Hong Lin
Crop Diseases, Pests and Genetics
Research Plant Physiologist
hong.lin@ars.usda.gov
Phone: (559) 596-2933
Fax: (559) 596-2921
9611 S. RIVERBEND AVE.
PARLIER, CA, 93648
Projects
Epidemiology and Management of Xylella Fastidiosa (Xf) and Other Exotic and Invasive Diseases and Insect
Pests
Appropriated (D)
Accession Number: 411381
Development of An Integrated Research Plan for Management of Zebra Chip in Potatoes
Reimbursable (R)
Accession Number: 418836
Molecular Characterization and Functional Determination of Pd Resistance Genes in Grapes
Specific Cooperative Agreement (S)
Accession Number: 416926
Whole Genome Sequencing and Annotation of a New Species Candidatus Liberibacter Associated with
Potato Zebra Chip (Zc) Disease
Specific Cooperative Agreement (S)
Accession Number: 416898
Grape Metabolomic Profiles and Natural Defenses Against Biotic Stress
Specific Cooperative Agreement (S)
Accession Number: 410344
Metabolomic Characterization of ¿candidatus Liberibacter¿ Associated Citrus Huanglongbing and Potato
Zebra Chip Diseases
Specific Cooperative Agreement (S)
Accession Number: 419395
Development of Ssr Markers for Genetic Diversity Assessment of Candidatus Liberibacter Strains in Florida
Trust (T)
Accession Number: 416602
Publications
Transcriptional Regulation of the Grape Cytochrome P450 Monooxygenase Gene CYP736B Expression in
Response to Xylella fastidiosa Infection - (Peer Reviewed Journal)
Cheng, D.W., Lin, H., Takahashi, Y., Walker, A.M., Civerolo, E.L., Stenger, D.C. 2010. Transcriptional
Regulation of the Grape Cytochrome P450 Monooxygenase Gene CYP736B Expression in Response to
Xylella fastidiosa Infection. Biomed Central (BMC) Plant Biology. 10:135.
Extracellular Xylella fastidiosa genomic DNA enhances biofilm formation in vitro - (Abstract)
Lin, H., Cheng, D.W., Civerolo, E.L. 2010. Extracellular Xylella fastidiosa genomic DNA enhances biofilm
formation in vitro. Phytopathology. 100:S72.
A new molecular diagnostic tool for quantitatively detecting and genotyping "Candidatus Liberibacter
species" - (Abstract)
Lin, H., Liao, H., Bai, Y., Civerolo, E.L. 2010. A new molecular diagnostic tool for quantitatively detecting and
genotyping "Candidatus Liberibacter species". Phytopathology. 100:S72.
Comparative Analyses Of The `Candidatus Liberibacter- Species Reductive Genome Features - (Abstract)
Doddapaneni, H., Lin, H., Duan, Y., Lou, B., Chen, C., Vahling, C.M., Lijuan, Z., Civerolo, E.L. 2010.
Comparative Analyses Of The `Candidatus Liberibacter- Species Reductive Genome Features.
Phytopathology. 100:S30.
SSR-based Genetic Analysis of "Candidatus Liberibacter asiaticus" isolates from Multiple Continents. (Abstract)
Glynn, J.M., Lin, H., Chen, C., Duan, Y., Zhou, L. 2010. SSR-based Genetic Analysis of "Candidatus
Liberibacter asiaticus" isolates from Multiple Continents.. Phytopathology. 100:S41.
A New Diagnostic system for Ultra Sensitive and Specific Detection and Quantitation of "Candidatus
Liberibacter asiaticus", the Bacterium Associated with Citrus Huanglongbing - (Peer Reviewed Journal)
Lin, H., Chen, C., Doddapaneni, H., Duan, Y., Civerolo, E.L., Bai, X., Zhao, X. 2010. A New Diagnostic
system for Ultra Sensitive and Specific Detection and Quantitation of "Candidatus Liberibacter asiaticus", the
Bacterium Associated with Citrus Huanglongbing. Journal of Microbiological Methods. 81(1):17-25.
Extracellular Genomic DNA Mediates Enhancement of Xylella fastidiosa Biofilm Formation in Vitro - (Peer
Reviewed Journal)
Cheng, D.W., Lin, H., Civerolo, E.L. 2010. Extracellular Genomic DNA Mediates Enhancement of Xylella
fastidiosa Biofilm Formation in Vitro. Journal of Plant Pathology. 92(2):405-410.
Xylella fastidiosa Extracellular Genomic DNA May Play a Role For Enhancing Biofilm Formation In Vitro (Proceedings/Symposium)
Lin, H., Cheng, D.W., Civerolo, E.L. 2009. Xylella fastidiosa Extracellular Genomic DNA May Play a Role For
Enhancing Biofilm Formation In Vitro. In: CDFA Pierce's Disease Control Program Research Symposium,
December 9-11, 2009, Sacramento, CA. p. 96-99.
Detection, Distribution and Genetic Variability of 'Candidatus Liberibacter- Species Associated with Zebra
Complex Disease of Potato in North America - (Peer Reviewed Journal)
Wen, A., Mallik, I., Alvarado, V.Y., Pasche, J.S., Wang, X., Li, W., Levy, L., Lin, H., Scholthof, H., Mirkov, E.,
Rush, C.M., Gudmestad, N.C. 2009. Detection, Distribution and Genetic Variability of 'Candidatus
Liberibacter- Species Associated with Zebra Complex Disease of Potato in North America. Plant Disease.
