Supporting Information, Figures S1-S5 S1. Known Iberian records (10x10 UTM cells) of Ochthebius glaber and O. notabilis. Data from Sánchez-Fernández et al. (2008). S2. Known Iberian records (10x10 UTM cells) of Nebrioporus baeticus and N. ceresyi. Data from Sánchez-Fernández et al. (2008). Two records of N. ceresyi from Galicia and Extremadura have been omitted, as they likely represent isolated specimens and not established populations (see text). Figure S3. Plots of the values of fixation indices obtained from SAMOVA as a function of the number of groups (K). FCT: differentiation between groups, FSC: differentiation between populations within groups, FST: differentiation between populations among groups. Figure S4. Ultrametric tree obtained for Ochthebius haplotypes using the program BEAST (see text for details of the calibration used). Node labels, mean estimated age (MY) and 95% confidence interval (in parenthesis). See Fig. 1 and Table S1 for the codes of the localities and river basins. Figure S5. Ultrametric tree obtained for Nebrioporus haplotypes using the program BEAST (see text for details of the calibration used). Node labels, mean estimated age (MY) and 95% confidence interval (in parenthesis). See Fig. 1 and Table S1 for the codes of the localities and river basins.