NORTH CAROLINA STATE UNIVERSITY GRADUATE COURSE ACTION FORM NOTE: Click once on shaded fields to type data. To check boxes, right click at box, click “Properties”, and click “Checked” under Default Values. TYPE OF PROPOSAL DEPARTMENT/PROGRAM PLANT PATHOLOGY COURSE PREFIX/NUMBER PP (MB) 715 PREVIOUS PREFIX/NUMBER PP 610W/810W DATE OF LAST ACTION NA COURSE TITLE Applied Evolutionary Analysis of Population Genetic Data ABBREVIATED TITLE APPL EVOL POPN GEN SCHEDULING Fall Spring Alt. Year Odd COURSE OFFERED CREDIT HOURS CONTACT HOURS Studio New Course Drop Course Course Revision Dual-Level Course REVISION Content Prefix/Number Title Abbreviated Title Credit Hours Contact Hours Grading Method Pre-Corequisites Restrictive Statement Description Scheduling Summer Every Year Alt. Year Even Other BY DISTANCE EDUCATION ONLY ON CAMPUS ONLY BOTH ON CAMPUS AND BY DISTANCE EDUCATION 3 Lecture/Recitation 2 Seminar Independent Study/Research Laboratory Problem 1 Internship/Practicum/Field Work GRADING ABCDF S/U INSTRUCTOR (NAME/RANK) Ignazio Carbone/Assistant Professor Graduate Faculty Status Associate ANTICIPATED ENROLLMENT Per semester 15 PREREQUISITE(S) PP 707, GN 703, ST 501 COREQUISITE(S) type course numbers here PRE/COREQUISITE FOR type course number(s) here RESTRICTIVE STATEMENT type BRIEF statement here CURRICULA/MINORS Required Qualified Elective type program name here type elective name here PROPOSED EFFECTIVE DATE August 2007 Max.Section 20 Full Multiple sections Yes No APPROVED EFFECTIVE DATE __________________________ CATALOG DESCRIPTION (limit to 80 words): This course will introduce nonparametric and model-based methods for making inferences on population processes (mutation, migration, drift, recombination, and selection). The goal is to provide a conceptual overview of these methods and hands-on training on how to implement and interpret the results. Sample data sets in computer laboratories will integrate summary statistic, cladistic, coalescent, and bayesian approaches to examine population processes in different pathosystems with specific emphasis on eukaryotic microbes, viruses and bacteria. DOCUMENTATION AS REQUIRED Please number all document pages RECOMMENDED BY: _______________________________________________________________________ Department Head/Director of Graduate Programs Date Course Justification Proposed Revision(s) with Justification Student Learning Objectives Enrollment for Last 5 Years ENDORSED BY: _______________________________________________________________________ Chair, College Graduate Studies Committee Date New Resources Statement Consultation with other Departments Syllabus (Old and New) Explanation of differences in requirements of dual-level courses ________________________________________________________________________ College Dean(s) Date APPROVED: ______________ _______________________________________________________ 1 ____ Dean of the Graduate School Date 2 COURSE JUSTIFICATION Genomes are dynamic and evolving units. Their present organization and function is the result of evolutionary forces that are acting on individuals in populations over time. This course is designed to provide an advanced understanding of evolutionary tools, their specific assumptions, limitations and power. Some of the topics covered include molecular marker selection and their utility in evolutionary analysis, approaches for biological data integration and analysis, cladistic methods for biological inference, population parameter estimation and hypothesis testing using summary statistic, coalescent and Bayesian models. This course will be organized into lectures and computer laboratories (problem periods). Lectures will introduce evolutionary concepts and laboratories will provide hands-on training with the tools. The focus will be on applying these concepts and tools to understand evolutionary processes in eukaryotic microbes, viruses and bacteria. Students will have the opportunity to analyze their own molecular data sets or work through previously published data sets using new tools and methodologies. The evolutionary approaches that will be examined in this course are inherently rich in mathematical concepts, statistics and population genetics theory. Students will gain a better understanding of these concepts by applying these methods to real data sets. STUDENT LEARNING OBJECTIVES This course will provide students with: 1. Hands-on training on a diverse set of tools implemented in the SNAP Workbench evolutionary toolkit (http://snap.cifr.ncsu.edu). Among these are Hudson’s haplotype and sequence-based statistics, MDIV and IM (isolation models), migrate-n (island models), Genetree (infinite sites and stepwise mutation models), RecMin, recom58, fins, LDhat, shrub, HapBound, beagle, and LAMARC. 