93:1102-1115.
Evaluation of Quantitative Real-Time PCR Assays for Detection of Citrus Greening (Proceedings/Symposium)
Turechek, W., Gottwald, T.R., Hartung, J.S., Hilf, M.E., Keremane, M.L., Lin, H., Shatters, R.G., Irey, M.,
Sieburth, P., Brlansky, R., Dagraca, J., Graham, J., Kunta, M., Roberts, P., Rogers, M., Sun, X., Wang, N.
2009. Evaluation of Quantitative Real-Time PCR Assays for Detection of Citrus Greening. Workshop
Proceedings.
Incidence, Distribution, and Genetic Variations of `Candidatus Liberibacter sp.' Associated with Zebra Chip of
Potato in North America. - (Abstract)
Wen, A., Mallik, I., Pasche, J.S., Wang, X., Li, W., Levy, L., Lin, H., Alvarado, V.Y., Scholthof, H., Mirkov, E.,
Rush, C.M., Gudmestad, N.C. 2009. Incidence, Distribution, and Genetic Variations of `Candidatus
Liberibacter sp.' Associated with Zebra Chip of Potato in North America. Phytopathology(99):S140.
Draft Genome Sequence of Potato `Zebra Chip" Associated Bacterium `Candidatus Liberibacter
solanacearum" - (Abstract)
Lin, H., Doddapaneni, H., Chen, C., Duan, Y., Zhou, L., Civerolo, E.L., Stenger, D.C. 2009. Draft Genome
Sequence of Potato `Zebra Chip" Associated Bacterium `Candidatus Liberibacter solanacearum". American
Phytopathological Society Annual Meeting, Portland, OR (07/09). Phytopathology 99:S73.
Identification of Grapevine Xylem Sap Protein Profiles in Response to Xylella fastidiosa Infection - (Abstract)
Lin, H., Cheng, D.W., Felix, F.B., Walker, A.M. 2009. Identification of Grapevine Xylem Sap Protein Profiles
in Response to Xylella fastidiosa Infection. Phytopathology(99):S74.
Development of SSR Markers for Detection, Genotyping, and Genetic Diversity Assessment of "Candidatus
Liberibacter asiaticus" - (Abstract)
Lin, H., Doddapaneni, H., Chen, C., Duan, Y., Zhou, L. 2009. Development of SSR Markers for Detection,
Genotyping, and Genetic Diversity Assessment of "Candidatus Liberibacter asiaticus".
Phytopathology(99):S74.
Effects of Grape Xylem Sap and Cell Wall Constituents on In Vitro Growth, Biofilm Formation, and Cellular
Aggregation of Xylella fastidiosa - (Peer Reviewed Journal)
Cheng, D.W., Lin, H., Walker,, M., Stenger, D.C., Civerolo, E.L. 2009. Effects of Grape Xylem Sap and Cell
Wall Constituents on In Vitro Growth, Biofilm Formation, and Cellular Aggregation of Xylella fastidiosa.
European Journal of Plant Pathology. 125:213-222.
Sabath Methyltransferases from White Spruce (Picea Glauca [moench] Voss): Gene Cloning, Functional
Characterization and Structural Analysis - (Peer Reviewed Journal)
Zhao, N., Boyle, B., Duval, I., Ferrer, J., Lin, H., Seguin, A., Mackay, J., Chen, F. 2009. SABATH
Methyltransferases from White Spruce (Picea glauca [Moench] Voss): Gene Cloning, Functional
Characterization and Structural Analysis. Tree Physiology. 29(7):947-957.
Complete Genome sequence of citrus huanglongbing bacterium, `Candidatus Liberibacter asiaticusobtained through metagenomics - (Peer Reviewed Journal)
Duan, Y., Zhou, L., Hall, D.G., Li, W., Doddapaneni, H., Lin, H., Liu, L., Gabriel, D., Vahling, C.M., Williams,
K., Dickerman, A., Sun, Y., Gottwald, T.R. 2009. Complete Genome sequence of citrus huanglongbing
bacterium, `Candidatus Liberibacter asiaticus- obtained through metagenomics. Molecular Plant-Microbe
Interactions. 22:1011-1020.
Effects of Grape Xylem Sap and Cell-Wall Constituents on In Vitro Growth, Biofilm Formation and Cellular
Aggregation of Xylella fastidiosa - (Proceedings/Symposium)
Cheng, D.W., Lin, H., Walker, A., Stenger, D.C., Civerolo, E.L. 2008. Effects of Grape Xylem Sap and
Cell-Wall Constituents on In Vitro Growth, Biofilm Formation and Cellular Aggregation of Xylella fastidiosa.
Proceedings of the CDFA Pierce's Disease Control Program Research Symposium, December 15-17, 2008,
San Diego, California. pp.118-122.
Selective Usage of Transcription Initiation and Polyadenylation Sites in Grape Cytochrome P450
Monooxygenase Gene CYP736B Expression - (Abstract)
Cheng, D.W., Lin, H., Stenger, D.C., Walker, A.M., Civerolo, E.L. 2008. Selective Usage of Transcription
Initiation and Polyadenylation Sites in Grape Cytochrome P450 Monooxygenase Gene CYP736B Expression.
Meeting Abstract. Pg. 163.
