THE UNIVERSITY OF TEXAS M. D. ANDERSON CANCER CENTER Research Skills Survey Applicant Name: Date: Position: In order for our research recruiters to thoroughly evaluate your skills for research positions, please complete this skill survey by reviewing each category, and indicating your proficiency using the numerical ranking on a scale of 1-10. One being the most expert, and Ten being no experience. Blank rows will indicate no interest. (This will not substitute for a resume or application) FIELD OF WORK ExperienceRanking Bacteriology 3 Biochemistry 1 Other (specify) FIELD OF WORK Experience Ranking Immunology/Cellular Laser Biology Immunology Developmental Biology Antibody production Biochemistry Antibody purification Cell Biology Immunofluorescence Chemistry Flow Cytometry/cell sorting Molecular Epidemiology Immunodiffusion Genetics ELISA Immunology Western blotting Microbiology Lymphocyte function tests Molecular Biology Cytotoxicity assays Neurobiology Proliferation assays Pathology Cytokine assays Pharmacology Mixed lymphocyte culture Physics Monoclonal antibodies Physiology Macrophage culture Tumor Biology NK cell methods Veterinary Medicine T Cell methods Virology Other (specify) Experience Ranking Radiation Biology MOLECULAR BIOLOGY Toxicology METHODS Experience Ranking DNA / RNA Isolation Tumor suppressor genes Sequencing-nucleic acids FISH Sequencing-protein Other (specify) PCR RADIOISOTOPE METHODS Experience Ranking Plasmid preparation CDNA synthesis Radiotracers Gene cloning-plasmid Radiolabeling -phage Liquid scintillation counting -cosmid Gamma counting Library screening Irradiation techniques Restriction mapping Radioimmuno assays Hybridization Radioligand binding assays -In situ Isotopes of exp., please specify -Southern (i.e. P-32; H-3; C-14, etc.) -Northern Other (specify) Transfection Retroviruses Retroviral infection Oncogenes Recombinant DNA Mammalian expression systems RNA purification ANALYTICAL/ QUANTITATIVE METHODS Chromatography - thin layer - column - HPLC - FPLC - GLC Spectrometry - UV / VIS - IR - NMR Ultracentrifugation Organelle purification Electrophoresis Expereince Ranking - gel - PAGE - 2-D - pulsed field Electrofocussing Chemical analysis Physical-Biochemical methodolog Enzyme assays Protein assays DNA / RNA assays Growth factor assays Enzyme / protein purification Lipid biochemistry Adenyl cyclase assays Amino acid assays Other (specify) VETERINARY METHODS Light Phase Fluorescence Electron Microscopy - scanning - transmission Confocal imccroscopy Tissue preparation - EM - frozen section Cytogenetics Immunohistochemistry Histochemistry Micromanipulation Other (specify) GENERAL LABORATORY SKILLS PH measurement Sterile technique Solution preparation Record keeping Low speed centrifugation Photography / Darkroom Statistical Analysis Other (specify) Experience Ranking Animal care technology Surgery Microsurgery Necropsy Ascites production Tumor passage Transgenic animals SCID mice Gene knockout technology Homologous recombination In vivo immunological assays In vitro Metastasis assays Other (specify) COMPUTER SKILLS Macintosh Mainframe Micros PC IBM Operating Systems Programming languages (specify) Image analysis Other (specify) GENERAL SKILLS QUALIFICATION Supervising / Managing experience: ________ Oncology related work exp.: Yes HISTOLOGY No Experience Ranking CELL TISSUE CULTURE Primary cultures Established cultures Fresh human tumor cell Suspension preps Cell transformation assays Mutagenesis assays Media preparation Mycoplasma testing Virus propagation Microinjection techniques Hybridomes Other (specify) MICROSCOPY Experience Ranking Experience Ranking # employees Priority Ranking