National Research Program of Genomic Medicine
Microarray & Gene Expression Analysis Core Facility
Affymetrix Chips
Sample Submission Form
By
NRPGM Microarray & Gene Expression Core Facility
Version 1.2
June /2004
Project Code: ___________
Sample Submission Form
(please fill the areas below)
PI’s Name: _________________
Name: ________________ Department: ______________Institute: _______________
Phone: ________________ Fax: ________________
Email: ____________________
Address: ________________________________________________________________
Name of the sample(s): ___________________________________________________
Sample type: □Total RNA □ mRNA □ cRNA
Service ID/Array type: ____________________________________________________
Date of sample submission: _______________________________________________
(http://genmed.sinica.edu.tw/microarray/servicelist)
RNA quantification and quality control performed by the customer:
The criteria of RNA integrity and quantity was shown on
http://genmed.sinica.edu.tw/ microarray/RNA_QC.html

RNA Integrity Test:
□ Electrophoresis gel result of the RNA sample(s)
□ Agilent 2100 Bioanalyzer graphic result
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Please paste your image here
For Electrophoresis:
Please refer to the image at the left for the layout of the
gel
Lane 1:100 bp DNA ladder
Lane 2: acceptable quality
Lane 3: heavily degraded total RNA
Lane 4: slightly degraded and DNA- contaminated
sample
Lane 5: 1 Kb DNA ladder
For Bioanalyzer:
Please paste the graphic result to show there is no
degradation.
** The 28S and 18S eukaryotic ribosomal RNAs should exhibit a near 2:1 ratio. In RNA
samples that have been degraded, this ratio will be reversed since the 28S rRNA.

For electrophoresis, the O.D. A260 /A280 ratio should be close to 2.0 for pure RNA (ratios between 1.9
and 2.1 are acceptable).

For 2100 Bioanalyzer, the ratio of 28S/18S rRNA should be more than 2* w/o degradation. (*By case)
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Project Code: ___________
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RNA quantity and purity test

At least 20μg total RNA or 2μg mRNA is required for each chip.
Total RNA concentration should be over 1 μg/μl

Sample ID
Concentration OD260/OD280 Ratio 28S/18S rRNA ratio
(μg/μl)
(for spectrometer)
(for Agilent system)
P.I. Signature:
Comment: (official only)
___
Approval □YES □NO
Signature: ___
Sample transportation:

Deliver the sample in a dry iced-container, together with [Sample Submission Form] to
台北市北投區 112 石牌立農街二段 101 號 2F(陽明大學致和新村 101 號)
「微陣列與基因表現分析核心設施」收

Please inform us the delivery by phone (02) 2826-7337 (02) 2826-7000 ext 5125
by fax (02) 2826 1444, or by e-mail to nrpgm_ec@ym.edu.tw
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