Primer naming rules
F
R
Fp
Rp
forward primer
reverse primer
forward primer, phosphorylated
reverse primer, phosphorylated
F-Trim56
R-Trim56
R-Trim56x
forward primer for Trim56 ORF, with start codon (same as F-Trim56-1)
reverse primer for Trim56 ORF, with stop codon (same as R-Trim56-755)
reverse primer for Trim56 ORF, no stop codon (same as R-Trim56-755x)
F-Trim56-m121
forward primer for Trim56, 5' end is ATG, followed by the codon for residue 121
forward primer for Trim56, 5' end is the codon for residue 121, no start codon
Note: Use this name even if residue 121 is a methionine
reverse primer for Trim56, 3' end of complementary strand is the codon for residue 204, with stop codon
reverse primer for Trim56, 3' end of complementary strand is the codon for residue 204, no stop codon
F-Trim56-121
R-Trim56-204
R-Trim56-204x
Old primer names:
F-Trim56-121-NdeI
R-Trim56-204-BamHI
R-Trim56-204x-BamHI
forward primer for Trim56 containing NdeI site
Note: Primer has overhangs and is used to amplify fragments for RE cloning; NdeI, NcoI, PciI sites contain ATG
forward primer for Trim56 containing BamHI site, typically has a stop codon
Note: Primer has overhangs and is used to amplify fragments for RE cloning
forward primer for Trim56 containing BamHI site, with no stop codon
Note: Primer has overhangs and is used to amplify fragments for RE cloning, may contain additional sequences
Quikchange primers - Note that these primers are always ordered in pairs
qcF-Trim56-R310S
forward primer to mutagenize residue 310 from Arg to Ser
qcR-Trim56-R310S
reverse primer to mutagenize residue 310 from Arg to Ser