Breeding Pierce's disease resistant table and raisin grapes and the development of markers for additional
sources of resistance 2008 - (Proceedings/Symposium)
Ramming, D.W., Walker, A., Lin, H. 2008. Breeding Pierce's disease resistant table and raisin grapes and
the development of markers for additional sources of resistance 2008. CDFA Pierce's Disease Control
Program Research Symposium. Pg. 235-238.
A novel molecular diagnostic tool for improved sensitivity and reliability detection of "Candidatus Liberibacter
asiaticus", bacterium associated with huanglongbing (HLB) bacterium Candidatus Liberibacter. - (Abstract)
Lin, H., Doddapaneni, H., Civerolo, E.L. 2008. A novel molecular diagnostic tool for improved sensitivity and
reliability detection of "Candidatus Liberibacter asiaticus", bacterium associated with huanglongbing (HLB)
bacterium Candidatus Liberibacter. International Research Conference on Huanglongbing:Pg. 117-118,
Orlando, Florida, December 1-5, 2008.
Molecular characterization and Phylogenetic analysis of 16S rRNA from a new Candidatus Liberibacter strain
associated with Zebra chip disease of Potato (Solanum tuberosum L.) and the potato psyllid - (Peer
Reviewed Journal)
Lin, H., Doddapaneni,, H., Munyaneza, J.E., Civerolo, E.L., Venkatesan, S.G., Buchman, J.L., Stenger, D.C.
2009. Molecular characterization and Phylogenetic analysis of 16S rRNA from a new Candidatus
Liberibacter strain associated with Zebra chip disease of Potato (Solanum tuberosum L.) and the potato
psyllid. Journal of Plant Pathology. 91:215-219.
PrimerSNP: a web tool for whole-genome selection of allele-specific and common primers of
phylogenetically-related bacterial genomic sequences - (Peer Reviewed Journal)
Yao, J., Lin, H., Vandeyne, A., Doddapaneni, H., Francis, M., Macedo Lemos, E., Civerolo, E.L. 2008.
PrimerSNP: a web tool for whole-genome selection of allele-specific and common primers of
phylogenetically-related bacterial genomic sequences. BMC Microbiology,
8:185doi:10.1186/1471-2180-8-185.
Comparative phylogenomics and multi-gene cluster analyses of the CitrusHuanglongbing (HLB)-associated
bacterium Candidatus Liberibacter - (Peer Reviewed Journal)
Doddapaneni, H., Liao,, H., Lin, H., Bai,, X., Zhao,, X., Civerolo, E.L., Irey,, M., Coletta-Filho,, H., Pietersen,,
G. 2008. Comparative phylogenomics and multi-gene cluster analyses of the CitrusHuanglongbing
(HLB)-associated bacterium Candidatus Liberibacter. BMC Research Notes, 1:72.
Molecular Characterization of the Citrus Huanglongbing (HLB) associated bacterium Candidatus Liberibacter
- (Abstract)
Doddapaneni, H., Lin, H., Liao, H., Bai, X., Zhao, X., Civerolo, E.L. 2008. Molecular Characterization of the
Citrus Huanglongbing (HLB) associated bacterium Candidatus Liberibacter. International Citrus Congress
Proceedings: Pg. 53.
Characterization and Identification of Pd Resistance Mechanisms: Analyses of Xylem Anatomical Structures
and Molecular Interactions of Host/xylella Fastidiosa - (Abstract)
Lin, H., Doddapaneni,, H., Fritschi,, F.B., Walker,, M. 2008. Characterization and identification of pd
resistance mechanisms: analyses of xylem anatomical structures and molecular interactions of host/xylella
fastidiosa. In: Proceedings of 2nd Annual Viticulture Research Conference, July 9-11,2008, Davis, California.
p.46-47.
Environmental factors affecting twitching motility, biofilm development, and aggregation by Xylella fastidiosa (Abstract)
Delafuente, L., Zaini,, P., Cursino,, L., Lin, H., Burr,, T.J., Hoch,, H.C. 2008. Environmental factors affecting
twitching motility, biofilm development, and aggregation by Xylella fastidiosa. American Phytopathological
Society Annual Meeting.
Characterization of Growth and Virulence-related Genes Expression of Xylella fastidiosa Affected by Grape
Xylem Sap and Cell-Wall Constituents - (Abstract)
Cheng, D.W., Lin, H., Walker, M., Stenger, D.C., Civerolo, E.L. 2008. Characterization of Growth and
Virulence-related Genes Expression of Xylella fastidiosa Affected by Grape Xylem Sap and Cell-Wall
Constituents. Phytopathology 98:S37.
Transcriptional Regulation of Grape Cytochrome P450 Gene Expression in Response to Xylella fastidiosa (Abstract)
Cheng, D.W., Lin, H., Walker,, M., Stenger, D.C., Civerolo, E.L. 2008. Transcriptional Regulation of Grape
Cytochrome P450 Gene Expression in Response to Xylella fastidiosa. Phytopathology 98:S37.
Comparative Phylogenomics and Multi-gene cluster analyses of the Citrus Huanglongbing (HLB)-associated
bacterium Candidatus Liberibacter - (Abstract)
Doddapaneni, H., Liao,, H., Lin, H., Bai,, X., Zhao,, X., Civerolo, E.L. 2008. Comparative Phylogenomics and
Multi-gene cluster analyses of the Citrus Huanglongbing (HLB)-associated bacterium Candidatus
Liberibacter. American Phytopathological Society Annual Meeting. 98:S47.
