Person Specification
van Heyningen Group CDF post on NIH grant
Criteria
identified:
Education, qualifications and
training
Previous work experience
Criteria 1:
Creating and producing
constructs
Criteria 2:
Transgenic animal
generation (mouse and
perhaps zebrafish)
Criteria 3:
Analysis of regulatory region
function
Criteria 4:
Cell culture work
Criteria 5:
Chromatin organisation
studies
Essential
Desirable
A good degree in
biological/biomedical
sciences and a higher
degree
Laboratory experience in
two or more of the
following: molecular
biology/genetics;
transgenic animal work;
gene regulation;
chromatin biology
PhD in a relevant field
is highly desirable
Understanding of
principles for making
reporter transgene
constructs, including BAC
engineering
Insight into methodology;
willingness to acquire
skills and obtain personal
licence. Understanding
responsibilities of working
with animals.
Selection of regions for
study – understanding
and developing criteria.
Awareness of how
differentiated cell lines
are created to study
function: iPS cells,
targeted ES cells,
immorto cell lines
Knowledge of methods
for study of DNA binding,
chromatin organisation
and long range
interactions (ChIP, 3C,
5C, DNase
hypersensitivity
Experience in
utilisation of
bioinformatics
information e.g. to
define DNA binding
targets; chromatin
immunoprecipitation;
histological analysis
Experience in making
and engineering
complex constructs
Experience in making
transgenic animals
Insight into how
regulatory function is
dissected, using: 1.
transgenics; 2. direct
targeted deletion; 3.
mutant rescue
Experience of ES cell
culture and
manipulation.
Experience of these
techniques for
chromatin analysis
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh
EH4 2XU
Tel: +44(0)131 332 2471 fax +44(0)131 467 8456
www.hgu.mrc.ac.uk
Person Specification
Criteria 6:
Bioinformatics
Knowledge of
bioinformatics tools and
usage
Familiarity with R and
bioconductor packages
for statistical analysis
of biological data.
Advanced comparative
sequence analysis.
PERL programming.
Criteria 7:
Scientific communication
The ability to present
research data in a clear
and coherent manner
The ability to write a
scientific paper
Experience with Adobe
Illustrator or similar
package for
constructing highquality figures.
Criteria 8:
Information management
Basic database
management. Secure
storage of data with full
experimental details
Database
programming. High
level data
management skills
Personal style and behaviour:
We are looking for someone who combines an enthusiastic approach to scientific
projects with persistence and the ability to complete projects and publish in a
timely manner. The ability to work in a group setting is crucial.
Additional information:
In this field it is important to be able to learn new techniques quickly and work to
a very high standard.
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh
EH4 2XU
Tel: +44(0)131 332 2471 fax +44(0)131 467 8456
www.hgu.mrc.ac.uk