Environmental Health and Safety Department
125 South Fort Douglas Blvd Bldg 605 SLC, UT 84113 (801)581-6590
Biosafety Level 2+ Lab Audit Form
Date:
Location:
PI Name & IBC Protocol #:
Department:
Lab Contact:
Email:
Phone:
EHS Specialist:
Major violations must be reported to PI and addressed and corrected
promptly. Minor violations must be addressed within 30 days.
PASS
BSL-2+ Lab Environment
BSL-2+ Hazard Warning signs properly posted at entry to BSL2+ area.
IBC Approved Protocol, SOP’s, and Spills & Exposure
Procedure available in the BSL-2+ area.
BSL-2+ area has restricted access by door. Window in door?
Lab door remains closed at all times.
BSL-2+ area is clean and orderly.
Windows in BSL-2+ area do not open to exterior.
BSL-2+ Lab Equipment
Biological safety cabinet and clean bench certification current?
Staff is aware of proper use / limitations of BSC (air flow
disturbance, volatile/flammable chemical use, etc.)
BSL-2+ & aerosol generating activities are performed in the
BSC? (Sonicating, homogenizing, vortexing, loading &
unloading centrifuge cups, etc.).
Vacuum lines protected with liquid disinfectant traps; HEPA
filter between vacuum line and liquid trap?
Liquid disinfectant traps properly labeled?
Emergency shower, eyewash within 100 ft. Test current?
Centrifuge visually inspected before use and logs current.
Safety cups or sealed rotor heads used?
BSL-2+ area has sink, soap, and paper towels.
First aid kit readily accessible and adequately stocked.
Bench tops are impervious to water.
Chairs have surfaces for easy cleaning and decontamination.
No cloth chairs.
If present, is Autoclave inspection current?
Revised 4/24/2015
FAIL
N/A
Comments
Disinfectants/cleaning materials available in BSL-2+ area?
Properly and clearly labeled?
Filtered pipette tips available, stocked, and kept in BSL-2+ area.
Sharps should not be used under BSL-2+ containment
procedures.
Biohazard signs on storage freezer(s), refrigerator(s),
incubator(s), transport containers.
Exposure Control
Gloves, gowns/lab coat, eye protection, masks (as needed) are
available, stocked, and kept in BSL-2+ area.
Appropriate PPE is worn by all personnel.
Potentially infectious material is decontaminated prior to
disposal.
Potentially infectious material is placed in a leak-proof sealable
secondary container.
Smoking, chewing gum, handling contact lenses, applying
cosmetics is not allowed in lab. No food or drinks consumed or
stored in the lab.
Policy for safe handling and disposal of sharps are instituted
(No recapping of needles, pipets disposed of as sharps).
Laboratory personnel are offered / receive appropriate
immunizations (e.g. Hepatitis B).
Biohazard spill procedures are in place and lab staff is trained.
Administrative Controls
Documented training records: Biosafety/BBP/Shipping (If
required)
Current U of U Biosafety Manual is accessible to staff in lab?
(Electronic or print copy).
Process for inventory control is in place; stocks/cultures are
documented and labeled.
Animals
Are animals administered BSL-2+ agents in approved ABSL-2+
locations? Specify rooms.
ABSL-2+ Hazard Warning signs properly posted at entry to
ABSL-2+ area.
PSDS posted in ABSL-2+ animal care areas where applicable.
Revised 4/24/2015
Notes:
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http://www.ehs.utah.edu
Revised 4/24/2015