Evaluation of the Genetic Structure of Xylella fastidiosa Populations Collected from Almond orchards in
California - (Abstract)
Thimmiraju,, S.R., Daane,, K., Groves,, R.L., Lin, H., Sisterson, M.S. 2008. Evaluation of the Genetic
Structure of Xylella fastidiosa Populations Collected from Almond orchards in California. Phytopathology
98:S156-S156.
VitisExpDB: A Database Resource for Grape Functional Genomics - (Peer Reviewed Journal)
Doddapaneni,, H., Lin, H., Walker, M., Yao, J., Civerolo, E.L. 2008. vitisExpDB: A database resource for
grape functional genomics. Biomed Central (BMC) Plant Biology.8:23doi:10.1186/1471-2229-8-23
Breeding Pierce's Disease Resistant Table and Raisin Grapes and the Development of Markers for
Additional Sources of Resistance - (Proceedings/Symposium)
Ramming, D.W., Walker,, A.M., Lin, H. 2007. Breeding pierce's disease resistant table and raisin grapes and
the development of markers for additional sources of resistance. In: CDFA Pierce's Disease Control Program
Research Symposium. p. 271-273.
Microarray Analysis of Global Gene Expression of V. vinifera in Response to Xylella fastidiosa Infection (Proceedings/Symposium)
Lin, H., Harshavardhan,, D., Walker,, A.M. 2007. Microarray Analysis of Global Gene Expression of V.
vinifera in Response to Xylella fastidiosa Infection. In: Proceedings of CDFA Pierce's Disease Control
Program Research Symposium, December 12-14, 2007, San Diego, California. p. 254-257.
Hierarchical Analysis and Diversity Studies of Xylella fastidiosa Populations in California by Multi-locus
Simple Sequence Repeat Markers - (Proceedings/Symposium)
Lin, H., Thammiraju,, S., Walker,, A.M., Stenger, D.C., Civerolo, E.L. 2007. Hierarchical Analysis and
Diversity Studies of Xylella fastidiosa Populations in California by Multi-locus Simple Sequence Repeat
Markers. In: Proceedings of CDFA Pierce's Disease Control Program Research Symposium, December
12-14, 2007, San Diego, California. p. 144-147.
In vitro Study of the Effects of Grape Xylem Saps and Cell-Wall Constituents on Growth and
Virulence-Related Gene Expression of Xylella fastidiosa - (Abstract)
Cheng, D.W., Lin, H., Walker,, A.M., Stenger, D.C., Civerolo, E.L. 2007. In vitro Study of the Effects of Grape
Xylem Saps and Cell-Wall Constituents on Growth and Virulence-Related Gene Expression of Xylella
fastidiosa [abstract]. Proceedings of CDFA Pierce's Disease Control Program Research Symposium. p. 291.
Acquisition of Uncharacterized Sequences from Candidatus Liberibacter, an unculturable bacteria, using an
improved genomic walking method - (Peer Reviewed Journal)
Lin, H., Doddapaneni, H., Bai, X., Yao, J., Zhao, X., Civerolo, E.L. 2008. Acquisition of Uncharacterized
Sequences from Candidatus Liberibacter, an unculturable bacteria, using an improved genomic walking
methodAcquisition of Uncharacterized Sequences from Candidatus Liberibacter, an unculturable bacteria,
using an improved genomic walking method. 22:30-37.
Molecular Cloning and Biochemical Characterization of Indole-3-acetic Acid Methyltransferase from Poplar
(Populus trichocarpa) - (Peer Reviewed Journal)
Zhao, N., Guan, J., Lin, H., Chen, F. 2007. Molecular Cloning and Biochemical Characterization of
Indole-3-acetic Acid Methyltransferase from Poplar (Populus trichocarpa). Phytochemistry. 68:1537-1544.
Behavioral Responses of Homalodisca coagulata (Hemiptera: Auchenorrhyncha: Cicadellidae) on Four Vitis
Genotypes - (Peer Reviewed Journal)
Fritschi, F., Cabrera-La Rosa, J.C., Groves, R.L., Lin, H., Johnson, M.W. 2007. Behavioral Responses of
Homalodisca vitripennis (Hemiptera: Auchenorrhyncha: Cicadellidae) on Four Vitis Genotypes.
Environmental Entomology. Vol. 36, Issue 4, pp. 926-936.
Comparative Genomics and Phylogenetic Analyses of Newly Cloned Genomic Regions From the Citrus
Huanglongbing (HLB)-associated Bacterium Candidatus Liberibacter - (Abstract)
Doddapaneni, H., Lin, H., Bai, X., Zhao, X., Civerolo, E.L. 2007. Comparative Genomics and Phylogenetic
Analyses of Newly Cloned Genomic Regions From the Citrus Huanglongbing (HLB)-associated Bacterium
Candidatus Liberibacter. American Phytopathology Society. 97(7):29
Genome-wide Profiling and Analysis of the Vitis Transcriptome Responses to Xylella fastidiosa Infection (Abstract)
Doddapaneni, H., Lin, H., Takahashi, Y., Walker, A.M. 2007. Genome-Wide Profiling and Analysis of the Vitis
Transcriptome Responses to Xylella fastidiosa Infection. American Society of Plant Biologists Annual
Meeting. p. 89.
