 4331
Email: peter.john@anu.edu.au