Jordan University of Science and Technology
Faculty of Pharmacy
Department of Medicinal Chemistry and Pharmacognosy
First Semester 2008/2009
Course Syllabus
Course Information
Course Title
Pharmaceutical Instrumental Analysis
Course Code
Phar.226
Prerequisites
Phar. 225
Course Website
Instructor
Dr. Adnan M. Massadeh
Office Location
L1 P1 Faculty of Pharmacy
Office Phone #
23545
Office Hours
10:00-11:00 Sunday, Tuesday and Thursday at Faculty of Graduate Studies
10:00-11:00 Monday and Wednesday at Faculty of Pharmacy
Any time if possible.
E-mail
massadeh@just.edu.jo
Teaching Assistant(s)
None
Course Description
The purpose of this course is to provide a basic understanding of the principles, instrumentation and
applications of chemical analysis This course deals with the Analytical Measurements and concerned
with a wide variety of instrumentation and quality control fields in particular in Analytical and
Pharmaceutical Chemistry sciences. The requirements for instrumentation will be discussed during this
course. These includes: the precision, accuracy, sensitivity, selectivity, detection limit, dynamic range,
speed of analysis, cost, safety and automation. It is very important information for our students who
studying the instrumental Analysis to teach how to identify the chemical structure from the
complementary information afforded by four types of spectra: UV, IR, NMR and MS. So, this course will
cover these four instrumentations in details including theoretical background, Applications and solve
problems. Additionally, the introduction to chromatographic theory, separation techniques and
applications concerned on HPLC and GC.
Textbook
Title
Principles in Instrumental Analysis
Author(s)
Skoog, Holler, Nieman
Publisher
Saunders College Publishing
Year
1998
Edition
5th edition
http//www.hbcollege.com
Book Website
Other references
1.David G. Watson, Pharmaceutical Analysis, Churchill Livingston, 1999.
2. G.D.Christian & J.E.O’Reilly : Instrumental Analysis, 2nd edition, Allyn &
Bacon Inc., 1986.
3. A.H.Beckette & J.B.Stenlake : Practical Pharmaceutical Chemistry, 4th
edition, part Two, Athlone 1988.
4. M.H.Gordon & R Macrea : Instrumental Analysis in the Biological Sciences
Blackie & Son Ltd., 1987.
5. G.Christian : Analytical Chemistry, 5th edition, John Wiley & Sons, Inc.,
1994.
6. R.M.Silverstein, G.Clayton Bassler & T.C.Morrill : Spectrometric
Identification of Organic Compounds, 5th edition, John Wiley & Sons,
Inc.,1991.
7. Handouts from different sources.
Assessment
Assessment
Expected Due Date
Percentage
First Exam
Monday, 10-11- 2008
30%
Second Exam
It will be defined after the first exam.
30%
Final Exam
40%
Assignments
-
Participation
-
Attendance
-
Course Objectives
Percentage
1. To provide a background in Instrumental principles that are important in analytical
20%
chemistry, pharmacy, agriculture and other branches of Science.
2. To know the importance of application of instrumental methods in different fields of
25%
science.
3. To differentiate between non-separative and separative instruments.
15%
4. To study the UV, Visible and to solve problems using Beer's-Lambert's law.
20%
5. To know the importance of instrumental analysis in the identification of the
10%
chemical structures using IR, NMR and MS.
6. To teach the students the instrumental techniques used to separate more than one
10%
component such as HPLC and GC.
Teaching & Learning Methods
1. Overview the lectures using the overhead projector.
2. Illustrate the importance concepts on the board.
3. Solve the problems that are related to the course.
4. Discuss and ask the students some question in order to activate the lecture by participating the students
to answer the questions.
5. Give a summary for the lecture.
6. Encourage the students to prepare an assignment related to the course using the internet.
7. Encourage the student to ask any question during the office hours or any time if possible.
Learning Outcomes: Upon successful completion of this course, students will be able to
Related Objective(s)
Reference(s)
1)
Know the importance of the
instrumental analysis in different
aspects of science.
2)
To know the spectral methods of
analysis & their applications.
3)
To understand information about
Ultra-violet UV & Visible
Sperctrophotometric methods
(UV-region, visible region,
To solve problems on Beer'sLambert's Law.
To differentiate between nonseparative and separative
instruments.
4)
5)
Chapter 1 and Handouts
Handout
CH.13 and(handouts)
CH.9,CH.13 and(handouts)
Handout
To know the importance of
instrumental analysis in the
identification of the chemical
structures using IR, NMR and
MS.
To understand the
Chromatography, Theory,
Mechanisms & Techniques such
as HPLC and GC.
6)
7)
CH.16, CH. 17, CH. 19, CH. 20
and Handout
CH.26 and Handout
Useful Resources
1. Jeremy Sanders and Brian Hunter, Modern NMR Spectroscopy: A guide for Chemists, 2 nd edition, Oxford
University Press, 1997.
2. A. Pryde and M. T. Gilbert, Applications of High Performance Liquid Chromatography, Chaoman and Hall,
London,1979.
3. Grinville Holland and Andrew N. Eaton, Applications of Plasma Source Mass Spectrometry II, Royal
Society of Chemistry, 1993.
4. R. M. Silverstien, G. Clayton Bassler, Ternce C. Morrill, Spectrometric Identification of Organic
Compounds, 5th edition., JOHN WILEY and SONS, New York.
5. Analytical Measurements and Instrumentation, Paul N. Cheremisionoff, Harlan J. Perlis, ANN ARBOR
SCIENCE PUBLISHERS, 1982.
Course Content
Week
1
2
3
4+5
Topics
1. Introduction to Instrumental Methods of
Analysis & Quality Control.
2. Spectrometry: an introduction to spectral
methods of analysis & their applications to
pharmaceutical preparations.
3. Atomic Absorption Spectrometry
Chapter in Textbook
(handouts)
CH. 1
(handouts)
(handouts)
CH. 9 + CH. 13
(handouts)
4. Ultra-violet UV & Visible Sperctrophotometric
methods.
CH. 13
(handouts)
6
6. Applications on Beer's-Lambert's Law.
(handouts)
7+8
7. Infrared Spectroscopy (IR), & uses in
Pharmacopoeias.
CH. 16
(handouts)
9
8. Nuclear Magnetic Resonance Spectroscopy
(NMR),
CH. 19
(handouts)
10
9. Applications & uses in the identification of
organic compounds.
11
10. Mass Spectrometry (MS) & its applications
in quantitative & qualitative analytical methods.
CH. 20
(handouts)
12
11. Chromatography: Theory, Mechanisms &
Techniques.
CH.26
(handouts)
12. Liquid Chromatography.
CH.28
(handouts)
13
13. Gas Chromatography
Additional Notes
The Attendance of lectures is important according to the University Regulations.
CH. 17+ CH. 19
(handouts)
CH.27 (handouts)