Novel Molecular Technique for Rapid Cloning of Unknown Sequences From Unculturable Huanglongbing
(HLB) Associated Bacterium, Candidatus Liberibacter - (Abstract)
Lin, H., Doddapaneni, H., Liao, H., Bai, X., Zhao, X., Wang, J., Civerolo, E.L. 2007. Novel Molecular
Technique for Rapid Cloning of Unknown Sequences From Unculturable Huanglongbing (HLB) Associated
Bacterium, Candidatus Liberibacter. American Phytopathology Society. 97(7):64
Utilization of Genomic Variations Among Xylella fastidiosa Strains for Improved Diagnostic Design (Abstract)
Lin, H., Doddapaneni, H., Yao, J., Civerolo, E.L. 2007. Utilization of Genomic Variations Among Xylella
fastidiosa Strains for Improved Diagnostic Design. American Phytopathology Society. 97(7):64
Identification of Proteomic Expression of Grapevines in Response to Xylella fastidiosa - (Abstract)
Yang, L., Lin, H., Takahashi, Y., Walker, A.M. 2007. Identification of Proteomic Expression of Grapevines in
Response to Xylella fastidiosa. American Phytopathology Society. 97(7):127
nWayComp: A Tool for Universal Comparison of DNA and Protein Sequences - (Peer Reviewed Journal)
Yao, J., Lin, H., Doddapaneni, H., Civerolo, E.L. 2007. nWayComp: A Tool for Universal Comparison of DNA
and Protein Sequences. In Silico Biology. 7(2):195-200
Xylella Fastidiosa Population Dynamics in Grapevine Genotypes Differing in Susceptibility to Pierce's
Disease - (Peer Reviewed Journal)
Fritschi, F.B., Lin, H., Walker, A. 2007. Xylella fastidiosa Population Dynamics in Grapevine Genotypes
Differing in Susceptibility to Pierce's Disease. American Journal of Enology and Viticulture. 58(3):326-332.
Comparative Analysis of Est's Involved in Grape Responses to Xylella Fastidiosa Infection - (Peer Reviewed
Journal)
Lin, H., Doddapaneni, H., Takahashi, Y., Walker, A. 2007. Comparative analysis of est's involved in grape
responses to Xylella fastidiosa infection. Biomed Central (BMC) Plant Biology 2007, 7:8,
doi:10.1186/1471-2229-7-8
Genome-Wide Analysis of Xylella Fastidiosa: Implications for Detection and Strain Relationships - (Peer
Reviewed Journal)
Doddapaneni, H., Francis, M., Yao, J., Lin, H., Civerolo, E.L. 2007. Genome-wide Analysis of Xylella
fastidiosa: Implications for Detection and Strain Relationships. African Journal of Biotechnology. 6(2):55-66.
Epidemiology of Almond Leaf Scorch Disease in the San Joaquin Valley of California: Factors Affecting
Pathogen Distribution and Movement - (Proceedings/Symposium)
Groves, R., Sisterson, M.S., Chen, J., Lin, H. 2007. Epidemiology of Almond Leaf Scorch Disease in the San
Joaquin Valley of California: factors affecting pathogen distribution and movement. In: Proceedings of the
34th Almond Industry Conference, December 6-7, 2006, Modesto, California. p. 176-183.
Microarray Gene Expression Analysis of Grape Plants in Response to Xylella Fastidiosa Infection (Proceedings/Symposium)
Lin, H., Doddapaneni, H., Yao, J., Walker, A. 2006. Microarray gene expression analysis of grape plants in
response to xylella fastidiosa infection, Pierce's Disease Control Program Research Symposium, San Diego,
CA., November 27-29, 2006, p. 290-293.
Comparative Proteomic Analysis of Stem Tissue and Xylem Sap from Pierce's Disease Resistant and
Susceptible Grapevines - (Proceedings/Symposium)
Lin, H., Fritschi, F.B., Yang, L., Walker, A. 2006. Comparative proteomic analysis of stem tissue and xylem
sap from pierce's disease resistant and susceptible grapevines. Proceedings of CDFA Pierce's Disease
Control Program Research Symposium, November 27-29, 2006. p.294-297.
Multilocus Simple Sequence Repeats and Single Nucleotide Polymorphism Markers for Genotyping and
Assessing Genetic Diversity of Xylella Fastidiosa in California - (Proceedings/Symposium)
Lin, H., Civerolo, E.L., Doddapaneni, H., Groves, R., Walker, A. 2006. Multilocus simple sequence repeats
and single nucleotide polymorphism markers for genotyping and assessing genetic diversity fo xylella
fastidiosa in california. Proceedings of CDFA Pierce's Disease Control Program Research Symposium,
November 27-29, 2006, San Diego, California. p.160-163.
Breeding Pierce's Disease Resistant Table and Raisin Grapes and the Development of Markers for
Additional Sources of Resistance - (Proceedings/Symposium)
Ramming, D.W., Walker, A.M., Lin, H. 2006. Breeding Pierce's Disease Resistant Table and Raisin Grapes
and the Development of Markers for Additional Sources of Resistance. 2006 CDFA Pierce's Disease Control
Program Research Symposium. p. 298-300.