2. Step-by-step analysis and interpretation of sample data sets. opportunity to analyze their own data set. 3. A foundation in the use of SNAP Workbench to allow for further integration of new methods for estimating population parameters. 4. An understanding of the power, limitations and assumptions of summary statistic, cladistic, coalescent, and bayesian approaches in evolutionary population genetic analysis. 5. Criteria for molecular marker selection and their utility in evolutionary analysis. 6. Genealogical-based approaches for integrating molecular genetic and phenotypic data. 7. Experience in data analysis, interpretation, written and oral presentation. Students will have the 2 SYLLABUS PP715-Applied Evolutionary Analysis of Population Genetic Data Fall 2007 Location: Room 105b located in Toxicology Building, Centennial Campus Meeting Day and Time: Friday, 9:00AM-12:00PM Instructor: Dr. Ignazio Carbone E-mail: ignazio_carbone@ncsu.edu Office: Partners III Building, Room 229 Phone: 513-4866 Office Hours: By appointment Prerequisites: PP707, GN 703, ST 501 REQUIRED TEXT No text is required for this course. Suggested readings will be made available for each lecture topic online by logging into the Vista site (http://vista.ncsu.edu). Copies of the syllabus and class notes will be available in the classroom and online after the lecture. GRADING One Exam One 15 min Oral Presentation One Research Paper Class Participation and Discussion 30% 30% 30% 10% Oral presentations will allow students to build on their knowledge of concepts introduced in lectures and labs by analyzing a published dataset (provided by the instructor) for the presence and magnitude of a specific evolutionary force. This will require students to integrate nonparametric and parametric methods implemented in the workbench and to interpret their results with specific emphasis on the biological inferences and the assumptions and limitations of the selected methods. Research papers will require students to integrate all tools and methodologies presented in the course to analyze their own molecular dataset or select one provided by the instructor. Research papers will follow scientific format and include tables, figures and references. FINAL GRADES Final grade submitted will be based on the following grade scale; 100-98=A+, 97.9-91=A, 90.989=A-, 88.9-87=B+, 86.9-81=B, 80.9-79=B-, 78.9-77=C+, 76.9-71=C, 70.9-69=C-, 68.9-67=D+, 66.9-61=D, 60.9-59=D-, Below 59=F 4 ATTENDANCE POLICY Attendance will not be taken. Students are responsible for attending and for learning the material provided in both lecture and computer laboratory. If a class is missed, it is the students' responsibility to obtain lecture notes and handouts on their own. The instructor will not provide lecture notes to students. ACADEMIC INTEGRITY All students are required to adhere to the NC State University's Code of Student Conduct. The text of this code may be obtained from the instructor or is available online at: (http://www.ncsu.edu/student_conduct). STATEMENT FOR STUDENTS WITH DISABILITIES: " Reasonable accommodations will be made for students with verifiable disabilities. In order to take advantage of available accommodations, students must register with Disability Services for Students at 1900 Student Health Center, Campus Box 7509, 515-7653. For more information on NC State's policy on working with students with disabilities, please see the Academic Accommodations for Students with Disabilities Regulation (REG02.20.1): http://www.ncsu.edu/policies/academic_affairs/courses_undergrad/REG02.20.1.php ENROLLMENT LAST 5 YEARS This course was taught in Fall 2003 and Fall 2005 as a Special Topics Course. Fall 2003 – PP610W/810W – 1 credit hour – 8 students enrolled Fall 2005 – PP610W/810W – 3 credit hours – 13 students enrolled (10 PhD students) NEW RESOURCES REQUIRED No new resources required. Computer lab in Toxicology 105b is available for use. 5 PP715 Schedule Lecture Topic Suggested Reading 1, 2 Introduction: Theoretical Population Genetics with focus on eukaryotic microbes, viruses and bacteria Wakeley (2005) Pluzhnikov & Donnelly (1996) 3, 4 Population Structure I: Evolutionary Framework Hey and Machado (2003) Price and Carbone (2005) 5, 6 Population Structure II: Summary Statistics Neutrality Tests a priori Inference Tajima (1989a) Tajima (1989b) Fu and Li (1993) Fu (1997) 7, 8 Population Structure III: Intraspecific Phylogenetic Reconstruction Compatibility and Recombination Blocks Network Inference Koopman (2005) Cassens et al. (2005) Jakobsen et al. (1997) Myers and Griffiths (2003) Exam, 9 Population Structure IV: Nested Cladistic Analysis Phylogeography Haplotype-phenotype Association Biological Inference Knowles & Maddison (2002) Hudson et al. (1992) Templeton (1998) Hare (2001) 10, 11 Population Processes I: Coalescent Models (panmixia, recombination) Genealogical Inference Hudson (1990) Rosenberg & Nordborg (2002) Griffiths & Marjoram (1996) 12, 13 Population Processes II: Coalescent Models (subdivision) Genealogical Inference Bahlo and Griffiths (2000) Carbone et al. (2004) Fall Break 14, 15 Tools for Single Locus Inferences Example from viruses Carbone et al. (2004) 16, 17 Tools for Multilocus Inferences Examples from viruses and fungi Carbone et al. (1999) Carbone et al. (2004) 6 18, 19 Methods in Testing for Population Subdivision Oral Presentations Hudson (2000) 20, 21 Tools for Multilocus Estimates of Recombination Rates Oral Presentations Fearnhead and Donnelly (2001) 22, 23 Multilocus Estimates of Migration Rates, Effective Population Sizes and Divergence Time Oral Presentations Hey and Nielsen (2004) 24, 25 Simultaneous Estimation of Migration and Recombination Oral Presentations Beerli and Felsenstein (2001) Thanksgiving Holiday – No class 26 Synthesis and Course Evaluation Research Paper Due 7 From: Gerry Luginbuhl <Gerry_Luginbuhl@ncsu.edu> Date: Tue, 20 Dec 2005 16:41:12 -0500 To: <ignazio_carbone@ncsu.edu> Subject: PP710 Ignazio, The Graduate Curriculum Committee in the Department of Microbiology has reviewed the syllabus for PP710 and your request for cross-listing this course in Microbiology. From the syllabus provided, the committee viewed the content of the course as focusing on the computational methods of evolutionary analysis of genomes. This is a relevant topic for Microbiology students to pursue and given the breadth of interests in the graduate program it would be a suitable elective course for some students. However, while the example course study papers you provided were interesting and relevant, it is the syllabus and course description that are first available to prospective students for determining the focus and relevance of the course. To better assist Microbiology graduate students in appreciating what you identified as the skew of the course in applying these concepts to eukaryotic microbes, viruses, and even bacteria, these elements could be presented in the course description and in the syllabus. With that recommendation, the Department of Microbiology approves the cross-listing of this Ph.D. level course. You should be aware that MB 710, Biological Transmission Electron Microscopy (an "on demand" graduate course) currently uses the 710 designation so this must be considered for the MB cross-listing. Gerry Gerry Luginbuhl Interim Head Department of Microbiology North Carolina State University Raleigh, NC 27695-7615 Phone: 919-515-7484 8 ------ Forwarded Message From: Margaret Daub <margaret_daub@ncsu.edu> Reply-To: <margaret_daub@ncsu.edu> Date: Wed, 21 Dec 2005 11:29:14 -0500 To: Ignazio Carbone <ignazio_carbone@ncsu.edu>, <james_moyer@ncsu.edu> Cc: <boston@unity.ncsu.edu> Subject: Re: PP 710 Ignazio and Jim – We are pleased to see the new plant pathology course offering on "Applied Evolutionary Analysis of Population Genetic Data". It will be an attractive offering for students interested in population biology. The Department of Botany does not offer any similar courses. We anticipate that it would be taken by a few of our students, and that interest may increase as collaborations are established among some of our recent hires. We support the addition of this course to the CALS curricula. We do not anticipate a need to crosslist the course with Botany at this time. Thank you for sharing the information about this course. Regards - Margo *************************** Margaret E. Daub Professor and Department Head Department of Botany Box 7612 North Carolina State University Raleigh, NC 27695-7612 Phone: (919) 515-2727, 515-6986 FAX: (919) 515-3436 E-mail: margaret_daub@ncsu.edu *************************** 9 ------ Forwarded Message From: Zhao-Bang Zeng <zeng@statgen.ncsu.edu> Date: Fri, 06 Jan 2006 15:30:32 -0500 To: Ignazio Carbone <ignazio_carbone@ncsu.edu> Cc: Barbara Sherry <barbara_sherry@ncsu.edu> Subject: Re: PP 710 Dear Ignazio: I have forwarded your message to BI curriculum committee and have not gotten feedback yet. This is a very interesting and practical course for evolutionary studies on popgen data. I am sure that some of our BI students would find it useful and your record showed that. BI curriculum has not formally set up yet. We will discuss it as a possible selective course for BI students in due course. Zhao-Bang Zeng *********************************************************************** Dr. Zhao-Bang Zeng William Neal Reynolds Distinguished Professor Director, Bioinformatics Research Center Departments of Statistics & Genetics North Carolina State University 1521 Partners II Building, 840 Main Campus Drive Raleigh, NC 27695-7566, USA Tel: 919 515-1942; FAX: 919 515-7315 (Attn: Zeng) Email: zeng@stat.ncsu.edu; Home page: http://statgen.ncsu.edu/ *********************************************************************** 10