2064.62442
Ofir Pele & Liat Bar
CS-LS 2nd year, Hebrew University of Jerusalem, Israel
System Biology
Abstract
System Biology is a new and developing field of research in
bioinformatics which deals with analyzing large scale systems of biology.
In contrast to the old approach of biology which looked only on few
interactions and factors.
We will review two such systems, and the methods that were used to
study and analyze them.
1. Metabolic Network - the galactose pathway1.
In this system the researchers demonstrate an integrated approach to
build, test, and refine a model of a cellular pathway, in which
perturbations to critical pathway components are analyzed using DNA
microarrays, quantitative proteomics, and databases of known physical
interactions.
Using this approach the researchers expand the basic galactose pathway by:
1. Identifying 997 mRNAs that are related to the galactose pathway.
2. Providing evidence that 15 proteins are regulated posttranscriptionally.
3. Identifying explicit physical interactions governing the cellular response to
each perturbation.
2. A Genomic Regulatory Network for Development of the sea urchin
endomesoderm2.
The development of the body plan is controlled by large networks of
regulatory genes. A gene regulatory network that controls the
specification of endoderm and mesoderm in the sea urchin embryo was
summarized in this article.
The network was derived from large-scale perturbation analyses, in
combination with computational methodologies, genomic data, cisregulatory analysis, and molecular embryology.
We will review the biological & computational methodologies that were
used in this research and especially the approach of looking at the
network of genes as if it was a digital circuit ( mitug circuit).
1
Integrated genomic and proteomic analyses of a systematically
perturbed metabolic network. Science. 2001 May 4; 292(5518):929-934.
2
Genomic Regulatory Network for Development
Davidson EH, et al. Science 2002 295(5560):1669-1678. Review