New Standard Protocol for Diagnosis of Xylella Fastidiosa - (Abstract)
Francis, M., Civerolo, E.L., Doddapaneni, H., Lin, H., Bruening, G. 2006. New standard protocol for diagnosis
of xylella fastidiosa [abstract]. CDFA Pierce's Disease Control Program Research Symposium. p.203.
Analysis of the Genome-Wide Variations among Multiple Strains of the Plant Pathogenic Bacterium Xylella
Fastidiosa - (Peer Reviewed Journal)
Doddapaneni, H., Yao, J., Lin, H., Walker, A., Civerolo, E.L. 2006. Analysis of the genome-wide variations
among multiple strains of the plant pathogenic bacterium xylella fastidiosa. Biomed Central (BMC) Genomics.
Available: http://www.biomedcentral.com/1471-2164/7/225
The Endo-Beta-Mannanase Gene Families in Arabidopsis, Rice and Poplar - (Peer Reviewed Journal)
Yuan, J.S., Koizuka, N., Yang, X., Lai, I., Lin, H., Cheng, Z., Nonogaki, H., Chen, F. 2006. The
Endo-beta-mannanase Gene Families in Arabidopsis, Rice and Poplar. Functional and Integrative Genomics.
7:1-16.
Genome-Based Pcr Primers for Specific and Sensitive Detection and Quantification of Xylella Fastidiosa (Peer Reviewed Journal)
Francis, M., Lin, H., Cabrera, J.C., Doddapaneni, H., Civerolo, E.L. 2006. Genome-based pcr primers for
specific and sensitive detection and quantification of xylella fastidiosa. European Journal of Plant Pathology.
115(2):203-213.
Multiplex Taq-Man Pcr for Sensitive and Accurate Quantification Bio-Threat Crop Pathogens; Xylella
Fastidiosa and Candidatus, Liberibacter - (Abstract)
Lin, H., Doddapaneni, H., Yao, J., Civerolo, E.L. 2006. Multiplex TAQ-MAN PCR for Sensitive and Accurate
Quantification Bio-threat Crop Pathogens; Xylella fastidiosa and candidatus, liberibacter. Phytopathology.
96:S127.
Multiplex Taqman-Based Pcr for Sensitive and Accurate Quantification of Citrus Hlb and Cvc Pathogens;
Candidatus Liberibacter and Xylella Fastidiosa - (Abstract)
Lin, H., Doddapaneni, H., Civerolo, E.L., Yao, J., Yokomi, R.K., Bai, X., Cao, H., Zhao, X., Wen, R. 2006.
Multiplex taqman-based pcr for sensitive and accurate quantification of citrus hlb and cvc pathogens;
candidatus liberibacter and xylella fastidiosa [Abstract]. Proceedings of the Huanglongbing-greening
International Workshop. p. 83.
High-Throughput Microarray Comparative Genomic Analyses of Xylella Fastidiosa Strains - (Abstract)
Yao, J., Doddapaneni, H., Lin, H., Civerolo, E.L. 2006. High-throughput microarray comparative genomic
analyses of xylella fastidiosa strains. [abstract] Phytopathology. 96:S127.
Microarray Gene Expression Analysis to Better Understand the Grape / Xylella Fastidiosa Interaction. (Abstract)
Lin, H., Doddapaneni, H., Walker, A. 2006. Microarray gene expression analysis to better understand the
grape / xylella fastidiosa interaction. [abstract}. 9th International Conference on Grape Genetics and
Breeding. p.184
Proteomic Analysis of Expression Profiles of Pierce's Disease Resistant and Susceptible Grapevines in
Response to Xylella Fastidiosa - (Abstract)
Lin, H., Fritschi, F.B., Yang, L., Walker, A. 2006. Proteomic analysis of expression profiles of pierce's disease
resistant and susceptible grapevines in response to xylella fastidiosa [Abstract]. 9th International Conference
on Grape Genetics and Breeding. p.97
Gene Expression Profiling of the Grape Xylella Fastidiosa Interaction - (Abstract)
Lin, H., Doddapaneni, H., Yao, J., Walker, A. 2006. Gene expression profiling of the grape xylella fastidiosa
interaction. American Society of Plant Biologists Annual Meeting, 2006. p. 174.
Identification and Characterization of Proteomic Expression of Grapevines in Response to Xylella Fastidiosa
- (Abstract)
Yang, L., Lin, H., Fritschi, F.B., Walker, A. 2006. Identification and characterization of proteomic expression
of grapevines in response to xylella fastidiosa. American Society of Plant Biologists Annual Meeting 2006. p.
174.
Xylem Sap and Xylem Anatomical Aspects of Various Vitis Genotypes in Respect to Pierce's Disease (Abstract)
Lin, H., Fritschi, F.B., Walker, A. 2006. Xylem sap and xylem anatomical aspects of various vitis genotypes in
respect to Pierce's disease [Abstract]. AgBiotech 2006 Conference, The Central California Agricultural
Biotechnology Symposium. p. 29.
Global Expression Profiling of the Grape / Xylella Fastidiosa Interactions - (Abstract)
Lin, H., Doddapaneni, H., Walker, A., Yao, J. 2006. Global expression profiling of the grape / xylella
fastidiosa interactions [Abstract]. AgBiotech 2006 Conference, The Central California Agricultural
Biotechnology Symposium. p. 26.
