PROTEIN TECHNOLOGY CORE
2D GEL ELECTROPHORESIS SERVICES
Sample Details:
Sample Name:
Sample format (Please select):
Solid /
Liquid
(If liquid give details of buffer composition):
Quantity of protein/sample (mg):
Number of samples
Number of replication per sample:
1/
2/
Is there any potential pathogen in the sample?
Protocol provided:
Y /
3
Y /
N
N
Any special comment/data? (Please attach documents along)
Total protein stain:
Coomassie/
Silver staining
Attach 1D SDS gel analysis data along with gel image (if available):
Y /
N
Please note: Sample preparation will be charged separately, if needed. Please refer sample preparation
guidelines for further details.
Specify pI and molecular weight of protein/s to be analyzed. (If specific protein/s to be
analyzed):
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
Service Details:
Samples are placed in a queue on receipt with the completed form. Reports will be sent in
electronic format (PDF).
2D Gel electrophoresis sample preparation guide lines:

Components to be avoided include ionic detergents (e.g., SDS), Salts, nucleic acids,
lipids and polysaccharides.

Samples can contain reducing agents (e.g., DTT, TBP), IPG buffers (eg. ampholytes,
pharmalytes) and detergents such as NP40 and Triton X-100.

Total salt concentration in the sample should not exceed 10 mM.

Final concentration of SDS should be 0.25% or lower and a neutral or zwitterionic
detergent should be present in at least an 8-fold excess over the SDS (e.g. CHAPS, Triton
X-100).

A sample with low protein concentration and a high salt concentration, could be
desalted using desalting columns (for large sample volumes) then concentrated by
lyophilization, or precipitated with TCA and ice-cold acetone and resolubilized with
rehydration solution.

2D clean up kit is available in market. Sample must be dissolved in appropriate
rehydration buffer. For more details please contact us.

The required protein concentration is at least 5μg/μL and at least 500 μg of each sample
is needed for a single preparative run. (Note: Protein load varies depending on the pH range
of immobilized pH gradient (IPG) strips).

Clinical samples must be pre-processed (as mentioned above) before sample submission.
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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PROTEIN TECHNOLOGY CORE
Terms of service:
If the user wants more parameters, these are to be selected or mentioned. If the user already has
a published protocol that they would like us to follow, please send us a copy of the protocol and
a PDF of the published manuscript if available.
Recommendations and/or modifications to the services described herein can be provided by
the facility.
Deliverables and Payment terms:
a)
Results obtained will be shared with the client. Deliverables (e.g. Analyzed data and gel
pictures) will be provided to the client upon request. If not requested during the service,
deliverables will be provided at the conclusion of the service.
b)
Multiple quotations may be given during the course of the service (dependent on
requirements of client); however a single invoice will be raised for the complete service for
payment by the client.
Publications & Acknowledgements:
In the event that results from the service provided are to be published, depending on the
intellectual input provided by C-CAMP Protein facility, authorship can be discussed. In any
event regardless of authorship, the Protein facility must be acknowledged in any publication of
results from the service.
Disclaimer:
The facility through its expertise and experience will assure the best of services as requested.

Appropriate standards will be used to analyze the protein distribution patterns.
In case of service failures due to unforeseen reasons, client will still be invoiced for the services
rendered.
Centre for Cellular and Molecular Platforms
National Centre for Biological Sciences
GKVK Campus, Bellary Road, Bengaluru 560065, India
Phone: 080 23666001/002, 080 67176001/002
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