TABLE S4: Sodium butyrate induced 17Syn+ downregulation of twenty-four genes
Gene nameNetAffx
designation
Gene symbol
(description)
Gene function
Dmbt1 (deleted in
malignant brain tumors 1)
immune activity,
-11.06 (ND)*
interaction of
tumor cells and the
immune system
220133_at
(NM_017855.1)
Odam (odontogenic,
ameloblast asssociated)
biomineral
formation
-10.7 (ND)
1417160_s_at
(NM_007969.1)
Expi (extracellular
proteinase inhibitor)
peptidase inhibitor
activity
-7.6 (ND)
1422448_at
(NM_009363.1)
Tff2 [trefoil factor 2
(spasmolytic protein 1)]
may protect the
mucosa from
insults, stabilize
the mucus layer
and affect healing
of the epithelium
-7.0 (ND)
1421522_at
(NM_008081.1)
B4galnt2 (beta-1,4-Nacetyl-galactosaminyl
transferase 2)
negative regulation -6.2 (ND)
of cell-cell
adhesion;
lipid glycosylation
1450060_at
(NM_011082.1)
Pigr (polymeric
immunoglobulin receptor)
receptor activity,
-5.6 (ND)
binds polymeric
IgA and IgM at the
basolateral surface
of epithelial;
protein binding
1449452_a_at
(NM_025989.1)
Gp2 [glycoprotein 2
(zymogen granule
membrane)]
electron carrier
activity
-5.3 (ND)
1439423_x_at
(AV089660)
U46068 (cDNA sequence
U46068)
lipid binding
-5.1 (ND)
Fold change Microarray
(qPCR)
(Genbank
accession
number)
1418287_a_at
(NM_007769.1)
1423719_at
(U46068.2)
LOC632073; U46068
(similar to long palate,
lung and nasal epithelium
carcinoma associated 1
isoform 2; cDNA
sequence U46068)
lipid binding
-4.5 (ND)
1454254_s_at
(AK002767.1)
1600029D21Rik (RIKEN
cDNA 1600029D21 gene)
Unknown
-4.4 (ND)
1449184_at
(NM_009402.1)
Pglyrp1 (peptidoglycan
recognition protein 1)
apoptosis;
immune response,
may kill Grampositive bacteria
by interfering with
peptidoglycan
biosynthesis and
has bacteriostatic
activity towards
Gram-negative
bacteria; innate
immunity
-4.3 (ND)
1416040_at
(NM_026334.1)
Lipf (lipase, gastric)
lipid metabolism;
protein binding;
malate
dehydrogenase
activity;
hydrolase activity
-4.1 (ND)
1427626_at
(AF369933.1)
Muc5b (mucin 5, subtype
B, tracheobronchial)
1418818_at
(NM_009701.1)
Aqp5 (aquaporin 5
similar to aquaporin 5)
gel-forming
-4.0 (ND)
mucin, contributes
to the lubricating
and viscoelastic
properties of
whole saliva and
cervical mucus.
transporter
-3.9 (ND)
activity;
protein binding;
water channel
activity, implicated
in the generation
of saliva, tears,
and pulmonary
secretions
1451498_at
(BC004853.1)
Lrrc26 (leucine rich repeat protein binding
containing 26)
1450009_at
(NM_008522.1)
Ltf (lactotransferrin )
iron ion binding;
-3.5 (ND)
protein binding;
ferric iron binding;
peptidase activity;
metal ion binding;
regulation of iron
homeostasis; host
defense against a
broad range of
microbial
infections; antiinflammatory
activity; regulation
of cellular growth
and differentiation;
protection against
cancer
development and
metastasis
1424351_at
(AF334269.1)
Wfdc2 (WAP fourdisulfide core domain 2)
Serine-type
endopeptidase
inhibitor activity;
peptidase inhibitor
activity; protease
inhibitor
-3.3 (ND)
1419028_at
(NM_033264.1)
Arpp21 (cyclic AMPregulated phosphoprotein,
21)
nucleic acid
binding; cellular
response to heat;
competitive
inhibitor of
calcineurin in
neurons
-3.3 (ND)
-3.9 (ND)
1420816_at
(NM_018871.1)
Ywhag (tyrosine 3monooxygenase/tryptopha
n 5-monooxygenase
activation protein, gamma
polypeptide)
protein targeting
-3.3 (ND)
regulation of
neuron
differentiation;
regulation of
synaptic plasticity;
actin binding;
protein kinase C
binding;
insulin-like growth
factor receptor
binding;
protein binding;
protein domain
specific binding
1449838_at
(NM_009639.1)
Crisp3 (cysteine-rich
secretory protein 3)
1442333_a_at
(AI549833)
Scn9a (sodium channel,
voltage-gated, type IX,
alpha)
innate immunity,
major secretory
product of
macrophages
ion transport;
inflammatory
response;
post-embryonic
development
regulation of
action potential in
neuron;
inflammatory pain
mechanisms
1424502_at
(BC024542.1)
Oit1 (oncoprotein induced
transcript 1)
negative regulation -3.1 (ND)
of insulin secretion
1423933_a_at
(BC022950.1)
1600029D21Rik (RIKEN
cDNA 1600029D21 gene)
unknown
-3.1 (ND)
1433987_at
(AV153557)
Hpcal4 (hippocalcin-like
4)
calcium channel
regulator activity;
calcium ion
binding;
protein C-terminus
binding protein
domain specific
-3.0 (ND)
-3.2 (ND)
-3.1 (ND)
binding
Fold change values of expression of the twenty-four genes based on microarrays in the 17Syn+
high phenotypic reactivator latent TG relative to its mutant LAT negative recombinant, 17Pst
low phenotypic reactivator latent TG 1 hour after NaBu i.p. of mice. Relative quantitative
expression levels were determined for each of the seventeen genes. All results are expressed as
an expression ratio of the latent 17Syn+ latent TG to the 17Pst(LAT-) latent TG of NaBu-treated
mice, normalized against β-actin expression levels using the 2-CT method (Livak and
Schmittgen, 2001). *Not determined.