Profiling Proteomic Expression of Grapes in Response to Pierce's Disease - (Abstract)
Yang, L., Lin, H., Fritschi, F.B., Walker, A. 2006. Profiling proteomic expression of grapes in response to
Pierce's disease [Abstract] AgBiotech 2006 Conference, The Central California Agricultural Biotechnology
Symposium. p.39.
New Simple Sequence Repeat Loci for the Study of Grape Phylloxera (Daktulosphaira Vitifoliae) Genetics
and Host Adapation. - (Peer Reviewed Journal)
Lin, H., Walker, A., Granett, J. 2006. New Simple Sequence Repeat Loci for the Study of Grape Phylloxera
(Daktulosphaira vitifoliae) Genetics and Host Adapation. American Journal of Enology and Viticulture.
75(1):33-40.
Multi-Locus Ssr Markers for Genotyping and Assessing Genetic Diversity of Xylella Fastidiosa in California (Proceedings/Symposium)
Lin, H., Civerolo, E.L., Groves, R.L., Walker, A. 2005. Multi-locus Simple Sequence Repeat (SSR) Markers
for Genotyping and Assessing Genetic Diversity of Xylella fastidiosa in California. Proceedings for the 2005
Pierce's Disease Research Symposium. p. 170-172.
Comparative Proteomic Analysis of Stem Tissue and Xylem Sap from Pierce's Disease Resistant and
Susceptible Grapevines - (Proceedings/Symposium)
Lin, H., Fritschi, F.B., Yang, L., Walker, A. 2005. Comparative Proteomic Analysis of Stem Tissue and Xylem
Sap from Pierce's Disease Resistant and Susceptible Grapevines. Proceedings of the 2005 Pierce's Disease
Research Symposium. p. 47-49.
Developing Transcriptional Profiles and Gene Expression Analysis of Grape Plant Response to Xylella
Fastidiosa - (Proceedings/Symposium)
Lin, H., Doddapaneni, H., Walker, A. 2005. Developing Transcriptional Profiles and Gene Expression
Analysis of Grape Plant Response to Xylella fastidiosa. Proceedings of the 2005 Pierce's Disease Research
Symposium. p.43-46.
Characterization and Identification of Pd Resistance Mechanisms: Analysis of Xylem Anatomical Structures
and of Natural Products in Xylem Sap among Vitis - (Proceedings/Symposium)
Lin, H., Fritschi, F.B., Walker, A. 2005. Characterization and Identification of Pierce's Disease Resistance
Mechanisms: Analysis of Xylem Anatomical Structures and of Natural Products in Xylem Sap among Vitis.
Proceedings of the 2005 Pierce's Disease Research Symposium. p. 39-42.
The Study of Natural Product Biosynthesis in the Pregenomics and Genomics Eras. - (Book/Chapter)
Chen, F., Cseke, L., Lin, H., Kirakosyan, A., Yuan, J.S., Kaufman, P. 2006. The Study of Natural Product
Biosynthesis in the Pregenomics and Genomics Eras. In: Cseke, L.J., Kirakosyan, A., Kaufman, P.B.,
Warber, S., Duke, J.A., Brielmann, H.L., editors. Natural Products from Plants. Second Edition. Florence, KY:
CRC Press. p. 203-220.
Genome-Wide Comparative Analysis of Differentially Regulated Grape Transcriptome in Response to Xylella
Fastidiosa Infection - (Proceedings/Symposium) - (12-Jun-05)
Seasonal Population Biology of Xylella Fastidiosa Genotypes in Almond and Movement by Insect Vectors (Abstract)
Cabrera, J., Groves, R.L., Chen, J., Lin, H., Francis, M., Civerolo, E.L. 2005. Seasonal population biology of
Xylella fastidiosa genotypes in almond and movement by insect vectors. Phytopatholoy. 95(6):S16.
Genome-Wide Comparative Analysis of Differentially Regulated Grape Transcriptome in Response to Xylella
Fastidiosa Infection - (Abstract)
Lin, H., Takahashi, Y., Doddapaneni, H., Walker, A. 2005. Genome-wide comparative analysis of
differentially regulated grape transcriptome in response to xylella fastidiosa infection. [Abstract]. International
Grape Genomics Symposium. p.43
A High-Through-Put Multi-Locus Pathogen Detecting System for Strain Identification and Population Genetic
Studies of Xylella Fastidiosa - (Abstract)
Lin, H., Civerolo, E.L., Groves, R.L., Takahashi, Y., Walker, A. 2005. A high-through-put multi-locus
pathogen detecting system for strain identification and population genetic studies of Xylella fastidiosa.
Phytopathology. 95(6):S61.
Characterization of Xylem Anatomical Structures and the Effect of Xylem Sap from Vitis to Pierce's Disease (Abstract)
Lin, H., Fritschi, F.B., Walker, A., Barros, S. 2005. Characterization of xylem anatomical structures and the
effect of xylem sap from vitis to pierce's disease. [Abstract]. American Society of Enology and Viticulture
Annual Meeting. p.48
Comparative Analysis of Differentially Regulated Expression of Grapes in Response to Xylella Fastidiosa
Infection - (Abstract)
Lin, H., Walker, A.M., Takahashi, Y., Doddapaneni, H. 2005. Comparative analysis of differentially regulated
expression of grapes in response to xylella fastidiosa infection. [Abstract]. American Society of Plant
Biologists Annual Meeting. p.165.
Multi-Locus Simple Sequence Repeat (Ssr) Markers for Differentiating Strains and Evaluating Genetic
Diversity of Xylella Fastidiosa - (Peer Reviewed Journal)
Lin, H., Civerolo, E.L., Walker, A., Hu, R., Barros, S., Francis, M. 2005. Multi-locus simple sequence repeat
(ssr) markers for differentiating strains and evaluating genetic diversity of xylella fastidiosa. Applied and
Environmental Microbiology. 71:4888-4892.
Characterization and Identification of Pierce's Disease Resistance Mechanisms: Analysis of Xylem
Anatomical Structures and of Natural Products in Xylem Sap among Vitis - (Proceedings/Symposium)
Lin, H., Walker, A. 2004. Characterization and identification of Pierce's Disease resistance mechanisms:
Analysis of xylem anatomical structures and of natural products in xylem sap among Vitis. Proceeding of
CDFA Pierce's Disease Control Program Research Symposium. p. 22-24
Developing Transcriptional Profiles and Gene Expression Analysis of Grape Plant Response to Xylella
Fastidiosa - (Proceedings/Symposium)
Lin, H., Walker, A. 2004. Developing transcriptional profiles and gene expression analysis of grape plant
response to Xylella fastidiosa. Proceedings of CDFA Pierce's Disease Control Program Research
Symposium. p. 25-27.
Development of Ssr Markers for Genotyping and Assessing the Genetic Diversity of Xylella Fastidiosa in
California - (Proceedings/Symposium)
Lin, H., Civerolo, E.L., Walker, A. 2004. Development of SSR markers for genotyping and assessing the
genetic diversity of Xylella fastidiosa in California. Proceedings of CDFA Pierce's Disease Control Program
Research Symposium. p. 206-209.
Profiling Differential Gene Expression of Grape Plants in Response to the Infection of Xylella Fastidiosa
Using Cdna-Aflp - (Abstract)
Lin, H., Takahashi, Y., Hu, R., Walker, A. 2004. Profiling differential gene expression of grape plants in
response to the infection of Xylella fastidiosa using cDNA-AFLP [Abstract]. American Society of Plant Biology.
p. 138.
Genotyping and Assessing Genetic Diversity in Pd Strain Xylella Fastidiosa by Simple Sequence Repeat
(Ssr) DNA Markers - (Abstract)
Lin, H., Civerolo, E.L., Barros, S., Hu, R., Francis, M., Walker, A. 2004. Genotyping and assessing genetic
diversity in PD strain Xylella fastidiosa by simple sequence repeat (SSR) DNA markers [Abstract].
Phytopathology. 94:S61.
Pcr Based Systems for the Detection, Differentiation and Quantification of Xylella Fastidiosa Strains (Abstract)
Francis-Mastalli, F., Civerolo, E.L., Lin, H. 2004. PCR based systems for the detection, differentiation and
quantification of Xylella fastidiosa strains. Phytopathology. 94(6):S31.
Development of Ssr Markers for Genotyping and Assessing Genetic Diversity of Xylella Fastidiosa (Abstract)
Lin, H., Civerolo, E.L., Barros, S., Walker, A. 2004. Development of ssr markers for genotyping and
assessing genetic diversity of Xylella fastidiosa [Abstract]. American Society of Enology and Viticulture. p.
162.
Developing Transcriptional Profiles and Microarray Expression Analysis of Grape Plant Responses to Xylella
Fastidiosa - (Abstract)
Lin, H., Walker, A. 2003. Developing transcriptional profiles and microarray expression analysis of grape
plant responses to Xylella fastidiosa [Abstract]. American Society of Plant Biology. p. 162.
Developing Gene Expression Profiles for Research on Grape Responses to Xylella Fastidiosa - (Abstract)
Lin, H., Walker, A. 2003. Developing gene expression profiles for research on grape responses to Xylella
fastidiosa [Abstract]. American Society of Enology and Viticulture. p. 51.
Mission:
The mission of the Crop Diseases, Pests and Genetics Research Unit is to (1)develop unique and improved
methods to minimize production losses in horticultural crops in California caused by citrus tristeza
closterovirus (CTV) and introduced plant pathogens and insect pests, primarily Xylella fastidiosa (Xf) and
Homalodisca coagulata, the glassy-winged sharpshooter (GWSS); and (2) develop improved almond, stone
fruit and grape cultivars for fresh and processing markets. Current research on Xf and the GWSS is focused
on Pierce's disease of grapevines and almond leaf scorch disease. The research is conducted under
National Programs 301 (Plant, Microbial and Insect Genetics), 303 (Plant Diseases) and 304 (Crop
Protection and Quarantine). The overall goals are to develop economically, socially and environmentally
acceptable integrated disease and insect pest management strategies based on improved understanding of
disease epidemiology, pathogen biology and ecology, insect vector biology and ecology, complex host
plant-pathogen-vector interactions, and host plant resistance; and improve perennial fruit crop varieties,
quality and disease resistance through breeding and marker-assisted selection. Research approaches
include development of induced and genetic host resistance to diseases and pests; characterization of
pathogen populations; determination of the biotic and abiotic factors that affect insect vector behavior;
elucidation of the mechanisms by which insect vectors acquire and transmit Xf and CTV; and determination
of the physiological and biochemical responses of host plants, including the genetic and molecular bases of
these responses. Predictive models will be developed to describe, forecast and assess the risk of potential
invasive plant pest introduction, spread and establishment.